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388篇 您的检索式:期刊名="Protein Engineering"
    题名 作者 年代 出处 被引量
1Influence of size and polarity of rsidue 31 in porcine pancreatic-phospholipase A2 on catalytic properties显示文摘Kuipers OP Kerver J Van Meerohergen J 1990Protein Engineering 19901990,599,:1
2Investigations on the thermostability and function of trtmeated Thermus aquatieus DNA polymerase fragments 显示文摘ViUbrandt B Sagner G Sehomburg D 1997Protein Engineering1997,10,11:1
3An Escherichia-coli expression system that allow high-level soluble production of helerologous proteins显示文摘l avallie E Diblasio E Kovacic S 1993Protein Engineering1993,,:1
4Improvement of H202 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase显示文摘Miyazaki-Imamura C Oohira K Kitagawa R 2003Protein Engineering2003,16,6:1
5Site-directed mutagenesis and X-ray crystallography of two phosppholipase A2 mutants;Y52F and Y73F显示文摘Thunnissen MM Franhen PA Dsenth J 1992protein engineering1992,5,7:1
6Correlation be- tween stability of a protein and its dipeptide eomposition:A novel approach for predicting in vivo stability of a pro- tein from its primary sequence 显示文摘Guruprasad K Reddy B V Pandit M W 1990Protein Engineering1990,4,2:1
7Similarity of phylogenetic trees as indicator of protein-protein interaction 显示文摘Pazos F Valencia A 2001Protein Engineering2001,14,9:1
8Structure-activity studies of human tumor necrosis factor显示文摘Ostade X Tavernier J Fiers W 1994Protein Engineering1994,7,:1
9PCR - based gene synthesis as an efficient approach for expression of the A + T - rich malaria genome 显示文摘Chrislaine W M Elisabeth P C Fiona H 1999Protein Engineering1999,12,12:1
10Phage-displayed peptides selected for bind- ing to Campylobacter jejuni are antimicrobi- al显示文摘Bishop-Hurley SL Rea PL McSweeney CS 2010Protein Engineering Design & Selec- tion2010,23,10:1
11Thermostabilization of an esterase by alignment-guided focused directed evolution显示文摘Jochens H Aerts D Bornscheuer U T 2010Protein Engineering Design and Selection2010,23,12:1
12Improving protein solubility through rationally designed amino aeid replacemcnts: solubilization of the trimethoprim-resistant type S1 dihydrofolate reductase显示文摘Dale G E Broger C Langen H 1994Protein Engineering Design and Selection1994,,7:1
13Structure - function analysis of a conserved aromatic cluster in the N - terminal domain of human epidermal growth factor 显示文摘MARY B M DOUGLAS K TADAKI S R 1998Protein Engineering1998,11,11:1
14Directing phage selection towards specific epitopes显示文摘Parsons HL Earnshaw JC Wilton J 1996Protein Engineering1996,9,11:1
15Protein secondary structure predic- tion based on an improved support vector machines approach 显示文摘Hyunsoo Kim Haesun Park 2003Protein Engineering2003,16,8:1
16Computational modeling of type Ⅰ collagen fibers to determine the extracellular matrix structure of connective tissues显示文摘Meir Israelowitz 2005Protein Engineering Design &selection2005,18,7:1
17Structural and Energetic Determinants for Enantiopreferences in Kinetic Resolution of Lipases 显示文摘Bocola M Stubbs M T Sotriffer C 2003Protein Engineering2003,16,5:1
18The alpha/beta hydrolase fold显示文摘Ollis DL Cheah E Cygler M 1992Protein Engineering1992,5,:1
19Prion protein expression in Chinese hamster ovary cells using a glutamine synthetase selection and amplification system显示文摘Thomas C Blochberger Carol Cooper 1997Protein Engineering1997,10,12:1
20Identification of prokaryotic and eukaryotie signal peptides and prediction of their cleavage sites 显示文摘NIELSEN H ENGELBREEHT J BRUNAK S 1997Protein Engineering1997,10,1:1
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