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| 1 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 2 | Procedural complexity independent of P2Y12 reaction unit(PRU)values is associated with acute in situ thrombosis in Pipeline flow diversion of cerebral aneurysms显示文摘background Acute in situ thrombosis is an ischaemic phenomenon during Pipeline embolisation device(PED)procedures with potentially high morbidity and mortality.There is controversy regarding the role of platelet function testing with P2Y12 assay as a predictor of intraprocedural thromboembolic events.There is limited knowledge on whether procedural complexity influences these events.Methods Data were collected retrospectively on 742 consecutive PED cases at a single institution.Patients with intraprocedural acute thrombosis were compared with patients without these events.results A cohort of 37 PED cases with acute in situ thrombosis(mean age 53.8 years,mean aneurysm size 8.4 mm)was matched with a cohort of 705 PED cases without intraprocedural thromboembolic events(mean age 56.4 years,mean aneurysm size 6.9 mm).All patients with in situ thrombosis received intra-arterial and/or intravenous abciximab.The two groups were evenly matched in patient demographics,previous treatment/subarachnoid hemorrhage(SAH)and aneurysm location.There was no statistical difference in postprocedural P2Y12 reaction unit(PRU)values between the two groups,with a mean of 156 in the in situ thrombosis group vs 148 in the control group(p=0.5894).Presence of cervical carotid tortuosity,high cavernous internal carotid artery grade,need for multiple PED and vasospasm were not significantly different between the two groups.The in situ thrombosis group had statistically significant longer fluoroscopy time(60.4 vs 38.4 min,p<0.0001),higher radiation exposure(3476 vs 2160 mGy,p<0.0001),higher rates of adjunctive coiling(24.3% vs 8.37%,p=0.0010)and higher utilisation of balloon angioplasty(37.8% vs 12.2%,p<0.0001).Clinically,the in situ thrombosis cohort had higher incidence of major and minor stroke,intracerebral haemorrhage and length of stay.Conclusions Predictors of procedural complexity(higher radiation exposure,longer fluoroscopy time,adjunctive coiling and need for balloon angioplasty)are associated with acute thrombotic events during PED placement,independent of PRU values. | Bowen Jiang Matthew T Bender Erick M Westbroek Jessica K Campos Li-Mei Lin Risheng Xu Rafael J Tamargo Judy Huang Geoffrey P Colby Alexander L Coon | 2018 | Stroke & Vascular Neurology2018，3，3： | 2 |
| 3 | Adaptation of HIV-1to human leukocyte antigen class I显示文摘 | Kawashima Y Pfafferott K Frater J Matthews P Payne R Addo M Gatanaga H Fujiwara M Hachiya A Koizumi H Kuse N Oka S Duda A Prendergast A Crawford H Leslie A Brumme Z Brumme C Allen T Brander C Kaslow R Tang J Hunter E Allen S Mulenga J Branch S Roach T John M Mallal S Ogwu A Shapiro R Prado J G Fidler S Weber J Pybus O G Klenerman P Ndung'u T Phillips R Heckerman D Harrigan P R Walker B D Takiguchi M Goulder P | 2009 | Nature2009，458，7238： | 1 |
| 4 | Comparative Life- cycleAir Emissions of Coal,Domestic Natural Gas,LNG,and SNG forElectricity Generation 显示文摘 | Jaramillo P Griffin W M Matthews H S | 2007 | Environmental Science & Technology2007，41，17： | 1 |
| 5 | Functional subdivisions within anterior cingulated cortex and their relationship to autonomic nervous system function显示文摘 | Matthews S C Paulus M P Simmons A N Nelesen R A Dimsdale J E | 2004 | NeuroImage2004，22，： | 1 |
| 6 | Structure-function studies of the human immunodeficiency virus type 1 matrix protein, pl7显示文摘 | Cannon P M Matthews S Clark N | 1997 | J Virol1997，71，5： | 1 |
| 7 | A randomized trial of tigecycline versus ampicillin-sulbactam or amoxicillin-cla-vulanate for the treatment of complicated skin and skin struc- ture infections显示文摘 | Matthews P Alpert M Rahav G | 2012 | BMC Infect Dis2012，12，： | 1 |
| 8 | A randomized trial of tigecycline versus ampicillin-sulbactam or amoxicillin- clavulanate for the treatment of complicated skin and skin structure infe,ctions显示文摘 | Matthews P Alpert M Rahav G | 2012 | BMC Infect Dis2012，12，： | 1 |
| 9 | Dendritic cells mediate the induction of polyfunctional human IL17-producing cells(Th17-1 cells)enriched in the bone marrow of patients with myeloma显示文摘 | Dhodapkar K M Barbuto S Matthews P | 2008 | Blood2008，112，7： | 1 |
| 10 | Morphology and the Internal Structure of Words 显示文摘 | DEVLIN J T JAMISON H L MATTHEWS P M | 2004 | Pro- ceedings of the National Academy of Sciences2004，101，14： | 1 |
| 11 | Marker gene elimination from transgenic barley,using co-transformation with adjacent' twin T-DNAs' on a standard Agrobacterium transformation vector显示文摘 | Matthews P R Wang B M | 2001 | Molecular Breeding2001，，7： | 1 |
| 12 | Hamiltonian results in K1,3-free graphs显示文摘 | Matthews M M Sumner D P | 1984 | J Graph Theory1984，8，： | 1 |
| 13 | Chemotherapyrelated myelosuppression as a marker of survival in epithelial ovarian cancer patients显示文摘 | Rocconi R P Matthews K S Kemper M K | 2008 | Gynecol Oncol2008，108，2： | 1 |
| 14 | Middle ear electromagnetic semi - implantable hearing device : resuhs of the phase II SOUNDTEC direct system clinical trial 显示文摘 | Hough J V Matthews P Wood M W | 2002 | Otol Neurotol2002，23，6： | 1 |
| 15 | Laser capture microdissection (LCM) and comparative microarray expression analysis of syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by soybean cyst nematode (Heterodera glycines)显示文摘 | Klink V P Overall C C Alkharouf N MacDonald M H Matthews B F | | 0，，06： | 1 |
| 16 | Experimental studies in the LENS supersonic and hypersonic tunnels for hypervelocity vehicle performance and code validation 显示文摘 | Michael S H Timothy P W Matthew M | 2008 | AIAA2008，2505，： | 1 |
| 17 | Time Dependent Inversion of Geodetic Data显示文摘 | Segall P Matthews M | 1997 | JGR1997，102，22： | 1 |
| 18 | Analysis of serial measurements in medical research显示文摘 | Matthews JN Altrnan DG Campbell M J Royston P | | 0，，： | 1 |
| 19 | Acute endophthalmitis following intravitreal triamcinolone acetonide injection显示文摘 | Darius M Moshfeghi Peter K Kaiser Ingrid U Scott Jonathan E Sears Matthew Benz Juan P Sinesterra Richard S Kaiser Sophie J Bakri Raj K Maturi Jonathan Belmont Paul M Beer Timothy G Murray Hugo Quiroz-Mercado William F Mieler | 2003 | American Journal of Ophthalmology2003，，5： | 1 |
| 20 | A randomized trial of high-versus conventional-dose cytarabine in consolidation chemotherapy for adult de novo acute myeloid leukemia in first remission after induction therapy containing high-dose cytarabine显示文摘 | KENNETH F BRADSTOCK J P MATTHEWS R M | 2005 | Blood2005，105，： | 1 |
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