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| 1 | Enhancing DNA vaccine potency against hantavirus by co-administration of interleukin-12 expression vector as a genetic adjuvant显示文摘Background The heavy incidence and mortality of hemorrhagic fever with renal syndrome, as well as no specific drugs in curing the disease,clearly indicate the need for development of the more effective hantavirus vaccine. Refining the DNA vaccination strategy to elicit more clinically efficacious immune responses is now under intensive investigation. In the present study, we examined the effects of using an interleukin-12 expression plasmid as a genetic adjuvant to enhance the immune responses induced by a DNA vaccine based on the S gene encoding nucleocapsid protein against hantavirus.Methods BALB/c mice were immunized three times by intramuscular inoculations of DNA vaccine encoding of hantanvirus nucleocapsid protein alone or in combination with a plasmid expressing murine interleukin-12 (pcIL-12). Booster immunizations were employed 2 times at 2-week interval. To evaluate the humoral and cellular immune responses, antigen-specific lymphocyte proliferation and antibody production were assayed by MTT method and ELISA respectively. The level of interleukin-4 and interferon-γ in the splenic lymphocytic cultured supernatant were detected with ELISA kit at day 5, 10, 17, 35 and 42 after primary immunization.Results Antigen-specific IgG antibodies was increased markedly at day 17 in the experiment groups and reached a plateau after day 35. As pcIL-12 co-injected, a significant inhibition of antigen-specific IgG levels was displayed over the period and the antibody mean titre was decreased to only about 1∶50 at day 42 after primary immunization, significantly lower than the group immunized with pcDNA3.1+S alone, in which the mean titre was about 1∶70. Interferon-γ was increased remarkably by the co-injection of pcIL-12 compared with the injection of pcDNA3.1+S alone. However, the production of interleukin-4 was inhibited by pcIL-12 co-injection. Furthermore, pcIL-12 co-injection efficiently enhanced antigen-specific lymphocyte proliferation.Conclusion Humoral and cytokine responses elicited by pcDNA3.1+S inoculation can be modulated by co-inoculation with pcIL-12 and efficiently induced Th1-dominant immune responses. | ZHENGLan-yan MOULing LINSong LURun-ming LUOEn-jie | 2005 | Chinese Medical Journal2005，，4： | 4 |
| 2 | Hormone naive prostate cancer: predicting and maximizing response intervals显示文摘 | Judd W Moul | 2015 | Asian Journal of Andrology2015，17，6： | 4 |
| 3 | Complete genomes of two clinical Staphylococcus aureus strains:evidence for the rapid evolution of virulence and drug resistance显示文摘 | Holden MT Feil EJ Lindsay JA Peacock SJ Day NP Enright MC Foster TJ Moore CE Hurst L Atkin R Barron A Bason N Bentley SD Chillingworth C Chillingworth T Churcher C Clark L Corton C Cronin A Doggett J Dowd L Feltwell T Hance Z Harris B Hauser H Holroyd S Jagels K James KD Lennard N Line A Mayes R Moule S Mungall K Ormond D Quail MA Rabbinowitsch E Rutherford K Sanders M Sharp S Simmonds M Stevens K Whitehead S Barrell BG Spratt BG Parkhill J | 2004 | Proc Natl Acad Sci USA2004，101，26： | 1 |
| 4 | Survival and differentiation of rat spinal cord stem cells grafted into normal and injured adult spinal cord显示文摘 | Chow S Y Moul J Liu Y | 1998 | Soc Neurosci Abstr1998，23，： | 1 |
| 5 | Deciphering the biology of My cobacterium tuberculosis from the complete genome sequence 显示文摘 | Cole S T Brosch R Parkhill J Gamier T Churcher C Harris D Gordon S V Eiglmeier K Gas S Barry C E Tekaia F Badcock K Basham D Brown D Chillingworth T Cormor R Davies R Devlin K FeltweU T Gentles S Hamlin N Holroyd S Hornsby T Jagels K Kroghs A McLean J Moule S Murphy L Oliver K Osborne J Quail M A Rafandream M A Rogers J Rutter S Seeger K Skelton J Squaraes R Squares S Sulston J E Taylo K Whitehead S Barrell B G | 1998 | Nature1998，393，： | 1 |
| 6 | Role for PP2A in ARF signaling to p53显示文摘 | Moule MG Collins CH McCormick F | 2004 | Proc Natl Acad Sci USA2004，101，14： | 1 |
| 7 | Student experiences and mentor views of the use of simulation for learning 显示文摘 | Moule P Wilford A Sales R | 2008 | Nurse Educ Today2008，28，7： | 1 |
| 8 | Synchronous bi lateral testis tumor mixed germ cell and theca cell tumor显示文摘 | Schenkman N S Moul J W Nicely E R | 1993 | Urology1993，42，5： | 1 |
| 9 | Learning on a simu- lator does transfer to clinical practice 显示文摘 | Domuracki KJ Moule CJ Owen H | 2009 | Resuscitation2009，80，3： | 1 |
| 10 | Long-term tolerability and efficacy of degarelix= 5-year results from a phase ]显示文摘 | Crawford ED Shore ND Moul JW | 2014 | Urology2014，83，5： | 1 |
| 11 | Post-radical prostatectomy management options for positive surgical margins: argument for observation显示文摘 | Moul JW | 2009 | Urol Oncol2009，27，1： | 1 |
| 12 | Evaluation of therapeutic approaches with luteinizing hormone-releasing hormone agonists in 2003 显示文摘 | Moul J W Fowler J E Jr | 2003 | Urology2003，62，61： | 1 |
| 13 | Symptom reports in severe chronic insomnia显示文摘 | Moul DE Nofzinger EA Pilkonis PA | 2002 | Sleep2002，25，5： | 1 |
| 14 | Comparativestudy of technetium-99m bone scans and orthopan-tomography in determining mandible invasion inintraoral squamous cell carcinoma显示文摘 | Ahuja RB Soutar DS Moule B | 1990 | Head Neck1990，12，3： | 1 |
| 15 | Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence 显示文摘 | Cole ST Brosch R Parkhill J Garnier T Churcher C Harris D Gordon SV Eiglmeier K Gas S Barry CE Tekaia F Badcock K Basham D Brown D Chillingworth T Connor R Davies R Devlin K Feltwell T Gentles S Hamlin N Holroyd S Hornsby T Jagels K Krogh A Mclean J Moule S Murphy L Oliver K Osborne J Quail MA Rajandream MA Rogers J Rutter S Seeger K Skelton J Squares R Squares S Sulston JE Taylor K Whitehead S Barrell BG | 1998 | Nature1998，393，6685： | 1 |
| 16 | Efficient large-scale development of microsatellites for marker and mapping applications in Brassicacrop species显示文摘 | Lowe A J Moule C Trick M | 2004 | Theoretical and Applied Genetics2004，108，6： | 1 |
| 17 | Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species显示文摘 | Lowe A J Moule C Trick M | 2004 | Theor Appl Genet2004，108，： | 1 |
| 18 | Early versus delayed hormonal therapy for prostate specific antigen only recurrence of prostate cancer after radical prostatectomy显示文摘 | MOUL JW WU H SUN L | 2008 | J Urol2008，179，5： | 1 |
| 19 | Minor traumatic injuries to the permanentdentition 显示文摘 | Moule AJ Moule CA | 2009 | Dent Clin North Am2009，53，4： | 1 |
| 20 | Influence of ethylenediaminetetraacetic acid on the active chlorine content of sodium hypochlorite solutions when mixed in various proportions显示文摘 | Clarkson RM Podlich HM Moule AJ | | 0，，04： | 1 |
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