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| 1 | Epidemiological characteristics and temporal-spatial analysis of overseas imported dengue fever cases in outbreak provinces of China, 2005–2019显示文摘Background Overseas imported dengue fever is an important factor in local outbreaks of this disease in the mainland of China.To better prevent and control such local outbreaks,the epidemiological characteristics and temporal-spatial distribution of overseas imported dengue fever cases in provincial-level administrative divisions(PLADs)where dengue fever is outbreak in the mainland of China were explored.Methods Using the Chinese National Notifiable Infectious Disease Reporting Information System(CNNDS),we identified overseas imported dengue fever cases in dengue fever outbreak areas in the mainland of China from 2005 to 2019 to draw the epidemic curve and population characteristic distribution of overseas imported cases in each PLAD.Based on spatial autocorrelation analysis of ArcGIS 10.5 and temporal-spatial scanning analysis of SaTScan 9.5,we analyzed the temporal-spatial distribution of overseas imported dengue fever in dengue fever outbreak areas in the mainland of China.Results A total of 11,407 imported cases,mainly from Southeast Asia,were recorded from 2005 to 2019 in these 13 PLADs.Of which 62.1%were imported into Yunnan and Guangdong Provinces.Among the imported cases,there were more males than females,mainly from the 21–50 age group.The hot spots were concentrated in parts of Yunnan,Guangdong and Fujian Provinces.We found the cluster of infected areas were expanding northward.Conclusions Based on the analysis of overseas imported dengue cases in 13 PLADs of the mainland of China from 2005 to 2019,we obtained the epidemiological characteristics and spatial distribution of imported dengue cases.Border controls need to pay attention to key population sectors,such as 21–50 years old men and education of key populations on dengue prevention.There is a need to improve the awareness of the prevention and control of imported cases in border areas.At the same time,northern regions cannot relax their vigilance. | Xinchang Lun Yiguan Wang Chunchun Zhao Haixia Wu Caiying Zhu Delong Ma Mingfang Xu Jun Wang Qiyong Liu Lei Xu Fengxia Meng | 2022 | Infectious Diseases of Poverty2022，11，1： | 3 |
| 2 | Long-term selection for resistance to transgenic cotton expressing Bacillus thuringiensis toxin in Helicoverpa armigera ( Hubner ) ( Lepidoptera : Noctuidae) 显示文摘 | Meng Fengxia Shen Jinliang Zhou Weijun | 2004 | Pest Manag Sci2004，60，2： | 1 |
| 3 | Establishment and Application of Heminested RT-PCR Assay for Detection of Mosquito-Borne Flavivirus—Guizhou Province,China,2018显示文摘Introduction:Mosquito-borne flavivirus can lead to serious infectious diseases worldwide and cause high mortality and disability.In order to strengthen epidemiological investigation of flavivirus and meet the needs of early warning of diseases,a simple,rapid,and sensitive detection method needs to be established to prevent and control mosquito-borne diseases.Methods:Using the NS5 gene of flavivirus in GenBank,3 universal primers targeting the conserved regions were designed.The complementary DNAs(cDNAs)of Japanese encephalitis virus(JEV),dengue virus(DENV),West Nile virus(WNV),and yellow fever virus(YFV)were used as the template to optimize the reaction conditions.A heminested reversetranscriptase polymerase chain reaction(hnRT-PCR)was established to verify the sensitivity and specificity of this method and to detect field-caught mosquitoes.Results:Our results showed that this method exhibited better specificity,higher sensitivity,and the ability to detect multiple viruses simultaneously.The lowest detection limit of JEV,DENV-2,YFV,and WNV was 3×10^(4),3×10^(6),3×10^(5),and 3×10^(4)copies/μL,respectively.Mosquito-borne flavivirus was successfully detected in the field-caught mosquito samples using the method established in this study.Discussion:The hnRT-PCR method established in this study can be employed for the rapid detection of flavivirus and provide technical support for early and rapid diagnosis of mosquito-borne flavivirus. | Zhijing Xue Ning Zhao Jun Wang Xiuping Song Fengxia Meng Wenqin Liang Jingzhu Zhou Dan Wang Zhong Zhang Qiyong Liu | 2021 | China CDC weekly2021，3，1： | 1 |
| 4 | Regulation of nitrogen starvation responses by the alarmone(p)ppGpp in rice显示文摘Nitrogen is an essential macronutrient for all living organisms and is critical for crop productivity and quality.In higher plants,inorganic nitrogen is absorbed through roots and then assimilated into amino acids by the highly conserved glutamine synthetase/glutamine:2-oxoglutarate aminotransferase(GS/GOGAT)cycle.How nitrogen metabolism and nitrogen starvation responses of plants are regulated remains largely unknown.Previous studies revealed that mutations in the rice ABNORMAL CYTOKININ RESPONSE1(ABC1)gene encoding Fd-GOGAT cause a typical nitrogen deficiency syndrome.Here,we show that ARE2(for ABC1 REPRESSOR2)is a key regulator of nitrogen starvation responses in rice.The are2 mutations partially rescue the nitrogen-deficient phenotype of abc1 and the are2 mutants show enhanced tolerance to nitrogen deficiency,suggesting that ARE2 genetically interacts with ABC1/Fd-GOGAT.ARE2 encodes a chloroplast-localized Rel A/Spo T homolog protein that catalyzes the hydrolysis of guanosine pentaphosphate or tetraphosphate(p)pp Gpp,an alarmone regulating the stringent response in bacteria under nutritional stress conditions.The are2 mutants accumulate excessive amounts of(p)pp Gpp,which correlate with lower levels of photosynthetic proteins and higher amino acid levels.Collectively,these observations suggest that the alarmone(p)pp Gpp mediates nitrogen stress responses and may constitute a highly conserved mechanism from bacteria to plants. | Hanwen Li Jinqiang Nian Shuang Fang Meng Guo Xiahe Huang Fengxia Zhang Qing Wang Jian Zhang Jiaoteng Bai Guojun Dong Peiyong Xin Xianzhi Xie Fan Chen Guodong Wang Yingchun Wang Qian Qian Jianru Zuo Jinfang Chu Xiaohui Ma | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 5 | A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology显示文摘Background The current prevention and control strategy forAedes albopictus heavily relies on comprehensive management, such as environmental management and chemical control. However, the wide application of pyrethroids has facilitated the development of insecticide resistance, primarily via mutations in the voltage-gated sodium channel (VGSC) gene. This study aims to develop a novel strategy for detecting mutations in the VGSC gene inAe. albopictus using multiplex PCR-mass spectrometry (MPCR-MS) minisequencing technology.Methods We established a new strategy for detecting mutations in the VGSC gene inAe. albopictus using MPCR-MS minisequencing technology. MPCR amplification and mass probe extension (MPE) were first used, followed by single nucleotide polymorphism (SNP) typing mass spectrometry, which allows the simultaneous detection of multiple mutation sites of the VGSC gene in 96 samples ofAe. albopictus. A total of 70 wild-collectedAe. albopictus were used to evaluate the performance of the method by comparing it with other methods.Results Three target sites (1016, 1532, 1534) in the VGSC gene can be detected simultaneously by double PCR amplification combined with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, achieving a detection limit of 20 fg/μl. We applied this method to 70 wild-collectedAe. albopictus, and the obtained genotypes were consistent with the routine sequencing results, suggesting the accuracy of our method.Conclusions MPCR-MS minisequencing technology provides a sensitive and high-throughput approach toAe. albopictus VGSC gene mutation screening. Compared with conventional sequencing, this method is economical and time-saving. It is of great value for insecticide resistance surveillance in areas with a high risk of vector-borne disease. | Qunzheng Mu Xin Zhao Fengfeng Li Wenyu Li Xinxin Zhou Xinchang Lun Yiguan Wang Dongdong Hua Qiyong Liu Di Xiao Fengxia Meng | 2023 | Infectious Diseases of Poverty2023，12，4： | 0 |
| 6 | Transgenerational analysis of H3K4me3 and H3K27me3 by ChIP-Seq links epigenetic inheritance to metabolism显示文摘Histone methylation is a kind of important epigenetic modification which occurs on the lysine residue or arginine residue of histone tails(Zhang and Reinberg,2001).It takes part in multiple biological processes,including gene expression,genomic stability,stem cell maturity,genetic imprinting,mitosis and development(Fischle et al.,2005).Abnormal histone methylation pattern | Ke An Fengxia Du Hao Meng Guochao Li Minjie Zhang Zongzhi Liu Zitong Zhao Zilong Zhang Di Yu Dong Wang Caiyun Yang Wencui Ma Lin Yuan Meiting Zhou Lili Duan Li Jin Hui Li Yan Zhang Jianzhong Su Jie Qiao Yingli Sun | 2018 | Journal of Genetics and Genomics2018，45，3： | 0 |
| 7 | Metagenomic analysis reveals oropharyngeal microbiota alterations in patients with COVID-19显示文摘COVID-19 remains a serious emerging global health problem,and little is known about the role of oropharynx commensal microbes in infection susceptibility and severity.Here,we present the oropharyngeal microbiota characteristics identified by shotgun metagenomic sequencing analyses of oropharynx swab specimens from 31 COVID-19 patients,29 influenza B patients,and 28 healthy controls.Our results revealed a distinct oropharyngeal microbiota composition in the COVID-19 patients,characterized by enrichment of opportunistic pathogens such as Veillonella and Megasphaera and depletion of Pseudopropionibacterium,Rothia,and Streptococcus.Based on the relative abundance of the oropharyngeal microbiome,we built a microbial classifier to distinguish COVID-19 patients from flu patients and healthy controls with an AUC of 0.889,in which Veillonella was identified as the most prominent biomarker for COVID-19 group.Several members of the genus Veillonella,especially Veillonella parvula which was highly enriched in the oropharynx of our COVID-19 patients,were also overrepresented in the BALF of COVID-19 patients,indicating that the oral cavity acts as a natural reservoir for pathogens to induce co-infections in the lungs of COVID-19 patients.We also found the increased ratios of Klebsiella sp.,Acinetobacter sp.,and Serratia sp.were correlated with both disease severity and elevated systemic inflammation markers(neutrophil-lymphocyte ratio,NLR),suggesting that these oropharynx microbiota alterations may impact COVID-19 severity by influencing the inflammatory response.Moreover,the oropharyngeal microbiome of COVID-19 patients exhibited a significant enrichment in amino acid metabolism and xenobiotic biodegradation and metabolism.In addition,all 26 drug classes of antimicrobial resistance genes were detected in the COVID-19 group,and were significantly enriched in critical cases.In conclusion,we found that oropharyngeal microbiota alterations and functional differences were associated with COVID-19 severity. | Shengli Ma Fan Zhang Fengxia Zhou Hui Li Wenyu Ge Rui Gan Huan Nie Biao Li Yindong Wang Meng Wu Duo Li Dongmei Wang Zheng Wang Yuhong You Zhiwei Huang | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 0 |
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