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| 1 | The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain——an in vitro study of human periodontal ligament fibroblasts显示文摘During orthodontic tooth movement(OTM)mechanical forces trigger pseudo-inflammatory,osteoclastogenic and remodelling processes in the periodontal ligament(PDL)that are mediated by PDL fibroblasts via the expression of various signalling molecules.Thus far,it is unknown whether these processes are mainly induced by mechanical cellular deformation(mechanotransduction)or by concomitant hypoxic conditions via the compression of periodontal blood vessels.Human primary PDL fibroblasts were randomly seeded in conventional six-well cell culture plates with O2-impermeable polystyrene membranes and in special plates with gas-permeable membranes(Lumox?,Sarstedt),enabling the experimental separation of mechanotransducive and hypoxic effects that occur concomitantly during OTM.To simulate physiological orthodontic compressive forces,PDL fibroblasts were stimulated mechanically at 2 g·cm?2 for 48 h after 24 h of pre-incubation.We quantified the cell viability by MTT assay,gene expression by quantitative real-time polymerase chain reaction(RT-qPCR)and protein expression by western blot/enzyme-linked immunosorbent assays(ELISA).In addition,PDL-fibroblast-mediated osteoclastogenesis(TRAP+cells)was measured in a 72-h coculture with RAW264.7 cells.The expression of HIF-1α,COX-2,PGE2,VEGF,COL1A2,collagen and ALPL,and the RANKL/OPG ratios at the mRNA/protein levels during PDL-fibroblast-mediated osteoclastogenesis were significantly elevated by mechanical loading irrespective of the oxygen supply,whereas hypoxic conditions had no significant additional effects.The cellular–molecular mediation of OTM by PDL fibroblasts via the expression of various signalling molecules is expected to be predominantly controlled by the application of force(mechanotransduction),whereas hypoxic effects seem to play only a minor role.In the context of OTM,the hypoxic marker HIF-1αdoes not appear to be primarily stabilized by a reduced O2 supply but is rather stabilised mechanically. | Niklas Ullrich Agnes Schroder Jonathan Jantsch Gerrit Spanier Peter Proff Christian Kirschneck | 2019 | International Journal of Oral Science2019，11，4： | 5 |
| 2 | Regular nicotine intake increased tooth movement velocity,osteoclastogenesis and orthodontically induced dental root resorptions in a rat model显示文摘Orthodontic forces have been reported to significantly increase nicotine-induced periodontal bone loss. At present, however, it is unknown, which further(side) effects can be expected during orthodontic treatment at a nicotine exposure corresponding to that of an average European smoker. 63 male Fischer344 rats were randomized in three consecutive experiments of 21 animals each(A/B/C) to 3 experimental groups(7 rats, 1/2/3):(A) cone-beam-computed tomography(CBCT);(B) histology/serology;(C) reversetranscription quantitative real-time polymerase chain reaction(RT-q PCR)/cotinine serology—(1) control;(2) orthodontic tooth movement(OTM) of the first and second upper left molar(Ni Ti closed coil spring, 0.25 N);(3) OTM with 1.89 mg·kg^(-1) per day s.c. of L(-)-nicotine. After 14 days of OTM, serum cotinine and IL-6 concentration as well as orthodontically induced inflammatory root resorption(OIIRR), osteoclast activity(histology), orthodontic tooth movement velocity(CBCT, within 14 and 28 days of OTM)and relative gene expression of known inflammatory and osteoclast markers were quantified in the dental-periodontal tissue(RT–q PCR). Animals exposed to nicotine showed significantly heightened serum cotinine and IL-6 levels corresponding to those of regular European smokers. Both the extent of root resorption, osteoclast activity, orthodontic tooth movement and gene expression of inflammatory and osteoclast markers were significantly increased compared to controls with and without OTM under the influence of nicotine. We conclude that apart from increased periodontal bone loss, a progression of dental root resorption and accelerated orthodontic tooth movement are to be anticipated during orthodontic therapy, if nicotine consumption is present. Thus patients should be informed about these risks and the necessity of nicotine abstinence during treatment. | christian kirschneck michael maurer michael wolf claudia reicheneder peter proff | 2017 | International Journal of Oral Science2017，9，3： | 4 |
| 3 | Inventor collaboration over distance: A comparison of academic and corporate patents 显示文摘 | yon Proff S Dettmann A | 2013 | Scientometrics2013，94，3： | 1 |
| 4 | The application of bone graft substitutes for alveolar ridge preservation after orthodonticextractions and for augmentation of residual cleft defects显示文摘 | Proff P Bayerlein T Fanghanel J | 2006 | FoliaMorphol2006，65，1： | 1 |
| 5 | Plate osteosynthesis of the mandibuar condyle 显示文摘 | Lauer G Haim D Proff P | 2007 | Ann Anat2007，2，： | 1 |
| 6 | Occlusal contact patterns -population - based data显示文摘 | Hutzen D Proff P Gedrange T | | Ann Anat0，189，4： | 1 |
| 7 | The natural flow regime:aparadigm for river conservation and restoration显示文摘 | Proff N J Allan J D Bain M B | 1997 | Bioscience1997，47，： | 1 |
| 8 | Study on treatment of condylar process fractures of the mandible 显示文摘 | Eulert S Proff P Bokan I | 2007 | Ann Anat2007，189，4： | 1 |
| 9 | Plate osteosynthesis of the mandibu- lar condyle显示文摘 | Lauer G Haim D Proff P | 2007 | Ann Anat2007，221，： | 1 |
| 10 | Problems of supernumerary teeth,hyperdontia or dentes supernumerarii 显示文摘 | Proff P Fanghä nel J Allegrini S Jr | 2005 | Ann Anat2005，188，2： | 1 |
| 11 | The dynamics of inter-regional collaboration: an analysis of co-patenting显示文摘 | Sidonia Proff Thomas Brenner | 2014 | The Annals of Regional Science2014，，1： | 1 |
| 12 | Student evaluation of problem-based learning in a dental orthodontic currieulum-a pilot sludy 显示文摘 | Ratzmann A Wiesmann U Proff P | 2013 | GMS Z Med Ausbild2013，30，3： | 1 |
| 13 | Tourism policy in the making an Australian network study 显示文摘 | Proff C | 2006 | Annals of Tourism Research2006，33，1： | 1 |
| 14 | Evaluation of bone availability in the cleft area following secondary osteoplasty 显示文摘 | Bayerlein T Proff P Heinrich A | 2006 | J Cranio-Maxillofac Surg2006，34，2： | 1 |
| 15 | Problems of supemumerary teeth, hyperdontia or dentes supemum erarii 显示文摘 | Proff P Fanghanel J Allegrini S Jr | 2006 | Ann Anat2006，188，2： | 1 |
| 16 | Secondary palatal closure in rats in association with relative maternofetal levels of folic acid, vitamin B12, and homocysteine 显示文摘 | Weingartner J Maile S Proff P | 2007 | Annals of Anatomy2007，189，3： | 1 |
| 17 | The natural flow regime: a paradigm for river conservation and restoration显示文摘 | Proff N J Allan J D Bain M B | 1997 | Bioscience1997，47，： | 1 |
| 18 | Bacterial colonisa- tion of interior implant threads with and without sealing 显示文摘 | Proff P Steinmetz I Bayerlein T | 2006 | Folia Morphol (Warsz)2006，65，1： | 1 |
| 19 | Inventor collaboration over distance: a comparison of academic and corporate patents显示文摘 | Sidonia Proff Anja Dettmann | 2013 | Scientometrics2013，，3： | 1 |
| 20 | Measurement of tooth and implant mobility under physiological loading conditions显示文摘 | Boldt J Knapp W Proff P | | 0，，2： | 1 |
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