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| 1 | Exogenous plant MIR168a specifically targets mammalian LDLRAPI: evidence of cross-kingdom regulation by microRNA显示文摘 | Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang | 2012 | Cell Research2012，22，1： | 155 |
| 2 | Recent Advances in Synthesis of Waterborne Polyurethane and Their Application in Water-based Ink:A Review显示文摘Over the last few years,waterborne polyurethane(WPU) was applied to various fibers,adhesives,primers for metals,caulking materials,emulsion polymerization media,paint additives,defoamers,associate thickeners,pigment pastes,textile dyes and biomaterials,resulting in the increasing enthusiasm of researchers to design and synthesize novel WPU with unique properties.Thus,various processes and raw materials have been developed to prepare WPU.This review gives an overview on the developments of WPU mainly derived from novel polyols,analyzes the potential application in water-based inks and presents the probable future research area about water-based inks.Notably,the poly(ε-caprolactone) and poly(lactic acid) are the versatile materials used in WPU synthesis and supply the potential special performance for preparing WPU.Meanwhile,addition-fragmentation chain transfer(RAFT) polymerization and atom transfer radical polymerization(ATRP) processes provide an opportunity to control the chain sequence of WPU and obtain products with the desired performance. | Xing Zhou Yan Li Changqing Fang Shujuan Li Youliang Cheng Wanqing Lei Xiangjie Meng | 2015 | Journal of Materials Science & Technology2015，31，7： | 47 |
| 3 | Agricultural non-point nitrogen pollution control function of dierent vegetation types in riparian wetlands: A case study in the Yellow River wetland in China显示文摘Riparian wetland is the major transition zone of matter, energy and information transfer between aquatic and terrestrial ecosystems and has important functions of water purification and non-point pollution control. Using the field experiment method and an isotope tracing technique, the agricultural non-point nitrogen pollution control function of dierent vegetation types in riparian wetland was studied in the Kouma Section of the Yellow River. The results showed that the retention of agricultural non-point nitrogen pollution by riparian wetland soil occurs mainly in top 0-10 cm layer. The amount of nitrogen retained by surface soils associated with three types of vegetation are 0.045 mg/g for Phragmites communis Trin Linn, 0.036 mg/g for Scirpus triqueter Linn, and 0.032 mg/g for Typha angustifolia Linn, which account for 59.21%, 56.25%, and 56.14% of the total nitrogen interception, respectively. Exogenous nitrogen in 0-10 cm soil layer changes more quickly than in other layers. One month after adding K15NO3 to the tested vegetation, nitrogen content was 77.78% for P. communis Trin, 68.75% for T. angustifolia, and 8.33% for S. triqueter in the surface soil. After three months, nitrogen content was 93.33% for P. communis Trin, 72.22% for S. triqueter, and 37.50% for T. Angustifolia. There are large dierences among vegetation communities respecting to purification of agricultural non-point nitrogen pollution. The nitrogen uptake amount decreases in the sequence: new shoots of P. communis Trin (9.731 mg/g) > old P. communis Trin (4.939 mg/g) > S. triqueter (0.620 mg/g) > T. angustifolia (0.186 mg/g). Observations indicated that the presence of riparian wetlands as buers on and adjacent to stream banks could be recommended to control agricultural non-point pollution. | ZHAO Tongqian XU Huashan HE Yuxiao TAI Chao MENG Hongqi ZENG Fanfu XING Menglin | 2009 | Journal of Environmental Sciences2009，21，7： | 29 |
| 4 | 电针和经皮穴位电刺激技术在生殖医学中的应用:专家共识(英文)显示文摘本文从传统针刺治疗不孕症的选穴依据与现代电针/经皮穴位电刺激技术的相关原理着手,从最优刺激参数选择、穴位辨证、疗程与治疗次数的确定及基本原理等方面阐述了电针/经皮穴位电刺激技术在多囊卵巢综合症、取卵镇痛、卵巢储备功能降低、胚胎移植以及男性少/弱精症等生殖领域中的具体应用。首次对电针/经皮穴位电刺激技术在生殖领域优势病种中的应用进行了系统的分析和总结,在最优刺激参数选择、穴位辨证、疗程与治疗次数的确定等方面提出了一系列专家共识,并为临床实践提供了指导意见和理论依据。 | Fan QU Rong LI Wei SUN Ge LIN Rong ZHANG Jing YANG Li TIAN Guo-gang XING Hui JIANG Fei GONG Xiao-yan LIANG Yan MENG Jia-yin LIU Li-ying ZHOU Shu-yu WANG Yan WU Yi-jing HE Jia-yu YE Song-ping HAN Ji-sheng HAN | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 24 |
| 5 | Association between Vitamin D Insufficiency and the Risk for Gestational Diabetes Mellitus in Pregnant Chinese Women显示文摘Objective To investigate the association between vitamin D deficiency and risk of gestational diabetes mellitus(GDM) in pregnant Chinese women.Methods A nested case-control study was conducted.Clinical and biochemical data were analyzed for 200 subjects with GDM and 200 subjects with normal glucose tolerance(NGT).Results The median(interquartile range) serum 25-hydroxyvitamin D(25OHD) levels were 22.39(17.67,29.38) and 25.86(19.09,34.88) nmol/L in the GDM and NGT groups,respectively.Rates of 25OHD deficiency or insufficiency were significantly higher in the GDM group than in the NGT group.Subjects with 25OHD levels <25 nmol/L had a 1.8-fold higher risk of GDM compared with subjects with higher vitamin D levels.In the GDM group,serum 25OHD was independently associated with HbA1c and insulin resistance after adjusting for confounding factors.In the NGT group,serum 25OHD was independently associated with fasting plasma glucose and systolic blood pressure after adjusting for maternal age and other confounding factors.Conclusion 25OHD insufficiency is very common in Chinese women.Low 25OHD status may be associated with insulin resistance and act as a risk factor for GDM. | WANG Ou NIE Min HU Ying Ying ZHANG Kui LI Wei PING Fan LIU Jun Tao CHEN Li Meng XING Xiao Ping | 2012 | Biomedical and Environmental Sciences2012，25，4： | 21 |
| 6 | Genetic Architecture of Natural Variation in Rice Chlorophyll Content Revealed by a Genome-Wide Association Study显示文摘 | Quanxiu. Wang Weibo Xie Hongkun Xing Ju Yan Xiangzhou Meng Xinglei Li Xiangkui Fu Jiuyue Xu Xingming Lian Sibin Yu Yongzhong Xing Gongwei Wang | 2015 | Molecular Plant2015，8，6： | 20 |
| 7 | Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain显示文摘Objective Our previous study showed that tumor tissue-derived formaldehyde at low concentrations plays an important role in bone cancer pain through activating transient receptor potential vanilloid subfamily member 1 (TRPV1). The present study further explored whether this tumor tissue-derived endogenous formaldehyde regulates TRPV1 expression in a rat model of bone cancer pain, and if so, what the possible signal pathways are during the development of this type of pain. Methods A rat model of bone cancer pain was established by injecting living MRMT-1 tumor cells into the tibia. The formaldehyde levels were determined by high performance liquid chromatography, and the expression of TRPV1 was examined with Western blot and RT-PCR. In primary cultured dorsal root ganglion (DRG) neurons, the expression of TRPV1 was assessed after treatment with 100 μmol/L formaldehyde with or without pre-addition of PD98059 [an inhibitor for extracellular signal-regulated kinase], SB203580 (a p38 inhibitor), SP600125 [an inhibitor for c-Jun N-terminal kinase], BIM [a protein kinase C (PKC) inhibitor] or LY294002 [a phosphatidylinositol 3-kinase (PI3K) inhibitor]. Results In the rat model of bone cancer pain, formaldehyde concentration increased in blood plasma, bone marrow and the spinal cord. TRPV1 protein expression was also increased in the DRG. In primary cultured DRG neurons, 100 μmol/L formaldehyde significantly increased the TRPV1 expression level. Pre-incubation with PD98059, SB203580, SP600125 or LY294002, but not BIM, inhibited the formaldehyde-induced increase of TRPV1 expression. Conclusion Formaldehyde at a very low concentration up-regulates TRPV1 expression through mitogen-activated protein kinase and PI3K, but not PKC, signaling pathways. These results further support our previous finding that TRPV1 in peripheral afferents plays a role in bone cancer pain. | Ying Han Yan Li Xlng Xlao Jia Liu Xiang-Ling Meng Feng-Yu Liu Guo-Gang Xing You Wan | 2012 | Neuroscience Bulletin2012，28，2： | 19 |
| 8 | Analysis of CD8+ Treg cells in patients with ovarian cancer: a possible mechanism for immune impairment显示文摘 | Shuping Zhang | 2015 | Cellular & Molecular Immunology2015，12，5： | 17 |
| 9 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 10 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 11 | Levels and dynamic changes of serum fibroblast growth factor 23 in hypophosphatemic rickets/osteomalacia显示文摘背景 Hypophosphatemic 佝偻病 / 软骨病是骨头的有缺点的矿化作用由于 hypophosphatemia 和低 1,25-dihydroxy 维生素 D 描述的一组疾病。在磷酸盐动态平衡的规定探索成纤维细胞生长因素 23 的角色(FGF-23 ) ,我们与 hypophosphatemic 在健康个人并且在病人测量了这个生长因素的传播集中佝偻病 / 软骨病。有 hypophosphatemic 佝偻病 / 软骨病的十九个病人在 hypophosphatemic 被包括的方法组织(HP, 12 女性和 7 男、吝啬的年龄是 30 年) ,并且 19 个健康匹配年龄的个人担任了控制组。完整的长度 FGF-23 碎片被二地点的连接酶的 immunosorbent assay.Results 平均数 FGF-23 集中测量在 HP 组是显著地更高的((87.4 芤吗?? | XIA Wei-bo JIANG Yan LI Mei XING Xiao-ping WANG Ou HU Ying-ying ZHANG Hua-bing LIU Huai-cheng MENG Xun-wu ZHOU Xue-ying | 2010 | Chinese Medical Journal2010，，9： | 14 |
| 12 | Experimental study on the bolt–cable combined supporting technology for the extraction roadways in weakly cemented strata显示文摘Aiming at the characteristics of the poor steady ability, the short stable time and severe deformation behavior of weakly cemented soft surrounding rock around extraction roadway, a bolt–cable combined supporting technology was proposed. Numerical simulation was performed by using FLAC3 D software to study the effects of different supporting systems. The simulation result proves that those supporting systems have good practical values. Based on real-time monitoring and analysis of the deformation of surrounding rock and the stress of supporting structure, real time information of deformation of surrounding rock and stress state of supporting structure of extraction roadway within weakly cemented strata was obtained. Monitoring results show that large deformation and failure of surrounding rock of extraction roadway within weakly cemented strata can be effectively controlled by the bolt–cable combined supporting technology, which ensures the long-term stability and safety of surrounding rock and supporting structure. | Meng Qingbin Han Lijun Sun Jingwu Min Fengqing Feng Wei Zhou Xing | 2015 | International Journal of Mining Science and Technology2015，25，1： | 13 |
| 13 | Early Permian Tarim Large Igneous Province in northwest China显示文摘Tarim Large Igneous Province(TLIP)is the second Late Paleozoic LIPs in China after the recognition of Emeishan LIP,and is a hot research topic in geosciences.On the basis of the analysis of research history about TLIP,this paper summarizes the research result during last twenty years and suggests the key research area in the future.The residual distribution range of TLIP is up to 250000 km2,and the largest residual thickness is 780 m.The eruption of basalt happened during 290–288 Ma and belongs to LIPs magmatic event with fast eruption of magma.The lithological units of the TLIP include basalt,diabase,layered intrusive rock,breccia pipe mica-olivine pyroxenite,olivine pyroxenite,gabbro,ultramafic dyke,quartz syenite,quartz syenite porphyry and bimodal dyke.The basalt and diabase of TLIP exhibit OIB-like trace element patterns and enrichment of LILE and HFSE,and mainly belong to high TiO2series.There is an obvious difference in isotope among the basalt from Keping and the basalt and dibase from the northern Tarim Basin.The basalt from Keping with negative Nd and high REE value derives from enriched mantle,and the diabase and basalt from the northern Tarim Basin with positive Nd and low REE value are related to depleted mantle.The crust uplifting in the Early Permian and the development of picrite and large scale dyke and formation of large scale V-Ti-Magnetite deposit in Wajilitag area support the view that the TLIP is related to mantle plume.The TLIP has a temporal-spatial relationship with Permian basic to ultra-basic igneous rock,which is distributed widely in Central Asia,and they represent a tectono-magmatic event with very important geodynamic setting.This paper also suggests that the deep geological process,the relation with mantle plume,mineralization,the relation with environmental change and biological evolution,and the geodynamics of the TLIP will be the key research topics in the future. | YANG ShuFeng CHEN HanLin LI ZiLong LI YinQi YU Xing LI DongXu MENG LiFeng | 2013 | Science China Earth Sciences2013，56，12： | 12 |
| 14 | The efficacy and safety of calcitriol and/or Caltrate D in elderly Chinese women with low bone mass显示文摘 | Wei-bo XIA Zhong-lan ZHANG Hong-fu WANG Xun-wu MENG Ying ZHANG Guo-ying ZHU Xiao-ping XING Jian-li LIU Li-hua WANG Yan JIANG Shi-fang WENG Tao XU Ying-ying HU Wei YU Jun-ping TIAN | 2009 | Acta Pharmacologica Sinica2009，30，3： | 11 |
| 15 | A 12-year cohort study on the efficacy of plasmaderived hepatitis B vaccine in rural newborns显示文摘AIM To understand the anti-HBs persistenceand the long-term preventive efficacy in ruralnewborns after vaccination with plasma-derivedhepatitis B vaccine.METHODS In the time of expanded program onimmunization(EPI),the newborns werevaccinated with 10μg×3 doses of hepatitis Bvaccine and 762 newborns who were HBsAgnegative after primary immunization wereselected for cohort observation from 1986 to1998.Their serum samples were detectedqualitatively and quantitatively for hepatitis Binfecting markers,including HBsAg,anti-HBsand anti-HBc by SPRIA Kits.The annual HBsAgpositive conversion rate was counted by life-table method.RESULTS①The anti-HBs positive rate was94.44% for the babies born to HBsAg negativemothers and 84.21% for those born to HBsAgpositive mothers in the 1st year afterimmunization,and dropped to 51.31% and52.50% in the 12th year respectively.GMT valuewas dropped from 31.62 to 3.13 and 23.99 to 3.65in the 2nd to the 12th year respectively.Therewas a marked drop in GMT at the 3rd to the 5thyear,and in anti-HBs positive rate at the 9th tothe 10th year.②In the period of 12 yearsobservation,the person-year HBsAg positive conversion rates were 0.12%(5/4150.0)innewborns born to HBsAg negative mothers and0.20%(1/508.0)in those born to HBsAgpositive mothers,and none of the HBsAgpositive converted children became HBsAgchronic carriers.Compared with the baselinebefore immunization,the protective rates were97.19% and 95.32% respectively.CONCLUSION The protective efficacy ofplasma-derived hepatitis B vaccine persisted atleast 12 years,and a booster dose seems notnecessary within at least 12 years after theprimary three-doses immunization to newbornsborn to HBsAg negative mothers. | Hong Bin Liu Zong Da Meng Jing Chen Ma Chang Quan Han Ying Lin Zhang Zhan Chun Xing Yu Wei Zhang Yu Zhong Liu Hui Lin Cao | 2000 | World Journal of Gastroenterology2000，6，3： | 11 |
| 16 | Decolorization of Methyl Orange by a new clay-supported nanoscale zero-valent iron:Synergetic effect,efficiency optimization and mechanism显示文摘In this study, a novel nanoscale zero-valent iron(n ZVI) composite material was successfully synthesized using a low-cost natural clay, 'Hangjin 2#clay'(HJ clay) as the support and tested for the decolorization of the azo dye Methyl Orange(MO) in aqueous solution by n ZVI particles. According to the characterization and MO decolorization experiments, the sample with 5:1 HJ clay-supported n ZVI(HJ/n ZVI) mass ratio(HJ-n ZVI5) showed the best dispersion and reactivity and the highest MO decolorization efficiency. With the same equivalent Fe0 dosage, the HJ-n ZVI1 and HJ-n ZVI5 samples demonstrated a synergetic effect for the decolorization of MO: their decolorization efficiencies were much higher than that achieved by physical mixing of HJ clay and n ZVIs, or the sum of HJ clay and n ZVIs alone. The synergetic effect was primarily due to the improved dispersion and more effective utilization of the n ZVI particles on/in the composite materials. Higher decolorization efficiency of MO was obtained at larger HJ-n ZVI dosage, higher temperature and under N2 atmosphere, while the MO initial concentration and p H were negatively correlated to the efficiency. HJ clay not only works as a carrier for n ZVI nanoparticles, but also contributes to the decolorization through an 'adsorption-enhanced reduction' mechanism. The high efficiency of HJ-n ZVI for decontamination gives it great potential for use in a variety of remediation applications. | Xiaoguang Li Ying Zhao Beidou Xi Xiaoguang Meng Bin Gong Rui Li Xing Peng Hongliang Liu | 2017 | Journal of Environmental Sciences2017，29，2： | 9 |
| 17 | Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress显示文摘Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs. | Yaning Cui Xi Zhang Meng Yu Yingfang Zhu Jingjing Xing Jinxing Lin | 2019 | Science China(Life Sciences)2019，62，5： | 9 |
| 18 | Progress on Porous Ceramic Membrane Reactors for Heterogeneous Catalysis over Ultrafine and Nano-sized Catalysts显示文摘Heterogeneous catalysts with ultrafine or nano particle size have currently attracted considerable attentions in the chemical and petrochemical production processes, but their large-scale applications remain challenging because of difficulties associated with their efficient separation from the reaction slurry. A porous ceramic membrane reactor has emerged as a promising method to solve the problem concerning catalysts separation in situ from the reaction mixture and make the production process continuous in heterogeneous catalysis. This article presents a review of the present progress on porous ceramic membrane reactors for heterogeneous catalysis, which covers classification of configurations of porous ceramic membrane reactor, major considerations and some important industrial applications. A special emphasis is paid to major considerations in term of application-oriented ceramic membrane design, optimization of ceramic membrane reactor performance and membrane fouling mechanism. Finally, brief concluding remarks on porous ceramic membrane reactors are given and possible future research interests are also outlined. | JIANG Hong MENG Lie CHEN Rizhi JIN Wanqin XING Weihong XU Nanping | 2013 | Chinese Journal of Chemical Engineering2013，21，2： | 9 |
| 19 | The Leu477 and Leu613 of ORF2-Encoded Protein Are Critical in Forming Neutralization Antigenic Epitope of Hepatitis E Virus Genotype 4显示文摘Hepatitis E virus (HEV) genotype 4 was originally identified in China. Its neutralization antigenic epitopes have not been characterized. Recently, we identified a neutralizing monoclonal antibody (mAb) 1G10, which was generated following immunization of mice with p166Chn, a recombinant protein comprising 464-629 amino acids (aa) of the HEV genotype 4 capsid protein. In this study, a panel of 22 N- and/or C-terminal truncated and 6 site-directed mutated p166Chn proteins were prepared. Only those N- or C-terminal truncated proteins containing the region 477-613 aa could react with the mAb 1G10, suggesting the neutralization epitope of HEV genotype 4 is located between aa477 and aa613. However, a both N- and C-terminal truncated protein, pN477-C613, neither reacted to 1G10 nor elicited neutralizing antibodies in mice, while another both terminal truncated protein, pN472-C617, did, suggesting the flanking regions of the pN477-C613 could help to stabilize and allow presentation of the neutralization epitope to the immune system. Substituting Leu477 and/or Leu613 with the polar, uncharged threonine (Thr) caused ≥ 50% reduction of the mutants' immunoreactivity to 1G10, whereas replacement by hydrophobic phenylalanine (Phe) made little impact on the immunoreactivity, revealing functional associations between hydrophobicity of aa at positions 477 and 613 and the antigenicity of p166Chn. These data suggested Leu477 and Leu613 are critical in forming the neutralization epitope of HEV genotype 4. | Hongmei Zhang Xing Dai Xiangnian Shan Jsihong Meng | 2008 | Cellular & Molecular Immunology2008，5，6： | 9 |
| 20 | Injectable ECM hydrogel for delivery of BMSCs enabled full-thickness meniscus repair in an orthotopic rat model显示文摘Meniscal injuries have poor intrinsic healing capability and are associated with the development of osteoarthritis.Decellularized meniscus extracellular matrix(mECM)has been suggested to be efficacious for the repair of meniscus defect.However,main efforts to date have been focused on the concentration,crosslinking density and anatomical region dependence of the mECM hydrogels on regulation of proliferation and differentiation of adult mesenchymal stem cells(MSCs)in vitro 2D or 3D culture.A systematic investigation and understanding of the effect of mECM on encapsulated MSCs response and integrative meniscus repair by in vivo rat subcutaneous implantation and orthotopic meniscus injury model will be highly valuable to explore its potential for clinical translation.In this study,we investigated the in situ delivery of rat BMSCs in an injectable mECM hydrogel to a meniscal defect in a SD rat model.Decellularized mECM retained essential proteoglycans and collagens,and significantly upregulated expression of fibrochondrogenic markers by BMSCs versus collagen hydrogel alone in vitro 3D cell culture.When applied to an orthotopic model of meniscal injury in SD rat,mECM is superior than collagen I scaffold in reduction of osteophyte formation and prevention of joint space narrowing and osteoarthritis development as evidenced by histology and micro-CT analysis.Taken together,these results indicate mECM hydrogel is a highly promising carrier to deliver MSCs for long-term repair of meniscus tissue,while preventing the development of osteoarthritis. | Gang Zhong Jun Yao Xing Huang Yixuan Luo Meng Wang Jinyu Han Fei Chen Yin Yu | 2020 | Bioactive Materials2020，5，4： | 9 |
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