|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Optimized Nonbismuth Quadruple Therapies Cure Most Patients With Helicobacter pylori Infection in Populations With High Rates of Antibiotic Resistance显示文摘 | Javier Molina–Infante Marco Romano Miguel Fernandez–Bermejo Alessandro Federico Antonietta G. Gravina Liliana Pozzati Elena Garcia–Abadia Gema Vinagre–Rodriguez Carmen Martinez–Alcala Moises Hernandez–Alonso Agnese Miranda Maria Rosaria Iovene Carmen Pazo | 2013 | Gastroenterology2013，，1： | 8 |
| 2 | In vitro effect of amoxicillin and clarithromycin on the 3' region of cagA gene in Helicobacter pylori isolates显示文摘AIM:To evaluate the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island(cag PAI).METHODS:One hundred and forty-nine clinical isolates of Helicobacter pylori(H.pylori)cultured from gastric biopsies from 206 Colombian patients with dyspeptic symptoms from a high-risk area for gastric cancer were included as study material.Antimicrobial susceptibility was determined by the agar dilution method.Resistant isolates at baseline and in amoxicillin and clarithromycin serial dilutions were subjected to genotyping(cagA,vacA alleles s and m),Glu-Pro-Ile-Tyr-Ala(EPIYA)polymerase chain reaction and random amplified polymorphic DNA(RAPD).Images of the RAPD amplicons were analyzed by Gel-Pro Analyzer 4.5program.Cluster analyses was done using SPSS 15.0statistical package,where each of the fingerprint bands were denoted as variables.Dendrograms were designed by following Ward’s clustering method and the estimation of distances between each pair of H.pylori isolates was calculated with the squared Euclidean distance.RESULTS:Resistance rates were 4%for amoxicillin and 2.7%for clarithromycin with 2%double resistances.Genotyping evidenced a high prevalence of the genotype cagA-positive/vacA s1m1.The 3’region of cagA gene was successfully amplified in 92.3%(12/13)of the baseline resistant isolates and in 60%(36/60)of the resistant isolates growing in antibiotic dilutions.Upon observing the distribution of the number of EPIYA repetitions in each dilution with respect to baseline isolates,it was found that in 61.5%(8/13)of the baseline isolates,a change in the number of EPIYA repetitions lowered antibiotic pressure.The gain and loss of EPIYA motifs resulted in a diversity of H.pylori subclones after bacterial adjustment to changing conditions product of antibiotic pressure.RAPD PCR evidenced the close clonal relationship between baseline isolates and isolates growing in antibiotic dilutions.CONCLUSION:Antibiotic pressure does not induce loss of the cag pathogenicity island,but it can leadin most cases-to genetic rearrangements within the3’region cagA of the founding bacteria that can affect the level of tyrosine phosphorylation impacting on its cellular effects and lead to divergence of cagA-positive subclones. | Javier Andrés Bustamante-Rengifo Andrés Januer Matta Alvaro Pazos Luis Eduardo Bravo | 2013 | World Journal of Gastroenterology2013，19，36： | 5 |
| 3 | Punctual mutations in 23S rRNA gene of clarithromycinresistant Helicobacter pylori in Colombian populations显示文摘AIM To characterize punctual mutations in 23S rRNA gene of clarithromycin-resistant Helicobacter pylori(H. pylori) and determine their association with therapeutic failure.METHODS PCR products of 23S rRNA gene V domain of 74 H. pylori isolates; 34 resistant to clarithromycin(29 from a low-risk gastric cancer(GC) population: TumacoColombia, and 5 from a high-risk population: TuquerresColombia) and 40 from a susceptible population(28 from Tumaco and 12 from Túquerres) were sequenced using capillary electrophoresis. The concordance between mutations of V domain 23S rRNA gene of H. pylori and therapeutic failure was determined using the Kappa coefficient and Mc Nemar's test was performed to determine the relationship between H. pylori mutationsand clarithromycin resistance.RESULTS23S rRNA gene from H. pylori was amplified in 56/74 isolates, of which 25 were resistant to clarithromycin(20 from Tumaco and 5 from Túquerres, respectively). In 17 resistant isolates(13 from Tumaco and 4 from Túquerres) the following mutations were found: A1593 T1, A1653 G2, C1770 T, C1954 T1, and G1827 C in isolates from Tumaco, and A2144 G from Túquerres. The mutations T2183 C, A2144 G and C2196 T in H. pylori isolates resistant to clarithromycin from Colombia are reported for the first time. No association between the H. pylori mutations and in vitro clarithromycin resistance was found. However, therapeutic failure of eradication treatment was associated with mutations of 23S rRNA gene in clarithromycin-resistant H. pylori(κ = 0.71).CONCLUSION The therapeutic failure of eradication treatment in the two populations from Colombia was associated with mutations of the 23S rRNA gene in clarithromycinresistant H. pylori. | Andrés Jenuer Matta Diana Carolina Zambrano Alvaro Jairo Pazos | 2018 | World Journal of Gastroenterology2018，24，14： | 5 |
| 4 | Nonbismuth Quadruple (Concomitant) Therapy: Empirical and Tailored Efficacy versus Standard Triple Therapy for Clarithromycin‐Susceptible Helicobacter pylori and versus Sequential Therapy for Clarithromycin‐Resistant Strains显示文摘 | Javier Molina‐Infante Carmen Pazos‐Pacheco Gema Vinagre‐Rodriguez Belen Perez‐Gallardo Carmen Due?as‐Sadornil Moisés Hernandez‐Alonso Guadalupe Gonzalez‐Garcia Jose M. Mateos‐Rodriguez Miguel Fernandez‐Bermejo Javier P. Gisbert | 2012 | Helicobacter2012，，4： | 4 |
| 5 | Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations显示文摘AIM To compare the genomic variability and the multiple colonization of Helicobacter pylori(H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer(GC): Túquerres-Nari?o(High risk) and Tumaco-Nari?o(Low risk).METHODS Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa,(31/206) of the high risk population of GC(Túquerres) and(41/203) of the low risk population of GC(Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses. RESULTS The proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversityamong isolates of Túquerres(0.13) than those of Tumaco(0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco(P = 0.05).CONCLUSION In Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC. | Andrés Jenuer Matta Alvaro Jairo Pazos Javier Andrés Bustamante-Rengifo Luis Eduardo Bravo | 2017 | World Journal of Gastroenterology2017，23，5： | 2 |
| 6 | Volatile profile of Atlantic shellfish species by HS-SPME GC/MS显示文摘 | Glenda Fratini Salomé Lois Manuel Pazos Giuliana Parisi Isabel Medina | 2012 | Food Research International2012，，2： | 2 |
| 7 | In silico two-hybrid system for the selection of physically interacting protein pairs 显示文摘 | Pazos F Valencia A | 2002 | Proteins2002，47，2： | 1 |
| 8 | Do public subsidies stimulate private R&D spending?显示文摘 | ALEZ X G PAZO C | 2008 | Research Policy2008，，37： | 1 |
| 9 | Losartan titration versus diuretic combination in type 2 diabetics patients 显示文摘 | de Pablos-Velasco PL Pazos Toral F Esmatjes JE etal | 2002 | J Hypertens2002，20，4： | 1 |
| 10 | Proteomic evalu- ation of myofibrillar carbonylation in chilled fish mince and its inhibi- tion by catechin显示文摘 | PAZOS M MAESTRE R GALLARDO J M | 2013 | Food Chemistry2013，136，： | 1 |
| 11 | AVATAR: an improved solution for personalized TV based on semantic inference 显示文摘 | Fernandez Y B Pazos Arias J J Nores M L | 2006 | Consumer Electronics IEEE Transactions on2006，52，1： | 1 |
| 12 | Probabilistic load flow including wind power generation显示文摘 | VILLANUEVA D PAZOS J L FEIJOO A | 2011 | IEEE Transactions on Power Systems2011，26，3： | 1 |
| 13 | Liquid liquid extraction of lactic acid with alamine-336显示文摘 | MARTIN M S PAZOS C COCA J | 1996 | J Chem Tech Biotchnol1996，65，： | 1 |
| 14 | Stimulation of extracellular signal-regulated kinases and proliferation in the human gastric cancer cells KATO-Ⅲ by obestatin显示文摘 | Pazos Y Alvarez C J Camia J P Casanueva F F | 2007 | Growth Factors2007，25，6： | 1 |
| 15 | Similarity of phylogenetic trees as indicator of protein-protein interaction 显示文摘 | Pazos F Valencia A | 2001 | Protein Engineering2001，14，9： | 1 |
| 16 | Kinetic Analysis of the Interaction between Yessotoxin and Analogues and Immobilized Phosphodiesterases Using a Resonant Mirror Optical Biosensor显示文摘 | Pazos M J Alfonso A Vieytes M R | 2005 | Chem Res Toxicol2005，18，： | 1 |
| 17 | Destabilization of cutting oil emulsions using inorganic salts as coagulants显示文摘 | Rios G Pazos C Coca J 1998 | 1998 | Colloids and Surfaces A:Physicochemical and Engineering Aspects1998，138，： | 1 |
| 18 | Reproductive cycle of Manila clam Ruditapes philippinarum (Adams and Reeve 1850) ,in Ria of Vigo ( NW Spain ) 显示文摘 | Rodriguez M E Pazo J P Cortes F F | 1992 | Scientian Marina1992，56，1： | 1 |
| 19 | Acute renal failure following massive mannitol infusion 显示文摘 | P6rez-P6rez AJ Pazos B Sobrado J | 2002 | Am J Nephrol2002，22，56： | 1 |
| 20 | Volatile profile of Atlantic shellfish species by HS- SPME GC/MS 显示文摘 | Fratini G Lois S Pazos M | 2012 | Food Research International2012，48，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员