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| 1 | Mortality and Morbidity of Extremely Low Birth Weight Infants in the Mainland of China: A Multi-center Study显示文摘 | Hui-Jia Lin Li-Zhong Du Xiao-Lu Ma Li-Ping Shi Jia-Hua Pan Xiao-Mei Tong Qiu-Ping Li Jian-Guo Zhou Bing Yi Ling Liu Yun-Bing Chen Qiu-Fen Wei Hui-Qing Wu Mei Li Cui-Qing Li Xi-Rong Gao Shi-Wen Xia Wen-Bin Li Chao-Ying Ya Ling He Kun Liang Xiao-Yu Zhou Shu-Ping Han Qin Lyu Yin-Ping Qiu Wen Li Dong-Mei Chen Hong-Ru Lu Xiao-Hong Liu Hong Liu Zhen-Lang Lin Li Liu Jia-Jun Zhu Hong Xiong Shao-Jie Yue Si-Qi Zhuang | 2015 | Chinese Medical Journal2015，，20： | 49 |
| 2 | Analysis of DNA methylation in different maize tissues显示文摘DNA methylation plays an important role in gene expression regulation during biological development and tissue differentiation in plants. This study adopted methylation-sensitive Amplified fragment length polymorphism (AFLP) to compare the levels of DNA cyto- sine methylation at CCGG sites in tassel, bracteal leaf, and ear leaf from maize inbred lines, 18 White and 18 Red, respectively, and also examined specific methylation patterns of the three tissues. Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel. Meanwhile, different methylation levels were ob- served in the same tissue from the inbred lines, 18 White and 18 Red. Full methylation of internal cytosine was the dominant type in the maize genome. The differential methylation patterns in the three tissues were observed. In addition, sequencing of nine differentially me- thylated fragments and the subsequent blast search revealed that the cytosine methylated 5′-CCGG-3′sequences were distributed in re- peating sequences, in the coding and noncoding regions. Southern hybridization was used to verify the methylation polymorphism. These results clearly demonstrated the power of the MSAP technique for large-scale DNA methylation detection in the maize genome, and the complexity of DNA methylation change during plant growth and development. The different methylation levels may be related to specific gene expression in various tissues. | Yanli Lu, Tingzhao Rong, Moju Cao Maize Research Institute of Sichuan Agriculture University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Ya’an, 625014, China | 2008 | Journal of Genetics and Genomics2008，35，1： | 27 |
| 3 | Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons显示文摘β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in vitro.However,because of the short survival time of the differentiated cells,clinical applications for this technique are limited.As such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron microscopy.The results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time extended.Taken together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell death.However,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications. | Yanan Cai Xiaodong Yuan Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 19 |
| 4 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 5 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 6 | Effects of therapies for regulating and reinforcing lung and kidney on osteoporosis in rats with chronic obstructive pulmonary disease显示文摘OBJECTIVE:To evaluate the efficacy and long-term effects of the three therapies for regulating and reinforcing lung and kidney(reinforcing lung and invigorating spleen,reinforcing lung and replenishing kidney,and supplementing Qi and nourishing kidney) in Traditional Chinese Medicine(TCM) on osteoporosis in rats with chronic obstructive pulmonary disease.METHODS:Totally 120 rats were randomly divided into control,model,Bufeijianpi,Bufeiyishen,Yiqizishen,aminophyline groups.Repeated smoke inhalations and bacterial infections were used to duplicate the stable Chronic obstructive pulmonary disease rat model.Normal saline was given to the air control and model groups,while Bufeijianpi granule,Bufeiyishen granule,and Yiqizishen granule,and aminophylline were administrated to rats in the Bufeijianpi,Bufeiyishen,Yiqizishen,and aminophylline groups respectively from weeks 9 through 20.Another 12 weeks without medicines to observe the long-term effect.Rats were sacrificed at week 20 and week 32.Bone mass density(BMD),bone mineral content(BMC),morphology of the femoral head,lung function,and levels of serum interleukin(IL)-1β,IL-6,and tumor necrosis factor-a were detected.RESULTS:At weeks 20 and 32,tidal volume,peak expiratory flow and expiratory flow at 50% tidal volume in the three TCM-treated groups were higher than those in the model group(P < 0.05).Femur weight,BMD,and BMC were significantly higher in the three TCM-treated groups and the aminophylline-treated group compared with the model group(P < 0.01),except for BMC in the Yiqizishen-treated group at week 20.CONCLUSION:Bufeijianpi,Bufeiyishen,and Yiqizishen granules show good effects in the prevention and treatment of osteoporosis,which can alleviate airflow limitations and inflammation,improve BMD and BMC of the femur,and have favorable long-term effects. | Tian Yange Li Ya Li Jiansheng Li Suyun Jiang Suli Wang Ying Lu Xiaofan Li Weiwei | 2015 | Journal of Traditional Chinese Medicine2015，35，2： | 13 |
| 7 | Prospects for intelligent rehabilitation techniques to treat motor dysfunction显示文摘More than half of stroke patients live with different levels of motor dysfunction after receiving routine rehabilitation treatments.Therefore,new rehabilitation technologies are urgently needed as auxiliary treatments for motor rehabilitation.Based on routine rehabilitation treatments,a new intelligent rehabilitation platform has been developed for accurate evaluation of function and rehabilitation training.The emerging intelligent rehabilitation techniques can promote the development of motor function rehabilitation in terms of informatization,standardization,and intelligence.Traditional assessment methods are mostly subjective,depending on the experience and expertise of clinicians,and lack standardization and precision.It is therefore difficult to track functional changes during the rehabilitation process.Emerging intelligent rehabilitation techniques provide objective and accurate functional assessment for stroke patients that can promote improvement of clinical guidance for treatment.Artificial intelligence and neural networks play a critical role in intelligent rehabilitation.Multiple novel techniques,such as braincomputer interfaces,virtual reality,neural circuit-magnetic stimulation,and robot-assisted therapy,have been widely used in the clinic.This review summarizes the emerging intelligent rehabilitation techniques for the evaluation and treatment of motor dysfunction caused by nervous system diseases. | Cong-Cong Huo Ya Zheng Wei-Wei Lu Teng-Yu Zhang Dai-Fa Wang Dong-Sheng Xu Zeng-Yong Li | 2021 | Neural Regeneration Research2021，16，2： | 12 |
| 8 | Ultrastructure and electrophysiology of astrocytes differentiated from adult adipose-derived stromal cells显示文摘进在 vitro 的神经房间的背景脂肪质导出 stromal 房间( ADSC )区别广泛地正在成为 studied.However ,跟随区别的星形细胞上,并且特别地在成熟和 electrophysiology.In 上有很少报告这研究,我们使用了各种各样的方法决定有 isobutylmethylxanthine ( IBMX )的 导出ADSC 的星形细胞 maturity.Methods 化学药品正式就职被用来区分成年 ADSC 进hematoxylin曙红跟随的星形细胞(他)染色观察形态学和 | OU Ya YUAN Xiao-dong CAI Ya-nan LU Yan-hui | 2011 | Chinese Medical Journal2011，，17： | 12 |
| 9 | Gene-Gene Interaction of GJB2, SOD2, and CAT on Occupational Noise-induced Hearing Loss in Chinese Han Population显示文摘The effects of genetic factors on the noise-induced hearing loss(NIHL)are still unclear.In the present study,eight single-nucleotide polymorphisms(SNPs)included rs1227049 and rs3802711(CDH23),rs1695(GSTP1),rs137852540(GJB2),rs2289274(PMCA2),rs4880(SOD2),rs7943316,and rs769214 within CAT that might associated with NIHL were further validated | WANG Sheng Li YU Lu Gang LIU Ren Ping ZHU Wan Zhan GAO Wei Min XUE Li Ping JIANG Xu ZHANG Ya Han YI Ding CHEN Dong ZHANG Yong Hong | 2014 | Biomedical and Environmental Sciences2014，27，12： | 12 |
| 10 | A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes显示文摘The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time. | Ya Ou Xiaodong Yuan Yanan Cai Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 12 |
| 11 | Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment显示文摘A couple with a proband child of GJB2(encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis(PGD), noninvasive prenatal testing(NIPT), and noninvasive prenatal diagnosis(NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2 c.235del C heterozygous embryo resulted in a singleton pregnancy. At the 13 th week of gestation, genomic DNA(g DNA) from the trio family and cell-free DNA(cf DNA) from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by invasive procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing impairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders. | XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu | 2015 | Science China(Life Sciences)2015，58，9： | 12 |
| 12 | Molecular Characterization of a Leaf Senescence-Related Transcription Factor BrWRKY75 of Chinese Flowering Cabbage显示文摘WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence. | TAN Xiaoli FAN Zhongqi LI Lulu WU Ya KUANG Jianfei LU Wangjin CHEN Jianye | 2016 | Horticultural Plant Journal2016，2，5： | 11 |
| 13 | Preparation of Pt/C Catalyst with Solid Phase Reaction Method显示文摘The Pt/C catalyst was prepared with solid phase reaction method (Pt/C(S)) for the first time. Its performances were compared with that prepared by the traditional liquid phase reaction method. The results demonstrate that the electrocatalytic activity of Pt/C catalyst with solid phase reaction method for methanol oxidation is higher than that with liquid phase reaction method. XRD and TEM measurements indicate that the Pt/C(S) possesses low crystalline extent and small particle size. | Ya Wen TANG Hui YANG Wei XING Tian Hong LU | 2002 | Chinese Chemical Letters2002，13，5： | 10 |
| 14 | MicroTom Metabolic Network: Rewiring Tomato Metabolic Regulatory Network throughout the Growth Cycle显示文摘Tomato(Solanum lycopersicum)is a major horticultural crop worldwide and has emerged as a preeminent model for metabolic research.Although many research efforts have focused on the analysis of metabolite differences between varieties and species,the dynamics of metabolic changes during the tomato growth cycle and the regulatory networks that underlie these changes are poorly understood.In this study,we integrated high-resolution spatio-temporal metabolome and transcriptome data to systematically explore the metabolic landscape across 20 major tomato tissues and growth stages.In the resulting MicroTom Metabolic Network,the 540 detected metabolites and their co-expressed genes could be divided into 10 distinct clusters based on their biological functions.Using this dataset,we constructed a global map of the major metabolic changes that occur throughout the tomato growth cycle and dissected the underlying regulatory network.In addition to verifying previously well-established regulatory networks for important metabolites,we identified novel transcription factors that regulate the biosynthesis of important secondary metabolites such as steroidal glycoalkaloids and flavonoids.Our findings provide insights into spatiotemporal changes in tomato metabolism and generate a valuable resource for the study of metabolic regulatory processes in model plants. | Yan Li Yang Chen Lu Zhou Shengjie You Heng Deng Ya Chen Saleh Alseekh Yong Yuan Rao Fu Zixin Zhang Dan Su Alisdair R.Fernie Mondher Bouzayen Tao Ma Mingchun Liu Yang Zhang | 2020 | Molecular Plant2020，13，8： | 10 |
| 15 | Fourth C hinese National Consensus Report on the management of H elicobacter pylori infection显示文摘 | Wen Zhong Liu Yong Xie Hong Cheng Nong Hua Lu Fu Lian Hu Wan Dai Zhang Li Ya Zhou Ye Chen Zhi Rong Zeng Chong Wen Wang Shu Dong Xiao Guo Zong Pan Pin Jin Hu | 2013 | Journal of Digestive Diseases2013，，5： | 9 |
| 16 | AduoLa Fuzhenglin Down-regulates Microwave-induced Expression of β_1-adrenergic Receptor and Muscarinic Type 2 Acetylcholine Receptor in Myocardial Cells of Rats显示文摘This paper is aimed to study the effect of ADL on expression ofβ1-AR and M2-AchR in myocardial cells of rats exposed to microwave radiation.Immunohistochemistry,Western blot and image analysis were used to detect the expression ofβ1-AR and M2-AchR in myocardial cells at 7 and 14 | ZHANG Jing PENG Rui Yun GAO Ya Bing WANG Shui Ming YANG Lei Lei ZHAO Li DONG Ji YAO Bin Wei CHANG Gong Min XIONG Lu | 2014 | Biomedical and Environmental Sciences2014，27，3： | 9 |
| 17 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 18 | Boosting electrocatalytic water splitting via metal-metalloid combined modulation in quaternary Ni-Fe-P-B amorphous compound显示文摘Design and synthesis of highly efficient and cost-effective bifunctional catalysts for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)remain a big challenge.Herein,a quaternary amorphous nanocompound Ni-Fe-P-B has been synthesized by a facile,scalable co-reduction method.The Ni-Fe-P-B exhibits high electrocatalytic activity and outstanding durability for both HER and OER,delivering a current density of 10 mA·cm^-2 at overpotentials of 220 and 269 mV,respectively.When loaded on carbon fiber paper(CFP)as a bifunctional catalyst,the Ni-Fe-P-B@CFP electrode requires a low cell voltage of 1.58 V to obtain 10 mA·cm^-2 for overall water splitting with negligible recession over 60 h.The excellent catalytic performances of Ni-Fe-P-B mainly benefit from the metal-metalloid combined composition modulation and the unique amorphous structure.This work provides new insights into the design of robust bifunctional catalysts for water splitting,and may promote the development of multicomponent amorphous catalysts. | Wukui Tang Xiaofang Liu Ya Li Yanhui Pu Yao Lu Zhiming Song Qiang Wang Ronghai Yu Jianglan Shui | 2020 | Nano Research2020，13，2： | 8 |
| 19 | Energy dependence on modes of electric activities of neuron driven by different external mixed signals under electromagnetic induction显示文摘Energy supply and release play an important role in individual neuron and neural network. In this paper, the electrical activities and Hamilton energy of neuron are investigated when external mixed signals(i.e., the periodic stimulus current and the periodic electromagnetic field) are imposed on the neuron under the electromagnetic induction. As a result, the Hamilton energy is much dependent on the mode transition, the multiple electric activity modes and the numerical analysis of Hamilton energy are more complicated under various parameters. When the periodic high-low frequency electromagnetic radiation is imposed in neuron, it is found that the electrical activities are more complex, and the changing of energy is obvious. In addition, the response of electrical activity and Hamilton energy is much dependent on the changing of amplitude A, B when the external high-low frequency signal is imposed on the neuron, meanwhile, the energy of bursting state is lower than the one of spiking state. It can be used for investigation about the energy coding in the neuron even the neuron networks. | LU LuLu JIA Ya XU Ying GE MengYan YANG LiJian ZHAN Xuan | 2019 | Science China(Technological Sciences)2019，62，3： | 8 |
| 20 | Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions显示文摘β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K+ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions. | Xiaodong Yuan Yanan Cai Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，34： | 8 |
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