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| 1 | 3D Chromatin Architecture of Large Plant Genomes Determined by Local A/B Compartments显示文摘染色体的空间组织在基因表示的规定起一个重要作用。然而,核心动物染色体的结构的特征例如拓扑地联系的域(男孩) 和染色质环,不在极其紧缩的 Arabidopsis 染色体是突出的。在这研究,我们检验 ? 染色质建筑学,以及他们的 DNA methylation, histone 修正,可存取的染色质,和基因表示玉米,有染色体的西红柿,蜀黍,狐尾稷,和米饭缩放从 0.4 ? 到 2.4 Gb。我们发现这些植物染色体能被划分成象哺乳动物一样 A/B 分隔空间。在更高的分辨率,这些植物的染色体能进一步被划分到反映他们的 euchromatin, heterochromatin,和 polycomb 地位的本地 A/B 分隔空间。在所有这些植物的染色质被组织成没越过种类被保存的领域。他们显示出类似到果蝇分隔空间领域,并且被聚类进活跃, polycomb,压抑、中间的类型基于他们的 transcriptional 活动和 epigenetic 签名,与有本地 A/B 分隔空间连接的领域边阶重叠。在大玉米和西红柿染色体,我们观察了广泛的染色质环。不同于在 TAD 边阶被充实的哺乳动物的染色质环,然而种染色质环经常在压抑的域外面在基因岛之间被形成 ? 并且仔细与活跃分隔空间被联系。我们的学习显示植物有复杂、唯一的 3D 染色质体系结构,它要求 ? 阐明的进一步的学习他们的生物功能。 | Pengfei Dong Xiaoyu Tu Po-Yu Chu Peitao Lu Ning Zhu Donald Grierson Baijuan Du Pinghua Li Silin Zhong | 2017 | Molecular Plant2017，10，12： | 12 |
| 2 | On the modification of hypereutectic Al-Si alloys using rare earth Er显示文摘Hypereutectic Al-17.5Si (wt pct) and Al-25Si (wt pct) alloys with various content of rare earth Er were prepared by conventional casting technique. The effect of Er on the microstructure and properties of the hypereutectic Al-Si alloys was investigated using optical microscopy, scanning electron microscopy as well as friction and wear tests. It was found that optimal amount of Er exists in modifying the hypereutectic Al-Si alloys. An appropriate addition of Er improved the anti-wear properties, and reduced the friction coefficient of the hypereutectic Al-Si alloys studied. The primary silicon crystals in the hypereutectic Al-Si alloys were refined with the suitable addition of Er element. The modification mechanism was also discussed in this paper. | Pengfei XING Bo GAO Yanxin ZHUANG Kaihua LIU Ganfeng TU | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，5： | 7 |
| 3 | Application of ~1H NMR-based metabolomics for discrimination of different parts and development of a new processing workflow for Cistanche deserticola显示文摘Cistanche deserticola(CD) is one of the two authoritative source plants of Cistanches Herba, a well-known medicinal plant. Herein,~1H NMR spectroscopy was employed to characterize the chemical profile and to distinguish the different parts, as well as to propose a new processing workflow for CD.Signal assignment was achieved by multiple one and two dimensional NMR spectroscopic techniques in combination with available databases and authentic compounds. The upper parts of the plant were distinguished from the lower parts by combining ~1H NMR spectroscopic dataset with multivariate statistical analysis. A new processing method that hyphenated steaming with freeze-drying, was demonstrated to be superior to either steaming coupled with oven-drying or direct freeze-drying via holistic ~1H NMR-based metabolomic characterization. Phenylethanoid glycosides, mainly echinacoside and acteoside, were screened out and confirmed as the chemical markers responsible for exhibiting the superiority of the new processing workflow, whereas serial primary metabolites, especially carbohydrates and tricarboxylic acid cycle metabolites, were found as the primary molecules governing the discrimination between the upper and lower parts of the plant. Collectively,~1H NMR spectroscopy was demonstrated as a versatile analytical tool to characterize the chemical profile and to guide the indepth exploitation of CD by providing comprehensive qualitative and quantitative information. | Pingping Zou Yuelin Song Wei Lei Jun Li Pengfei Tu Yong Jiang | 2017 | Acta Pharmaceutica Sinica B2017，7，6： | 7 |
| 4 | Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography–nuclear magnetic resonance spectroscopy–tandem mass spectrometry显示文摘Herein, a strategy is proposed for the simultaneous determination of primary coumarins in Peucedani Radix(Chinese name: Qianhu). The methodology consists of three consecutive steps: 1) Semi-preparative LC in combination with a home-made automated fraction collection module to fragment the universal metabolome standard into ten fractions(Frs. I–X); 2) LC–accurate MS/MS and quantitative1 H NMR spectroscopy conducted in parallel to acquire the qualitative and quantitative data of each fraction; 3) Robust identification and quantification of components by use of LC coupled to multiple reaction monitoring. In this final step, the most significant fractions(Frs. III–X) were pooled to serve as the pseudo-mixed standard solution. Meticulous online parameter optimization was performed to obtain the optimal parameters, including ion transitions and collision energies. Concerns were particularly paid onto pursuing the parameters being capable of monitoring regiospecific isomers, notably praeruptorin E vs. 3′-isovaleryl-4′-angeloylkhellactone. The quantitative performance of the method was validated according to diverse assays. Eleven primary coumarins(1–11) were unambiguously identified and absolutely quantified, even though no external reference compound was used. Above all, the integrated strategy not only provides a feasible pipeline for the quality assessment of Peucedani Radix, but more importantly, shows the potential for authentic compound-free quantitative evaluation of traditional Chinese medicines. | Yao Liu Qingqing Song Wenjing Liu Peng Li Jun Li Yunfang Zhao Liang Zhang Pengfei Tu Yitao Wang Yuelin Song | 2018 | Acta Pharmaceutica Sinica B2018，8，4： | 6 |
| 5 | Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase显示文摘Diabetes have been shown to cause progressive neuronal injury with pain and numbness via advanced glycation end-products(AGEs)-induced neuronal cell apoptosis;however, the valuable drug targets for diabetic neuropathy have been poorly reported so far. In this study, we discovered a natural small-molecule schisandrol A(SolA) with significant protective effect against AGEs-induced neuronal cell apoptosis. ATP6V0D1, a major subunit of vacuolar-type ATPase(V-ATPase) in lysosome was identified as a crucial cellular target of SolA. Moreover, SolA allosterically mediated ATP6V0D1 conformation via targeting a unique cysteine 335 residue to activate V-ATPase-dependent lysosomal acidification.Interestingly, SolA-induced lysosome pH downregulation resulted in a mitochondrial-lysosomal crosstalk by selectively promoting mitochondrial BH3-only protein BIM degradation, thereby preserving mitochondrial homeostasis and neuronal cells survival. Collectively, our findings reveal ATP6V0D1 is a valuable pharmacological target for diabetes-associated neuronal injury via controlling lysosomal acidification, and also provide the first small-molecule template allosterically activating V-ATPase for preventing diabetic neuropathy. | Xiaoqing Zhou Shaoyang Zhao Tingting Liu Lu Yao Meimei Zhao Xiaoming Ye Xiaowen Zhang Qiang Guo Pengfei Tu Kewu Zeng | 2022 | Acta Pharmaceutica Sinica B2022，12，10： | 3 |
| 6 | Small molecule induces mitochondrial fusion for neuroprotection via targeting CK2 without affecting its conventional kinase activity显示文摘Mitochondrial fusion/hission dynamics plays a fundamental role in neuroprotection;however,there is till a severe lack oftherapeutic targets for this biological process.Here,we found that the naturally derived small molecule echinacoside(ECH)signifhcantly promotes mitochondrial fusion progression.ECH selectively binds to the previously uncharacterized casein kinase 2(CK2)α'subunit(CK2a)as a direct cellular target,and genetic knockdown of CK2α'abolishes ECH-mediated mitochondrial fusion.Mechanistically,ECH allosterically regulates CK2α'conformation to recruit basic transcription factor 3(BTF 3)to form a binary proteincomplex.Then,the CK2α'/BTF3 complex facilitatesβ-catenin nuclear translocation to activate TCF/AEF transcription factors andstimulate transcription of the mitochondrial fusion gene Mfn2.trikingly,in a mouse middle cerebral artery occlusion(MCAO)model,ECH administration was found to significantly improve cerebral injuries and behavioral deficits by enhancing Mfn2expression in wild-type but not CK2α'^(+/-)mice.Taken together,our findings reveal,for the first time,that CK2 is essential forpromoting mitochondrial fusion in a Wnt/B-catenin-dependent manner and suggest that pharmacologically targeting CK2 is apromising therapeutic strategy for ischemic stroke. | Ke-Wu Zeng Jing-Kang Wang Li-Chao Wang Qiang Guo Ting-Ting Liu Fu-Jiang Wang Na Feng Xiao-Wen Zhang Li-Xi Liao Mei-Mei Zhao Dan Liu Yong Jiang Pengfei Tu | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 3 |
| 7 | Recover the abnormal positioning,velocity and timing services caused by BDS satellite orbital maneuvers显示文摘The BeiDou Navigation Satellite System(BDS)provides global Positioning,Velocity,And Timing(PVT)services that are widely used in various areas.The BDS satellites frequently need the orbit maneuvers due to various perturbations to keep satellites in their designed positions.During these maneuvers,PVT services may be abnormal if the data from a maneuvering satellite is used.In this paper we developed an approach to recover the abnormal PVT services.By using BDS observations from multiple tracking stations,the orbital errors of a maneuvering satellite can be in real time obtained and corrected,thereby avoiding any influence on the performance of PVT services.The tests show that the average precision of position,velocity and timing services are improved by 0.8 m,0.1 mm/s and 0.16 ns,respectively,using the developed orbital maneuver recovery approach.In addition,the approach can also be used for the orbital maneuver detection and monitoring. | Rui Tu Rui Zhang Pengfei Zhang Junqiang Han Lihong Fan Xiaochun Lu | 2021 | Satellite Navigation2021，2，1： | 3 |
| 8 | Four new phenolic glycosides from Baoyuan decoction显示文摘Four new phenolic glycosides,including two flavonoid glycosides(1 and 2) and two lignan glycosides(3 and 4),were isolated from the traditional Chinese medicine formula,Baoyuan decoction.Their structures were established by detailed analysis of the NMR and HR-ESI-MS spectroscopic data and their absolute configurations were determined by the experimental electronic circular dichroism data as well as chemical methods.Furthermore,the sources of the four new compounds were determined by the UPLC-Qtrap-MS method,which proved that 1 and 2 are originated from Glycyrrhiza uralensis,and 3 and4 are from Cinnamomum cassia. | Xiaoli Ma Xiaoyu Guo Mingbo Zhao Pengfei Tu Yong Jiang | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 2 |
| 9 | Neutralization of SARS-CoV-2 pseudovirus using ACE2-engineered extracellular vesicles显示文摘The spread of coronavirus disease 2019(COVID-19)throughout the world has resulted in stressful healthcare burdens and global health crises.Developing an effective measure to protect people from infection is an urgent need.The blockage of interaction between angiotensin-converting enzyme 2(ACE2)and S protein is considered an essential target for anti-severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)drugs.A full-length ACE2 protein could be a potential drug to block early entry of SARS-Co V-2 into host cells.In this study,a therapeutic strategy was developed by using extracellular vesicles(EVs)with decoy receptor ACE2 for neutralization of SARS-Co V-2.The EVs embedded with engineered ACE2(EVs-ACE2)were prepared;the EVs-ACE2 were derived from an engineered cell line with stable ACE2 expression.The potential effect of the EVs-ACE2 on anti-SARS-Co V-2 was demonstrated by both in vitro and in vivo neutralization experiments using the pseudovirus with the S protein(S-pseudovirus).EVs-ACE2 can inhibit the infection of S-pseudovirus in various cells,and importantly,the mice treated with intranasal administration of EVs-ACE2 can suppress the entry of S-pseudovirus into the mucosal epithelium.Therefore,the intranasal EVs-ACE2 could be a preventive medicine to protect from SARS-Co V-2 infection.This EVs-based strategy offers a potential route to COVID-19 drug development. | Canhao Wu Qin Xu Huiyuan Wang Bin Tu Jiaxin Zeng Pengfei Zhao Mingjie Shi Hong Qiu Yongzhuo Huang | 2022 | Acta Pharmaceutica Sinica B2022，12，3： | 2 |
| 10 | Uncertainties in stormwater runoff data collection from a small urban catchment, Southeast China显示文摘Monitoring data are often used to identify stormwater runoff characteristics and in stormwater runoff modelling without consideration of their inherent uncertainties. Integrated with discrete sample analysis and error propagation analysis, this study attempted to quantify the uncertainties of discrete chemical oxygen demand (COD), total suspended solids (TSS) concentration, stormwater flowrate, stormwater event volumes, COD event mean concentration (EMC), and COD event loads in terms of flow measurement, sample collection, storage and laboratory analysis. The results showed that the uncertainties due to sample collection, storage and laboratory analysis of COD from stormwater runoff are 13.99%, 19.48% and 12.28%. Meanwhile, flow measurement uncertainty was 12.82%, and the sample collection uncertainty of TSS from stormwater runoff was 31.63%. Based on the law of propagation of uncertainties, the uncertainties regarding event flow volume, COD EMC and COD event loads were quantified as 7.03%, 10.26% and 18.47%. | Jinliang Huan Zhenshun Tu Pengfei Du Jie Lin Qingsheng Li | 2010 | Journal of Environmental Sciences2010，22，11： | 2 |
| 11 | Pomegranate-type Si/C anode with SiC taped,well-dispersed tiny Si particles for lithium-ion batteries显示文摘Severe volume expansion and inherently poor lithium ion transmission are two major problems of silicon anodes.To address these issues,we proposed a pomegranate-type Si/C composite anode with highly dispersed tiny silicon particles as the core assisted by small amount of SiC.Skillfully exploiting the high heat from magnesiothermic reduction,SiC can assist the good dispersion of silicon and provide good interface compatibility and chemical stability.The silicon anchored to the carbon shell provides multipoint contact mode,that together with the carbon shell frame,significantly promoting the transfer of dual charge.Besides,the pomegranate-type microcluster structure also improves the tap density of the electrode,reduces the direct contact area between active material and electrolyte,and enhances the electrochemical performance. | Pengfei WU Benyang SHI Huibin TU Changqing GUO Anhua LIU Guan YAN Zhaoju YU | 2021 | Journal of Advanced Ceramics2021，10，5： | 2 |
| 12 | Analysis of chemical constituents in Cistanche species显示文摘 | JIANG Yong TU Pengfei | 2009 | Journal of Chromatography A2009，1216，11： | 1 |
| 13 | Effects of parasitic plant Cistanche deserticola on chlorophyll a fluorescence and nutrient accumulation of host plant Haloxylon ammodendron in the Taklimakan Desert显示文摘The parasitic plant Cistanche deserticola attaches to Haloxylon ammodendron, a perennial shrub with high tolerance to salinity and drought. However, little was known about the parasite-host relation between the two species. Effects of the parasite on chlorophyll a fluorescence and nutrient accumulation in the host plant (H. ammodendron) were investigated in the Taklimakan Desert. Some photosynthetic parameters of both host and non-host H. ammodendron plants were measured by in vivo chlorophyll a fluorescence technology in the field. The assimilating branches of host and non-host plants were collected and nutrient and inorganic ion contents were analyzed. The results from field experiments showed that the infection of C. deserticola reduced the non-photochemical quenching of the variable chlorophyll fluorescence (NPQ) and the potential maximum quantum yield for primary photochemistry (F v /F m ) of the host. Compared with non-host plants, the host H. ammodendron had low nutrient, low inorganic ion contents (Na + and K + ) and low K + /Na + ratios in the assimilating branches. It suggested that C. deserticola infection reduced the nutrient acquisition and caused damage to the photoprotection through thermal dissipation of the energy of the photosystem II in the host, resulting in a decrease in the tolerance to salinity and high radiation. It was concluded that the attachment of the parasite plant (C. deserticola) had negative effects on the growth of its host. | Li LI XinWen XU YongQiang SUN WeiHAN PengFei TU | 2012 | Journal of Arid Land2012，4，3： | 1 |
| 14 | Probing the active sites of 2D nanosheets with Fe-N-C carbon shell encapsulated Fe_(x)C/Fe species for boosting sodium-ion storage performances显示文摘Developing stable but high active metal-nitrogen-carbon(M-N-C)-based hard carbon anode is a promising way to be the alternatives to graphene and blank hard carbon for sodium-ion batteries(SIBs),requiring the precise tailoring of the electronic structure for optimizing the Na+intercalation behavior,yet is greatly challenging.Herein,Fe-N-C graphitic layer-encapsulating Fe3C species within hard carbon nanosheets(Fe-N-C/Fe3C@HCNs)are rationally engineered by pyrolysis of self-assembled polymer.Impressively,the Fe-N-C/Fe3C@HCNs exhibit outstanding rate capacity(242 mAh·g^(−1)at 2,000 mA·g^(−1)),which is 2.1 and 4.2 times higher than that of Fe-N-C and N-doped carbon(N-C),respectively,and prolonged cycling stability(176 mAh·g^(−1)at 2,000 mA·g^(−1)after 2,000 cycles).Theoretical calculations unveil that the Fe3C species enhance the electronic transfer from Na to Fe-N-C,resulting in the charge redistribution between the interfaces of Fe3C and Fe-N-C.Thus,the optimized adsorption behavior towards Na+reduces the thermodynamic energy barriers.The synergistic effect of Fe3C and Fe-N-C species maintains the structural integrity of electrode materials during the sodiation/desodiation process.The in-depth insight into the advanced Na+storage mechanisms of Fe3C@Fe-N-C offers precise guidance for the rational establishment of confinement heterostructures in SIBs. | Huicong Xia Pengfei Yuan Lingxing Zan Gan Qu Yunchuan Tu Kaixin Zhu Yifan Wei Zeyu WeiFangying Zheng Mo Zhang Yongfeng Hu Dehui Deng Jianan Zhang | 2022 | Nano Research2022，15，8： | 1 |
| 15 | Characterization of water soluble polysaccharides from organs of Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao)显示文摘 | Zhihui Zhao Mengjun Liu Pengfei Tu | 2008 | European Food Research and Technology2008，，5： | 1 |
| 16 | Xylocoside G Reduces Amyloid-β Induced Neurotoxicity by Inhibiting NF-κB Signaling Pathway in Neuronal Cells显示文摘 | Yan Yu Liang Zhou Miao Sun Ting Zhou Kaiyin Zhong Hecheng Wang Yi Liu Xinying Liu RuiZhong Xiao Jia Ge PengFei Tu Dong Sheng Fan Yuan Lan Chen Hui Dehua Chui | 2012 | Journal of Alzheimer’s Disease2012，，2： | 1 |
| 17 | Analysis of chemical constituents in Cistanche species显示文摘 | JIANG Yong TU Pengfei | 2009 | Journal of Chromatography A2009，1216，11： | 1 |
| 18 | A SERIES OF STRATEGIES FOR SOLVING THE SHORTAGE OF REFERENCE STANDARDS FOR MULTI-COMPONENTS DETERMINATION OF TRADITIONAL CHINESE MEDICINE显示文摘The shortage of reference standards has become the bottleneck of quality control of TCMs.In this study,a series of strategies,including one single reference standard to determine multi-compounds(SSDMC),quantitative analysis by standardized reference extract(QASRE),and quantitative nuclear magnetic resonance | Wenguang Wang Xiaoli Ma Pengfei Tu Yong Jiang | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 1 |
| 19 | A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus , a Chinese medicinal herb显示文摘 | Bao-Mei Shao Wen Xu Hui Dai Pengfei Tu Zhongjun Li Xiao-Ming Gao | 2004 | Biochemical and Biophysical Research Communications2004，，4： | 1 |
| 20 | Characterization of water soluble polysaccharides from organs of Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao)显示文摘 | Zhihui Zhao Mengjun Liu Pengfei Tu | 2008 | European Food Research and Technology2008，，5： | 1 |
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