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| 1 | Loss of the vitamin D receptor in human breast and prostate cancers strongly induces cell apoptosis through downregulation of Wnt/β-catenin signaling显示文摘Vitamin D co-regulates cell proliferation, differentiation and apoptosis in numerous tissues, including cancers. The known anti-proliferative and pro-apoptotic actions of the active metabolite of vitamin D,1,25-dihydroxy-vitamin D [1,25(OH)2 D] are mediated through binding to the vitamin D receptor(VDR). Here,we report on the unexpected finding that stable knockdown of VDR expression in the human breast and prostate cancer cell lines, MDA-MB-231 and PC3, strongly induces cell apoptosis and inhibits cell proliferation in vitro. Implantation of these VDR knockdown cells into the mammary fat pad(MDA-MB-231),subcutaneously(PC3) or intra-tibially(both cell lines) in immune-incompetent nude mice resulted in reduced tumor growth associated with increased apoptosis and reduced cell proliferation compared with controls.These growth-retarding effects of VDR knockdown occur in the presence and absence of vitamin D and are independent of whether cells were grown in bone or soft tissues. Transcriptome analysis of VDR knockdown and non-target control cell lines demonstrated that loss of the VDR was associated with significant attenuation in the Wnt/β-catenin signaling pathway. In particular, cytoplasmic and nuclear β-catenin protein levels were reduced with a corresponding downregulation of downstream genes such as Axin2, Cyclin D1,interleukin-6(IL-6), and IL-8. Stabilization of β-catenin using the GSK-3β inhibitor BIO partly reversed the growth-retarding effects of VDR knockdown. Our results indicate that the unliganded VDR possesses hitherto unknown functions to promote breast and prostate cancer growth, which appear to be operational not only within but also outside the bone environment. These novel functions contrast with the known anti-proliferative nuclear actions of the liganded VDR and may represent targets for new diagnostic and therapeutic approaches in breast and prostate cancer. | Yu Zheng Trupti Trivedi Ruby CY Lin Colette Fong-Yee Rick Nolte Jeline Manibo Yunzhao Chen Musharraf Hossain Konstantin Horas Colin Dunstan Hong Zhou Markus J Seibel | 2017 | Bone Research2017，5，3： | 4 |
| 2 | Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities显示文摘 | Ilkay Erdogan Orhan Bilge ?ener Syed Ghulam Musharraf | 2010 | Experimental and Toxicologic Pathology2010，，3： | 2 |
| 3 | Photodegradation of norfloxacin in aqueous and organic solvents: a kinetic study显示文摘 | AHMAD I BANO R MUSHARRAF S G | 2015 | J Photoch Photobio A2015，302，： | 1 |
| 4 | Biotransformation of an antimalarial drug, artemether by plant and fungal cell cultures显示文摘 | Musharraf S G Uddin J Akhter M | 2012 | J Mol Catal B Enzym2012，82，12： | 1 |
| 5 | Resilient Moduli of Granular Materials 显示文摘 | Musharraf Z Dar-Hao Chen Joakim Laguros | 1995 | Journal of Transportation Engineering1995，120，6： | 1 |
| 6 | lnfluence of Recycled Asphalt Pavement on Creep Compliance of Hot Mix Asphalt显示文摘 | Pranshoo Solanki Dsvid Adje Musharraf Zaman Zahid Hossain | 2012 | Springer Series in Geomechanics & Geoengineering Constitutive Modeling of Geomaterials Advances and New Applications2012，，10： | 1 |
| 7 | Calibration of non-nuclear PQI gau- ges and field comparison of PQI and nuclear gauge densities显示文摘 | Wasiuddin Nazimuddin M Zaman Musharraf M Commuri Sesh | 2009 | International Journal of Pavement Re- search and Technology2009，2，5： | 1 |
| 8 | Field and laboratory properties of lime-treated cold In-place recycled asphalt pavements 显示文摘 | RAFICQUL ALAM TAREFDER MUSHARRAF ZAMAN ROLAND SISSON | 2006 | Journal of Testing and Evaluation2006，34，1： | 1 |
| 9 | Com- parison of plasma from healthy nonsmokers ,smokers, and lung cancer patients : pattern-based differentiation profiling of low molecular weight proteins and peptides by magnetic bead technology with MALDI- TOF-MS 显示文摘 | Syed G Musharraf Naghma Hashmi Iqbal Choudhary M | 2012 | Biomarkers2012，17，3： | 1 |
| 10 | Dynamic response of cable-stayed bridges to moving ve- hicles using the structural impedance method 显示文摘 | MUSHARRAF Z MICHEAL R T ANURADHA K | 1996 | Applied Mathematical Modelling1996，20，12： | 1 |
| 11 | STABILITY OF BOREHOLES IN A GEOLOGIC MEDIUM INCLUDING THE EFFECTS OF ANISOTROPY显示文摘IntroductionThestudyofresponseofaboreholetochangingloadingconditionsisofparticularinteresttoeveralengineringfieldsincludingpe... | Dinesh Gupta, Musharraf Zaman (School of Civil Engineering and Environmental Science, University of Oklahoma, Norman, OK 73019, U S A) (Communicated by Chien Weizang) | 1999 | Applied Mathematics and Mechanics(English Edition)1999，20，8： | 1 |
| 12 | Isolation of bioactive compounds from Aspergillus terreus显示文摘 | Choudhary M I Musharraf S G Mukhmoor T | 2004 | Z Naturforsch B2004，59，3： | 1 |
| 13 | Biodiesel production from microalgal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis显示文摘 | Syed Ghulam Musharraf Muhammad Arif Ahmed Noureen Zehra Nurul Kabir M Iqbal Choudhary Atta-ur Rahman | 2012 | Chemistry Central Journal2012，，1： | 1 |
| 14 | Human reliability assessment during offshore emergency conditions显示文摘 | MUSHARRAF M HASSAN J KHAN F | 2013 | Safety Science2013，59，： | 1 |
| 15 | Analysis of Emulsion and Hot Asphalt Cement Chip Seal Performance显示文摘 | Douglas D Gransberg Musharraf Zaman | 2005 | Jour- nal of Transportation Engineering(ASCE)2005，131，3： | 1 |
| 16 | Plasmid-mediated multiple antibiotic resistance in Proteus mirabilis isolated from patients with urinary tract infection显示文摘 | Khan A Musharraf A | 2004 | Med Sci Monit2004，10，11： | 1 |
| 17 | Plasmid - mediated muhiple antibiotic resistance in Proteus mirabilis isolated from patients with urinary tract infection显示文摘 | Khan A Musharraf A | 2004 | Med Sci Monit2004，10，11： | 1 |
| 18 | Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities显示文摘 | Orhan I E Sener B Musharraf S G | 2012 | Exp Toxicol Pathol2012，64，： | 1 |
| 19 | Microbial transformation of antifertility agents, norethisterone and 17α-ethynylestradiol显示文摘 | Choudhary MI Musharraf SG Ali RA | 2004 | Zeitschrift fur Naturforschung2004，59，： | 1 |
| 20 | Humanreliability assessment during offshore emergency conditions显示文摘 | MUSHARRAF M HASSAN J KHAN F | 2013 | Safety Science2013，，59： | 1 |
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