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| 1 | Obese diet-induced mouse models of nonalcoholic steatohepatitis-tracking disease by liver biopsy显示文摘AIM:To characterize development of diet-induced nonalcoholic steatohepatitis(NASH)by performing live biopsy in wild-type and genetically obese mice.METHODS:Male wild-type C57BL/6J(C57)mice(DIO NASH)and male Lep ob/Lep ob(ob/ob)mice(ob/ob-NASH were maintained on a diet high in trans-fat(40%)fructose(22%)and cholesterol(2%)for 26 and 12 wk respectively.A normal chow diet served as control in C57 mice(lean chow)and ob/ob mice(ob/ob chow)After the diet-induction period,mice were liver biopsied and a blinded histological assessment of steatosis and fibrosis was conducted.Mice were then stratified into groups counterbalanced for steatosis score and fibrosi stage and continued on diet and to receive daily PO dosing of vehicle for 8 wk.Global gene expression in liver tissue was assessed by RNA sequencing and bioin formatics.Metabolic parameters,plasma liver enzyme and lipids(total cholesterol,triglycerides)as well a hepatic lipids and collagen content were measured b biochemical analysis.Non-alcoholic fatty liver disease activity score(NAS)(steatosis/inflammation/ballooningdegeneration)and fibrosis were scored.Steatosis and fibrosis were also quantified using percent fractional area.RESULTS:Diet-induction for 26 and 12 wk in DIONASH and ob/ob-NASH mice,respectively,elicited progressive metabolic perturbations characterized by increased adiposity,total cholesterol and elevated plasma liver enzymes.The diet also induced clear histological features of NASH including hepatosteatosis and fibrosis.Overall,the metabolic NASH phenotype was more pronounced in ob/ob-NASH vs DIO-NASH mice.During the eight week repeated vehicle dosing period,the metabolic phenotype was sustained in DIO-NASH and ob/ob-NASH mice in conjunction with hepatomegaly and increased hepatic lipids and collagen accumulation.Histopathological scoring demonstrated significantly increased NAS of DIO-NASH mice(0 vs4.7±0.4,P<0.001 compared to lean chow)and ob/ob-NASH mice(2.4±0.3 vs 6.3±0.2,P<0.001compared to ob/ob chow),respectively.Furthermore,fibrosis stage was significantly elevated for DIO-NASH mice(0 vs 1.2±0.2,P<0.05 compared to lean chow)and ob/ob NASH(0.1±0.1 vs 3.0±0.2,P<0.001compared to ob/ob chow).Notably,fibrosis stage was significantly(P<0.001)increased in ob/ob-NASH mice,when compared to DIO-NASH mice.CONCLUSION:These data introduce the obese dietinduced DIO-NASH and ob/ob-NASH mouse models with biopsy-confirmed individual disease staging as a preclinical platform for evaluation of novel NASH therapeutics. | Maria Nicoline Baandrup Kristiansen Sanne Skovgard Veidal Kristoffer Tobias Gustav Rigbolt Kirstine Sloth Tolbol Jonathan David Roth Jacob Jelsing Niels Vrang Michael Feigh | 2016 | World Journal of Hepatology2016，8，16： | 10 |
| 2 | Study on the particle breakage of ballast based on a GPU accelerated discrete element method显示文摘Breakage of particles will have greatly influence on mechanical behavior of granular material(GM)under external loads,such as ballast,rockfill and sand.The discrete element method(DEM)is one of the most popular methods for simulating GM as each particle is represented on its own.To study breakage mechanism of particle breakage,a cohesive contact mode is developed based on the GPU accelerated DEM code-Blaze-DEM.A database of the 3D geometry model of rock blocks is established based on the 3D scanning method.And an agglomerate describing the rock block with a series of non-overlapping spherical particles is used to build the DEM numerical model of a railway ballast sample,which is used to the DEM oedometric test to study the particles’breakage characteristics of the sample under external load.Furthermore,to obtain the meso-mechanical parameters used in DEM,a black-analysis method is used based on the laboratory tests of the rock sample.Based on the DEM numerical tests,the particle breakage process and mechanisms of the railway ballast are studied.All results show that the developed code can better used for large scale simulation of the particle breakage analysis of granular material. | Guang-Yu Liu Wen-Jie Xu Qi-Cheng Sun Nicolin Govender | 2020 | Geoscience Frontiers2020，11，2： | 3 |
| 3 | Screening for refractive errors in children: The plusoptiX S08 and the Retinomax K-plus2 performed by a lay screener compared to cycloplegic retinoscopy显示文摘 | Tamara Paff Anne Marie Oudesluys-Murphy Ron Wolterbeek Marietta Swart-van den Berg Johan M. de Nie Els Tijssen Nicoline E. Schalij-Delfos | 2010 | Journal of AAPOS2010，，6： | 1 |
| 4 | Breast adenocarcinoma MCF-7 cell line induces spontaneous osteoclastogenesis via a RANK-ligand-dependent pathway 显示文摘 | Nicolin V Bortul R Bareggi R | 2008 | Acta Histochem2008，110，5： | 1 |
| 5 | Antitumor immunity induced by photodynamic therapy with aluminum disulfonated phthalocyanines and laser light显示文摘 | Canti G Lattuada D Nicolin A | 1994 | Anticancer Drugs1994，5，： | 1 |
| 6 | Antitumor immunity induced by photodynamic therapy with aluminum disulfonated phthalocyanines and laser light 显示文摘 | Canti G Lattuada D Nicolin A | 1994 | Anticancer Drugs1994，5，： | 1 |
| 7 | Impact of Platelet Reactivity on Clinical Outcomes After Percutaneous Coronary Intervention显示文摘 | Somjot S. Brar Jurrien ten Berg Rossella Marcucci Matthew J. Price Marco Valgimigli Hyo-Soo Kim Giuseppe Patti Nicoline J. Breet Germano DiSciascio Thomas Cuisset George Dangas | 2011 | Journal of the American College of Cardiology2011，，19： | 1 |
| 8 | Poor prognosis of constitutive γ-H2AX expressing triple-negative breast cancers is associated with telomere length显示文摘 | Anika Nagelkerke Simon JA van Kuijk John W Martens Fred CGJ Sweep Nicoline Hoogerbrugge Johan Bussink Paul N Span | 2015 | Biomark. Med2015，，4： | 1 |
| 9 | Candidate colorectal cancer predisposing gene variants in Chinese early-onset and familial cases显示文摘AIM: To investigate whether whole-exome sequencing may serve as an efficient method to identify known or novel colorectal cancer(CRC) predisposing genes in early-onset or familial CRC cases.METHODS: We performed whole-exome sequencing in 23 Chinese patients from 21 families with nonpolyposis CRC diagnosed at ≤ 40 years of age, or from multiple affected CRC families with at least 1 firstdegree relative diagnosed with CRC at ≤ 55 years of age.Genomic DNA from blood was enriched for exome sequences using the Sure Select Human All Exon Kit, version 2(Agilent Technologies) and sequencing was performed on an Illumina Hi Seq 2000 platform.Data were processed through an analytical pipeline to search for rare germline variants in known or novel CRC predisposing genes.RESULTS: In total, 32 germline variants in 23 genes were identified and confirmed by Sanger sequencing.In 6 of the 21 families(29%), we identified 7 mutations in 3 known CRC predisposing genes including MLH1(5 patients), MSH2(1 patient), and MUTYH(biallelic, 1 patient), five of which were reported as pathogenic.Inthe remaining 15 families, we identified 20 rare and novel potentially deleterious variants in 19 genes, six of which were truncating mutations.One previously unreported variant identified in a conserved region of EIF2AK4(p.Glu738_Asp739insA rgA rg) was found to represent a local Chinese variant, which was significantly enriched in our early-onset CRC patient cohort compared to a control cohort of 100 healthy Chinese individuals scored negative by colonoscopy(33.3% vs 7%, P < 0.001).CONCLUSION: Whole-exome sequencing of early-onset or familial CRC cases serves as an efficient method to identify known and potential pathogenic variants in established and novel candidate CRC predisposing genes. | Jun-Xiao Zhang Lei Fu Richarda M de Voer Marc-Manuel Hahn Peng Jin Chen-Xi Lv Eugène TP Verwiel Marjolijn JL Ligtenberg Nicoline Hoogerbrugge Roland P Kuiper Jian-Qiu Sheng Ad Geurts van Kessel | 2015 | World Journal of Gastroenterology2015，21，14： | 1 |
| 10 | mTOR: a protein kinase switching between life and death显示文摘 | Laura Asnaghi Paola Bruno Marcella Priulla Angelo Nicolin | 2004 | Pharmacological Research2004，，6： | 1 |
| 11 | The efficacy of black tea in ameliorating endothelial function is equivalent to that of green tea显示文摘 | Nicoline Jochmann Mario Lorenz Ame lie von Krosigk | 2008 | British Journal of Nutrition2008，，99： | 1 |
| 12 | Natural history and outcome of optic pathway gliomas in children 显示文摘 | Nicolin G Parkin P Mabbott D | 2009 | Pediatr Blood Cancer2009，53，7： | 1 |
| 13 | Breast adenocarcinomaMCF-7 cell line induces spontaneous osteoclastogenesis via aRANK-ligand-dependent pathway 显示文摘 | Nicolin V Bortul R Bareggi R | 2008 | Acta Histoehem2008，110，5： | 1 |
| 14 | Thermodynamic and economic optimization of a MCFC-based hybrid system for the combined production of electricity and hydrogen显示文摘 | Verda V Nicolin F | 2010 | International Journal of Hydrogen Energy2010，35，2： | 1 |
| 15 | Effect of tanshinone II on cell growth of breast cancer cell line type MCF-7 and MDMB-231显示文摘 | Nicolin V Fancellu G Valentini R | 2014 | Italian Journal of Anatomy and Embryology2014，119，1： | 1 |
| 16 | Lifetime optimization of a molten carbonate fuel cell power system coupled with hydrogen production 显示文摘 | NICOLIN F VEREIJA V | 2011 | Energy2011，36，： | 1 |
| 17 | Female-specific aspects in the pharmacotherapy of chronic cardiovascular diseases显示文摘 | Nicoline J Karl S Edeltraut G | 2005 | Eur Heart J2005，26，： | 1 |
| 18 | Thermodynamic and economic optimization of a MCFC-based hybrid system for the combined production of electricity and hydrogen显示文摘 | VERDA V NICOLIN F | 2010 | Int J Hydrogen Energy2010，35，： | 1 |
| 19 | mTOR:A protein ld- nase switching between life and death显示文摘 | Asnaghi L Bruno P Priulla M Nicolin A | 2004 | Phamacol Res2004，50，8： | 1 |
| 20 | Receptor activator for nuclear factor kappa B ligand (RANKL) as an osteoimmune key regulator in bone physiology and pathology显示文摘 | NARDUCCI P BAREGGI R NICOLIN V | 2009 | Acta Histochem2009，10，： | 1 |
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