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| 1 | Review of Key Problems Related to Integrated Energy Distribution Systems显示文摘Integrated energy distribution system(IEDS)is one of the integrated energy and power system forms,which involves electricity/gas/cold/heat and other various energy forms.The energy coupling relationship is close and complex.IEDS is the focus of regional energy internet research and development at home and abroad.Compared with the traditional power distribution system,IEDS through the multi-energy coupling link comprehensive utilization,effectively improve the distribution system economy,safety,reliability,flexibility and toughness,but also to ease the regional energy system environmental pressure.IEDS is an important direction for the future development of energy systems,and its related research and practice on China’s energy system development also has important practical and strategic significance.This paper summarizes the related researches of the IEDS and explores the energy operation characteristics and coupling mechanisms.What’s more,the integrated model of IEDS is summarized.On these bases,this paper discusses and prospects some key issues such as joint planning,optimization control and security analysis,state estimation and situational awareness and generalized demand side management. | Dan Wang Liu Liu Hongjie Jia Weiliang Wang Yunqiang Zhi Zhengji Meng Bingyu Zhou | 2018 | CSEE Journal of Power and Energy Systems2018，4，2： | 43 |
| 2 | Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment显示文摘A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land. | Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan | 2007 | Chinese Science Bulletin2007，52，2： | 10 |
| 3 | Genetic Analysis of Two Weak Dormancy Mutants Derived from Strong Seed Dormancy Wild Type Rice N22 (Oryza sativa)显示文摘Two weak dormancy mutants,designated Q4359 and Q4646,were obtained from the rice cultivar N22 after treatment with 400 Gy 60Co gamma-radiation.Compared to the N22 cultivar,the dormancy of the mutant seeds was more readily broken when exposed to a period of room temperature storage.The mutants also showed a reduced level of sensitivity to abscisic acid compared to the N22 cultivar,although Q4359 was more insensitive than Q4646.A genetic analysis indicated that in both mutants,the reduced dormancy trait was caused by a single recessive allele of a nuclear gene,but that the mutated locus was different in each case.The results of quantitative trait locus(QTL)mapping,based on the F2 population from Q4359 x Nanjing35,suggested that Q4359 lacks the QTL qSdn-1 and carries a novel allele at QTL qSdn-9, while a similar analysis of the Q4646 x Nanjing35 F2 population suggested that Q4646 lacks QTL qSdn-5, both qSdn-1 and qSdn-5 are major effect seed dormancy QTL in N22.Therefore,these two mutants were helpful to understand the mechanism of seed dormancy in N22. | Bingyue Lu KunXie Chunyan Yang Long Zhang Tao Wu Xi Liu Ling Jiang Jianmin Wan | 2011 | Journal of Integrative Plant Biology2011，53，5： | 7 |
| 4 | Research on analysis method for temperature control information of high arch dam construction显示文摘Temperature control,which is directly responsible for the project quality and progress,plays an important role in high arch dam construction.How to discover the rules from a large amount of temperature control information collected in order to guide the adjustment of temperature control measures to prevent cracks on site is the key scientific problem.In this paper,a mathematic logical model was built firstly by means of a coupling analysis of temperature control system decomposition and coordination for high arch dam.Then,an analysis method for temperature control information was presented based on data mining technology.Furthermore,the data warehouse of temperature control was designed,and the artificial neural network forecasting model for the highest temperature of concrete was also developed.Finally,these methods were applied to a practical project. The result showed that the efficiency and precision of temperature control was improved,and rationality and scientificity of management and decision-making were strengthened.All of these researches provided an advanced analysis method for temperature control in the high arch dam construction process. | ZHONG DengHua,ZHAO ChenSheng & REN BingYu Department of Hydraulic and Hydroelectric Engineering,School of Civil Engineering,Tianjin University,Tianjin 300072,China | 2011 | Science China(Technological Sciences)2011，54，S1： | 6 |
| 5 | Epigenetic modification in histone deacetylase deletion strain of Calcarisporium arbuscula leads to diverse diterpenoids显示文摘Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells. | Jian Bai Rong Mu Man Dou Daojiang Yan Bingyu Liu Qian Wei Jun Wan Yi Tang Youcai Hu | 2018 | Acta Pharmaceutica Sinica B2018，8，4： | 5 |
| 6 | Smoother and Bayesian filter based semi-codeless tracking of dual-frequency GPS signals显示文摘To precisely determine the integrated orbit of the Chinese manned spacecraft mission,a smoother and Bayesian filter based technique for optimum semi-codeless tracking of the P(Y) code on dual-frequency GPS signals has been advanced. This signal processing technique has been proven effective and robust for affording access to dual-frequency GPS signals. This paper introduces the signal dynamics and measurement models, describes the W·D bit estimation method, and corrects the mistakes of direct estimation of W bit in current semi-codeless tracking. Median filter is chosen as a smoother to find the best measurements at the current time among the history and current information. The Bayesian filter is used to track the L2 P(Y) code phase and L2 carrier phase recursively. | LIAO Bingyu YUAN Hong LIN Baojun | 2006 | Science in China(Series F)2006，49，4： | 5 |
| 7 | Diurnal variation of nitrated polycyclic aromatic hydrocarbons in PM_(10) at a roadside site in Xiamen,China显示文摘Intensive daytime and nighttime sampling was carried out from 23 Oct to 31 Dec 2008 to investigate the occurrence of nitrated polycyclic aromatic hydrocarbons(NPAHs) in PM 10 at a roadside site in Xiamen,China.At the same time,six PM 10 samples were collected from a nearby roadway tunnel for comparison.Six NPAHs,namely 9-nitroanthracene,2-and 3-nitrofluoranthene,1nitropyrene,7-nitrobenz[a]anthracene,and 6-nitrobenzo[a]pyrene,were identified and quantified using GC/MS in negative ion chemical ionization mode.The average total concentration of six NPAHs(∑ NPAHs) in the cold season(26 Nov-31 Dec) was 2.3(daytime) and 9.9(nighttime) times higher than those in the warm season.Significant statistical difference(p < 0.01,2-tailed) of ∑ NPAHs between daytime and nighttime was found during both the warm and cold seasons.NPAHs were significantly positively correlated with their parent PAHs and nitrogen dioxide but negatively correlated with ambient temperature.The ratio of 2 + 3-nitrofluoranthene to 1nitropyrene exhibited a similar diurnal pattern as ∑ NPAHs and was generally greater than 5,indicating the importance of secondary atmospheric formation.The diurnal variations of NPAHs were all influenced by the diurnal variations of PAHs,nitrogen dioxide,sunlight,and temperature.The daily inhalable exposure to the six NPAHs in the tunnel was much higher than the roadside values in the warm season but only slightly higher than those in the cold season. | Shuiping Wu Bingyu Yang Xinhong Wang Huasheng Hong Chungshin Yuan | 2012 | Journal of Environmental Sciences2012，24，10： | 5 |
| 8 | Study on coupled 3D seepage and stress fields of the complex channel project显示文摘Seepage is a vital reason that may bring many adverse consequences such as subsidence,inclination and fracture to the channel,and is harmful to the safety and stability of the channel.Thus,a 3D visualization model is established for the engineering geological information of the research channel section by using NURBS-TIN-BRep hybrid data structure.Coupled with the VOF(volume of fluid)method,the N-S(Navier-Stokes)equations are applied to seepage simulation of the research channel section.Then the stability of the channel is studied coupled with the seepage simulation results,to comprehensively analyze the stress and displacement conditions of the channel under the impact of different factors such as seepage and underground goafs.The results of this study illustrate that the channel seepage has great influence on its stability,especially on the displacement field:it will lead to a significant sedimentation to the foundation.Therefore,during the practical construction,it is suggested that the certain part of the channel should be reinforced and effective seepage control measures should be taken. | ZHONG DengHua ZHANG XiaoXin AO XueFei WANG XiaoLing TONG DaWei REN BingYu | 2013 | Science China(Technological Sciences)2013，56，8： | 5 |
| 9 | Artemisinin ameliorated proteinuria in rats with adriamycin-induced nephropathy through regulating nephrin and podocin expressions显示文摘OBJECTIVE:To investigate the effects of artemisinin against proteinuria and glomerular filtration barrier damage in rats with adriamycin-induced nephropathy,and the potential mechanism underpinned the action.METHODS:Forty adriamycin rats were randomly divided into two groups with the ratio of 1︰3;the small-number group served as control group(n=10),and the rats in the large-number group were treated with adriamycin to induce nephropathy;then they were further randomly assigned into 3subgroups:benazepril group(n=10),artemisiningroup(n=10),and adriamycin group(n=10).The benazepril group and artemisinin group were treated with benazepril suspl(5.0 mg/kg daily)and artemisinin suspl(150 mg/kg daily)respectively after being modeled;those in the control group and adriamycin group were intragastrically administered an equivalent volume of distilled water every day.The treatment after model establishment lasted for a total of 4 weeks.The 24 h uric protein,blood biochemicals,renal pathological changes,renal ultrastrutural changes,Nephrin and Podocin proteins and gene expressions were measured by Coomassie brilliant blue assay,completely automatic biochemical analyzer,light microscope,electron microscopy,Western blot and reverse transcription polymerase chain reaction,respectively.RESULTS:The rats in adriamycin group showed a significant increase in 24 h uric protein excretion,serum total cholesterol(TC),triglyceride(TG),blood urea nitrogen(BUN),serum creatinine(Scr)and decrease in albumin(Alb)(P<0.05 or P<0.01).Compared with adriamycin group,artemisinin could reduce uric protein excretion,decrease the serum TC,TG elevation,increase the serum Alb level,up-regulate the expressions of Nephrin and Podocin(P<0.05 or P<0.01),but no statistical significance effects on the levels of BUN,Scr in artemisinin group(P>0.05).The renal pathological and ultrastrutural observation indicate that artemisinin could attenuate the severity of foot process effacement and fusion in the nephropathic rats.CONCLUSION:Artemisinin might have an effect on the nephropathy in rats caused by adriamycin,which may be at least partly correlated with attenu-ation of the severity of foot process effacement and fusion,up-regulation of the expressions of Nephrin and Podocin in the glomeruli in the rats. | Xili Wu Peng An Bingyu Ye Xingmin Shi Huimin Dang Rongguo Fu Chenglin Qiao | 2014 | Journal of Traditional Chinese Medicine2014，34，1： | 5 |
| 10 | Dynamic expression of the LAP family of genes during early development of Xenopus tropicalis显示文摘The leucine-rich repeats and PDZ (LAP) family of genes are crucial for the maintenance of cell polarity as well as for epithelial homeostasis and tumor suppression in both vertebrates and invertebrates.Four members of this gene family are known:densin,erbin,scribble and lano.Here,we identified the four members of the LAP gene family in Xenopus tropicalis and studied their expression patterns during embryonic development.The Xenopus LAP proteins show a conserved domain structure that is similar to their homologs in other vertebrates.In Xenopus embryos,these genes were detected in animal cap cells at the early gastrula stage.At later stages of development,they were widely expressed in epithelial tissues that are highly polar in nature,including the neural epithelia,optic and otic vesicles,and in the pronephros.These data suggest that the roles of the Xenopus LAP genes in the control of cell polarity and morphogenesis are conserved during early development.Erbin and lano show similar expression patterns in the developing head,suggesting potential functional interactions between the two molecules in vivo. | YANG QiuTan LV XiaoYan KONG QingHua LI ChaoCui ZHOU Qin MAO BingYu | 2011 | Science China(Life Sciences)2011，54，10： | 3 |
| 11 | Extraction Process Optimization and Moisturizing Performance of Fucoidan from Sargassum fusiforme显示文摘[Objectives] The research aimed to optimize extraction process of fucoidan from Sargassum fusiforme( FSF) and study its moisturizing performance. [Methods]Extracting condition of FSF by cellulase hydrolysis-ultrasonic assisted extraction method was optimized. The influences of solvent p H,enzyme dosage,extraction temperature,cellulose hydrolysis time,ultrasonication time,and the ratio of material to liquid on FSF were investigated by single factor and orthogonal experiments. [Results] The optimum extraction conditions were as followings:p H,4. 5; enzyme dosage,1%; extraction temperature,40℃; cellulose hydrolysis time,2 h; ultrasonic time,15 min; and the ratio of material to liquid,1∶ 10( g∶ m L). Under the optimal condition,the extraction yield of FSF was 8. 50%,RSD = 2. 74%. The short-time hygroscopicity( within 8 h) of crude extract of fucoidan from S. fusiforme( CEFSF) was better than glycerin,butanediol,and sodium alginate,and the moisture retention capacity of 1% CEFSF aqueous solution was better than 1% butanediol or 1% sodium alginate,and was equal to 5% glycerin under relative humidity of 43% and 81%. The determination results of skin moisture content and transepidermal water loss rate( TEWL)showed that: 5% CEFSF solution had good moisturizing effect. [Conclusions]The research could provide certain reference for deep development of S. fusiforme. | Bingyue LIU Xue LIU Fengjuan WU Guangqun CAO Chen ZHANG Jie YANG | 2018 | Medicinal Plant2018，9，3： | 3 |
| 12 | Traditional Chinese medicines and their active ingredients sensitize cancer cells to TRAIL-induced apoptosis显示文摘The rapidly developing resistance of cancers to chemotherapy agents and the severe cytotoxicity of such agents to normal cells are major stumbling blocks in current cancer treatments.Most current chemotherapy agents have significant cytotoxicity,which leads to devastating adverse effects and results in a substandard quality of life,including increased daily morbidity and premature mortality.The death receptor of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)can sidestep p53-dependent pathways to induce tumor cell apoptosis without damaging most normal cells.However,various cancer cells can develop resistance to TRAIL-induced apoptosis via different pathways.Therefore,it is critical to find an efficient TRAIL sensitizer to reverse the resistance of tumor cells to TRAIL,and to reinforce TRAIL’s ability to induce tumor cell apoptosis.In recent years,traditional Chinese medicines and their active ingredients have shown great potential to trigger apoptotic cell death in TRAIL-resistant cancer cell lines.This review aims to collate information about Chinese medicines that can effectively reverse the resistance of tumor cells to TRAIL and enhance TRAIL’s ability to induce apoptosis.We explore the therapeutic potential of TRAIL and provide new ideas for the development of TRAIL therapy and the generation of new anticancer drugs for human cancer treatment.This study involved an extensive review of studies obtained from literature searches of electronic databases such as Google Scholar and PubMed.'TRAIL sensitize'and'Chinese medicine'were the search keywords.We then isolated newly published studies on the mechanisms of TRAIL-induced apoptosis.The name of each plant was validated using certified databases such as The Plant List.This study indicates that TRAIL can be combined with different Chinese medicine components through intrinsic or extrinsic pathways to promote cancer cell apoptosis.It also demonstrates that the active ingredients of traditional Chinese medicines enhance the sensitivity of cancer cells to TRAIL-mediated apoptosis.This provides useful information regarding traditional Chinese medicine treatment,the development of TRAIL-based therapies,and the treatment of cancer. | Bingyu SUN Yongqiang LIU Danhua HE Jinke LI Jiawei WANG Wulin WENS Ming HONG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，3： | 3 |
| 13 | Effect of rare earth on the performance of Ru/MgAl-LDO catalysts for ammonia synthesis显示文摘A series of MgAl-layered double oxides(LDO) doped with different rare-earth elements(Y, La, and Ce)were synthesized by the calcination of Mg-Al layered double hydroxides, and Ru, which were used to prepare ammonia synthesis catalysts. The as-obtained oxides and catalysts were characterized by XRD,TEM, TPD, TPR and XPS to understand their catalytic performances in ammonia synthesis. The H_2-TPR and HRTEM studies reveal that Ru/Y-LDO catalyst possesses more active Ru metal and small particle size.The XPS demonstrates that the electronic interaction between Y and Ru metals is stronger, which can be tentatively explained by most of Y inserted into the hydrotalcites structure. CO_2-TPD demonstrates that Ru/Y-LDO catalyst shows stronger basic site densities than catalysts doped with Ce and La. Higher activity of the Ru/Y-LDO catalyst can be attributed to smaller particle size, more active metal(Ru) and strong Ru-support interaction. | Jun Ni Baoqiang Jing Jianxin Lin Bingyu Lin Ziqi Zhao Lilong Jiang | 2018 | Journal of Rare Earths2018，36，2： | 3 |
| 14 | Experimental Study on the Protection of Agrimony Extracts from Different Extracting Methods against Cerebral Ischemia-Reperfusion Injury显示文摘Objective To study the protective effect of agrimony extracts from different extracting methods on cerebral ischemia-reperfusion injury in rats, in order to optimize the extraction scheme of agrimony.Methods Male rats were randomly assigned into seven groups: 1. Sham-operated group, 2. Untreated MCAO group (MCAO), 3. Petroleum ether extract of Agrimonia pilosa treated MCAO group (PEA), 4. Ethyl acetate extract of Agrimonia pilosa treated MCAO group (EAEA), 5. Ethanol extract of Agrimonia pilosa treated MCAO group (EEA), 6. Water extract of Agrimonia pilosa treated MCAO group (WEA), 7. Nimodipine treated MCAO group (NP). Intragastrical drug administration (i.g) was performed at 0 and 6 hours after MCAO.Neurological function tests were performed after reperfusion for 24 hours, then the brain was removed for the evaluations of the cerebral infarction volume (percentage of total brain volume) by immunohistochemistry,histological changes (hematoxylin-eosin staining), Na+/K+-ATPase, Ca2+-ATPase (modified method of Svoboda and Mosinger), mRNA expression of Tumor suppressor gene (P53) and hot shock protein (HSP70)(quantitative real-time PCR).Results The neurological function of MCAO group had significantly higher scores than the sham group (P<0.01). The WEA group showed a significantly lower neurological score than the MCAO group (P<0.05),indicating the protective effect of WEA on neurological deficits. The mean infarction volumes of WEA (13.5±6.6%, F=4.75, P<0.01), EEA (19.90±6.90%, F=5.23, P<0.01), PEA (20.40±5.30%, F=4.68,P<0.01) and EAEA (22.50±10.50%, F=6.25, P<0.05) group were all significantly smaller than that of MCAO group (29.40±6.50%). HE staining demonstrated that, compared to the treated groups, the infarcted cerebral tissue of MCAO group had more swelling neural cells, lighter stained nucleus, fewer and irregularly distributed neurons. The activity of Na+/K+-ATPase and Ca2+-ATPase reduced in the MCAO group (3.67±0.48 U/mg,1.28±0.26 U/mg, respectively), and were significantly higher in WEA group (7.56±0.85 U/mg, F=12.65,P=0.010; 3.59±0.22 U/mg, F=8.32, P=0.041, respectively). The MCAO group showed significantly elevated P53 and HSP70 mRNA expressions compared to the sham group (P<0.01, P<0.05). P53 mRNA expressions in Agrimony extracts treated groups were significantly lower than that of the MCAO group (all P<0.01), with the WEA group showing the greatest difference from MCAO group. The HSP70 mRNA level of the treated groups were not significantly different from that of the MCAO group.Conclusions Treatment using water extracts of agrimony can promote the best functional and metabolic recovery for rat model of cerebral ischemia-reperfusion injury, which maybe relate with the upregulation of energy metabolism in nerve cells after MCAO. | Huiyuan Zhu Yulong Bie Jiang Wang Jing Gao Bingyue Yang Haitong Wan | 2017 | Chinese Medical Sciences Journal2017，32，4： | 3 |
| 15 | Wenyang Huazhuo Fang exerts transient receptor potential cation channel subfamily C member-dependent nephroprotection in a rat model of doxorubicin-induced nephropathy显示文摘OBJECTIVE:To determine the effect of Wenyang Huazhuo Fang(WHF),a Traditional Chinese Medicine decoction,on renal function in a rat model of doxorubicin-induced nephropathy,and to elucidate the underlying mechanism.METHODS:Sprague-Dawley rats were randomly divided into six groups:control,doxorubicin-nephropathy,and prednisone-treated(6.45 mg·kg^-1·d^-1)doxorubicin nephropathy groups,as well as high-(7.26 g·kg^-1·d^-1),medium-(2.42 g·kg-1·d-),and low-dose(0.81 g·kg^-1·d^-1)WHF-treated doxorubicin-nephropathy groups.The nephropathy rat model was established by two tail vein injections of doxorubicin,followed by prednisone or WHF treatment for 8 weeks.Body weights were monitored and urinary protein was measured every 2 weeks.After the end of the treatment period,the rats were euthanized.Serum biochemical indicators were determined and renal morphological alterations were assessed using histological staining.The expression of transient receptor potential cation channel subfamily C member 6(TRPC6),stromal interaction molecule 1(STIM1),and calcium release-activated calcium channel protein 1(Orai1)was detected using western blotting,and their mRNA levels were examined using quantitative real-time reverse transcription-polymerase chain reaction.RESULTS:WHF treatment was found to significantly ameliorate weight loss,proteinuria,hypoalbuminemia,and dyslipidemia in doxorubicin-nephropathy rats.The protein and mRNA levels of TRPC6,STIM1,and Orai1 were partially,but significantly suppressed by prednisone or WHF treatment.CONCLUSION:Treatment with WHF significantly ameliorates renal injury in a rat model of doxorubicin-induced nephropathy,which could be at least partially related to repression of the TRPC6 pathway. | An Peng Dong Sheng Li Xingyao Cai Zimo Ye Bingyu Zhang Aijun Shi Xingmin Wu Xili | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 3 |
| 16 | Quantifying the effects of stripe rust disease on wheat canopy spectrum based on eliminating non-physiological stresses显示文摘The wheat canopy reflectance spectrum is affected by many internal and external factors such as diseases and growth stage. Separating the effects of disease stress on the crop from the observed mixed signals is crucial for increasing the precision of remote sensing monitoring of wheat stripe rust. The canopy spectrum of winter wheat infected by stripe rust was processed with the difference-in-differences(DID) algorithm used in econometrics. The monitoring accuracies of wheat stripe rust before and after processing with the DID algorithm were compared in the presence of various external factors, disease severity, and several simulated satellite sensors. The correlation between the normalized difference vegetation index processed by the DID algorithm(NDVI-DID) and the disease severity level(SL) increased in comparison with the NDVI before processing. The increase in precision in the natural disease area in the field in the presence of large differences in growth stage, growth, planting, and management of the crop was greater than that in the controlled experiment. For low disease levels(SL < 20%), the R2 of the regression of NDVI-DIDon SL was 38.8% higher than that of the NDVI and the root mean square error(RMSE) was reduced by 11.1%. The increase in precision was greater than that for the severe level(SL > 40%).According to the measured hyperspectral data, the spectral reflectance of three satellite sensor levels was simulated. The wide-band NDVI was calculated. Compared with the wide-band NDVI and vegetation indexes(VI) before DID processing, there were increases in the correlation between SL and the various types of VIS-DID, as well as in the correlation between SL and NDVI-DID. It is feasible to apply the DID algorithm to multispectral satellite data and diverse types of VISfor monitoring wheat stripe rust. Our results improve the quantification of independent effects of stripe rust infection on canopy reflectance spectrum,increase the precision of remote sensing monitoring of wheat stripe rust, and provide a reference for remote sensing monitoring of other crop diseases. | Xia Jing Kaiqi Du Weina Duan Qin Zou Tingting Zhao Bingyu Li Qixing Ye Lieshen Yan | 2022 | The Crop Journal2022，10，5： | 3 |
| 17 | Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display library显示文摘AIM: To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naive scFv phage display library.METHODS: A large human naive scFv phage library was used to search for the specific targets by biopanning with the hepatocellular carcinoma cell line HepG2 for the positiveselecting and the normal liver cell line L02 for the counterselecting. After three rounds of biopanning, individual scFv phages binding selectively to HepG2 cells were picked out. PCR was carried out for identification of the clones containing scFv gene sequence. The specific scFv phages were selected by ELISA and flow cytometry. DNA sequences of positive clones were analyzed by using Applied Biosystem Automated DNA sequencers 3 730. The expression proteins of the specific scFv antibody fragments in E. coli HB2151were purified by the affinity chromatography and detected by SDS-PAGE, Western blot and ELISA. The biological effect of the soluble antibody fragments on the HepG2 cells was investigated by observing the cell proliferation.RESULTS: Two different positive clones were obtained and the functional variable sequences were identified.Their DNA sequences of the scFv antibody fragments were submitted to GenBank (accession nos: AY686498 and AY686499). The soluble scFv antibody fragments were successfully expressed in E. coli HB2151. The relative molecular mass of the expression products was about 36 ku,according to its predicted Mr value. The two soluble scFv antibody fragments also had specific binding activity and obvious growth inhibition properties to HepG2 cells.CONCLUSION: The phage library biopanning permits identification of specific antibody fragments for hepatocellular carcinoma and affords experiment evidence for its immunotherapy study. | BingYu MingNi Wen-HanLi PingLei WeiXing Dai-WenXiao YuHuang Zhen-JieTang Hui-FenZhu Guan-XinShen | 2005 | World Journal of Gastroenterology2005，11，26： | 2 |
| 18 | AN EXPERT SYSTEM FOR COMPUTER AIDED ALLOY STEEL DESIGN—ASPRES显示文摘An alloy steel pattern recognition expert system,ASPRES,has been established for thepurpose of computer aided optimal design of alloy steel in compositions and process pa-rameters,Pattern recognition techniques are used to abstract inner relationship betweenmechanical properties and process variables.The ASPRES uses 2-dimensional graph asvisual knowledge to represent domain expertise of specific object.Forward and back-ward chaining can be utilized by researcher in predicting sample performances or givinghelpful suggestions about the chemical compositions and process parameters accordingto desired properties. | CHEN Yu CHEN Nianyi Shanghai institute of Metallurgy,Academia Sinica,Shanghai,China PENG Ping KONG Bingyu Iron and Steel Research Institute,Shanghai Baoshan Iron and Steel Complex,Shanghai,China professor,Shanghai Institute of Metallurgy,Academia Sinica,Shanghai 200050,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，8： | 2 |
| 19 | Characterization of the immune defense related tissues,cells,and genes in amphioxus显示文摘Amphioxus is an important animal model for phylogenetic analysis,including comparative immunology.Exploring the immune system in amphioxus contributes to our understanding of the origin and evolution of the vertebrate immune system.We investigated the amphioxus immune system using ultrastructural examination and in situ hybridization.The expression patterns of TLR1(toll-like receptor 1),C1Q(complement component 1,q subcomponent),ECSIT(evolutionarily conserved signaling intermediate in Toll pathways),SoxC,DDAHa(Dimethylarginine dimethylaminohydrolase a),and NOS(nitric oxide synthase) show that these genes play key roles in amphioxus immunity.Our results suggest that the epidermis and alimentary canal epithelium may play important roles in immune defense,while macrophages located in the coelom and so-called lymph spaces may also be crucial immune cells. | LIN YuShuang CHEN DongYan ZHANG Wei CAI ZhaoPing CHEN ZhongKe ZHANG Ning MAO BingYu ZHANG HongWei | 2011 | Science China(Life Sciences)2011，54，11： | 2 |
| 20 | OTUD5 promotes innate antiviral and antitumor immunity through deubiquitinating and stabilizing STING显示文摘Stimulator of interferon genes(STING)is an adaptor protein that is critical for effective innate antiviral and antitumor immunity.The activity of STING is heavily regulated by protein ubiquitination,which is fine-tuned by both E3 ubiquitin ligases and deubiquitinases.Here,we report that the deubiquitinase OTUD5 interacts with STING,cleaves its K48-linked polyubiquitin chains,and promotes its stability.Consistently,knockout of OTUD5 resulted in faster turnover of STING and subsequently impaired type I IFN signaling following cytosolic DNA stimulation.More importantly,Lyz2-Cre Otud5^(fl/Y) mice and CD11-Cre Otud5^(fl/Y) mice showed more susceptibility to herpes simplex virus type 1(HSV-1)infection and faster development of melanomas than their corresponding control littermates,indicating that OTUD5 is indispensable for STING-mediated antiviral and antitumor immunity.Our data suggest that OTUD5 is a novel checkpoint in the cGAS-STING cytosolic DNA sensing pathway. | Yunyun Guo Fei Jiang Lingli Kong Haifeng Wu Honghai Zhang Xiaorong Chen Jian Zhao Baoshan Cai Yanqi Li Chunhong Ma Fan Yi Lei Zhang Bingyu Liu Yi Zheng Lingqiang Zhang Chengjiang Gao | 2021 | Cellular & Molecular Immunology2021，18，8： | 2 |
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