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| 1 | ELASTIC MODULUS REDUCTION METHOD FOR LIMIT LOAD EVALUATION OF FRAME STRUCTURES显示文摘A new strategy for elastic modulus adjustment is proposed based on the element bearing ratio (EBR),and the elastic modulus reduction method (EMRM) is proposed for limit load evaluation of frame structures. The EBR is defined employing the generalized yield criterion,and the reference EBR is determined by introducing the extrema and the degree of uniformity of EBR in the structure. The elastic modulus in the element with an EBR greater than the reference one is reduced based on the linear elastic finite element analysis and the equilibrium of strain energy. The lower-bound of limit-loads of frame structures are analyzed and the numerical example demonstrates the flexibility,accuracy and effciency of the proposed method. | Lufeng Yang Bo Yu Yongping Qiao | 2009 | Acta Mechanica Solida Sinica2009，22，2： | 20 |
| 2 | The next-generation sequencing technology and application显示文摘As one of the key technologies in biomedical research,DNA sequencing has not only improved its productivity with an exponential growth rate but also been applied to new areas of application over the past few years.This is largely due to the advent of newer generations of sequencing platforms,offering ever-faster and cheaper ways to analyze sequences.In our previous review,we looked into technical characteristics of the nextgeneration sequencers and provided prospective insights into their future development.In this article,we present a brief overview of the advantages and shortcomings of key commercially available platforms with a focus on their suitability for a broad range of applications. | Xiaoguang Zhou Lufeng Ren Qingshu Meng Yuntao Li Yude Yu Jun Yu | 2010 | Protein & Cell2010，1，6： | 13 |
| 3 | Coupling Rice with Fish for Sustainable Yields and Soil Fertility in China显示文摘About 30 million hectares of rice are cultivated annually in China(FAOSTAT,2019).Because most rice fields are wellirrigated and suitable for fish living(‘fish’here refers to various aquatic animals like carps,prawns,crabs,soft shell turtles,etc.)(Fernando,1993),many efforts have been made to determine how well rice farming and aquaculture can be coupled as a rice-fish system(RFS),of which the same land and water produces both rice and aquatic products.With the technological guidance of RFSs and political supports from the Ministry of Agriculture and Rural Affairs of China since 2010(Supplemental Table 1),multiple types of large-scale,high-efficiency and technologically intensive RFSs are now being demonstrated in the main rice-growing areas of China(National Fisheries Technology Extension Centre,2019),and in total about 1.2 million hectares of rice fields are under RFSs. | GUO Liang HU Liangliang ZHAO Lufeng SHI Xiaoyu JI Zijun DING Lilian REN Weizheng ZHANG Jian TANG Jianjun CHEN Xin | 2020 | Rice science2020，27，3： | 11 |
| 4 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 5 | Prediction of eukaryotic gene structures based on multilevel optimization显示文摘Computational gene structure prediction, which is valuable for finding new genes and understanding the composition of genomes, plays a very important role in various kinds of genome projects. For eukaryotic gene structures, however, the prediction accuracy of existing methods is still limited. This paper presents a method of pre-dicting eukaryotic gene structures based on multilevel opti-mization. The complicated problem of predicting gene structure in eukaryotic DNA sequence containing multiple genes can be decomposed into a series of sub-problems at several levels with decreasing complexity, including the gene level (single-exon gene, multi-exon gene), the element level (exon, intron, etc.), and the feature level (functional site sig-nals, codon usage preference, etc.). On the basis of this de-composition, a multilevel model for the prediction of complex gene structures is created by a multilevel optimization proc-ess, in which the models dealing with sub-problems at low complexity level are first optimized respectively, and then optimally combined together to form models for those sub-problems at higher complexity level. Based on the multi-level model, a dynamic programming algorithm is designed to search for optimal gene structures from DNA sequences, and a new program GeneKey (1.0) for the prediction of eu-karyotic gene structures is developed. Testing results with widely used datasets demonstrate that the prediction accura-cies of GeneKey (1.0) at the nucleotide level, exon level and gene level are all higher than that of the well known program GENSCAN. A web server of GeneKey(1.0) is available at http://gffzze1e99242f9d74955hko5pqn95vo9c6q95.ffgz.tsg.suse.edu.cn. | ZHOUYanhong YANGLei WANGHui LUFeng WANHonghui | 2004 | Chinese Science Bulletin2004，49，4： | 7 |
| 6 | Metal nanoparticles induced photocatalysis显示文摘Photocatalysis induced by light absorption of metal nanoparticles(NPs) has emerged as a promising strategy for exploiting efficient visible-light-responsive composites for solar-energy conversion. In this review, we first introduce the light absorption of metal NPs and the mechanisms proposed in metal-induced photocatalysis(MIP). Then, its applications in water splitting, artificial photosynthesis and inert molecular activation are summarized. To address the challenge of low efficiency in this field, strategies in promoting catalytic activity are reviewed, and particular attention is paid to the particle-size effect of metal. Finally, the challenges and possible development directions of MIP are briefly discussed. | Lequan Liu Xinnan Zhang Lufeng Yang Liteng Ren Defa Wang Jinhua Ye | 2017 | National Science Review2017，4，5： | 7 |
| 7 | Light Trapping Effect in Wing Scales of Butterfly Papilio peranthus and Its Simulations显示文摘 | Zhiwu Han Shichao Niu Lufeng Zhang Zhenning Liu Luquan Ren | 2013 | Journal of Bionic Engineering2013，10，2： | 7 |
| 8 | Fast fine acquisition algorithm of GPS receiver aided by INS information显示文摘Acquisition time of global position system (GPS) receiver, which is the main factor contributes to time to first fix (TTFF), can be shortened by estimating the Doppler frequency shift through external inertial navigation system (INS) information and almanac data and reducing the searching area. The traditional fast acquisition is analyzed, the fast acquisition of the GPS receiver aided is presented by INS information, and the signal is fine captured by spectrum zooming. Then the algorithm is simulated by sampled GPS intermediate frequency (IF) signal and the result verifies that this acquisition can dramatically improve the capability of GPS receiver and reduce its acquisition time. | Lufeng Zhu Chunxi Zhang Zhiqiang Ma | 2011 | Journal of Systems Engineering and Electronics2011，22，2： | 6 |
| 9 | Degassing of deep-sourced CO_(2) from Xianshuihe-Anninghe fault zones in the eastern Tibetan Plateau显示文摘A large number of gases are releasing from the medium-high temperature geothermal fields distributed along the large-scale strike-slip fault zones in the southeastern margin of the Tibetan Plateau.In this study,11 hot spring water and the associated bubbling gas samples were collected along the Xianshuihe-Anninghe fault zones(XSH-ANHFZ)and analyzed for chemical and isotopic compositions.The δ^(18)_(H_(2)O) and δD_(H_(2)O) values indicate that hot spring waters are predominantly meteoric origin recharged from different altitudes.Most water samples are significantly enriched in Na^(+) and HCO_(3) due to the dissolution of regional evaporites,carbonates and Na-silicates.^(3)He/^(4)He ratios of the gas samples are 0.025-2.73 times the atmospheric value.The ^(3)He/^(4)He ratios are high in the Kangding region where the dense faults are distributed,and gradually decrease with increasing distance from Kangding towards both sides along the Xianshuihe fault zones(XSHFZ).Hydrothermal fluids have dissolved inorganic carbon(DIC)concentrations from 2 to 42 mmol L^(-1),δ^(13)C_(DIC) from -6.9‰ to 1.3‰,δ^(13)C_(CO_(2)) from -7.2‰ to -3.6‰ and Δ^(14)C from -997‰ to -909‰.Combining regional geochemical and geological information,the CO_(2)sources can be attributed to deep-sourced CO_(2)from mantle and metamorphism of marine carbonate,and shallow-sourced CO_(2)from the dissolution of marine carbonate and biogenic CO_(2).The mass balance model shows that 11±6% of the DIC is sourced from the dissolution of shallow carbonate minerals,9±8% formed by pyrolysis of sedimentary organic matter,80±9% derived from deep metamorphic origin and mantle-derived CO_(2).Among them,the deep-sourced CO_(2)in Anninghe fault zones(ANHFZ)is merely metamorphic carbon,whereas ca.12% and ca.88% of the deep-sourced CO_(2)in the XSHFZ are derived from the mantle and metamorphic carbon,respectively.The average deep-sourced CO_(2)flux in the Kangding geothermal field is estimated to be 160 ta^(-1).If all the hot springs in various fault zones in the southeastern margin of the Tibetan Plateau are taken into account,the regional deep-sourced CO_(2)flux would reach ca.10^(5) ta^(-1).These results show that the deep-sourced CO_(2)released from nonvolcanic areas might account for a considerable proportion of the total amount of global deep-sourced carbon degassing,which should be paid more attention to. | Sheng XU Lufeng GUAN Maoliang ZHANG Jun ZHONG Wei LIU Xian'gang XIE Congqiang LIU Naoto TAKAHATA Yuji SANO | 2022 | Science China Earth Sciences2022，65，1： | 6 |
| 10 | Antiferromagnetically ordered Mott insulator and d+id superconductivity in twisted bilayer graphene: a quantum Monte Carlo study显示文摘Using exact quantum Monte Carlo method, we examine the recent novel electronic states seen in magicangle graphene superlattices. From the Hubbard model on a double-layer honeycomb lattice with a rotation angle θ=1:08°, we reveal that an antiferromagnetically ordered Mott insulator emerges beyond a critical U_c at half filling, and with a small doping, the pairing with d+id symmetry dominates over other pairings at low temperature. The effective d+id pairing interaction strongly increases as the on-site Coulomb interaction increases, indicating that the superconductivity is driven by electron-electron correlation. Our non-biased numerical results demonstrate that the twisted bilayer graphene shares the similar superconducting mechanism of high temperature superconductors, which is a new and ideal platform for further investigating the strongly correlated phenomena. | Tongyun Huang Lufeng Zhang Tianxing Ma | 2019 | Science Bulletin2019，64，5： | 5 |
| 11 | A complete genome assembly of Glaciecola mesophila sp. nov. sequenced by using BIGIS-4 sequencer system显示文摘Using a pyrosequencing-based custom-made sequencer BIGIS-4, we sequenced a Gram-negative bacterium Glaciecola mesophila sp. nov. (Gmn) isolated from marine invertebrate specimens. We generated 152043 sequencing reads with a mean high-quality length of 406 bp, and assembled them using the BIGIS-4 post-processing module. No systematic low-quality data was detected beyond expected homopolymer-derived errors. The assembled Gmn genome is 5144318 bp in length and harbors 4303 annotated genes. A large number of metabolic genes correspond to various nutrients from surface marine invertebrates. Its abundant cold-tolerant and cellular signaling and related genes reveal a fundamental adaptation to low-temperature marine environment. | YUAN LiNa REN LuFeng LI YunTao HAN WeiJing YU Yong CHU YaNan LIU GuiMing YU Dan TENG MingJing WANG Liang WANG XuMin ZHOU XiaoGuang YU YuDe YU Jun | 2011 | Science China(Life Sciences)2011，54，9： | 4 |
| 12 | Comparative analysis of four essential Gracilariaceae species in China based on whole transcriptomic sequencing显示文摘Three Gracilaria species,G.chouae,G.blodgettii,G.vermiculophylla and a close relative species,Gracilariopsis lemaneiformis which is now nominated as Gracilaria lemaneiformis,are the typically indigenous species which are important resources for the production of special proteins,phycobilisomes,special carbohydrates,and agar in China.In this study,de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time.Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G.lemaneiformis and other three Gracilaria species.Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species.The genes encoding allophycocyanin,phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G.lemaneiformis had close relationship to genus Gracilaria,as well as that within genus Gracilaria,G.chouae had closer relationship to G.vermiculophylla rather than to G.blodgettii.The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae. | XU Jiayue SUN Jing YIN Jinlong WANG Liang WANG Xumin LIU Tao CHI Shan LIU Cui REN Lufeng WU Shuangxiu YU Jun | 2014 | Acta Oceanologica Sinica2014，33，2： | 3 |
| 13 | ILK and PRDX1 are prognostic markers in squamous cell/adenosquamous carcinomas and adenocarcinoma of gallbladder显示文摘 | Jinghe Li Zhu-lin Yang Xuebao Ren Qiong Zou Yuan Yuan Lufeng Liang Meigui Chen Senlin Chen | 2013 | Tumor Biology2013，，1： | 3 |
| 14 | Optimal interface surface determination for multi-axis freeform surface machining with both roughing and finishing显示文摘In the current practice of multi-axis machining of freeform surfaces, the interface surface between the roughing and finishing process is simply an offset surface of the nominal surface. While there have already been attempts at minimizing the machining time by considering the kinematic capacities of the machine tool and/or the physical constraints such as the cutting force, they all target independently at either the finishing or the roughing process alone and are based on the simple premise of an offset interface surface. Conceivably, since the total machining time should count that of both roughing and finishing process and both of them crucially depend on the interface surface, it is natural to ask if, under the same kinematic capacities and the same physical constraints, there is a nontrivial interface surface whose corresponding total machining time will be the minimum among all the possible(infinite) choices of interface surfaces, and this is the motivation behind the work of this paper. Specifically, with respect to the specific type of iso-planar milling for both roughing and finishing, we present a practical algorithm for determining such an optimal interface surface for an arbitrary freeform surface. While the algorithm is proposed for iso-planar milling, it can be easily adapted to other types of milling strategy such as contour milling. Both computer simulation and physical cutting experiments of the proposed method have convincingly demonstrated its advantages over the traditional simple offset method. | Lufeng CHEN Pengcheng HU Ming LUO Kai TANG | 2018 | Chinese Journal of Aeronautics2018，31，2： | 3 |
| 15 | The Characteristics Analysis and Cogging Torque Optimization of a Surface-Interior Permanent Magnet Synchronous Motor显示文摘This paper proposes optimal stator skewed slot analytical method for cogging torque reduction in surface-interior permanent magnet synchronous motor(SIPMSM)and analyzes the characteristics of SIPMSM.The series-parallel equivalent magnetic circuit models(EMCMs)of SIPMSM is built based on the characteristics of magnetic circuits,which is used to design the basic electromagnetic parameters of SIPMSM.Analytical expressions of cogging torque are derived from applying analytical techniques.Stator skewed slot for cogging torque minimum is adopted,and the stator skewed slot pitch is confirmed based on the analytical expressions of the resultant cogging torque.The cogging torque,torque ripple,back electromotive force(back-EMF),power-angle characteristics,efficiency and power factor of SIPMSM are analyzed by establishing 3-dimensional finite element model(3-D-FED)of SIPMSM with stator skewed slot and straight slot.It is shown that the comprehensive performance of optimized SIPMSM is improved as confirmed by finite element analysis and analytical calculation results. | Jikai Si Suzhen Zhao Lufeng Zhang Ruiwu Cao Wenping Cao | 2018 | Chinese Journal of Electrical Engineering2018，4，4： | 3 |
| 16 | Transcriptome-wide evolutionary analysis on essential brown algae(Phaeophyceae)in China显示文摘Brown algae(Chromista,Ochrophyta,Phaeophyceae) are a large group of multicellular algae that play important roles in the ocean's ecosystem and biodiversity.However,poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field.In this study,we sequenced the de novo transcriptome of 18 major species of brown algae in China,covering six orders and seven families,using the high-throughput sequencing platform Illumina HiSeq 2000.From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum,we identified 108 nuclear-generated orthologous genes and clarified the phylogenetic relationships among these brown algae based on a multigene method.These brown algae could be separated into two clades: Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmarestiale-Fucales.The former was at the base of the phylogenetic tree,indicating its early divergence,while the latter was divided into two branches,with Order Fucales diverging from Orders Ectocarpales,Laminariales,and Desmarestiale.In our analysis of taxonomy-contentious species,Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina,respectively,while Petalonia fascia showed possible relation to genus Scytosiphon.The study provided molecular evidence for the phylogenetic taxonomy of brown algae. | SUN Jing WANG Liang WU Shuangxiu WANG Xumin XIAO Jingfa CHI Shan LIU Cui REN Lufeng ZHAO Yuhui LIU Tao YU Jun | 2014 | Acta Oceanologica Sinica2014，33，2： | 2 |
| 17 | MicroRNA-9 as a paradoxical but critical regulator of cancer metastasis:Implications in personalized medicine显示文摘Metastasis,is a development of secondary tumor growths at a distance from the primary site,and closely related to poor prognosis and mortality.However,there is still no effective treatment for metastatic cancer.Therefore,there is an urgent need to find an effective therapy for cancer metastasis.Plenty of evidence indicates that miR-9 can function as a promoter or suppressor in cancer metastasis and coordinate multistep of metastatic process.In this review,we summarize the different roles of miR-9 with the corresponding molecular mechanisms in metastasis of twelve common cancers and the multiple mechanisms underlying miR-9-mediated regulation of metastasis,benefiting the further research of miR-9 and metastasis,and hoping to bridge it with clinical applications. | Yichen Liu Qiong Zhao Tao Xi Lufeng Zheng Xiaoman Li | 2021 | Genes & Diseases2021，8，6： | 2 |
| 18 | Human Pharyngeal Microbiome May Play A Protective Role in Respiratory Tract Infections显示文摘The human pharyngeal microbiome, which resides at the juncture of digestive and respiratory tracts, may have an active role in the prevention of respiratory tract infections, similar to the actions of the intestinal microbiome against enteric infections. Recent studies have demonstrated that the pharyngeal microbiome comprises an abundance of bacterial species that interacts with the local epithelial and immune cells, and together, they form a unique micro-ecological system.Most of the microbial species in microbiomes are obligate symbionts constantly adapting to their unique surroundings. Indigenous commensal species are capable of both maintaining dominance and evoking host immune responses to eliminate invading species. Temporary damage to the pharyngeal microbiome due to the impaired local epithelia is also considered an important predisposing risk factor for infections. Therefore, reinforcement of microbiome homeostasis to prevent invasion of infection-prone species would provide a novel treatment strategy in addition to antibiotic treatment and vaccination. Hence continued research efforts on evaluating probiotic treatment and developing appropriate procedures are necessary to both prevent and treat respiratory infections. | Zhancheng Gao Yu Kang Jun Yu Lufeng Ren | 2014 | Genomics, Proteomics & Bioinformatics2014，12，3： | 2 |
| 19 | Synthesis and proper- ties of carboxymethyl kudzu root starch 显示文摘 | Wang Lufeng Pan Siyi Hu Hao | 2010 | Carbohydrate Polymers2010，80，1： | 1 |
| 20 | Determination of cinepazide maleate in Rat Plasma by LLE Using LC-MS-MS J显示文摘 | Guanyang Lin Jiayin Zhu Lufeng Hu | | 0，，7： | 1 |
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