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| 1 | Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis显示文摘类胡萝卜素在植物和 phytoene desaturase 基因(PDS3 ) 在许多生理的过程起一个重要作用把重要的酶之一编码为类胡萝卜素生合成小径。这里,我们报导 T-DNA 插入异种 ofPDS3 基因的鉴定和分析。功能的互补证实 thepds3 异种的白化体和矮子显型源于 PDS3 基因的功能的混乱。叶绿体开发在 pds3 异种在专业版质体阶段被逮捕。进一步的分析证明高级 ofphytoene 在 pds3 异种被积累。外长的 GA (3 ) 的增加能部分救矮子显型,建议 pds3 异种的矮子显型可能由于 GAdeficiency。Microarray 和 RT-PCR 分析证明那破坏 PDS3 基因导致了涉及至少 20 条新陈代谢的小径的基因表示变化,包括在类胡萝卜素,叶绿素,和 GA 的许多基因的抑制生合成小径。我们的数据建议在 pds3 异种的积累的 phytoene 可能在某些否定反馈起一个重要作用影响多样的细胞的小径的基因表示。 | Genji Qin Hongya Gu Ligeng Ma Yiben Peng Xing Wang Deng Zhangliang Chen Li-Jia Qu | 2007 | Cell Research2007，17，5： | 39 |
| 2 | CRISPR/Cas9-mediated disruption of TaNP1 genes results in complete male sterility in bread wheat显示文摘Male sterile genes and mutants are valuable resources in hybrid seed production for monoclinous crops.High genetic redundancy due to allohexaploidy makes it difficult to obtain the nuclear recessive male sterile mutants through spontaneous mutation or chemical or physical mutagenesis methods in wheat.The emerging effective genome editing tool,CRISPR/Cas9 system,makes it possible to achieve simultaneous mutagenesis in multiple homoeoalleles.To improve the genome modification efficiency of the CRISPR/Cas9 system in wheat,we compared four different RNA polymerase(Pol)Ⅲpromoters(TaU3 p,TaU6 p,OsU3 p,and OsU6 p)and three types of sgRNA scaffold in the protoplast system.We show that the TaU3 promoter-driven optimized sgRNA scaffold was most effective.The optimized CRISPR/Cas9 system was used to edit three TaNP1 homoeoalleles,whose orthologs,OsNP1 in rice and ZmIPE1 in maize,encode a putative glucose-methanol-choline oxidoreductase and are required for male sterility.Triple homozygous mutations in TaNP1 genes result in complete male sterility.We further demonstrated that anyone wild-type copy of the three TaNP1 genes is sufficient for maintenance of male fertility.Taken together,this study provides an optimized CRISPR/Cas9 vector for wheat genome editing and a complete male sterile mutant for development of a commercially viable hybrid wheat seed production system. | Jian Li Zheng Wang Guangming He Ligeng Ma Xing Wang Deng | 2020 | Journal of Genetics and Genomics2020，47，5： | 12 |
| 3 | Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells显示文摘 | Shuai Fang Chao Wang Jian Xiang Liang Cheng Xuejiao Song Ligeng Xu Rui Peng Zhuang Liu | 2014 | Nano Research2014，7，9： | 10 |
| 4 | SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis显示文摘译解位于下面的机制种回答到不能生活的应力为改进植物压力抵抗是关键的。大部分在 transcriptional 水平响应盐应力对基因表示的规定被知道;然而,很少在 posttranscriptional 水平对这个过程被知道。最近,我们证明那个翻斗车是与钟基因 pre-mRNAs 交往并且为调整他们拼接的选择和 mRNA 成熟是必要的 spliceosome 的一个部件。在这研究,我们发现 skip-1 植物对盐和渗透的压力过分敏感,并且那个翻斗车为拼接的选择和几咸忍耐的基因的 mRNA 成熟被要求,包括 NHX1, CBL1, P5CS1, RCI2A,和 PAT10。染色体宽的分析表明那个翻斗车调停在咸压力的条件下面许多基因拼接的选择,和在 skip-1 的其他的拼接事件的那个大多数包含 intron 保留和罐头 generatea 在抄录 mRNA 的早熟的结束 codon。翻斗车也控制由 modulating 的其他的拼接 5 和 3 的识别或劈开拼接在咸压力的条件下面的施主和领受人地点。因此,这研究探讨的基本问题拼接的 mRNA 在植物的机械在 posttranscriptional 水平贡献咸压力的回答,并且提供在其他的拼接和盐忍耐之间的一个连接。 | Jinlin Feng dingjing Li Zhaoxu Gao Yaru Lu Junya Yu Qian Zheng Shuning Yan Wenjiao Zhang Hang He Ligeng Ma Zhengge Zhu | 2015 | Molecular Plant2015，8，7： | 10 |
| 5 | An expression atlas of miRNAs in Arabidopsis thaliana显示文摘MicroRNAs(miRNAs) are small non-coding RNAs that regulate a variety of biological processes. miRNA expression often exhibits spatial and temporal specificity. However, genome-wide miRNA expression patterns in different organs during development of Arabidopsis thaliana have not yet been systemically investigated. In this study, we sequenced small RNA libraries generated from 27 different organ/tissue types, which cover the entire life cycle of Arabidopsis. Analysis of the sequencing data revealed that most miRNAs are ubiquitously expressed, whereas a small set of miRNAs display highly specific expression patterns. In addition, different miRNA members within the same family have distinct spatial and temporal expression patterns. Moreover, we found that some miRNAs are produced from different arms of their hairpin precursors at different developmental stages. This work provides new insights into the regulation of miRNA biogenesis and a rich resource for future investigation of miRNA functions in Arabidopsis. | Le Xu Yugang Hu Ying Cao Jingrui Li Ligeng Ma Yan Li Yijun Qi | 2018 | Science China(Life Sciences)2018，61，2： | 8 |
| 6 | A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis显示文摘Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature. | Xiaohan Wang Changxin Feng LiLi Tian Congcong Hou Wang Tian Bin Hu Qian Zhang Zhijie Ren Qi Niu Jiali Song Dongdong Kong Liangyu Liu Yikun He Ligeng Ma Chengcai Chu Sheng Luan Legong Li | 2021 | Molecular Plant2021，14，5： | 5 |
| 7 | Brassinosteroid signal trans-duction: An emerging picture显示文摘Steroid hormones play essential roles in animal growth and development. Steroid signaling in animal system is focused on the direct gene regulation response mediated by its nuclear receptors. Recently, steroid hormones are also found in plants. Identification of BRI1 — a critical compo-nent of the plasma-membrane steroid receptor complex, and the related signal transduction pathway mediated by the membrane receptor have revealed an elementary picture of BR signaling from the cell surface perception to the activa-tion of BR-responsive nuclear genes. | WANG Qiaomei1 & MA Ligeng2 1. Department of Horticultural Science, Zhejiang University, Hangzhou 310029, China 2. Department of Biology, Hebei Normal University, Shijiazhuang 050016, China Correspondence should be addressed to Ma Ligeng (e-mail: ligeng.ma @yale.edu) | 2003 | Chinese Science Bulletin2003，48，18： | 3 |
| 8 | Extracellular calmodulin accelerates rbcS-GUS expression in suspension-cultured transgenic tobacco cell显示文摘The role of extracellular calmodulin in regulating expression of rbcS in darkness was examined. A suspension-cultured cell line was generated from the transgenic rbcS-GUS tobacco. It was demonstrated that purified calmodulin added to the media enhanced rbcS-GUS expression. The time course of expression of rbcS-GUS and that of the secretion of calmodulin in the suspended transgenic tobacco cells in darkness were very similar. Both showed initial increase followed by decline with maximum calmodulin secretion preceding maximum GUS expression. The addition of membrane-impermeable calmodulin antagonist W7-agarose inhibited the expression of rbcS-GUS in darkness, but this inhibitory effect was completely reversed by adding exogenous purified calmodulin. These results provide the first evidence that extracellular calmodulin accelerates rbcS gene expression. | MA Ligeng ZHOU Junli ZHANG Suqiao LIU Qiang SUN Daye | 2000 | Chinese Science Bulletin2000，45，22： | 3 |
| 9 | Effects of extracellular calmodulin on pollen germination and tube growth显示文摘The effects of calmodulin (CaM) antagonist W7_agarose, anti_CaM serum and exogenous purified CaM on pollen germination and tube growth of Forsythia suspensa were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7_agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7_agarose and anti_CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7_agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes. | Ligeng Ma Xiaodong Xu Sujuan Cui Daye Sun | 1998 | Chinese Science Bulletin1998，43，2： | 3 |
| 10 | Generation of a series of mutant lines resistant to imidazolinone by screening an EMS-based mutant library in common wheat显示文摘The breeding of herbicide-resistant wheat varieties has helped control weeds in wheat fields economically and effectively.Imidazolinone (IMI) herbicides are popular as they have low toxicity in mammals,are effective at small doses,and exhibit broad-spectrum herbicidal action in the field.Therefore,the isolation and genetic and molecular characterization of IMI-resistant wheat mutants will enhance weed management in wheat fields.In the present study,352 IMI-resistant plants were isolated by genetic screening from a mutant pool prepared by EMS-based random mutagenesis.Cloning of the mutated genes from the IMI-resistant plants indicated that ten taals alleles had been isolated,and mutation in one of three Ta ALS homolog genes conferred IMI resistance,and such a mutation is a dominant trait.Further analysis showed that taals-d exhibited the greatest IMI resistance,whereas taals-b exhibited the weakest resistance to IMI among three homologous taals mutants.In terms of IMI resistance,the taals triple mutant was stronger than the taals double mutants,and the taals double mutants were stronger than the single mutants,indicating a dose-dependent effect of the Ta ALS mutation on IMI resistance in wheat.Biochemical analysis indicated that the mutation in Ta ALS increased the tolerance of Ta ALS to inhibition by IMI.Our work details the genetic and molecular characterization of als wheat mutants,provides a foundation for understanding IMI resistance and breeding wheat varieties with herbicide resistance,and indicates that genetic screening using a mutagenized pool is an effective and important means of breeding crops with additional desired agricultural traits. | Zhuo Chen Zheng Wang Yanfang Heng Jian Li Jiawei Pei Ying Cao Xing Wang Deng Ligeng Ma | 2021 | The Crop Journal2021，9，5： | 3 |
| 11 | Recent advances in CRISPR/Cas9 and applications for wheat functional genomics and breeding显示文摘Common wheat(Triticum aestivum L.)is one of the three major food crops in the world;thus,wheat breeding programs are important for world food security.Characterizing the genes that control important agronomic traits and finding new ways to alter them are necessary to improve wheat breeding.Functional genomics and breeding in polyploid wheat has been greatly accelerated by the advent of several powerful tools,especially CRISPR/Cas9 genome editing technology,which allows multiplex genome engineering.Here,we describe the development of CRISPR/Cas9,which has revo-lutionized the field of genome editing.In addition,we emphasize technological breakthroughs(e.g.base editing and prime editing)based on CRISPR/Cas9.We also summarize recent applications and advances in the functional annotation and breeding of wheat,and we introduce the production of CRISPR-edited DNA-free wheat.Combined with other achievements,CRISPR and CRISPR-based genome editing will speed progress in wheat biology and promote sustainable agriculture. | Jun Li Yan Li Ligeng Ma | 2021 | aBIOTECH2021，2，4： | 3 |
| 12 | Current strategies and advances in wheat biology显示文摘The characterization of agronomically important genes has great potential for the improvement of wheat.However,progress in wheat genetics and functional genomics has been impeded by the high complexity and enormous size of the wheat genome.Recent advances in genome sequencing and sequence assembly have produced a high-quality genome sequence for wheat.Here,we suggest that the strategies used to characterize biological mechanisms in model species,including mutant preparation and characterization,gene cloning methods,and improved transgenic technology,can be applied to wheat biology.These strategies will accelerate progress in wheat biology and promote wheat breeding program development.We also outline recent advances in wheat functional genomics.Finally,we discuss the future of wheat functional genomics and the rational design-based molecular breeding of new wheat varieties to contribute to world food security. | Jun Li Jing Yang Yan Li Ligeng Ma | 2020 | The Crop Journal2020，8，6： | 2 |
| 13 | STABILITY OF SOLUTIONS TO CERTAIN FOURTH-ORDER DELAY DIFFERENTIAL EQUATIONS显示文摘By the Lyapunov functional approach, some better results on the asymptotic stabiBy the Lyapunov functional approach, some better results on the asymptotic stability and global asymptotic stability of zero solution to a certain fourth-order non-linear differential equation with delay are obtained. | Huiyan Kang (School of Math. and Physics, Changzhou University, Changzhou 213016, Jiangsu) Ligeng Si (Dept. of Math., Inner Mongolia Normal University, Huhhot 010020) | 2010 | Annals of Differential Equations2010，26，4： | 2 |
| 14 | Recent advances in the development of nanomaterials for DC-based immunotherapy显示文摘As professional antigen presenting cells, dendritic cells(DCs) greatly determine the quality of the innate and adaptive immunities. Therefore, DC-based immunotherapy has been one of the hotspots in cancer immunotherapy in recent years. Although this unique therapeutic strategy has been approved by U.S. Food and Drug Administration for prostate cancer treatment, the efficacy of DC-based immunotherapy remains to be further improved. Moreover, it is still not completely clear about the immunological basis of DCs, which is another hurdle for the progress of DC-based immunotherapy. Due to their unique physicochemical properties, nanomaterials have shown potentials in addressing these above mentioned problems and have provided important guidelines for optimizing DC-based immunotherapy. However, it is still at the starting stage for this emerging field and there are many critical questions in the rational design of this therapeutic strategy to be answered. Therefore, it is greatly necessary to review and analyze recent progresses in this field. In this review, we mainly focus on the development of various types nanoparticles for DC-based immunotherapy. The existed challenges in this field are also discussed. | Ligeng Xu Jian Xiang Rui Peng Zhuang Liu | 2016 | Science Bulletin2016，61，7： | 2 |
| 15 | The SNW Domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Pall Complex in Arabidopsis显示文摘翻斗车是从酵母的保存蛋白质到植物和人。在工厂房间,翻斗车是由由与 Paf1 建筑群交往集成到 spliceosome 并且作为 transcriptional 使活跃之物作为一个拼接的因素在原子核工作的一个 bifunctional 管理者。在这研究,我们在翻斗车识别了二个原子本地化信号并且证实那各对足够目标翻斗车到原子核。翻斗车的 SNW 域被由与 Paf1 建筑群控制它的相互作用参予 flowering 作为 transcriptional 使活跃之物响应应力,和它的函数支持集成进 spliceosome 作为一个拼接的因素为它的函数要求。包括了翻斗车的 SNW 领域和 N 终点或 C 终点的截断的蛋白质仍然能在基因在 Arabidopsis 拼接和 transcriptional 激活执行全身的蛋白质的功能。另外,我们发现那个翻斗车经历 26S 调停 proteasome 的降级,并且翻斗车的 C 终点被要求在工厂房间维持蛋白质的稳定性。一起,我们的调查结果表明翻斗车的结构的域组织并且在工厂揭示核心域和主题位于 \O 下面翻斗车功能。 | Yan Li Con qconcl Xia Jinlin Feng Dong Yang Fangming Wu Ying Cao Legong Li Ligeng Ma | 2016 | Molecular Plant2016，9，7： | 2 |
| 16 | Characterizing physiological N-use efficiency as influenced by nitrogen management in three rice cultivars显示文摘 | Ligeng Jiang Tingbo Dai Dong Jiang Weixing Cao Xiuqin Gan Shanqing Wei | 2004 | Field Crops Research2004，，2： | 2 |
| 17 | Direct interaction of A rabidopsis cryptochromes with COP1 in light control development显示文摘 | WANG Haiyang MA Ligeng LI Jinming et d | 2001 | Science2001，294，5540： | 1 |
| 18 | Effects of Maillard reaction conditions on the antigenicity of a-lactalbumin and b-lactoglobulin in whey protein conjugated with maltose 显示文摘 | Zheng Li Yongkang Luo Ligeng Feng | 2011 | Eur Food Res Technol2011，233，： | 1 |
| 19 | Postmortem Changes in Physicochemical Properties of Songpu Mirror Carp ( Cyprinus Carpio ) during Iced Storage显示文摘 | Sichao Zhu Zhongyun Zhou Ligeng Feng Yongkang Luo | 2014 | Food Bioscience2014，，： | 1 |
| 20 | Group interactionanalysis in dynamic context 显示文摘 | Dai Peng Di Huijun Dong Ligeng | 2008 | IEEE Trans on Systems Man and Cybernetics Part B: Cybernetics2008，38，1： | 1 |
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