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| 1 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 2 | Calcite veins of the Stfipa granite (Sweden) as records of the origin of the groundwaters and their interactions with the granitic body显示文摘 | NORBERT C SHAUN K F BERTRAND F | 1989 | Geochimica et Cosmochimica Acta1989，53，8： | 1 |
| 3 | Degradable biomaterials based on Magnesium corrosion 显示文摘 | Frank W Norbert H Carla V Smadar C Karl U K Regine W Frank F | 2008 | Current Opinion in Solid State and Materials Science2008，12，56： | 1 |
| 4 | Spontane- ous breathing during ventilatory support improves ventilation- perfusion distributions in patients with acute respiratory dis- tress syndrome显示文摘 | Putensen C Norbert JM Putensen-Himmer G | 1999 | Am J Respir Crit Care Med1999，159，： | 1 |
| 5 | Position control of linear switched reluctance motors for high -precision applications显示文摘 | Norbert C Cheung Li Qiu | 2003 | IEEE Transactions on Industry Applications2003，39，5： | 1 |
| 6 | Improved Grid Synchronization Control of Doubly Fed Induction Generator Under Unbalanced Grid Voltage显示文摘 | Sizhe Chen Norbert C Cheung Yun Zhang | 2011 | IEEE Transactions on Energy Conver- sion2011，26，3： | 1 |
| 7 | A Self-Tuning Regulator for the High-Precision Position Control of a Linear Switched Reluctance Motor 显示文摘 | Shi Wei Zhao Cheung Norbert C etc | 2007 | IEEE Transactions on Industrial Electronics2007，54，5： | 1 |
| 8 | Development of an instrument to electronically measure, record and image washover of a towed body显示文摘 | Norbert E Y James H C | 2003 | Applied Ocean Research2003，，25： | 1 |
| 9 | A Robust and Low-Cost Linear Motion System for Precision Manufacturing Automation显示文摘 | Norbert C Cheung | 2000 | IEEE Transactions on control systems technology2000，，： | 1 |
| 10 | High-throughput RNAi screening in cultured cells: a user's guide显示文摘 | Christophe C J Norbert N | 2006 | Nat Rev Genet2006，7，5： | 1 |
| 11 | Differential mobilization of P in the maize rhizosphere by citric acid and potassium citrate 显示文摘 | Laura P Norbert C Jones D L | 2006 | Soil Biology & Biochemistry2006，38，： | 1 |
| 12 | Im- proved grid synchronization control of doubly fed induc- tion generator under unbalanced grid voltage显示文摘 | Chen Si Zhe Cheung Norbert C Zhang Yun | 2011 | IEEE Trans on Energy Conversion2011，26，3： | 1 |
| 13 | Neuropsychological characterization of hepatic encephalopathy显示文摘 | Karin Weissenborn Jochen C Ennen Hans Schomerus Norbert Rückert Hartmut Hecker | 2001 | Journal of Hepatology2001，，5： | 1 |
| 14 | Spontaneous breathing during ventilatory support improves ventilation-perfusion distributions in patients with acute respiratory distress syndrome显示文摘 | Putensen C Norbert J M Putensen-Himmer G | 1999 | Am J Respir Crit Care Med1999，159，： | 1 |
| 15 | Use ofheadspace solid-phase microextraction and headspace sorptive extrac-tion for the detection of the volatile metabolites produced by toxigenicFusarium species显示文摘 | Demyttenaere J C R Morina R M Norbert De Kimpe | 2004 | J Chromatoff- A2004，1027，： | 1 |
| 16 | AT-CHLORO,A comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins显示文摘 | MYRIAM F SABINE B DANIEL S DAPHNE S B MAGALI C LUCAS M CLAIRE R STEPHANE M MOURAD M SOPHIE L G SYLVIE K J CHRISTOPHE B JEROME G JACQUES J CHRISTOPHE B JEROME G JACQUES J CHRISTOPHE M NORBERT R | 2010 | Molecular and Cellular Proteomics2010，9，： | 1 |
| 17 | Arterial and Venous Blood Flow: Noninvasive Quantitation with MR Imaging显示文摘 | Lorie R Norbert J Stephen C | 1992 | Radiology1992，185，3： | 1 |
| 18 | Rb-Sr and K-Ar dating of clay diagenesis in Jurassic Sandstone Oil Reservoir, North Sea显示文摘 | NICOLE L NORBERT C FREDERIC S | 1987 | AAPG Bull1987，71，12： | 1 |
| 19 | Etheno adducts formed in DNA of vinyl chloride-exposed rats are highly persistent in liver 显示文摘 | Swenberg J Norbert F Francoise C | 1992 | Carcinogenesis1992，13，4： | 1 |
| 20 | Acute Leukemia during Pregnancy:A Report on 37 patients and a review of the literature显示文摘 | Youcef C Norbert V Emmanuel R | 2005 | Int J Gynecol Cancer2005，104，： | 1 |
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