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| 1 | Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro显示文摘Sertoli 房间紧密的连接(TJ ) 是血睾丸障碍的关键部件,在它扣押开发在生精的小管以内经历精子发生的细菌房间的地方。神经质地调整的 claudin-11 是在不孕涉及障碍功能和它的鼠科的猛烈结果的批评 transmembrane 蛋白质。我们试图估计份量上到 TJ 功能的 claudin-11 的贡献的意义在 vitro,使用调停 siRNA 的基因 silencing。我们也进行了 occludin 的贡献的分析, TJ 的另一内在的 transmembrane 蛋白质。claudin-11 或 occludin 的 Silencing 在一个不成熟的老鼠 Sertoli 房间文化模型用 siRNA 被进行。Transepithelial 电的抵抗被用来在整个文化估计份量上 TJ 功能。在 siRNA 处理以后的二天,房间为对 mRNA 的 RT-PCR 评价为连接蛋白质或 lyzed 的 immunocytochemical 本地化被修理表示。claudin-11, occludin,或两个的 Silencing 在 55% 的 TJ 功能导致了重要减少(P <0.01 ) , 51%(P <0.01 ) ,并且 62%(P <0.01 ) 分别地。数据在到 Sertoli 房间 TJ 的指向的蛋白质的本地化是有在 mRNA 表示和显著减小的重要减少的伴随物。我们提供 claudin-11 显著地贡献的量的证据(P <0.01 ) 到 Sertoli 房间 TJ 在 vitro 工作。有趣地, occludin,神经质地在不孕被调整然而并非含有直到迟了的成人生活,也是一重要(P <0.01 ) 贡献者到障碍功能。我们的数据与一致在清楚地为这些表明一个角色的 vivo 研究,在维持正常 TJ 障碍的蛋白质组织并且工作。 | Mark J McCabe Caroline FH Foo Marcel E Dinger Peter M Smooker Peter G Stanton | 2016 | Asian Journal of Andrology2016，18，4： | 7 |
| 2 | Colonic stenting versus emergency surgery for acute left-sided malignant colonic obstruction: a multicentre randomised trial显示文摘 | Jeanin E van Hooft Willem A Bemelman Bas Oldenburg Andreas W Marinelli Martijn F Lutke Holzik Marina J Grubben Mirjam A Sprangers Marcel G Dijkgraaf Paul Fockens | 2011 | Lancet Oncology2011，，4： | 3 |
| 3 | Smoothing and Differentiation of Data by Simplified Least Squares Procedures显示文摘 | Savitzky A Marcel J E Golay | 1964 | Analyti- cal Chemistry1964，36，： | 1 |
| 4 | Lipases in the Preparation of β-blockers 显示文摘 | Vincent H M E van Lersel J | 1988 | TIBTECH1988，6，： | 1 |
| 5 | A quantum chemical explanation of the antioxidant activity of flavonoids显示文摘 | SASKIA A B E VAN ACKER MARCEL J DE GROOT el al | 1996 | Chem Res Toxicol1996，9，： | 1 |
| 6 | Mechanical and morphological variation of the human lumbar vertebral cortical and tra becular bone显示文摘 | Marcel E R Rho Jae-Young Ting Y T | 1999 | J Biomed Mater Res1999，44，： | 1 |
| 7 | Evidence for the semireduced primary quinone electron acceptor of photosystem II being a photosensitizer for UVB damage to be the photosynthetic apparatus 显示文摘 | GUSTAVO C R MARCEL A K JANSEN M E VAN DEN NOORT | 2006 | Plant Science2006，170，： | 1 |
| 8 | Elastic properties of microstructural components of human bone tissue as measured by nanoindentation显示文摘 | Rho Jae-Young Marcel E R Ting Y T | 1999 | J Biomed Mater Res1999，45，： | 1 |
| 9 | Oil-cracking processes evidence from synthetic petroleum inclusions显示文摘 | Stephane T Marcel E Jacques P | 2003 | Journal of Geochemical Exploration2003，26，7879： | 1 |
| 10 | Synthesis of the main metabolite(OL-17) of cyclosporin A显示文摘 | Marcel K E Francois N | 1992 | J Org Chem1992，57，9： | 1 |
| 11 | Multi-Slit Spectrometry显示文摘 | GOLAY Marcel J E | 1949 | JOSA1949，39，6： | 1 |
| 12 | Development of three real-time PCR assays to detect peanut allergen residue in processed food products显示文摘 | Elena Scaravelli Marcel Brohée Rosangela Marchelli Arjon J. Hengel | 2008 | European Food Research and Technology2008，，3： | 1 |
| 13 | Native protein recovery from potato fruit juice by ultrafiltration显示文摘 | Harmen J Zwijnenberg Antoine J B Marcel E Boerrigter | 2002 | Desalination2002，144，13： | 1 |
| 14 | Adjustment to climate change is constrained by arrival date in a long distance migrant bird 显示文摘 | Christiaan B Marcel E V | 2001 | Nature2001，411，6835： | 1 |
| 15 | The Global Crisisand Equity Market Contagion显示文摘 | Geert B Michael E Marcel F | 2014 | The Journal of Finance2014，6，： | 1 |
| 16 | Smoothing and differentiation of data by simplified least squares procedures显示文摘 | Abraham S Marcel J E | 1964 | Analytical chemistry1964，36，8： | 1 |
| 17 | Multi-Slit Spectrometry显示文摘 | GOLAY Marcel J E | 1949 | JOSA1949，39，6： | 1 |
| 18 | Development of a monoclonal -based enzyme -linked immunosorbent assay for the coccidiostat salinomycin显示文摘 | Marcel H E Ross C B Loyd D R | 1993 | J Agric Food Chem1993，41，： | 1 |
| 19 | Complementary series 显示文摘 | Golay Marcel J E | 1961 | IRE Trans on Information Theory1961，7，2： | 1 |
| 20 | Expression of distinct RNAs from 3' untranslated regions 显示文摘 | Tim R Mercer Dagmar Wilhelm Marcel E Dinger | 2011 | Nucleic Acids Res2011，39，6： | 1 |
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