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| 1 | Eocene Basins on the SE Tibetan Plateau: Markers of Minor Offset along the Xuelongshan–Diancangshan–Ailaoshan Structural System显示文摘The offset of geological bodies provides robust evidence of displacement along a fault or ductile shear zone. The amount of displacement along the Xuelongshan–Diancangshan–Ailaoshan structural system, southeastern Tibetan Plateau, is uncertain because of the lack of offset geological markers. This NNW–SSE-trending system is developed in three isolated metamorphic complexes and interjacent nonmetamorphosed rocks. They are expected to record similar post-Eocene strain, although their structural patterns should be distinct. Geological mapping in the area between the Xuelongshan and Diancangshan metamorphic complexes has revealed a small Eocene basin, the Madeng Basin, located to the west of the structural system. The sedimentary and volcanic successions of the Madeng Basin are comparable to those of the Jianchuan Basin, which is located to the east of the structural system. Zircon U–Pb geochronological and bulk geochemical data demonstrate that the volcanic rocks of both basins formed during 37–34 Ma and share the same geochemical features. These data suggest that the Madeng and Jianchuan basins previously constituted a single basin, with the distribution of high-K volcanic rocks in the basins defining an ENE–WSW-trending volcanic belt that shows a limited dextral offset of ≤20 km across the Xuelongshan–Diancangshan–Ailaoshan structural system. Therefore, the northern segment of the structural system records no evidence of large-scale lateral movement/displacement. The results suggest that the Indochina block, which is bounded by the Xuelongshan–Diancangshan–Ailaoshan structural system to the east and the Sagaing Fault to the west, has not extruded southward as a whole but rather has been deformed by pervasive crustal shortening. | LIAO Cheng YANG Tiannan XUE Chuandong LIANG Mingjuan XIN Di XIANG Kun JIANG Lili SHI Pengliang ZHU Wenbin WAN Liangchun TANG Jing YU Jing WU Pinglei | 2020 | Acta Geologica Sinica(English Edition)2020，94，4： | 5 |
| 2 | Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display library显示文摘AIM: To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naive scFv phage display library.METHODS: A large human naive scFv phage library was used to search for the specific targets by biopanning with the hepatocellular carcinoma cell line HepG2 for the positiveselecting and the normal liver cell line L02 for the counterselecting. After three rounds of biopanning, individual scFv phages binding selectively to HepG2 cells were picked out. PCR was carried out for identification of the clones containing scFv gene sequence. The specific scFv phages were selected by ELISA and flow cytometry. DNA sequences of positive clones were analyzed by using Applied Biosystem Automated DNA sequencers 3 730. The expression proteins of the specific scFv antibody fragments in E. coli HB2151were purified by the affinity chromatography and detected by SDS-PAGE, Western blot and ELISA. The biological effect of the soluble antibody fragments on the HepG2 cells was investigated by observing the cell proliferation.RESULTS: Two different positive clones were obtained and the functional variable sequences were identified.Their DNA sequences of the scFv antibody fragments were submitted to GenBank (accession nos: AY686498 and AY686499). The soluble scFv antibody fragments were successfully expressed in E. coli HB2151. The relative molecular mass of the expression products was about 36 ku,according to its predicted Mr value. The two soluble scFv antibody fragments also had specific binding activity and obvious growth inhibition properties to HepG2 cells.CONCLUSION: The phage library biopanning permits identification of specific antibody fragments for hepatocellular carcinoma and affords experiment evidence for its immunotherapy study. | BingYu MingNi Wen-HanLi PingLei WeiXing Dai-WenXiao YuHuang Zhen-JieTang Hui-FenZhu Guan-XinShen | 2005 | World Journal of Gastroenterology2005，11，26： | 2 |
| 3 | Construction and Co-expression of Bicistronic Plasmid Encoding Human WEE1 and Stem Cell Factor显示文摘To protect the hematopoietic stem cells (HSCs) from apoptosis induced by chemotherapy and promote HSC proliferation, bi-functional gene delivery systems are increasingly investigated in gene therapy.In the present study, we constructed a bicistronic vector, pWISG, expressing the anti-apoptotic protein human WEE1 (WEE1Hu) and the fusion protein of the proliferation-stimulating stem cell factor (SCF) and enhanced green fluorescent protein (EGFP) separately with internal ribosome entry site (IRES). We first examined the expression and location of WEE1Hu in Chinese hamster ovary (CHO) cells and showed that WEE1Hu was located in the nucleus, which was confirmed by immunohistochemistry and Western blot. Wedetermined the expression and receptor-binding ability of the SCF-EGFP fusion protein on CD34^+ cells,which were proved by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry,respectively. Furthermore, inhibition of cisplatin-induced apoptosis was observed in CD34^+ cells transfected with pWISG, which implies that protection for CD34^+ cells was achieved via WEE1Hu and SCF-EGFP. Our study suggests that the introduction of two functional genes via bicistronic vector is more powerful and efficient than single gene therapy. | PingLEI Wen-HanLI Wen-JunLIAO BingYU Hui-FenZHU Jing-FangSHAO Guan-XinSHEN | 2005 | Acta Biochimica et Biophysica Sinica2005，37，2： | 2 |
| 4 | A context-aware scheme for privacy-preserving location-based services显示文摘 | Aniket Pingley Wei Yu Nan Zhang Xinwen Fu Wei Zhao | 2012 | Computer Networks2012，，11： | 1 |
| 5 | Primary carcinoma of the vagina:Tata Memorial Hospital experience显示文摘 | Pingley S Shrivastava SK Sarin R | 2000 | Int J Radiat Oncol Biol Phys2000，46，1： | 1 |
| 6 | Gray matter atrophy in Parkinson' s disease with dementia: evidence from meta-analysis of voxel-based morphometry studies 显示文摘 | PAN Pinglei SHI Haicun ZHONG J G | 2013 | Neu- rol Sci2013，34，5： | 1 |
| 7 | The Inhibitory Effects of Mouse ICOS-Ig Gene-Modified Mouse Dendritic Cells on T Cells显示文摘The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present. Although these strategies have reportedly reduced graft rejection, there has been a reciprocal increase in more severe immunosuppression and lethal infections, as well as severe side effects. Blockade of costimulatory T cell response has been proved as one of useful strategies to reduce graft rejection. Furthermore,it has been shown that infusion of dendritic cells (DCs) with a potent negative regulatory ability for T cells could prolong allograft survival. In this study mouse DCs (mDCs) were transfected with the recombinant plasmid pcDNA3.0 containing mouse inducible costimulator-Ig (mICOS-Ig) cDNA by electroporation. The transient expression of mICOS-Ig in mDC could be detected by ELISA and SDS-PAGE. Mouse ICOS-Ig fusion protein expressed in mDC and mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in mixed lymphocyte culture (MLC) in vitro. Furthermore, mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in recipient mice. These results suggested that mICOS-Ig gene-modified mDC exerted inhibitory effects on T cells, and might be suitable for treatment or prevention of graft rejection and immunopathologicdiseases. | GuohuaWang LijuanZhu PingHu HuifenZhu PingLei WenjunLiao BingYu FeiliGong GuanxinShen | 2004 | Cellular & Molecular Immunology2004，1，2： | 1 |
| 8 | A context-aware scheme for privacy-preserving location-based services显示文摘 | Aniket Pingley Wei Yu Nan Zhang Xinwen Fu Wei Zhao | 2012 | Computer Networks2012，，11： | 1 |
| 9 | A context-aware scheme for privacy-preserving location-based services显示文摘 | PINGLEY A YU W ZHANG N | 2012 | Computer Networks2012，56，11： | 1 |
| 10 | The digital marauder's map: A WiFi forensic positioning tool显示文摘 | FU X ZHANG N PINGLEY A | 2012 | IEEE Transactions on Mobile Computing2012，11，3： | 1 |
| 11 | A Context-aware Scheme for Privacy-preserving Location-based Services显示文摘 | Pingley A Yu Wei Zhang Nan | 2012 | Computer Networks2012，56，11： | 1 |
| 12 | Construction and Co-expression of Bicistronic Plasmid Encoding Human WEE1 and Stem Cell Factor显示文摘 | PingLEI Wen-HanLI Wen-JunLIAO BingYU Hui-FenZHU Jing-FangSHAO Guan-XinSHEN | 2006 | 中国生物学文摘2006，20，6： | 0 |
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