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| 1 | Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model显示文摘AIM: To evaluate the antiviral potency of a new antihepatitis C virus(HCV) antiviral agent targeting the cellular autophagy machinery. METHODS: Non-infected liver slices, obtained from human liver resection and cut in 350 μm-thick slices(2.7 × 106 cells per slice) were infected with cell culture-grown HCV Con1b/C3 supernatant(multiplicity of infection = 0.1) cultivated for up to ten days. HCV infected slices were treated at day 4 post-infection with GNS-396 for 6 d at different concentrations. HCV replication was evaluated by strand-specific real-time quantitative reverse transcription- polymerase chain reaction. The infectivity titers of supernatants were evaluated by foci formation upon inoculation into naive Huh-7.5.1 cells. The cytotoxic effect of the drugs was evaluated by lactate dehydrogenase leakage assays. RESULTS: The antiviral efficacy of a new antiviral drug, GNS-396, an autophagy inhibitor, on HCV infection of adult human liver slices was evidenced in a dosedependent manner. At day 6 post-treatment, GNS-396 EC50 was 158 nmol/L without cytotoxic effect(compared to hydroxychloroquine EC50 = 1.17 μmol/L).CONCLUSION: Our results demonstrated that our ex vivo model is efficient for evaluation the potency of autophagy inhibitors, in particular a new quinoline derivative GNS-396 as antiviral could inhibit HCV infection in a dosedependent manner without cytotoxic effect. | Sylvie Lagaye Sonia Brun Jesintha Gaston Hong Shen Ruzena Stranska Claire Camus Clarisse Dubray Géraldine Rousseau Pierre-Philippe Massault Jerome Courcambeck Firas Bassisi Philippe Halfon Stanislas Pol | 2016 | World Journal of Hepatology2016，8，21： | 5 |
| 2 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 3 |
| 3 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 3 |
| 4 | Graft saving management of hepatic artery thrombosis in pediatric liver transplantation显示文摘 | Massault PP Soubrane O Cardoso J | 1997 | Transplant Proc1997，29，12： | 1 |
| 5 | Efficient replication of primary or culture hepatitis C virus isolates in human liver slices: A relevant ex vivo model of liver infection显示文摘 | Sylvie Lagaye Hong Shen Bertrand Saunier Michelina Nascimbeni Jesintha Gaston Pierre Bourdoncle Laurent Hannoun Pierre‐Philippe Massault Ana?s Vallet‐Pichard Vincent Mallet Stanislas Pol | 2012 | Hepatology2012，，3： | 1 |
| 6 | Bomb 14C time history recorded in two modern stalagmites- Importance for soil organic matter dynamics and bomb 14C distribution over continents显示文摘 | Genty D Vokal B Obelic B Massault M | 1998 | Earth and Planetary Science Letters1998，160，3： | 1 |
| 7 | Renewal rate estimation of groundwater based on radioactive tracers ( 3 H, 14 C) in an unconfined aquifer in a semi-arid area, Iullemeden Basin, Niger显示文摘 | C. Le Gal La Salle C. Marlin C. Leduc J.D. Taupin M. Massault G. Favreau | 2001 | Journal of Hydrology2001，，1： | 1 |
| 8 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 1 |
| 9 | Videolaparoscopic excision of insulinoma,a study of 5 cases显示文摘 | Chapuis Y Bigourdan JM Massault PP | 1998 | Chirurgie1998，123，5： | 1 |
| 10 | Graft saving management of hepatic artery thrombosis in pediatric liver transplantation显示文摘 | Massault PP Soubrane O Cardoso J | 1997 | Transplant Proc1997，29，12： | 1 |
| 11 | Treatment of stage IVA hepatocellular carcinoma: should we reappraise the role of surgel'y? 显示文摘 | Chirica M Scatton O Massault PP | 2008 | Arch Surg2008，143，6： | 1 |
| 12 | Hepatocellular carcinoma developed on compensated cirrhosis: resection as a selection tool for liver transplantation 显示文摘 | Scatton O Zalinski S Terris B Lefevre J H Casali A Massault P P | 2008 | Liver Transpl2008，14，： | 1 |
| 13 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 1 |
| 14 | Carbon transfer dynamics from bomb-14 C andδ13 C time series of a laminated stalagmite from SW France-modeling and comparison with other stalagmite records显示文摘 | Genty D Massault M | 1999 | Geochimica et Cosmochimica Acta1999，63，10： | 1 |
| 15 | Autoimmune hemolytic anemia revealing miliary tuberculosis显示文摘 | Blanche P Rigolet A Massault PP | 2000 | J Infect Dis2000，40，3： | 1 |
| 16 | Adult human liver slice cultures:Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs显示文摘BACKGROUND Liver fibrosis can result in end-stage liver failure and death.AIM To examine human liver fibrogenesis and anti-fibrotic therapies,we evaluated the three dimensional ex vivo liver slice(LS)model.METHODS Fibrotic liver samples(F0 to F4 fibrosis stage according to the METAVIR score)were collected from patients after liver resection.Human liver slices(HLS)were cultivated for up to 21 days.Hepatitis C virus(HCV)infection,alcohol(ethanol stimulation)and steatosis(palmitate stimulation)were examined in fibrotic(F2 to F4)liver slices infected(or not)with HCV.F0-F1 HLS were used as controls.At day 0,either ursodeoxycholic acid(choleretic and hepatoprotective properties)and/or α-tocopherol(antioxidant properties)were added to standard of care on HLS and fibrotic liver slices,infected(or not)with HCV.Expression of the biomarkers of fibrosis and the triglyceride production were checked by quantitative reverse transcription polymerase chain reaction and/or enzymelinked immunosorbent assay.RESULTS The cultures were viable in vitro for 21 days allowing to study fibrosis inducers and to estimate the effect of anti-fibrotic drugs.Expression of the biomarkers of fibrosis and the progression to steatosis(estimated by triglycerides production)was increased with the addition of HCV and/or ethanol or palmitate.From day 15 of the follow-up studies,a significant decrease of both transforming growth factorβ-1 and Procol1A1 expression and triglycerides production was observed when a combined anti-fibrotic treatment was applied on HCV infected F2-F4 LS cultures.CONCLUSION These results show that the human three dimensional ex vivo model effectively reflects the in vivo processes in damaged human liver(viral,alcoholic,nonalcoholic steatohepatitis liver diseases)and provides the proof of concept that the LS examined model permits a rapid evaluation of new anti-fibrotic therapies when used alone or in combination. | Daria Kartasheva-Ebertz Jesintha Gaston Loriane Lair-Mehiri Pierre-Philippe Massault Olivier Scatton Jean-Christophe Vaillant Vladimir Alexei Morozov Stanislas Pol Sylvie Lagaye | 2021 | World Journal of Hepatology2021，13，2： | 0 |
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