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| 1 | Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization显示文摘AIM: To determine the role of interleukin (IL)-17 in gastric ulcerogenesis. METHODS: Thirty-six gastric ulcer (GU) patients and 29 non-ulcer (NU) patients were enrolled in this study. Mucosal biopsy samples were obtained from the gastric antrum and GU site during endoscopy. Samples were used in in situ stimulation for 48 h in the presence of 10 μg/mL phytohemagglutinin-P (PHA), histological examination, and Helicobacter pylori(H pylori) culture. IL-17 and IL-8 protein levels in culture supernatants were assayed by ELISA. IL-17 mRNA expression was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). H pylori cagA and vacA status was assessed by reverse hybridization using a line probe assay (LiPA). IL-8 levels in culture supernatants were assayed after AGS cells were co-cultured with H pylori strain 26 695 or recombinant human (rh) IL-17. RESULTS: All 36 GU patients and 15 of 29 NU patients were found to be H pylori-positive, while 14 NU patients were H pylori-negative. All 51 H pylori strains from both GU and NU patients were cagA- and vacAs1/m1-positive. Antral mucosal tissues from H pylori-positive patients contained significantly (H pylori-positive NU patients: median 467 pg/mg/protein, range 53-2 499; H pylori-negative NU patients: median 104 pg/mg/protein, range 16-312, P<0.0005) higher levels of IL-17 than those from uninfected patients. IL-17 levels at the ulcer site were significantly (ulcer site: median 1356 pg/mg/protein, range 121-1 3730; antrum: median 761 pg/mg/protein, range 24-7 620, P<0.005) higher than those at distant sites in the antrum. Biopsies from H pylori-positive GU and NU patients showed IL-17 mRNA expression in all samples whereas those from the antrum of the H pylori-negative controls showed no detectable expression. A significant correlation was seen between IL-17 and IL-8 levels at each biopsy site (ulcer: r= 0.62, P<0.0001; antrum: r = 0.61, P<0.0001) in GU patients. RhIL-17 and H pylori strain 26 695 each stimulated IL-8 production from AGS cells. CONCLUSION: IL-17 may play an important role in the inflammatory response to H pylori colonization, and may ultimately influence the outcome of H pylori-associated diseases that arise within the context of gastritis. | Tomokazu Mizuno Takafumi Ando Kazuo Nobata Tomoyuki Tsuzuki Osamu Maeda Osamu Watanabe Masaaki Minami Kenji Ina Kazuo Kusugami Richard M. Peek Hidemi Goto | 2005 | World Journal of Gastroenterology2005，11，40： | 18 |
| 2 | 卒中相关性肺炎的诊断卒中肺炎共识小组的推荐意见显示文摘背景和目的下呼吸道感染常并发于卒中并对转归造成不利影响。对于并发于卒中的下呼吸道感染性疾病,目前尚无公认的专业术语或诊断金标准,这导致临床诊疗和研究受到影响。本共识旨在提出急性卒中并发下呼吸道感染的标准化术语和可操作性诊断标准。方法对多个文献数据库进行系统检索,经过证据回顾和2轮讨论,最后于2014年9月在英国曼彻斯特召开共识会议,经75%以上共识小组成员达成一致意见后形成共识。结果达成的共识如下:(1)推荐使用卒中相关性肺炎(stroke-associated pneumonia, SAP )作为卒中发病后7 d内发生的下呼吸道感染性疾病的专业术语。(2)提议对 SAP 使用经过改良的美国疾病控制预防中心(Centers for Disease Control and Prevention, CDC)诊断标准:①很可能的 SAP:符合 CDC 诊断标准,但复查或动态监测胸部 X 片仍缺乏典型改变;②确诊的 SAP:符合 CDC 诊断标准,且胸部 X 片有典型改变。(3)关于白细胞计数或 C反应蛋白在 SAP 诊断中的作用证据有限。(4)其他生物学标记物(如降钙素原)的证据不足。结论提议根据 CDC 诊断标准对 SAP 的术语和诊断制定可操作性的共识标准,需要在卒中患者中进行前瞻性研究来确定其信度、效度以及对医生行为(包括抗生素选择)和临床转归的影响。 | Craig J. Smith Amit K Kishore Andy Vail Angel Chamorro Javier Garau Stephen J. Hopkins Mario Di Napoli Lalit Kalra Peter Langhorne Joan Montaner Christine Roffe Anthony G. Rudd Pippa J. TyrreU Diederik van de Peek Mark Woodhead Andreas Meisel 范琳琳 宿英英 | 2016 | 国际脑血管病杂志2016，24，2： | 17 |
| 3 | Bacterial biota in reflux esophagitis and Barrett's esophagus显示文摘AIM: To identify the bacterial flora in conditions such as Barrett's esophagus and reflux esophagitis to determine if they are similar to normal esophageal flora.METHODS: Using broad-range 16S rDNA PCR,esophageal biopsies were examined from 24 patients [9with normal esophageal mucosa, 12 with gastroesophageal reflux disease (GERD), and 3 with Barrett's esophagus].Two separate broad-range PCR reactions were performed for each patient, and the resulting products were cloned.In one patient with Barrett's esophagus, g9 PCR clones were analyzed.RESULTS: Two separate clones were recovered from each patient (total = 48), representing 24 different species, with 14 species homologous to known bacteria,5 homologous to unidentified bacteria, and 5 were not homologous (<97% identity) to any known bacterial 16S rDNA sequences. Seventeen species were found in the reflux esophagitis patients, 5 in the Barrett's esophagus patients, and 10 in normal esophagus patients.Further analysis concentrating on a single biopsy from an individual with Barrett's esophagus revealed the presence of 21. distinct bacterial species. Members of four phyla were represented, including Bacteroidetes,Firmicutes, Proteobacteria, and Actinobacteria.Microscopic examination of each biopsy demonstrated bacteria in intimate association with the distal esophageal epithelium, suggesting that the presence of these bacteria is not transitory.CONCLUSION: These findings provide evidence for a complex, residential bacterial population in esophageal reflux-related disorders. While much of this biota is present in the normal esophagus, more detailed comparisons may help identify potential disease associations. | Zhiheng Pei Liying Yang Richard M Peek Jr Steven M Levine David T Pride Martin J Blaser | 2005 | World Journal of Gastroenterology2005，11，46： | 11 |
| 4 | The mating system of the true fruit fly Bactrocera tryoni and its sister species, Bactrocera neohumeralis显示文摘以果实为食的真水果苍蝇, Bactrocera tryoni (Queensland 水果苍蝇) ,被假定有基于 nonresourced 的列克交配系统。这大部分未经测试、矛盾数据存在建议 Bactrocera tryoni 可以把一个基于资源的交配系统集中于 fruiting 主机植物。我们测试了 Bactrocera tryoni,和它的靠近的兄弟 Bactrocera neohumeralis 的交配系统,在用实验室的大地笼子饲养的苍蝇。我们使用了允许我们决定是否的观察实验:交配对的(i) 被聚集或 nonaggregated;(ii ) 交配系统是基于的资源或 nonresource;(iii ) 苍蝇利用了可能的里程碑(高树在上短) 作为配偶约会地点;并且(iv ) 男性们从男性主导的列克打电话给女性。我们记录了越过所有实验交配对的将近 250 Bactrocera tryoni,揭示那:交配对的(i) 被聚集;(ii ) 交配几乎总是发生在高树上在上短;(iii ) 交配是基于的 nonresource;并且(iv ) 男性和女性和男性们先于女性的没有证据到达了配偶约会地点。Bactrocera neohumeralis 交媾是更很少发生的(仅仅总共的 30 交配对) ,要不是那些对,为高树和一个基于资源的交配系统的没有证据有类似的偏爱。交配行为的 Bactrocera tryoni 的一些方面与一个 lekking 系统的理论期望排列,但是其它不做。直到为不含糊的女性的证据,选择能被提供(是在真列克下面预言了) , Bactrocera tryoni 的交配系统最好被描述为基于的 nonresource,我们也为有 land-marking 可以被包含的证据的聚集系统。 | Wasala M. T. D. Ekanayake Mudalige S. H. Jayasundara Thelma Peek Anthony R. Clarke Mark K. Schutze | 2017 | Insect Science2017，24，3： | 3 |
| 5 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 6 | Diet, Helicobacter pylori Strain-Specific Infection, and Gastric Cancer Risk Among Chinese Men显示文摘 | Meira Epplein Wei Zheng Honglan Li Richard M. Peek Pelayo Correa Jing Gao Angelika Michel Michael Pawlita Qiuyin Cai Yong-Bing Xiang Xiao-Ou Shu | 2014 | Nutrition and Cancer2014，，： | 2 |
| 7 | Diet, microbial virulence, and Helicobacter pylori-induced gastric cancer显示文摘 | Timothy L Cover Richard M Peek Jr | 2013 | Gut Microbes2013，，： | 2 |
| 8 | Gastrointestinal Malignancy and the Microbiome显示文摘 | Maria T. Abreu Richard M. Peek | 2014 | Gastroenterology2014，，： | 2 |
| 9 | Vacuolating Cytotoxin and Variants in Atg16L1 That Disrupt Autophagy Promote Helicobacter pylori Infection in Humans显示文摘 | Deepa Raju Seamus Hussey Michelle Ang Mauricio R. Terebiznik Michal Sibony Esther Galindo–Mata Vijay Gupta Steven R. Blanke Alberto Delgado Judith Romero–Gallo Mahendra Singh Ramjeet Heidi Mascarenhas Richard M. Peek Pelayo Correa Cathy Streutker Georgina | 2012 | Gastroenterology2012，，5： | 2 |
| 10 | Efficacy and economic assessment of conventional ventilatory support versus extracorporeal membrane oxygenation for severe adult respiratory failure(CESAR):a multicentre randomised controlled trial显示文摘 | Peek GJ Mugford M Tiruvoipati R | 2009 | Lancet2009，374，9698： | 1 |
| 11 | Helicobacter pylori and Gastroesophageal Reflux Disease显示文摘 | Peek RM | 2004 | Curr Treat Options Gastroenterol2004，7，： | 1 |
| 12 | Clinical and pathological importance of heterogenecity in vacA,the vacuolating cytotoxin gene of Helicobacter pylori显示文摘 | Atherton JC Peek RM Jr Tham KT | 1997 | Gastroenterology1997，112，： | 1 |
| 13 | Dynamic loads in the interior of a layered stratum: an explicit solution显示文摘 | Kausel E Peek R | 1982 | Bulletin of the Seismological Society of America1982，72，5： | 1 |
| 14 | Helicobacter pylori and gastrointestinal adenocarcinomas显示文摘 | Peek R M Jr blaser M J | 2002 | Nat Res Cancer2002，2，1： | 1 |
| 15 | 26. Pain in Chronic Pancreatitis显示文摘 | Martine Puylaert Leonardo Kapural Jan Van Zundert Dirk Peek Arno Lataster Nagy Mekhail Maarten van Kleef Yolande C.A. Keulemans | 2011 | Pain Practice2011，，5： | 1 |
| 16 | Eco- nomics of Disaster Risk, Social Vulnerability, and Mental Health Resilience显示文摘 | Zahran S Peek L Snodgrass J G Weiler S Hempel L | 2011 | Risk Analysis2011，31，7： | 1 |
| 17 | FDA approves ues of bacteriophages to be added to meat and poultry products显示文摘 | Peek R Rajender K R | 2006 | Gastroenterology2006，131，5： | 1 |
| 18 | Interleukin-lO-Secreting “regulatory” T Cells Induced by glucocorticoids and beta2-agonists 显示文摘 | Peek EJ Richards DF Faith A | 2005 | Am J Respir Cell Mol Biol2005，33，1： | 1 |
| 19 | Regulation of the human catalytic subunit of telomerase (hTERT) 显示文摘 | Daniel M Peek G W Tollefsbol T O | 2012 | Gene2012，498，2： | 1 |
| 20 | Dynamic loads in the interior of a layered stratum:an explicit solution显示文摘 | KAUSEL E PEEK R | 1982 | Bull Seismol Soc Am1982，72，5： | 1 |
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