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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent显示文摘 | Vernon L. Singleton Rudolf Orthofer Rosa M. Lamuela-Raventós | 1999 | Methods in Enzymology1999，，： | 8 |
| 3 | 同步荧光法检测鱼胆汁中的1-羟基芘显示文摘建立了用同步荧光法测定鱼胆汁中的 1 羟基芘 (1 HP)的方法。该方法通过直接测定鱼胆汁的多环芳烃 (PAHs)的代谢产物之一 1 HP ,用于判断PAHs生物有效部分对海岸带养殖水环境的污染程度。以真鲷(Pargrosomusmajor,简称Pm) , 鱼 (Nibeamiichthys ,简称Nm)为实验对象 。 | 张勇 王淑红 朱亚先 Michael H W Lam Rudolf S S Wu 洪华生 | 2002 | 分析化学2002，30，4： | 6 |
| 4 | A typology for the classification, description and valuation of ecosystem functions, goods and services显示文摘 | Rudolf S de Groot Matthew A Wilson Roelof M.J Boumans | 2002 | Ecological Economics2002，，3： | 3 |
| 5 | Galectin-3 Mediates Aldosterone-Induced Vascular Fibrosis显示文摘 | Laurent Calvier Maria Miana Pascal Reboul Victoria Cachofeiro Ernesto Martinez-Martinez Rudolf A. de Boer Fran?oise Poirier Patrick Lacolley Faiez Zannad Patrick Rossignol Natalia López-Andrés | 2013 | Arteriosclerosis, Thrombosis, and Vascular Biology2013，，1： | 2 |
| 6 | [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent显示文摘 | Vernon L. Singleton Rudolf Orthofer Rosa M. Lamuela-Raventós | 1999 | Methods in Enzymology1999，，： | 2 |
| 7 | Continuum percolation of wireless ad hoc communication networks 显示文摘 | Ingmar G Wolfram K Rudolf S | 2003 | Statistical Mechanics and its Applications2003，325，34： | 1 |
| 8 | A novel hybrid of carbon nanotubes/ iron nanopartieles: iron-filled nodule-containing carbon nanotubes显示文摘 | LIU S W RUDOLF J W | 2005 | Carbon2005，43，： | 1 |
| 9 | Current and future therapies for agerelated macular degeneration显示文摘 | Beutel J Rudolf M Grisanti S | 2009 | Expert Opin Emerg Drugs2009，14，： | 1 |
| 10 | Performance meas- urement of modular product platforms 显示文摘 | SCHUH G RUDOLF S VOGELS T | 2014 | Variety Man- agement in Manufacturing2014，17，： | 1 |
| 11 | Cultured gill epithelial cells from tilapia(Oreochromis niloticus): a new in vitro assay for toxicants显示文摘 | Zhou B S Liu W H Rudolf S S | 2005 | Aquatic Toxicology2005，71，1： | 1 |
| 12 | Evaluation of luciferase reporter bacteriophage A511:luxAB for detection of Listeria monocytogenes in contaminated foods显示文摘 | Loessner M J Rudolf M Scherer S | 1997 | Appl Environ Microbiol1997，63，： | 1 |
| 13 | Proximal humeral fraeture显示文摘 | Rudolf S Wolfgang S Peter S | 1993 | Clin Orthop Relat Res1993，，292： | 1 |
| 14 | Flow-through ultrasonic emulsification combined with static micromixing for aseptic 显示文摘 | Freitas S Rudolf B Merkle H P | 2008 | Eur J Pharm Biopharm2008，61，3： | 1 |
| 15 | Relationship Learning and Performance Enhancement via Advanced Information Technology:The Case of Taiwanese Dragon Electronics firms显示文摘 | JEAN R J B RUDOLF R S | 2010 | International Marketing Review2010，27，2： | 1 |
| 16 | Enterococcus nosus infection in an immunosuppressed patient: case report and review of the lit erature显示文摘 | Bettina SF Georg S Rudolf L | 2005 | Journal of IMection2005，51，27： | 1 |
| 17 | Temperature monitoring of interstitial thermal tissue coagulation using MR phase images显示文摘 | Rudolf S Peter WA Dietrich H | 1997 | JMRI1997，8，： | 1 |
| 18 | Optimization of casting processes-leveraging coupled process simulation and multi-object optimization to the manufacturing level显示文摘 | GOTZ C H VLOKER K RUDOLF S | 2003 | International Congress on FEM Technology2003，，12： | 1 |
| 19 | Two - phase flow ehar- acteristies in gas- liquid microreactors显示文摘 | Waelehli S Rudolf yon Rohr P | 2006 | Internation- al Journal of Multiphase Flow2006，32，： | 1 |
| 20 | Paper electrophoresis gonadoctropic substances in pregnant mares urine显示文摘 | Ludimor I Jozef A Rudolf S | 1965 | Endocrynol Polska1965，16，： | 1 |
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