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| 1 | A Rare Allele of GS2 Enhances Grain Size anc Grain Yield in Rice显示文摘谷物尺寸决定谷物重量并且影响谷物质量。调整谷物尺寸的几主要量的特点 loci (QTL ) 被克隆;然而,我们调整米饭谷物的尺寸的内在的机制的理解仍然保持碎片。这里,我们报导克隆和主导的 QTL 的描述,染色体 2 上的谷物尺寸(GS2 ) 编码调整生长的因素 4,一个 transcriptional 管理者。GS2 本地化到原子核并且可以充当抄写使活跃之物。影响 microRNA 的有约束力的地点的 GS2 的一个稀罕变化, OsmiR396c,原因提高了 GS2/OsGRF4 的表示。GS2 表示的增加导致更大的房间和房间的增加的数字,它因此提高谷物重量和产量。进米饭栽培变种的 GS2/OsGRF4 的这稀罕等位基因的介绍能显著地提高谷物重量和增加谷物产量,用在引起产量很高的米饭变化的可能的应用。 | Jiang Hu Yuexing Wang Yunxia Fang Longjun Zeng Jie Xu Haiping Yu Zhenyuan Shi Jiangjie Pan Dong Zhang Shujing Kang Li Zhu Guojun Dong Longbiao Guo Dali Zeng Guangheng Zhang Lihong Xie Guosheng Xiong Jiayang Li Qian Qian | 2015 | Molecular Plant2015，8，10： | 57 |
| 2 | Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies显示文摘当通过夫酸安 synthetase/glutamine:2-oxoglutarate amidotransferase (GS/GOGAT ) 的 Gln 和 Glu 骑车,植物吸收从土壤吸收进器官的形式的无机的氮。而 GS 从 Glu 和氨催化 Gln 的形成, GOGAT 从 Gln 催化一个酰胺组的转移到 2-oxoglutarate 生产 Glu 的二个分子。然而,处于碳氮平衡的 GS/GOGAT 周期的规章的角色很好没被理解。这里,我们报导米饭的功能的描述反常细胞激动素反应 1 (ABC1 ) 编码一个铁氧化还原蛋白依赖者(Fd ) 的基因 -GOGAT。弱变异的等位基因 abc1-1 异种显示出典型氮缺乏的症候群,而 T-DNA insertional 异种 abc1-2 是幼苗致命。Metabolomics 分析在 abc1-1 在 tricarboxylic 酸周期与高 N/C 比率(Gln 和 Asn ) 和几中介揭示了氨基酸的过多的数量的累积,建议 ABC1 起在氮吸收和碳氮平衡的一个关键作用。五非同义的单个核苷酸的多型性在编码区域的 ABC1 被识别并且作为三不同 haplotypes 描绘了,它高度并且明确地在装饰用的梨树和 indica 亚种之间被区分了。一起,这些结果建议 ABC1/OsFd-GOGAT 由 modulating 氮吸收和碳氮平衡为植物生长和开发是必要的。 | Xiaolu Yang Jinqiang Nian Qincliun xie Jian Feng Fengxia Zhang Hongwei Jing Jian Zhang Guojun Dong Yan Liang Juli Peng GuodongWang Qian Qian Jianru Zuo | 2016 | Molecular Plant2016，9，11： | 14 |
| 3 | The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice显示文摘Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops. | Jianqin Hao Dekai Wang Yingbao Wu Ke Huang Penggen Duan Na Li Ran Xu Dali Zeng Guojun Dong Baolan Zhang Limin Zhang Dirk Inze Qian Qian Yunhai Li | 2021 | Molecular Plant2021，14，8： | 14 |
| 4 | ALK,the Key Gene for Gelatinization Temperature,is a Modifier Gene for Gel Consistency in Rice显示文摘Gelatinization temperature(GT) is an important parameter in evaluating the cooking and eating quality of rice.Indeed,the phenotype,biochemistry and inheritance of GT have been widely studied in recent times.Previous map-based cloning revealed that GT was controlled by ALK gene,which encodes a putative soluble starch synthase II-3.Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium.Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT.Meanwhile,amylose content,gel consistency and pasting properties were also affected in transgenic lines.Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT.Based on the single nucleotide polymorphisms(SNPs),two new sets of SNP markers combined with one cleaved amplified polymorphic sequence marker were developed for application in rice quality breeding. | Zhenyu Gao Dali Zeng Fangmin Cheng Zhixi Tian Longbiao Guo Yan Su Meixian Yan Hua Jiang Guojun Dong Yuchen Huang Bin Han Jiayang Li Qian Qian | 2011 | Journal of Integrative Plant Biology2011，53，9： | 13 |
| 5 | The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice显示文摘The genetic improvement of nitrogen use efficiency(NUE)of crops is vital for grain productivity and sustainable agriculture.However,the regulatory mechanism of NUE remains largely elusive.Here,we report that the rice Grain number,plant height,and heading date7(Ghd7)gene genetically acts upstream of ABC1 REPRESSOR1(ARE1),a negative regulator of NUE,to positively regulate nitrogen utilization.As a transcriptional repressor,Ghd7 directly binds to two Evening Element-like motifs in the promoter and intron 1 of ARE1,likely in a cooperative manner,to repress its expression.Ghd7 and ARE1 display diurnal expression patterns in an inverse oscillation manner,mirroring a regulatory scheme based on these two loci.Analysis of a panel of 2656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during breeding.Moreover,the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South Asia.Remarkably,the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions.Collectively,these results define a Ghd7–ARE1-based regulatory mechanism of nitrogen utilization,providing useful targets for genetic improvement of rice NUE. | Qing Wang Qingmei Su Jinqiang Nian Jian Zhang Meng Guo Guojun Dong Jiang Hu Rongsheng Wang Changshuo Wei Guanwen Li Wan Wang Hui-Shan Guo Shaoyang Lin Wenfeng Qian XianzhiXie Qian Qian Fan Chen Jianru Zuo | 2021 | Molecular Plant2021，14，6： | 10 |
| 6 | Xiaowei, a New Rice Germplasm for Large-Scale Indoor Research显示文摘Dear Editor,Rice (Oryza sativa)is a model monocot plant for biological studies due to its relatively small genome,rich germplasm resources,and high-efficiency transformation methods.Although significant progress has been made in rice genomics and functional genomics research (Li et al.,2018),large-scale indoor research toward better understanding of rice biology is hampered by its long growth period and extreme dependence on the natural environment for cultivation.There are some rice germplasms that have a short growth period and short plant height,such as Kitaake. | Shikai Hu Xingming Hu Jiang Hu Lianguang Shang Guojun Dong Dali Zeng Longbiao Guo Qian Qian | 2018 | Molecular Plant2018，11，11： | 10 |
| 7 | Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L.显示文摘An es-t (early senescence-temporary) mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identified in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffin section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromosome 5. These results will facilitate the positional cloning and functional studies of the gene. | YANG YaoLong RAO YuChun LIU HuiJuan FANG YunXia DONG GuoJun HUANG LiChao LENG YuJia GUO LonBiao ZHANG GuangHeng HU Jiang GAO ZhenYu QIAN Qian ZENG DaLi | 2011 | Chinese Science Bulletin2011，56，23： | 8 |
| 8 | Development of nutritious rice with high zinc/selenium and low cadmium in grains through QTL pyramiding显示文摘Enriching zinc(Zn) and selenium(Se) levels,while reducing cadmium(Cd) concentration in rice grains is of great benefit for human diet and health.Large natural variations in grain Zn, Se, and Cd concentrations in different rice accessions enable Zn/Sebiofortification and Cd-minimization through molecular breeding. Here, we report the development of new elite varieties by pyramiding major quantitative trait loci(QTLs) that significantly contribute to high Zn/Se and low Cd accumulation in grains. A chromosome segment substitution line CSSLGCC7 with the PA64s-derived GCC7 allele in the 93-11 background, exhibited steadily higher Mn and lower Cd concentrations in grains than those of 93-11. This elite chromosome segment substitution line(CSSL) was used as the core breeding material to cross with CSSLs harboring other major QTLs for essential mineral elements, especially CSSLGZC6 for grain Zn concentration and CSSLGSC5 for grain Se concentration. The CSSLGCC7+GZC6 and CSSLGCC7+GSC5 exhibited lower Cd concentration with higher Zn and Se concentrations in grains, respectively. Our study thus provides elite materials for rice breeding targeting high Zn/Se and low Cd concentrations in grains. | Chaolei Liu Shilin Ding Anpeng Zhang Kai Hong Hongzhen Jiang Shenglong Yang Banpu Ruan Bin Zhang Guojun Dong Longbiao Guo Dali Zeng Qian Qian Zhenyu Gao | 2020 | Journal of Integrative Plant Biology2020，62，3： | 7 |
| 9 | Analytical validation of GMEX rapid point- of care CYP2C19 genotyping system for the CHANCE-2 trial显示文摘Background and purpose Rapid genotyping is useful for guiding early antiplatelet therapy in patients with high-risk nondisabling ischaemic cerebrovascular events(HR-NICE).Conventional genetic testing methods used in CYP2C19 genotype-guided antiplatelet therapy for patients with HR-NICE did not satisfy the needs of the Clopidogrel in High-Risk Patients with Acute Nondisabling Cerebrovascular Events(CHANCE)-2 trial.Therefore,we developed the rapid-genotyping GMEX(point-of care)system to meet the needs of the CHANCE-2 trial.Methods Healthy individuals and patients with history of cardiovascular diseases(n=408)were enrolled from six centres of the CHANCE-2 trial.We compared the laboratory-based genomic test results with Sanger sequencing test results for accuracy verification.Next,we demonstrated the accuracy,timeliness and clinical operability of the GMEX system compared with laboratory-based technology(YZY Kit)to verify whether the GMEX system satisfies the needs of the CHANCE-2 trial.Results Genotypes reported by the GMEX system showed 100%agreement with those determined by using the YZY Kit and Sanger sequencing for all three CYP2C19 alleles(*2,*3 and*17)tested.The average result’s turnaround times for the GMEX and YZY Kit methods were 85.0(IQR:85.0-86.0)and 1630.0(IQR:354.0-7594.0)min(p<0.001),respectively.Conclusions Our data suggest that the GMEX system is a reliable and feasible point-of care system for rapid CYP2C19 genotyping for the CHANCE-2 trial or related clinical and research applications. | Xia Meng Anxin Wang Guojun Zhang Siying Niu Wei Li Sifei Han Fang Fang Xingquan Zhao Kehui Dong Zening Jin Huaguang Zheng Kelin Chen Hao Li Chengyuan Yang Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 7 |
| 10 | Upregulated KPNA2 promotes hepatocellular carcinoma progression and indicates prognostic significance across human cancer types显示文摘Hepatocellular carcinoma(HCC)is one of the most aggressive cancers worldwide.Identification of the molecular mechanisms underlying the development and progression of HCC is particularly important.Here,we demonstrated the expression pattern,clinical significance,and function of Karyopherin α2(KPNA2)in HCC.The expression of KPNA2 was upregulated in tumor tissue and negatively associated with the survival time,and a significant correlation between KPNA2 expression and aggressive clinical characteristics was established.Both in vitro and in vivo experiments demonstrated that knockdown of KPNA2 reduced migration and proliferation capacities of HCC cells,while over-expression of KPNA2 increased these malignant characteristics.The analysis of the Cancer Genome Atlas cohorts also reveals that high-KPNA2 expression is associated with poor outcome in multiple cancer types.In addition,gene sets enrichment analysis exhibited cell cycle and DNA replication as the top altered pathways in the high-KPNA2 expression group in HCC and other two cancer types.Overall,this study identified KPNA2 as a potential diagnostic and prognostic biomarker in HCC and other neoplasms,probably by regulating cell cycle and DNA replication. | Xinggang Guo Zhiheng Wang Jianing Zhang Qingguo Xu Guojun Hou Yuan Yang Chuanpeng Dong Gang Liu Chenhua Liang Lei Liu Weiping Zhou Hui Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 6 |
| 11 | Genetic analysis of leaffolder resistance in rice显示文摘A double haploid(DH)population,which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid 'CJ06'/'TN1',was used to investigate the genetic basis for rice leaffolder resistance.Using a constructed molecular linkage map,five QTLs for rolled leaves were detected on chromosomes 1,2,3,4,and 8.The positive alleles from CJ06 on chromosomes 3,4,and 8 in-creased the resistance to rice leaffolder,and the alleles from TN1 on chromosomes 1 and 2 also enhanced resistance to leaffolder.The interactions between QTLs were identified and tested,and four conditional interactions were acquired for resistance to rice leaffolder.These loci were located on chromosomes 2,9,10,and 11,respectively.QTL pyramiding indicated that the positive alleles affect resistance to leaffolder.The prospective application of this data in rice breeding was also discussed. | Yuchun Rao Guojun Dong Dali Zeng Jiang Hu Longjun Zeng Zhengyu Gao Guanghen Zhang Longbiao Guo Qian Qian | 2010 | Journal of Genetics and Genomics2010，37，5： | 6 |
| 12 | Quantitative Trait Loci Mapping of Flag-leaf Ligule Length in Rice and Alignment with ZmLG1 Gene显示文摘A doubled haploid(DH) population,which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid 'CJ06'/'TN1',was used in this study.Ligule lengths of flag leaf were investigated for quantitative trait loci(QTL) mapping using the DH population.Five QTLs(qLL-2,qLL-4,qLL-6,qLL-10 and qLL-12) controlling the ligule length(LL) were detected on chromosomes 2,4,6,10 and 12,with the variances explained 11.4%,13.6%,27.8%,22.1% and 11.0%,respectively.Using four known genes of ZmGL1,ZmGL2,ZmGL3 and ZmGL4 in maize from the MaizeGDB,their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping.A ZmLG1 homolog gene,OsLG1 encoding a squamosa promoter binding protein,was located between the markers RM255 and RM280,which is just identical to the interval of qLL-4 on the long arm of chromosome 4.The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution. | Dali Zeng Jiang Hu Guojun Dong Jian Liu Longjun Zeng Guangheng Zhang Longbiao Guo Yihua Zhou Qian Qian | 2009 | Journal of Integrative Plant Biology2009，51，4： | 6 |
| 13 | Identification of a novel tillering dwarf mutant and fine mapping of the TDDL(T) gene in rice (Oryza sativa L.)显示文摘Rice plant architecture is an important agronomic trait that affects the grain yield. To understand the molecular mechanism that controls plant architecture, a tillering dwarf mutant with darker-green leaves derived from an indica cultivar IR64 treated with EMS is characterized. The mutant, designated as tddl(t), is nonallelic to the known tillering dwarf mutants. It is controlled by one recessive nuclear gene, TDDL(T), and grouped into the dn-type dwarfism according to Takeda's definition. The dwarfism of the mutant is independent of gibberellic acid based on the analyses of two GA-mediated processes. The independence of brassinosteroid (BR) and naphthal-3-acetic acid (NAA) of the tddl(t) mutant, together with the decreased size of parenchyma cells in the vascular bundle, indicates that the TDDL(T) gene might participate in another hormone pathway. TDDL(T) is fine mapped within an 85.51 kb region on the long arm of rice chromosome 4, where 20 ORFs are predicted by RiceGAAS (http://gffzz54b87a777dcc48a0hnxcv9bw0c0qc6owp.ffgz.tsg.suse.edu.cn. go.jp/rgadb/). Further cloning of TDDL(T) will benefit both marker assisted selection (MAS) of plant architecture and dissection of the molecular mechanism underlying tillering dwarf in rice. | GAO ZhenYu LIU XiaoHui GUO LongBiao LIU Jian DONG GuoJun HU Jiang HAN Bin QIAN Qian | 2009 | Chinese Science Bulletin2009，54，12： | 4 |
| 14 | The pleiotropic ABNORMAL FLOWER AND DWARF1 affects plant height, floral development and grain yield in rice显示文摘Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present study,we characterized a rice gene, ABNORMAL FLOWER AND DWARF1(AFD1) that determined plant height, floral development and grain yield. The afd1 mutant showed variable defects including the dwarfism, long panicle, low seed setting and reduced grain yield. In addition, abnormal floral organs were also observed in the afd1 mutant including slender and thick hulls, and hull-like lodicules.AFD1 encoded a DUF640 domain protein and was expressed in all tested tissues and organs. Subcellular localization showed AFD1-green fluorescent fusion protein(GFP) was localized in the nucleus. Meantime, our results suggested that AFD1 regulated the expression of cell division and expansion related genes. | Deyong Ren Yuchun Rao Liwen Wu Qiankun Xu Zizhuang Li Haiping Yu Yu Zhang Yujia Leng Jiang Hu Li Zhu Zhenyu Gao Guojun Dong Guangheng Zhang Longbiao Guo Dali Zeng Qian Qian | 2016 | Journal of Integrative Plant Biology2016，58，6： | 4 |
| 15 | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System显示文摘Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2 x35 S::Cas9-sgRNA^(PTGMS2-1) expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo-sensitive genic male-sterile(P/TGMS) lines in T_1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process. | SHEN Lan DONG Guojun ZHANG Yu HU Guocheng ZHANG Qiang HU Guanglian XU Bo REN Deyong HU Jiang ZHU Li GAO Zhenyu ZHANG Guangheng GUO Longbiao ZENG Dali QIAN Qian | 2019 | Rice science2019，26，2： | 3 |
| 16 | Identification and characterization of Mini1, a gene regulating rice shoot development显示文摘The aerial parts of higher plants are generated from the shoot apical meristem(SAM). In this study, we isolated a small rice(Oryza sativa L.) mutant that showed premature termination of shoot development and was named mini rice 1(mini1). The mutant was first isolated from a japonica cultivar Zhonghua11(ZH11) subjected to ethyl methanesulfonate(EMS)treatment. With bulked segregant analysis(BSA) and map-based cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp.The candidate gene Os09g0363900 was predicted to encode a putative adhesion of calyx edges protein ACE(putative HOTHEAD precursor) and genetic complementation experiment confirmed the identity of Mini1. Os09g0363900 contains glucose-methanol-choline(GMC) oxidoreductase and NAD(P)-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD(HTH). Expression analysis indicated Mini1 was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant.We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice. | Yunxia Fang Jiang Hu Jie Xu Haiping Yu Zhenyuan Shi Guosheng Xiong Li Zhu Dali Zeng Guangheng Zhang Zhenyu Gao Guojun Dong Meixian Yan Longbiao Guo Yonghong Wang Qian Qian | 2015 | Journal of Integrative Plant Biology2015，57，2： | 3 |
| 17 | Genome-wide association study and transcriptome analysis reveal new QTL and candidate genes for nitrogen-deficiency tolerance in rice显示文摘The development of rice cultivars with improved nitrogen use efficiency(NUE)is desirable for sustainable agriculture.Achieving this goal depends in part on understanding how rice responds to low soil nitrogen(N)and identifying causative genes underlying this trait.To identify quantitative trait loci(QTL)or genes associated with low N response,we conducted a genome-wide association study(GWAS)using a diverse panel of 230 rice accessions and performed a transcriptomic investigation of rice accessions with differential responses to low N stress at two N levels.We detected 411 GWAS-associated genes in 5 QTL and 2722 differentially expressed genes in response to low N,of which 24 were identified by both methods and ranked according to gene annotations,literature queries,gene expression,and genetic diversity analysis.The large-scale datasets obtained from this study reveal low N-responsive characteristics and provide insights towards understanding the regulatory mechanisms of N-deficiency tolerance in rice,and the candidate genes or QTL would be valuable resources for increasing rice NUE via molecular biotechnology. | Qing Li Xueli Lu Changjian Wang Lan Shen Liping Dai Jinli He Long Yang Peiyuan Li Yifeng Hong Qiang Zhang Guojun Dong Jiang Hu Guangheng Zhang Deyong Ren Zhenyu Gao Longbiao Guo Qian Qian Li Zhu Dali Zeng | 2022 | The Crop Journal2022，10，4： | 3 |
| 18 | Quantificational Etching of AAO Template显示文摘Ni nanowires 被电极淀积在从 命令solution.Well 的 Ni nanowire 数组 withcontrollable 长度当时是的合成电解质的模板用磷的 acidand 的混合物由 AAO 的部分移动做了的多孔的阳极化的 aluminumoxide ( AAO )准备铬的酸( 6 wt % H3PO4:1.8 wt % H3CrO4 ) Ni nanowire 数组的 .The 图象是学习 byscanning 电子显微镜学( SEM )决定在蚀刻时间和 lengthof Ni nanowire arrays.The | Guojun SONG Dong CHEN Zhi PENG Xilin SHE Jianjiang LI Ping HAN | 2007 | Journal of Materials Science & Technology2007，23，3： | 2 |
| 19 | Preparation of porous nanocrystalline TiO_2 electrode by screen-printing technique显示文摘Different paste has been used for preparing porous TiO2 thin film by screen-printing technique, the main component of it comes from commercial TiO2 P25 power. The dye-sensitized solar cell based on this TiO2 thin film without further chemical treatments exhibits high overall conversion efficiency of 5.81%―6.70%, even with low TiO2 content and thin film thickness. The experimental repeatability is nice and the properties of the films are uniform. | XIE DongMei FENG ShuJing LIN Yuan DONG GuoJun XIAO XuRui LI XuePing ZHOU XiaoWen | 2007 | Chinese Science Bulletin2007，52，18： | 2 |
| 20 | Formyl tetrahydrofolate deformylase affects hydrogen peroxide accumulation and leaf senescence by regulating the folate status and redox homeostasis in rice显示文摘It is well established that an abnormal tetrahydrofolate(THF)cycle causes the accumulation of hydrogen peroxide(H_(2)O_(2))and leaf senescence,however,the molecular mechanism underlying this relationship remains largely unknown.Here,we reported a novel rice tetrahydrofolate cycle mutant,which exhibited H_(2)O_(2)accumulation and early leaf senescence phenotypes.Map-based cloning revealed that HPA1 encodes a tetrahydrofolate deformylase,and its deficiency led to the accumulation of tetrahydrofolate,5-formyl tetrahydrofolate and 10-formyl tetrahydrofolate,in contrast,a decrease in 5,10-methenyl-tetrahydrofolate.The expression of tetrahydrofolate cycle-associated genes encoding serine hydroxymethyl transferase,glycine decarboxylase and 5-formyl tetrahydrofolate cycloligase was significantly down-regulated.In addition,the accumulation of H_(2)O_(2)in hpa1 was not caused by elevated glycolate oxidation.Proteomics and enzyme activity analyses further revealed that mitochondria oxidative phosphorylation complex I and complex V were differentially expressed in hpa1,which was consistent with the H_(2)O_(2)accumulation in hpa1.In a further feeding assay with exogenous glutathione(GSH),a non-enzymatic antioxidant that consumes H_(2)O_(2),the H_(2)O_(2)accumulation and leaf senescence phenotypes of hpa1 were obviously compensated.Taken together,our findings suggest that the accumulation of H_(2)O_(2)in hpa1 may be mediated by an altered folate status and redox homeostasis,subsequently triggering leaf senescence. | Erhui Xiong Guojun Dong Fei Chen Chen Zhang Shan Li Yanli Zhang Jahidul Islam Shohag Xiaoe Yang Yihua Zhou Qian Qian Limin Wu Yanchun Yu | 2021 | Science China(Life Sciences)2021，64，5： | 1 |
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