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| 1 | Primary cilia support cartilage regeneration after injury显示文摘In growing children,growth plate cartilage has limited self-repair ability upon fracture injury always leading to limb growth arrest.Interestingly,one type of fracture injuries within the growth plate achieve amazing self-healing,however,the mechanism is unclear.Using this type of fracture mouse model,we discovered the activation of Hedgehog(Hh)signaling in the injured growth plate,which could activate chondrocytes in growth plate and promote cartilage repair. | Dike Tao Lei Zhang Yunpeng Ding Na Tang Xiaoqiao Xu Gongchen Li Pingping Niu Rui Yue Xiaogang Wang Yidong Shen Yao Sun | 2023 | International Journal of Oral Science2023，15，2： | 1 |
| 2 | Overexpression of UDP-glucose pyrophosphorylase gene could increase cellulose content in Jute ( Corchorus capsularis L.)显示文摘 | Gaoyang Zhang Jianmin Qi Jiantang Xu Xiaoping Niu Yujia Zhang Aifen Tao Liwu Zhang Pingping Fang LiHui Lin | 2013 | Biochemical and Biophysical Research Communications . 2013 (3-4)2013，，3： | 1 |
| 3 | Cell-free DNA derived from cancer cells facilitates tumor malignancy through Toll-like receptor 9 signaling-triggered interleukin-8 secretion in colorectal cancer显示文摘 | Zhengchuan Niu Wentao Tang Tianyu Liu Pingping Xu Dexiang Zhu Meiling Ji Wenbai Huang Li Ren Ye Wei Jianmin Xu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，10： | 1 |
| 4 | Determination of sterols in tobacco leaves by gas chromatography-tandem mass spectrometry显示文摘The purpose of the study was to establish an analytical method to simultaneously determine multiple sterols in tobacco leaves rapidly and accurately.In this gas chromatography-triple quadruple tandem mass spectrometry(GC-MS/MS)based method,various conditions for sterols extraction were assessed and the instrumental operation parameters were optimized with 5α-cholane as an internal standard.Using this method,eight plant sterols,namely lanosterol,campesterol,chenodeoxycholic acid,cholesterol,β-sitosterol,stigmasterol,dihydrotachysterol and ergosterol,were separated in less than 30 min.The linear correlation coefficients were over 0.998 9,the low detection limits were in the range of 0.010 3-0.141 4 μg/g.The recoveries were from 87.30% to 115.60%,with low standard deviations.In conclusion,this method demonstrates good repeatability,accuracy,and high sensitivity,and is best suited for rapid analysis of multiple sterols in tobacco leaves. | CHEN Qiansi LIU Pingping ZHAI Niu ZHENG Qingxia XU Guoyun WANG Chen FAN Kai JIN Lifeng ZHANG Hui ZHOU Huina | 2018 | 烟草科技2018，51，A01： | 0 |
| 5 | Hepatic COX1 loss leads to impaired autophagic flux and exacerbates nonalcoholic steatohepatitis显示文摘The mechanisms underlying autophagic defects in nonalcoholic steatohepatitis(NASH)remain largely unknown.We aimed to elucidate the roles of hepatic cyclooxygenase 1(COX1)in autophagy and the pathogenesis of diet-induced steatohepatitis in mice.Human nonalcoholic fatty liver disease(NAFLD)liver samples were used to examine the protein expression of COX1 and the level of autophagy.Cox1^(Δhepa)mice and their wildtype littermates were generated and fed with 3 different NASH models.We found that hepatic COX1 expression was increased in patients with NASH and diet induced NASH mice models accompanied by impaired autophagy.COX1 was required for basal autophagy in hepatocytes and liver specific COX1 deletion exacerbated steatohepatitis by inhibiting autophagy.Mechanistically,COX1 directly interacted with WD repeat domain,phosphoinositide interacting 2(WIPI2),which was crucial for autophagosome maturation.Adeno-associated virus(AAV)-mediated rescue of WIPI2 reversed the impaired autophagic flux and improved NASH phenotypes in Cox1^(Δhepa)mice,indicating that COX1 deletion-mediated steatohepatitis was partially dependent on WIPI2-mediated autophagy.In conclusion,we demonstrated a novel role of COX1 in hepatic autophagy that protected against NASH by interacting with WIPI2.Targeting the COX1 WIPI2 axis may be a novel therapeutic strategy for NASH. | Qian Yu Chang Li Qinghui Niu Jigang Wang Zhaodi Che Ke Lei He Ren Boyi Ma Yixing Ren Pingping Luo Zhuming Fan Huan Zhang Zhaohui Liu George L.Tipoe Jia Xiao | 2023 | Acta Pharmaceutica Sinica B2023，13，6： | 0 |
| 6 | Higher Education Expansion, Selections Based on Non-Cognitive Abilities and Gender Differences: The Case of China显示文摘We assess how implicit selections based on non-cognitive abilities may have changed in the context of the great higher education expansion in China,applying the classic supply-demand framework and utilizing the Big Five personality model.Using the Chinese General Social Survey(CGSS)data from 2012,difference-in-difference(DID),difference-in-difference-in-difference(DDD)and descriptive analyses establish the following three major findings.First,from the generation entering college before the expansion-post-70s-to the generation entering college after the expansion-post-80s and post-90s-implicit selections in higher education based on personality have weakened with the increased supply of higher education opportunities.Second,selections have significantly weakened in terms of the openness dimension of personality,yet there is some evidence that selections have strengthened in terms of conscientiousness.This reflects that open-mindedness has become a relatively higher supply trait and conscientiousness has become a relatively lower supply trait among members of the post-90s generation.Third,selections have weakened only on the openness dimension for males,but on multiple dimensions beginning with agreeableness for females,reflecting a greatly increased supply of higher education opportunities for females with this dominant trait.The finding on strengthened selections based on conscientiousness has important implications for what and how to educate today’s college students. | YANG Ying NIU Xinchun YANG Fei HU Pingping | 2020 | Frontiers of Education in China2020，15，2： | 0 |
| 7 | An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease显示文摘Rice ARGONAUTE2(OsAGO2)is a core component of the rice RNA-induced silencing complex(RISC),which is repressed by Magnaporthe oryzae(M.oryzae)infection.Whether and how OsAGO2-mediated gene silencing plays a role in rice blast resistance and which sRNAs participate in this process are unknown.Our results indicate that OsAGO2 is a key immune player that manipulates rice defense responses against blast disease.OsAGO2 associates with the 24-nt miR1875 and binds to the promoter region of HEXOKINASE1(OsHXK1),which causes DNA methylation and leads to gene silencing.Our multiple genetic evidence showed that,without M.oryzae infection,OsAGO2/miR1875 RISC promoted OsHXK1 promoter DNA methylation and OsHXK1 silencing;after M.oryzae infection,the reduced OsAGO2/miR1875 led to a relatively activated OsHXK1 expression.OsHXK1 acts as a positive regulator of blast disease resistance that OsHXK1-OE rice exhibited enhanced resistance,whereas Cas9-Oshxk1 rice showed reduced resistance against M.oryzae infection.OsHXK1 may function through its sugar sensor activity as glucose induced defense-related gene expression and reactive oxygen species(ROS)accumulation in Nipponbare and OsHXK1-OE but not in Cas9-Oshxk1 rice.OsAGO2 itself is delicately regulated by OsPRMT5,which senses M.oryzae infection and attenuates OsAGO2-mediated gene silencing through OsAGO2 arginine methylation.Our study reveals an OsPRMT5-OsAGO2/miR1875-OsHXK1 regulatory module that fine tunes the rice defense response to blast disease. | Cong Sheng Xuan Li Shengge Xia Yimai Zhang Ze Yu Cheng Tang Le Xu Zhaoyun Wang Xin Zhang Tong Zhou Pingping Nie Ayesha Baig Dongdong Niu Hongwei Zhao | 2023 | Journal of Integrative Plant Biology2023，65，4： | 0 |
| 8 | Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell显示文摘Dear Editors,The coronavirus disease 2019(COVID-19),caused by SARS-CoV-2,broke out in early December 2019 has escalated into a global pandemic(Lai et al.2020).Till the May 20 th 2020,more than 4,700,000 people were infected and the number is still increasing especially in Europe,North America and Asia(http://gffzze7c39cdc9e894ca6sc0pqpvvnpo0c65ow.ffgz.tsg.suse.edu.cn/). | Pingping Yao Yachun Zhang Yisheng Sun Yulin Gu Fang Xu Bo Su Chen Chen Hangjing Lu Dehui Wang Zhangnv Yang Biao Niu Jiancai Chen Lixia Xie Lei Chen Yajing Zhang Hui Wang Yuying Zhao Yue Guo Juncheng Ruan Zhiyong Zhu Zhenfang Fu Dayong Tian Qi An Jianmin Jiang Hanping Zhu | 2020 | Virologica Sinica2020，35，3： | 0 |
| 9 | Generation of functional oligopeptides that promote osteogenesis based on unsupervised deep learning of protein IDRs显示文摘Deep learning(DL)is currently revolutionizing peptide drug development due to both computational advances and the substantial recent expansion of digitized biological data.However,progress in oligopeptide drug development has been limited,likely due to the lack of suitable datasets and difficulty in identifying informative features to use as inputs for DL models.Here,we utilized an unsupervised deep learning model to learn a semantic pattern based on the intrinsically disordered regions of~171 known osteogenic proteins.Subsequently,oligopeptides were generated from this semantic pattern based on Monte Carlo simulation,followed by in vivo functional characterization.A five amino acid oligopeptide(AIB5P)had strong bone-formation-promoting effects,as determined in multiple mouse models(e.g.,osteoporosis,fracture,and osseointegration of implants).Mechanistically,we showed that AIB5P promotes osteogenesis by binding to the integrinα5 subunit and thereby activating FAK signaling.In summary,we successfully established an oligopeptide discovery strategy based on a DL model and demonstrated its utility from cytological screening to animal experimental verification. | Mingxiang Cai Baichuan Xiao Fujun Jin Xiaopeng Xu Yuwei Hua Junhui Li Pingping Niu Meijing Liu Jiaqi Wu Rui Yue Yong Zhang Zuolin Wang Yongbiao Zhang Xiaogang Wang Yao Sun | 2022 | Bone Research2022，10，2： | 0 |
| 10 | Separation and Identification of Stage-specific Proteins in Pistillate Flowers of Mulberry显示文摘In order to excavate the function genes of stage-specific proteins in the development process of mulberry pistillate flowers,using fruit mulberry cultivar‘Da 10’as experiment material,two-dimensional electrophoresis and mass-spectrometric technology were used to investigate specifically expressed proteins of mulberry pistillate flowers in different flowering periods.The results showed that 471±4,450±15 and 446±14 protein spots were determined in mulberry pistillate flowers at full-bloom stage,initial flowering stage and terminal flowering stage respectively,including nine protein spots with characteristics of stage-specific expression and clear electrophoretic bands..By mass spectrometry analysis,database retrieval and bioinformatics analysis,five components were successfully identified as lactoylglutathione lyase-like protein,perchloric acid soluble translation inhibitor protein,ubiquitin-conjugating enzyme 1 like protein,putative ethylene response protein and 3-hydroxyisobutyrate dehydrogenase,which were involved in stress resistance reaction,protein catabolism,signal transduction,glycometabolism and other biological processes in plants,which indicated that these proteins might play an important biological function in the normal development and pollination fertilization of mulberry pistillate flowers. | Ruihe NIU Yuanyuan CHEN Pingping ZHANG Wei QI Biping ZHENG Jianzhong TAN | 2013 | Agricultural Biotechnology2013，2，6： | 0 |
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