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| 1 | 张氏江蓠microRNAs鉴定及其基因家族系统进化显示文摘张氏江蓠是江蓠属的重要代表,具有宝贵的工业生产价值。随着海洋环境的恶化,江蓠资源亟待进一步开发与利用。作为基因转录后表达的重要调控子,microRNA (miRNA)在植物组织器官发育、生理调节、胁迫应答等方面起着关键的调控作用。然而,迄今仍未有张氏江蓠miRNA(gch-miRNA)信息的报道,极大地阻碍了江蓠属非编码RNA的相关研究。运用生物信息学技术,通过gch-ESTs与miRBase数据库的比对分析,共鉴定到6条gch-miRNAs。序列特征分析结果显示,鉴定的gch-miRNA序列基本可信,并在其它植物间有保守性和多态性特征。颈环引物qRT-RCR的试验验证进一步证实了鉴定结果的可靠性。部分miRNA基因家族的NJ系统进化结果显示:gch-miRNA位于进化树的外类群;miRNA的进化与物种本身的进化并不同步。TargetFinder筛选获得8条gch-miRNA靶标序列,psRNATarget预测其抑制类型均为切割抑制。首次公布了张氏江蓠miRNA及其靶标信息,综合分析了部分miRNA基因家族在不同植物物种间的系统进化关系。研究结果为张氏江蓠非编码RNA研究,高品质藻种分子选育及其资源的开发利用奠定了基础。 | 宋韡 尹旭岗 何中石 王伟 樊悦 高帆 | 2020 | 生物学杂志2020，37，3： | 2 |
| 2 | Transcriptome sequencing of essential marine brown and red algal species in China and its significance in algal biology and phylogeny显示文摘Most phaeophytes(brown algae) and rhodophytes(red algae) dwell exclusively in marine habitats and play important roles in marine ecology and biodiversity.Many of these brown and red algae are also important resources for industries such as food,medicine and materials due to their unique metabolisms and metabolites.However,many fundamental questions surrounding their origins,early diversification,taxonomy,and special metabolisms remain unsolved because of poor molecular bases in brown and red algal study.As part of the 1 000 Plant Project,the marine macroalgal transcriptomes of 19 Phaeophyceae species and 21 Rhodophyta species from China's coast were sequenced,covering a total of 2 phyla,3 classes,11 orders,and 19 families.An average of 2 Gb per sample and a total 87.3 Gb of RNA-seq raw data were generated.Approximately 15 000 to 25 000 unigenes for each brown algal sample and 5 000 to 10 000 unigenes for each red algal sample were annotated and analyzed.The annotation results showed obvious differences in gene expression and genome characteristics between red algae and brown algae;these differences could even be seen between multicellular and unicellular red algae.The results elucidate some fundamental questions about the phylogenetic taxonomy within phaeophytes and rhodophytes,and also reveal many novel metabolic pathways.These pathways include algal CO2 fixation and particular carbohydrate metabolisms,and related gene/gene family characteristics and evolution in brown and red algae.These findings build on known algal genetic information and significantly improve our understanding of algal biology,biodiversity,evolution,and potential utilization of these marine algae. | WU Shuangxiu SUN Jing CHI Shan WANG Liang WANG Xumin LIU Cui LI Xingang YIN Jinlong LIU Tao YU Jun | 2014 | Acta Oceanologica Sinica2014，33，2： | 0 |
| 3 | 海洋经济红藻江蓠科的分类研究现状及存在的问题显示文摘江蓠科红藻是重要的经济海藻,用途十分广泛。然而,近年来受分子生物学技术引入等的影响,其分类学地位引起了极大争议。针对这个问题,本研究以争议比较大的江蓠属(Gracilaria)、拟江蓠(龙须菜)属(Gracilariopsis)和多穴藻属(Polycavernosa)为对象,总结归纳了其物种多样性、国内外研究进展、存在的问题、学者试图解答的问题以及部分自己的研究结果。以期为该类群的研究提供相对详细、客观的参考数据。 | 丁兰平 杨楠 刘金梅 姜晶晶 黄冰心 | 2020 | 应用海洋学学报2020，39，1： | 0 |
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