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| 1 | The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain——an in vitro study of human periodontal ligament fibroblasts显示文摘During orthodontic tooth movement(OTM)mechanical forces trigger pseudo-inflammatory,osteoclastogenic and remodelling processes in the periodontal ligament(PDL)that are mediated by PDL fibroblasts via the expression of various signalling molecules.Thus far,it is unknown whether these processes are mainly induced by mechanical cellular deformation(mechanotransduction)or by concomitant hypoxic conditions via the compression of periodontal blood vessels.Human primary PDL fibroblasts were randomly seeded in conventional six-well cell culture plates with O2-impermeable polystyrene membranes and in special plates with gas-permeable membranes(Lumox?,Sarstedt),enabling the experimental separation of mechanotransducive and hypoxic effects that occur concomitantly during OTM.To simulate physiological orthodontic compressive forces,PDL fibroblasts were stimulated mechanically at 2 g·cm?2 for 48 h after 24 h of pre-incubation.We quantified the cell viability by MTT assay,gene expression by quantitative real-time polymerase chain reaction(RT-qPCR)and protein expression by western blot/enzyme-linked immunosorbent assays(ELISA).In addition,PDL-fibroblast-mediated osteoclastogenesis(TRAP+cells)was measured in a 72-h coculture with RAW264.7 cells.The expression of HIF-1α,COX-2,PGE2,VEGF,COL1A2,collagen and ALPL,and the RANKL/OPG ratios at the mRNA/protein levels during PDL-fibroblast-mediated osteoclastogenesis were significantly elevated by mechanical loading irrespective of the oxygen supply,whereas hypoxic conditions had no significant additional effects.The cellular–molecular mediation of OTM by PDL fibroblasts via the expression of various signalling molecules is expected to be predominantly controlled by the application of force(mechanotransduction),whereas hypoxic effects seem to play only a minor role.In the context of OTM,the hypoxic marker HIF-1αdoes not appear to be primarily stabilized by a reduced O2 supply but is rather stabilised mechanically. | Niklas Ullrich Agnes Schroder Jonathan Jantsch Gerrit Spanier Peter Proff Christian Kirschneck | 2019 | International Journal of Oral Science2019，11，4： | 5 |
| 2 | Regular nicotine intake increased tooth movement velocity,osteoclastogenesis and orthodontically induced dental root resorptions in a rat model显示文摘Orthodontic forces have been reported to significantly increase nicotine-induced periodontal bone loss. At present, however, it is unknown, which further(side) effects can be expected during orthodontic treatment at a nicotine exposure corresponding to that of an average European smoker. 63 male Fischer344 rats were randomized in three consecutive experiments of 21 animals each(A/B/C) to 3 experimental groups(7 rats, 1/2/3):(A) cone-beam-computed tomography(CBCT);(B) histology/serology;(C) reversetranscription quantitative real-time polymerase chain reaction(RT-q PCR)/cotinine serology—(1) control;(2) orthodontic tooth movement(OTM) of the first and second upper left molar(Ni Ti closed coil spring, 0.25 N);(3) OTM with 1.89 mg·kg^(-1) per day s.c. of L(-)-nicotine. After 14 days of OTM, serum cotinine and IL-6 concentration as well as orthodontically induced inflammatory root resorption(OIIRR), osteoclast activity(histology), orthodontic tooth movement velocity(CBCT, within 14 and 28 days of OTM)and relative gene expression of known inflammatory and osteoclast markers were quantified in the dental-periodontal tissue(RT–q PCR). Animals exposed to nicotine showed significantly heightened serum cotinine and IL-6 levels corresponding to those of regular European smokers. Both the extent of root resorption, osteoclast activity, orthodontic tooth movement and gene expression of inflammatory and osteoclast markers were significantly increased compared to controls with and without OTM under the influence of nicotine. We conclude that apart from increased periodontal bone loss, a progression of dental root resorption and accelerated orthodontic tooth movement are to be anticipated during orthodontic therapy, if nicotine consumption is present. Thus patients should be informed about these risks and the necessity of nicotine abstinence during treatment. | christian kirschneck michael maurer michael wolf claudia reicheneder peter proff | 2017 | International Journal of Oral Science2017，9，3： | 4 |
| 3 | Functional 3-D analysis of patients with unilateral cleft of lip, alveplus and palate (UCLAP) Following lip repair 显示文摘 | Peter PROFF Jens WEINGARTNER Kurt ROTTNER | 2006 | J Cranio Maxiliofac Surg2006，34，2： | 1 |
| 4 | 年龄评估在正畸及全科口腔医学中的应用显示文摘在口腔医学中,如何判断年龄及牙齿和骨骼的生长发育阶段非常重要,特别是在评估牙列的发育障碍及进行正畸治疗设计时。此外,口腔医学在法医学领域也很重要,可以根据牙列情况确定年龄。除了人体测量学和形态学方法之外,还有许多生物化学,组织学,放射学和无放射的成像方法可以用来确定一个人或一个病人的实际年龄、牙龄和骨龄。本文旨在综述目前口腔医学中用于年龄评估的可行方法,既可用于法医学领域又可用于治疗诊断目的,并基于目前实验结果批判性评估不同方法的适应证及其价值。 | Christian Kirschneck Peter Proff 付肖依(译) 聂琼(审) | 2019 | 中国口腔医学继续教育杂志2019，22，5： | 1 |
| 5 | Associ ation of dentoskeletal morphology withincisor inclination in an gle class II patients: aretrospective cephalometric study 显示文摘 | Christian Kirschneck Piero Romer Peter Proff | 2013 | Head & Face Medicine2013，9，： | 1 |
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