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| 1 | Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients显示文摘B 房间对再感染在肝炎 B 病毒(HBV ) 和保护的清理起一个重要作用。然而,与长期的 HBV 感染的结果被联系的这些房间的功能的特征仍然保持未知。我们包括地在不同阶段从 CHB 病人调查了外部 B 房间子集的频率,显型,和功能:有免疫力的忍耐(信息技术) ,有免疫力的激活(IA ) ,有免疫力的清理(IC ) ,有 HBsAg seroconversion 的应答者(解决的病人, RP ) ,并且健康控制(HC ) 。IA 病人与另外的组相比显示了外部血记忆 B 房间的更低的百分比。B 房间的全面 polyclonal 激活,由激活标记和 IgG 和 IgM 的分泌物的高水平显示了,在 IA 病人被观察。这 B 房间 hyperactivation 能被增加的 IFN-α 导致;并且在 IA 病人的可溶的 CD40 ligands。尤其是,合作激发器分子 CD80 和浆液 HBsAb 的表示和 HBsAg 特定的 B 房间的频率显著地与 HC 题目相比在信息技术, IA,和 IC 病人被减少。更重要地, B 房间 hyperactivation, co-stimulatory 分子 downregulation 和 HBsAg 特定的 B 房间缺陷在 RP 病人被颠倒。B 房间 hyperactivation 和功能的缺陷的颠倒在长期的肝炎 B 病人与 HBsAg seroconversion 被联系。 | Xiangsheng Xu Qinghua Shang Xinyue Chen Weimin Nie Zhengsheng Zou Ang Huang Ming Meng Lei Jin Ruonan Xu Ji-Yuan Zhang Junliang Fu Lifeng Wang Zirong Tang Yunbo Xie Xiaoming Yang Zheng Zhang Fu-Sheng Wang | 2015 | Cellular & Molecular Immunology2015，12，3： | 15 |
| 2 | Study on multi-size seed-metering device for vertical plate soybean precision planter显示文摘Aiming to solve the problem of small range of the appropriately sowing seeds existing in a vertical disc seed-metering device,the planter plate series with four sizes were developed according to the variety and size distribution of all soybeans in China.The structure and working principle of the vertical disc soybean seed-metering device were detailed,and the influence of the diameter of soybean on the working performance of the seed-metering device was analyzed through the software EDEM virtual simulation,so as to achieve the goal of covering the soybean seeds with all sizes by the minimum planter plate series as well as to obtain the most appropriate operating speed of each planter plate by optimization.For the planter plates with the hole diameter of 7,9,12,16 mm,the appropriate size ranges of sowing seeds are 4.5-6.0,6.0-8.0,8.0-10.5,10.5-13.0 mm,respectively,and the appropriate operating speeds are 9,8,7,6 km/h,respectively.The results show that this planter plate series can meet the requirements of seeding with all sizes of soybeans at the range of the most appropriate operating speed.The study method can provide a reference for design and optimization of precision planters. | Liu Hongxin Guo Lifeng Fu Lulu Tang Shifa | 2015 | International Journal of Agricultural and Biological Engineering2015，8，1： | 13 |
| 3 | Wheat genomic study for genetic improvement of traits in China显示文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world. | Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong | 2022 | Science China(Life Sciences)2022，65，9： | 9 |
| 4 | Study on hydrometallurgical process and kinetics of manganese extraction from low-grade manganese carbonate ores显示文摘In order to utilize low-grade manganese ore resources effectively, a hydrometallurgical process was developed for manganese extraction in dilute sulfuric acid medium, and the kinetics of leaching manganese was also investigated. At room temperature, manganese from low-grade manganese carbonate ores was extracted by sulfuric acid leaching without reductants. During the extracting process, single-factor analysis method was used to evaluate the effects of grinding fineness, sulfuric acid concentration,liquid-to-solid ratio, agitation rate and leaching time on the leaching efficiencies of Mn and Fe. The optimal leaching conditions are determined as coarse particles of below 2 mm size(without ball-milling),sulfuric acid concentration of 0.86 mol/L, liquid-to-solid ratio of 5:1, agitation rate of 150 r/min and leaching for 180 min at room temperature. Under the optimal conditions, the leaching efficiencies of Mn and Fe are 96.21% and 13.35%, respectively. In addition, through the experiments at different temperatures, it is found that the leaching process follows the shrinking core model under the conditions of changing acid concentration and intermittent reaction device. Moreover, the apparent activations of effective diffusion and chemical reaction in the kinetic model are calculated to be 18.83 and27.15 kJ/mol, respectively. | Liu Youcai Lin Qingquan Li Lifeng Fu Jiangang Zhu Zhongsi Wang Chongqing Qian Dong | 2014 | International Journal of Mining Science and Technology2014，24，4： | 6 |
| 5 | TLR9 agonist enhances radiofrequency ablation-induced CTL responses, leading to the potent inhibition of primary tumor growth and lung metastasis显示文摘Radiofrequency ablation(RFA)is the most common approach to thermal ablation for cancer therapy.Unfortunately,its efficacy is limited by incomplete ablation,and further optimization of RFA is required.Here,we demonstrate that incubation at 65°C triggers more EG7 tumor cell death by necrosis than treatment at 45°C,and the 65°C-treated cells are more effective at inducing antigen-specific CD8^(+)cytotoxic T lymphocyte(CTL)responses after injection in mice than the 45°C-treated ones.Dendritic cells(DCs)that phagocytose 65°C-treated EG7 cells become mature with upregulated MHCII and CD80 expression and are capable of efficiently inducing effector CTLs in mouse tumor models.RFA(65°C)therapy of EG7 tumors induces large areas of tumor necrosis and stimulates CTL responses.This leads to complete regression of small(~100 mm^(3))tumors but fails to suppress the growth of larger(~350 mm^(3))tumors.The administration of the Toll-like receptor-9(TLR9)agonist unmethylated cytosine-phosphorothioate-guanine oligonucleotide(CpG)to DCs phagocytosing 65°C-treated EG7 cells enhances the expression of MHCII and CD40 on DCs as well as DC-induced stimulation of CTL responses.Importantly,the intratumoral administration of CpG following RFA also increases the frequencies of tumor-associated immunogenic CD11b−CD11c^(+)CD103^(+)DC2 and CD11b+F4/80+MHCII+M1 macrophages and increases CD4^(+)and CD8^(+)T-cell tumor infiltration,leading to enhanced CD4^(+)T cell-dependent CTL responses and potent inhibition of primary RFA-treated or distant untreated tumor growth as well as tumor lung metastasis in mice bearing larger tumors.Overall,our data indicate that CpG administration,which enhances RFA-induced CTL responses and ultimately potentiates the inhibition of primary tumor growth and lung metastasis,is a promising strategy for improving RFA treatment,which may assist in optimizing this important cancer therapy. | Aizhang Xu Lifeng Zhang Jingying Yuan Fatma Babikr Andrew Freywald Rajni Chibbar Michael Moser Wenjun Zhang Bing Zhang Zhaoying Fu Jim Xiang | 2019 | Cellular & Molecular Immunology2019，16，10： | 3 |
| 6 | Structural Basis of SARS-CoV-2 Polymerase Inhibition by Favipiravir显示文摘The outbreak of severe acute respiratory syndrome coronavirus 2(SARSCoV-2)has developed into an unprecedented global pandemic.Nucleoside analogs,such as Remdesivir and Favipiravir,can serve as the firstline broad-spectrum antiviral drugs by targeting the viral polymerases.However,the underlying mechanisms for the antiviral efficacies of these drugs are far from well understood.Here,we reveal that Favipiravir,as a pyrazine derivative,could be incorporated into the viral RNA products by mimicking both adenine and guanine nucleotides.This drug thus inhibits viral replication mainly by inducing mutations in progeny RNAs,different from Remdesivir or other RNA-terminating nucleoside analogs that impair the elongation of RNA products.We further determined the cryo-EM structure of Favipiravir bound to the replicating polymerase complex of SARSCoV-2 in the pre-catalytic state.This structure provides a missing snapshot for visualizing the catalysis dynamics of coronavirus polymerase,and reveals an unexpected base-pairing pattern between Favipiravir and pyrimidine residues that may explain its capacity for mimicking both adenine and guanine nucleotides.These findings shed light on the mechanism of coronavirus polymerase catalysis and provide a rational basis for developing antiviral drugs to combat the SARS-CoV-2 pandemic. | Qi Peng Ruchao Peng Bin Yuan Min Wang Jingru Zhao Lifeng Fu Jianxun Qi Yi Shi | 2021 | The Innovation2021，2，1： | 1 |
| 7 | Reaction kinetics, thermal properties of tetramethyl biphenyl epoxy resin cured with aromatic diamine显示文摘 | Fu Tiezhn Zhang Gang Zhong Shuangling Zhao Chenga Shao Ke Wang Lifeng Na Hui | 2007 | Journal of Applied Polymer Science2007，105，5： | 1 |
| 8 | Study on hydrometallurgical process and kinetics of manganese extraction from low-grade manganese carbonate ores显示文摘 | Liu Youcai Lin Qingquan Li Lifeng Fu Jiangang Zhu Zhongsi Wang Chongqing Qian Dong | 2013 | International Journal of Mining Science and Technology2013，，： | 1 |
| 9 | Anion-Cation Cooperative Catalysis by Ionic Liquids 显示文摘 | Zhang Lifeng Fu Xianlei Gao Guohua | 2011 | Chem Cat Chem2011，3，8： | 1 |
| 10 | Detection of Spotted Fever Group Rickettsia in Haemaphysalis longicornis From Hebei Province, China显示文摘 | Zou Yaxue Wang Qiuyue Fu Zhixin Liu Pengpeng Jin Hongtao Yang Huanhuan Gao Hongwei Xi Zhang Liu Quan Chen Lifeng | 2011 | EN2011，，5： | 1 |
| 11 | Variable Exponent Spaces of Differential Forms on Riemannian Manifold显示文摘 | Yongqiang Fu Lifeng Guo Alberto Fiorenza | 2012 | Journal of Function Spaces and Applications2012，，： | 1 |
| 12 | 三点弯曲法的PBGA封装实验测试(英文)显示文摘任何电子产品在进入市场之前的可靠性测试是十分重要的环节。为此,在之前的几十年中开发了多种类型的测试方法。一种广泛使用的测试方法便是线路板弯曲测试。进行可靠性测试的一个主要问题就是需要大量的测试样品,这是因为这种测试必须重复进行直到获得一致并满意的测量结果。其测试样品的设计,组装工艺和测试程序需要花费长期的时间。如果可靠性测试结果不满意,测试样品需要重新设计、重新生产、重新测试,这从商业的观点来看成本过高且低效。为简化可靠性测试程序,提出了计算模拟的方法在前期设计阶段来预测印制板组装线的可靠性。在目前的研究中,可靠性测试采用了三点弯曲测试法。实现了有限元模式确认及关于该模式应用的2种情况研究。 | Dennis Lau Y. S. Chan S. W. Ricky Lee Lifeng Fu Yuming Ye Sang Liu | 2007 | 电子工业专用设备2007，36，3： | 1 |
| 13 | A new insight into the adsorption of bovine serum albuminonto porous polyethylene membrane by zeta potential measurements,FT-IRanalyses and AFM observations显示文摘 | Xu Tongwen Fu Rongqiang Yan Lifeng | | 0，，： | 1 |
| 14 | Vibration characteristics of a multi-block high-temperature superconducting maglev system显示文摘The multi-block high-temperature superconducting (HTS) maglev system has more complicated dynamic characteristics than the single-block HTS maglev system. To study its vibration characteristics, we designed a maglev measurement system. The system responses at the excitation frequencies of 2, 3 and 15 Hz were examined. Results show that the responses under excitation frequencies of 2 and 3 Hz include a 6 Hz component, which means that the maglev system is a critical nonlinear system. Moreover, the 6 Hz component is much stronger than the 2 Hz or 3 Hz components in the response spectra. There is the interaction between excitation and response. Under an excitation fre-quency of 15 Hz, intensified low-frequency perturbations were observed. | Zhifang FU Lifeng ZHAO Xing LIANG | 2012 | Journal of Modern Transportation2012，20，2： | 1 |
| 15 | Peptide-drug conjugates(PDCs):a novel trend of research and development on targeted therapy,hype or hope?显示文摘Peptide-drug conjugates(PDCs)are the next generation of targeted therapeutics drug after antibody-drug conjugates(ADCs),with the core benefits of enhanced cellular permeability and improved drug selectivity.Two drugs are now approved for market by US Food and Drug Administration(FDA),and in the last two years,the pharmaceutical companies have been developing PDCs as targeted therapeutic candidates for cancer,coronavirus disease 2019(COVID-19),metabolic diseases,and so on.The therapeutic benefits of PDCs are significant,but poor stability,low bioactivity,long research and development time,and slow clinical development process as therapeutic agents of PDC,how can we design PDCs more effectively and what is the future direction of PDCs?This review summarises the components and functions of PDCs for therapeutic,from drug target screening and PDC design improvement strategies to clinical applications to improve the permeability,targeting,and stability of the various components of PDCs.This holds great promise for the future of PDCs,such as bicyclic peptide-toxin coupling or supramolecular nanostructures for peptide-conjugated drugs.The mode of drug delivery is determined according to the PDC design and current clinical trials are summarised.The way is shown for future PDC development. | Chen Fu Lifeng Yu Yuxi Miao Xinli Liu Zhaojin Yu Minjie Wei | 2023 | Acta Pharmaceutica Sinica B2023，13，2： | 1 |
| 16 | Inhibition of phagocytosis reduced the classical activation of BV2 microglia induced by amyloidogenic fragments of beta-amyloid and prion proteins显示文摘在 Alzheimer 的疾病和 prion 疾病的煽动性的回答被 microglia 激活统治。microglial 激活,也就是古典的激活,其他的激活,和获得的释放的三不同显型,被描述了。在这研究,我们调查了 amyloidogenic 碎片的效果淀粉并且 microglial 激活的各种各样的形式上的 prion 蛋白质(A142 和 PrP106126 ) 。我们首先在 cytochalasin D 的 microglial 激活,以及效果的几个标记的 mRNA 表示层次上检验了 A142 和 PrP106126 刺激的效果,一个吞噬作用禁止者,在在刺激 A142 、刺激 PrP106126 的 BV2 microglia 的 microglial 激活上。结果显示出那 A142, PrP106126 导致了 BV2 microglia 的古典激活,减少其他的表示标记的表示水平,并且没在获得的释放标记的表示上有效果。Cytochalasin D 处理显著地减少了 proinflammatory 因素的导致 A142 、导致 PrP106126 的起来规定,但是没改变其他的激活的标记的表示侧面或在暴露于 A142 和 PrP106126 的 BV2 房间获得释放。我们的结果建议 microglia 在细胞外的刺激周围的 microglial 与 amyloidogenic 肽交往古典激活和还原剂它的其他的激活,并且从细胞外的周围的 amyloidogenic 肽的举起放大古典 microglial 激活。 | Fushan Shi Lifeng Yang Jihong Wang Mohammed Kouadir Yang Yang Yongyao Fu Xiangmei Zhou Xiaomin Yin Deming Zhao | 2013 | Acta Biochimica et Biophysica Sinica2013，45，11： | 0 |
| 17 | Structural basis for the inhibition of the SARS-CoV-2 main protease by the anti-HCV drug narlaprevir显示文摘Dear Editor,The second wave of the coronavirus disease(COVID-19)pandemic has recently appeared in Europe.Most European countries,such as France,Germany,and Italy,have announced the implementation of a new round of epidemic prevention and control measures.However,no clinical drug or vaccine has been approved for the treatment of COVID-19.The interim results of the solidarity therapy trial coordinated by the World Health Organization(WHO)indicated that remdesivir,hydroxychloroquine,lopina-vir/ritonavir,and interferon appear to have little or no effect on the 28-day mortality of hospitalized patients or the hospitalization process of new COVID-19 patients.Therefore,there is an urgent need to develop new drugs against COVID-19. | Yu Bai Fei Ye Yong Feng Hanyi Liao Hao Song Jianxun Qi George Fu Gao Wenjie Tan Lifeng Fu Yi Shi | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 0 |
| 18 | Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10,cis-12 CLA on facilitating weaning transition of lambs显示文摘Developing alternatives to antibiotics for prevention of gastrointestinal dysbiosis in early-weaning farmed animals is urgently needed.This study was to explore the potential effects of trans-10,cis-12 conjugated linoleic acid(CLA)on maintaining ruminal homeostasis of young ruminants during the weaning transition period.Thirty neonatal lambs were selected(6 lambs per group)and euthanized for rumen microbial and epithelial analysis.The lambs were weaned at 28 d and experienced the following 5 treatments:euthanized on d 28 as the pre-weaning control(CON0),fed starter feed for 5(CON5)or 21(CON21)d,fed starter feed with 1%of CLA supplemented for 5(CLA5)or 21(CLA21)d.Results showed that the average daily weight gain and dry matter intake were significantly higher in CLA5 than CON5 group.As compared with the CON5 and CON21 group,the relative abundances of volatile fatty acid(VFA)producing bacteria including Bacteroides,Treponema,Parabacteroides and Anaerovibrio,as well as the concentrations of acetate,butyrate and total VFA were significantly increased in CLA5 and CLA21 group,respectively.Integrating microbial profiling and epithelial transcriptome results showed that 7 downregulated inflammatory signaling-related host genes IL2RA,CXCL9,CD4,CCR4,LTB,SPP1,and BCL2A1 with CLA supplementation were significantly negatively correlated with both VFA concentration and VFA producing bacteria,while 3(GPX2,SLC27A2 and ALDH3A1)and 2(GSTM3 and GSTA1)upregulated metabolism-related genes,significantly positively correlated with either VFA concentration or VFA producing bacteria,respectively.To confirm the effects of CLA on epithelial signal transduction,in vitro experiment was further conducted by treating rumen epithelial cells without or with IL-17A+TNF-αfor 12 h after pretreatment of 100μM CLA or not(6 replicates per treatment).The results demonstrated the anti-inflammatory effect of CLA via suppressing the protein expression of NF-кB p-p65/p65 with the activation of peroxisome proliferator-activated receptor gamma(PPARγ).In conclusion,CLA supplementation enhanced the ruminal microbiota-driven transcriptional regulation in healthy rumen epithelial development via rumen VFA production,and CLA may therefore serve as an alternative way to alleviate early-weaning stress and improve physiological and metabolic conditions of young ruminants. | Chunlei Yang Xiangfei Deng Peter Lund Haixia Liu Xingwang Ding Zhengwei Fu Naifeng Zhang Jinjun Li Lifeng Dong | 2023 | Animal Nutrition2023，，1： | 0 |
| 19 | Seismic behavior of precast segmental column bridges under near-fault forward-directivity ground motions显示文摘Precast segmental column bridges exhibit various construction advantages in comparison to traditional monolithic column bridges.However,the lack of cognitions on seismic behaviors has seriously restricted their applications and developments.In this paper,comprehensive investigations are conducted to analyze the dynamic characteristics of precast segmental column bridges under near-fault,forward-directivity ground motions.First,the finite-element models of two comparable bridges with precast segmental columns and monolithic columns are constructed by using OpenSees software,and the nonlinearities of the bridges are considered.Next,three different earthquake loadings are meticulously set up to handle engineering problems,namely recorded near-and far-field ground motions,parameterized pulses,and pulse and residual components extracted from real records.Finally,based on the models and earthquake sets,extensive explorations are carried out.The results show that near-fault forward-directivity ground motions are more threatening than far-field ones;precast segmental column bridges may suffer more pounding impacts than monolithic bridges;the“narrow band”effect caused by near-fault,forward-directivity ground motions may occur in bridges with shorter periods than pulse periods;and pulse and residual components play different roles in seismic responses. | Xin Lifeng Li Xiaozhen Fu Peiyao Mu Di | 2022 | Earthquake Engineering and Engineering Vibration2022，21，2： | 0 |
| 20 | Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids显示文摘Studies of cultured embryos have provided insights into human peri-implantation development.However,detailed knowledge of peri-implantation lineage development as well as underlying mechanisms remains obscure.Using 3D-cultured human embryos,herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives.In addition,we develop an embryo-like assembloid(E-assembloid)by assembling naive hESCs and extraembryonic cells.Using human embryos and E-assembloids,we reveal that WNT,BMP and Nodal signaling pathways synergistically,but functionally differently,orchestrate human peri-implantation lineage development.Specially,we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications.Finally,an improved E-assembloid is developed to recapitulate the epiblast and hypoblast development and tissue architectures in the pregastrulation human embryo.Our findings provide insights into human peri-implantation development,and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis. | Zongyong Ai Baohua Niu Yu Yin Lifeng Xiang Gaohui Shi Kui Duan Sile Wang Yingjie Hu Chi Zhang Chengting Zhang Lujuan Rong Ruize Kong Tingwei Chen Yixin Guo Wanlu Liu Nan Li Shumei Zhao Xiaoqing Zhu Xuancheng Mai Yonggang Li Ze Wu Yi Zheng Jianping Fu Weizhi Ji Tianqing Li | 2023 | Cell Research2023，33，9： | 0 |
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