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| 1 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 2 | Expression Profiles of SIRT1 and APP Genes in Human Neuroblastoma SK-N-SH Cells Treated with Two Epigenetic Agents显示文摘In our previous studies, significant hypermethylation of the sirtuin 1(SIRT1) gene and demethylation of the b-amyloid precursor protein(APP) gene were found in patients with Alzheimer's disease(AD) compared with the normal population. Moreover, the expression of SIRT1 was significantly decreased while that of APP was increased in AD patients. These results indicated a correlation of DNA methylation with gene expression levels in AD patients. To further investigate the epigenetic mechanism of gene modulation in AD, we used two epigenetic drugs, the DNA methylation inhibitor 5-aza-20-deoxycytidine(DAC) and the histone deacetylase inhibitor trichostatin A(TSA), to treat human neuroblastoma SK-N-SH cells in the presence of amyloid b-peptide Ab25–35(Ab25–35). We found that DAC and TSA had different effects on the expression trends of SIRT1 and APP in the cell model of amyloid toxicity. Although other genes, such as microtubule-associated protein s, presenilin 1, presenilin 2, and apolipoprotein E, were up-regulated after Ab25–35treatment, no significant differences were found after DAC and/or TSA treatment. These results support the evidence in AD patients and reveal a strong correlation of SIRT1/APP expression with DNA methylation and/or histone modification, which may help understand the pathogenesis of AD. | Yaping Hou Fanghua Wang Linping Cheng Tao Luo Jie Xu Huaqiao Wang | 2016 | Neuroscience Bulletin2016，32，5： | 2 |
| 3 | Spatiotemporal variations of ambient air pollutants and meteorological influences over typical urban agglomerations in China during the COVID-19 lockdown显示文摘To investigate the air quality change during the COVID-19 pandemic,we analyzed spatiotemporal variations of six criteria pollutants in nine typical urban agglomerations in China using ground-based data and examined meteorological influences through correlation analysis and backward trajectory analysis under different responses.Concentrations of PM2.5,PM10,NO2,SO2 and CO in urban agglomerations respectively decreased by 18%–45%(30%–62%),17%–53%(22%–39%),47%-64%(14%–41%),9%–34%(0%–53%)and 16%-52%(23%–56%)during Lockdown(Post-lockdown)period relative to Pre-lockdown period.PM2.5 pollution events occurred during Lockdown in Beijing-Tianjin-Hebe(BTH)and Middle and South Liaoning(MSL),and daily O3 concentration rose to gradeⅡstandard in Post-lockdown period.Distinct from the nationwide slump of NO2 during Lockdown period,a rebound(~40%)in Post-lockdown period was observed in Cheng-Yu(CY),Yangtze River Middle-Reach(YRMR),Yangtze River Delta(YRD)and Pearl River Delta(PRD).With slightly higher wind speed compared with 2019,the reduction of PM2.5(51%–62%)in Post-lockdown period is more than2019(15%–46%)in HC(Harbin-Changchun),MSL,BTH,CP(Central Plain)and SP(ShandongPeninsula),suggesting lockdown measures are effective to PM2.5 alleviation.Although O3 concentrations generally increased during the lockdown,its increment rate declined compared with 2019 under similar sunlight duration and temperature.Additionally,unlike HC,MSL and BTH,which suffered from additional(>30%)air masses from surrounding areas after the lockdown,the polluted air masses reaching YRD and PRD mostly originated from the long-distance transport,highlighting the importance of joint regional governance. | Linping Fan Shuang Fu Xin Wang Qingyan Fu Haohao Jia Hao Xu Guimei Qin Xue Hu Jinping Cheng | 2021 | Journal of Environmental Sciences2021，33，8： | 0 |
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