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| 1 | Efficacy of cattle encephalon glycoside and ignotin in patients with acute cerebral infarction: a randomized, double-blind, parallel-group, placebo-controlled study显示文摘Cattle encephalon glycoside and ignotin(CEGI)injection is a compound preparation formed by a combination of muscle extract from hea lthy rabbits and brain gangliosides from cattle,and it is generally used as a neuroprotectant in the treatment of central and peripheral nerve injuries.However,there is still a need for high-level clinical evidence from large samples to support the use of CEGI.We therefore carried out a prospective,multicenter,randomized,double-blind,parallel-group,placebo-controlled study in which we recruited 319 patients with acute cerebral infarction from 16 centers in China from October 2013 to May 2016.The patients were randomized at a 3:1 ratio into CEGI(n=239;155 male,84 female;61.2±9.2 years old)and placebo(n=80;46 male,34 female;63.2±8.28 years old)groups.All patients were given standard care once daily for 14 days,including a 200 mg aspirin enteric-coated tablet and 20 mg atorvastatin calcium,both taken orally,and intravenous infusion of 250–500 mL 0.9%sodium chloride containing 40 mg sodium tanshinone IIA sulfonate.Based on conventional treatment,patients in the CEGI and placebo groups were given 12 mL CEGI or 12 mL sterile water,respectively,in an intravenous drip of 250 mL 0.9%sodium chloride(2 mL/min)once daily for 14 days.According to baseline National Institutes of Health Stroke Scale scores,patients in the two groups were divided into mild and moderate subgroups.Based on the modified Rankin Scale results,the rate of patients with good outcomes in the CEGI group was higher than that in the placebo group,and the rate of disability in the CEGI group was lower than that in the placebo group on day 90 after treatment.In the CEGI group,neurological deficits were decreased on days 14 and 90 after treatment,as measured by the National Institutes of Health Stroke Scale and the Barthel Index.Subgroup analysis revealed that CEGI led to more significant improvements in moderate stroke patients.No drug-related adverse events occurred in the CEGI or placebo groups.In conclusion,CEGI may be a safe and effective treatment for acute cerebral infarction patients,especially for moderate stroke patients.This study was approved by the Ethical Committee of Peking University Third Hospital,China(approval No.2013-068-2)on May 20,2013,and registered in the Chinese Clinical Trial Registry(registration No.ChiCTR1800017937). | Hui Zhang Chuan-Ling Li Feng Wan Su-Juan Wang Xiu-E Wei Yan-Lei Hao Hui-Lin Leng Jia-Min Li Zhong-Rui Yan Bao-Jun Wang Ren-Shi Xu Ting-Min Yu Li-Chun Zhou Dong-Sheng Fan | 2020 | Neural Regeneration Research2020，15，7： | 54 |
| 2 | Anatomical distribution of pelvic deep infiltrating endometriosis and its relationship with pain symptoms显示文摘背景子宫内膜增生是渗入子宫内膜增生的争论、神秘的 disease.Deep (死)为痛苦的症状负责并且是 endometriosis.Little 工作的最少的理解的类型被奉献了定义地点死有 pain.The 的损害和它的关系学习瞄准是调查在解剖分发之间的关系死损害和疼痛 symptoms.Methods 从在2003年5月和2007年12月与之间的 354 个病人的临床的数据 | Dai Yi Leng Jin-hua Lang Jing-he Li Xiao-yan Zhang Jun-ji | 2012 | Chinese Medical Journal2012，，2： | 43 |
| 3 | Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients显示文摘AIM:To evaluate the diagnostic value of cancer-testis antigen(CTA) mRNA in peripheral blood samples from hepatocellular carcinoma(HCC) patients.METHODS:Peripheral blood samples were taken from 90 patients with HCC before operation.Expression of melanoma antigen-1(MAGE-1),synovial sarcoma X breakpoint-1(SSX-1),and cancer-testis-associated protein of 11 kDa(CTp11) mRNA in peripheral blood mononuclear cells(PBMC) was tested by nested reverse transcriptspolymerase chain reaction(RT-PCR).Serum α-fetoprotein(AFP) in these patients was also determined.RESULTS:The positive rate of MAGE-1,SSX-1 and CTp11 transcripts was 37.7%,34.4%,31.1% in PBMC samples,and 74.4%,73.3%,62.2% in their resected tumor samples,respectively.The positive rate for at least one of the transcripts of three CTA genes was 66.7% in PBMC samples and 91.1% in their resected tumor samples.MAGE-1,SSX-1 and/or CTp11 mRNA were not detected in the PBMC of those patients from whom the resected tumor samples were MAGE-1,SSX-1 and/or CTp11 mRNA negative,nor in the PBMC samples from 20 healthy donors and 10 cirrhotic patients.Among the 90 patients,the serum AFP in 44 patients met the general diagnostic standard(AFP > 400 μg/L) for HCC,and was negative(AFP ≤ 20 μg/L) or positive with a low concentration(20 μg/L < AFP ≤ 400 μg/L) in the other patients.The positive rate for at least one of the transcripts of three CTA genes in PBMC samples from the AFP negative or positive patients with a low concentration was 69.2% and 45.0%,respectively.Of the 90 patients,71(78.9%) were diagnosed as HCC by nested RT-PCR and serum AFP.Although the positive rate for at least one of the transcripts of three CTA genes in PBMC samples from 53 patients at TNM stage or was obviously higher than that in PBMC samples from 37 patients at stage or(77.9% vs 51.4%,P = 0.010),the CTA mRNA was detected in 41.7% and 56.0% of PBMC samples from HCC patients at stages andrespectively.CONCLUSION:Detecting MAGE-1,SSX-1 and CTp11 mRNA in PBMC improves the total diagnostic rate of HCC. | Li Zhao Dong-Cheng Mou Zeng-An Wu Ji-Run Peng Lei Huang Xi-Sheng Leng | 2010 | World Journal of Gastroenterology2010，16，32： | 22 |
| 4 | Total glucosides of paeony attenuates animal psoriasis induced inflammatory response through inhibiting STAT1 and STAT3 phosphorylation显示文摘OBJECTIVE Psoriasis is an immune system meditated disease,especially T cells.It disturbed many people around the world and hard to therapy.Paeonia lactiflora Pall has been used as a medicine in china for thousands of years.Recent studies has found that the main component of Paeonia lactiflora Pall can alleviates the immune response in many diseases.In this study,we researched the effects and possible mechanisms of total glucosides of paeony(TGP)on animal psoriasis in order to study the therapeutic effects and mechanisms of TGP in 5%propranolol creaminduced psoriasis in guinea pigs and Imiquimod(IMQ)cream-induced psoriasis in mice.METHODS The effect of TGP was evaluated using a psoriasis-like model of guinea pigs and mice.Ear thickness was accessed,and pathology injury was observed by HE staining.The levels of serum IL-1β,IL-6,IL-12,IL-17,IL-23,TNF-α,and IFN-γ,skin IL-17A,IL-22 and orphan nuclear receptor(RORγt)mRNA expression,proliferating cell nuclear antigen(PCNA),total or phosphorylated signal transducers and activators of transcription(STAT1 and STAT3)were determined by ELISA,real time PCR,immu⁃nohistochemical staining,and Western blotting,respectively.RESULTS Compared with model group,TGP treatment decreased the ear thickness,improved pathology of psoriasis,alleviated IMQ-induced keratinocyte proliferation,reduced the inflammatory cytokine,and downregulated IL-17A,IL-22,and RORγt mRNA in mice.Further study indicated that TGP inhibited STAT1 and STAT3 phosphorylation in lesion skins of psoriasis-like mice.CONCLUSION TGP alleviates the symptoms of psoriasis-like guinea pigs and mice,and the possible mechanism may relate to inhibit T helper 17(TH17)cell differentiation and keratinocytes proliferation by inhibiting STAT1 and STAT3 phosphorylation. | LI Bin-bin HE Shu-cheng LIU Rui HUANG Liang-liang LIU Ge WANG Rui-xuan YANG Zhuo-yue LIU Xin-yi LENG Ye LIU Dan YE Cheng-yu LI Yun-man CHEN Yong-jian IN Hong FANG Wei-rong | 2019 | 中国药理学与毒理学杂志2019，33，9： | 21 |
| 5 | Thermal energy storage:Challenges and the role of particle technology显示文摘Thermal energy is at the heart of the whole energy chain providing a main linkage between the primary and secondary energy sources.Thermal energy storage(TES)has a pivotal role to play in the energy chain and hence in future low carbon economy.However,a competitive TES technology requires a number of scientific and technological challenges to be addressed including TES materials,TES components and devices,and integration of TES devices with energy networks and associated dynamic optimization.This paper provides a perspective of TES technology with a focus on TES materials challenges using molten salts based phase change materials for medium and high temperature applications.Two key challenges for the molten salt based TES materials are chemical incompatibility and low thermal conductivity.The use of composite materials provides an avenue to meeting the challenges.Such composite materials consist of a phase change material,a structural supporting material,and a thermal conductivity enhancement material.The properties of the supporting material could determine the dispersion of the thermal conductivity enhancement material in the salt.A right combination of the salt,the structural supporting material,and the thermal conductivity enhancement material could give a hierarchical structure that is able to encapsulate the molten salt and give a substantial enhancement in the thermal conductivity.Understanding of the structure-property relationships for the composite is essential for the formulation design and fabrication of the composite materials.Linking materials properties to the system level performance is recommended as a key future direction of research. | Zhiwei Ge Yongliang Li Dacheng Li Ze Sun Yi Jin Chuanping Liu Chuan Li Guanghui Leng Yulong Ding | 2014 | Particuology2014，12，4： | 20 |
| 6 | Age-specific prevalence of human papillomavirus by grade of cervical cytology in Tibetan women显示文摘背景人的乳头状瘤病毒(HPV ) 被相信是最普通的性播送的感染。然而,很少在中国对 HPV 类型的流行和分发被知道。我们试图在居住在 China.Methods A 的西藏的自治区域的女人为反常颈的细胞学象风险一样估计流行和 HPV 类型的分发代表性的学习被执行包含 3036 个女人的一件样品。一张流行病学的问询表被使用,颈的标本为基于液体的细胞学和 HPV DNA 察觉被获得。统计分析在这张人口包括了 chi 平方和逻辑回归 model.Results,(111/3036 )3.66% 有未经决定的意义(子囊) 的不正常的有鳞的房间, 1.45%(44/3036 ) 低档有鳞的 intraepithelial 损害(LSIL ) 并且(33/3036 )1.09% 有高级有鳞的 intraepithelial 损害(HSIL ) 。西藏的女人(5.74% , 137/2387 ) 展出更低的反常细胞学比非西藏的女人评价(8.01% , 52/649, P=0.03 ) 。HPV 感染的全面流行是 9.19%(279/3036 ) 。我们没能到年龄在 HPV 流行识别任何差别。在有正常,子囊, LSIL 和 HSIL 的组,全面 HPV 流行是 7.41%(211/2847 ) , 24.32%(27/111 ) , 56.82%(25/44 ) 并且 45.45%(15/33 ) 分别地。HPV 16 (1.52%,46/3036 ) 是最普通的类型,并且在有子囊的女人也是最流行的(8.11% , 9/111 ) 并且 HSIL (15.15% , 5/33 ) 。为西藏的女人的最普通的 HPV 类型是 HPV 16 (1.42% , 34/2387 ) 而为非西藏的个人它是 HPV 33 (2.31% , 15/649 ) 。279 个感染 HPV 的女人, 40 个个人(14.34%) 与多重 HPV 确实介绍了。有二怀孕的女人是更可能的有反常细胞学油迹(OR=1.67; 95% CI:1.07-2.61 ).Conclusions HPV 确实的低流行在居住在中国的西藏的自治区域的女人被观察。反常颈的细胞学和 HPV 类型分布的流行在西藏、非西藏的女人之间是不同的。 | JIN Qiong SHEN Keng LI Hui ZHOU Xian-rong HUANG Hui-fang LENG Jin-hua | 2010 | Chinese Medical Journal2010，，15： | 19 |
| 7 | dentification of Circular RNAs as a Novel Biomarker for Ovarian Endometriosis显示文摘 | Xiao-Xuan Xu Shuang-Zheng Jia Yi Dai Jun-Ji Zhang Xiao-Yan Li Jing-Hua Shi Jin-Hua Leng Jing-He Lang | 2018 | Chinese Medical Journal2018，，5： | 14 |
| 8 | Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene encoding a key enzyme during abscisic acid (ABA) biosynthesis and ABA-regulated ethylene production in detached young persimmon calyx显示文摘Unlike the typical climacteric fruits, persimmons (Diospyros kaki Thunb.) produce higher levels of ethylene when they are detached from trees at a younger stage. In order to obtain detailed information on the role of abscisic acid (ABA) in ripening, we cloned the DKNCED1, DKACS2, and DKACO1 genes from the calyx. Water loss was first noted in the calyx lobe, and DKNCED1 was highly expressed 1 d after the fruits were detached, coinciding with an increase in the ABA content. Then, the DKACS2 and DKACO1 genes were expressed after some delay. In the calyx, the ABA peak was observed 2 d after the fruits were harvested, and this peak preceded the ethylene peak observed on day 3. The fruit firmness rapidly decreased on day 4, and the fruits softened completely 6 d after they were harvested. The increases in the expressions of ABA, ethylene, and the genes in the calyxes occurred earlier than the corresponding increases in the pulp, although the 3 increases occurred on different days. Exogenous ABA treatment increased ABA concentration, induced expression of both ACS and ACO, and promoted ethylene synthesis and young-fruit softening; by contrast, treatment with NDGA inhibited the gene expressions and ethylene synthesis and delayed young-fruit softening. These results indicate that ethylene biosynthesis in the detached young persimmon fruits is initially triggered by ABA, which is induced by water loss in the calyx, through the induction of DKACS2 and DKACO1 expressions. The ethylene produced in the calyx subsequently diffuses into the pulp tissue, where it induces autocatalytic ethylene biosynthesis, resulting in an abrupt increase in ethylene production. | LENG Ping ZHANG GuangLian LI XiangXin WANG LiangHe ZHENG ZhongMing | 2009 | Chinese Science Bulletin2009，54，16： | 13 |
| 9 | Association of biochar properties with changes in soil bacterial,fungal and fauna communities and nutrient cycling processes显示文摘Soil microorganisms play crucial roles in soil nutrient cycling,carbon sequestration,fertility maintenance and crop health and production.To date,the responses of microorganisms,such as microbial activity,diversity,community structure and nutrient cycling processes,to biochar addition have been widely reported.However,the relationships between soil microbial groups(bacteria,fungi and microscopic fauna)and biochar physicochemical properties have not been summarized.In this review,we conclude that biochar affects soil microbial growth,diversity and community compositions by directly provid-ing growth promoters for soil biota or indirectly changing soil basic properties.The porous structure,labile C,high pH and electrochemical properties of biochar play an important role in determining soil microbial abundance and communities,and their mediated N and P cycling processes,while the effects and underlying mechanisms vary with biochar types that are affected by pyrolysis temperature and feedstock type.Finally,we highlight some issues related to research methodology and subjects that are still poorly understood or controversial,and the perspectives for further research in microbial responses to biochar addition. | Zhongmin Dai Xinquan Xiong Hang Zhu Haojie Xu Peng Leng Jihui Li CTang Jianming Xu | 2021 | Biochar2021，3，3： | 12 |
| 10 | Diagnostic value of elevated serum carbohydrate antigen 199 level in acute cholangitis secondary to choledocholithiasis显示文摘AIM To investigate the diagnostic value of abnormal serum carbohydrate antigen 199(CA199) level in acute cholangitis secondary to choledocholithiasis.METHODS In this retrospective cohort study, the clinical data of 727 patients with choledocholithiasis admitted to the Third Affiliated Hospital of Zunyi Medical College from June 2011 to June 2017 were collected. Among these patients, 258 patients had secondary acute cholangitis and served as observation group, and the remaining 569 choledocholithiasis patients served as the control group. Serum liver function indexes and tumor markers were detected in both groups, and the receiver operating characteristic(ROC) curves were constructed for markers showing statistical significances. The cutoff value, sensitivity, and specificity of each marker were calculated according to the ROC curves. RESULTS The results of liver function tests showed no significant differences between the two groups(P > 0.05). Tumor markers including serum CA125, CA153, carcinoembryonic antigen, and alpha fetoprotein levels were also not significantly different(P > 0.05); however, the serum CA199 level was significantly higher in the observation group than in the control group(P < 0.05). The ROC curve analysis showed that the area under the curve was 0.885(95%CI: 0.841-0.929) for CA199, and the cutoff value of 52.5 kU/L had the highest diagnostic accuracy, with a sensitivity of 86.8% and a specificity of 81.6%.CONCLUSION Abnormally elevated serum CA199 level has an important value in the diagnosis of acute cholangitis secondary to choledocholithiasis. It may be a specific inflammatory marker for acute cholangitis. | Yong Mei Li Chen Ci-Jun Peng Jun Wang Peng-Fei Zeng Guo-Xing Wang Wen-Ping Li Yan-Qing Luo Chao Du Kai Liu Kun Xiong Kai Leng Chun-Lin Feng Ji-Hu Jia | 2018 | World Journal of Clinical Cases2018，6，11： | 11 |
| 11 | Menorrhagia and Uterine Volume Associated with Lower Urinary Tract Symptoms in Patients with Adenomyosis显示文摘 | Ting Li Xiao-Xuan Xu Yi Dai Jun-Ji Zhang Jing-He Lang Jin-Hua Leng | 2017 | Chinese Medical Journal2017，，13： | 10 |
| 12 | Total polysaccharides of the Sijunzi decoction attenuate tumor necrosis factor-α-induced damage to the barrier function of a Caco-2 cell monolayer via the nuclear factor-κB-myosin light chain kinase-myosin light chain pathway显示文摘AIM To explore the protective effects and underlying mechanisms of total polysaccharides of the Sijunzi decoction(TPSJ) on the epithelial barriers in vitro. METHODS Caco-2 cell monolayers were treated with or without TPSJ in the presence or absence of TNF-α, and paracellular permeability and transepithelial electrical resistance(TEER) were measured to evaluate the epithelial barrier function. Immunofluorescence and western blotting were respecti-vely used to evaluate the distribution and expression of the tight junction proteins claudin 1, claudin 2, zo3, and occludin in Caco-2 cells. western blotting was also used to evaluate the cellular expression of myosin light chain(MLC), phosphorylated MLC(pM LC), MLC kinase(MLCK), and nuclear factor(NF)-κB p65. RESULTS TPSJ promoted the proliferation of Caco-2 cells and inhibited TNF-α-induced secretion of pro-inflammatory cyto-kines. Furthermore, TPSJ significantly ameliorated both the reduction of TEER and the increased paracellular permeability observed in tumor necrosis factor(TNF)-α-damaged Caco-2 monolayers. Furthermore, TPSJ remarkably attenuated TNF-α-induced morphological changes, downregulated the expression of claudin 1, claudin 2, zo3, and occludin, and markedly suppressed TNF-α-mediated upregulation of p-MLC and MLCK expression. Finally, TPSJ inhibited the activation and expression of NF-κB p65. CONCLUSION Our results demonstrate that TPSJ alleviates the TNF-α-induced impairment of the intestinal epithelial cell barrier function by suppressing NF-κB p65-mediated phosphorylation of MLCK and MLC. | Yue Lu Leng Li Jin-wei Zhang Xiao-qin Zhong Jian-An wei Ling Han | 2018 | World Journal of Gastroenterology2018，24，26： | 10 |
| 13 | Inequalities for polars of mixed projection bodies显示文摘In 1993 Lutwak established some analogs of the Brunn-Minkowsi inequality and the Aleksandrov-Fenchel inequality for mixed projection bodies. In this paper, following Lutwak, we give their polars forms. Further, as applications of our methods, we give a generalization of Pythagorean inequality for mixed volumes. | LENG Gangsong, ZHAO Changjian, HE Binwu & LI XiaoyanDepartment of Mathematics, Shanghai University, Shanghai 200436, China Department of Mathematics, Binzhou Teachers College, Binzhou 256604, China | 2004 | Science China Mathematics2004，47，2： | 9 |
| 14 | Aspirin inhibits proliferation of gemcitabine-resistant human pancreatic cancer cells and augments gemcitabine-induced cytotoxicity显示文摘 | Yan-qiu OU Wen bo ZHU Yan LI Peng-xin QIU Yi-jun HUANG Jun XIE Song-min HE Xiao-ke ZHENG Tian-dong LENG Dong XU Guang-mei YAN | 2010 | Acta Pharmacologica Sinica2010，31，1： | 9 |
| 15 | Three-dimensional-printed individualized porous implants:A new“implant-bone”interface fusion concept for large bone defect treatment显示文摘Bone defect repairs are based on bone graft fusion or replacement.Current large bone defect treatments are inadequate and lack of reliable technology.Therefore,we aimed to investigate a simple technique using three-dimensional(3D)-printed individualized porous implants without any bone grafts,osteoinductive agents,or surface biofunctionalization to treat large bone defects,and systematically study its long-term therapeutic effects and osseointegration characteristics.Twenty-six patients with large bone defects caused by tumor,infection,or trauma received treatment with individualized porous implants;among them,three typical cases underwent a detailed study.Additionally,a large segmental femur defect sheep model was used to study the osseointegration characteristics.Immediate and long-term biomechanical stability was achieved,and the animal study revealed that the bone grew into the pores with gradual remodeling,resulting in a long-term mechanically stable implant-bone complex.Advantages of 3D-printed microporous implants for the repair of bone defects included 1)that the stabilization devices were immediately designed and constructed to achieve early postoperative mobility,and 2)that osseointegration between the host bone and implants was achieved without bone grafting.Our osseointegration method,in which the“implant-bone”interface fusion concept was used instead of“bone-bone”fusion,subverts the traditional idea of osseointegration. | Teng Zhang Qingguang Wei Hua Zhou Zehao Jing Xiaoguang Liu Yufeng Zheng Hong Cai Feng Wei Liang Jiang Miao Yu Yan Cheng Daoyang Fan Wenhao Zhou Xinhong Lin Huijie Leng Jian Li Xinyu Li Caimei Wang Yun Tian Zhongjun Liu | 2021 | Bioactive Materials2021，6，11： | 9 |
| 16 | The status of exploitation techniques of natural gas hydrate显示文摘Natural gas hydrate(NGH)has been widely considered as an alternative form of energy with huge potential,due to its tremendous reserves,cleanness and high energy density.Several countries involving Japan,Canada,India and China have launched national projects on the exploration and exploitation of gas hydrate resources.At the beginning of this century,an early trial production of hydrate resources was carried out in Mallik permafrost region,Canada.Japan has conducted the first field test from marine hydrates in 2013,followed by another trial in 2017.China also made its first trial production from marine hydrate sediments in 2017.Yet the low production efficiency,ice/hydrate regeneration,and sand problems are still commonly encountered;the worldwide progress is far before commercialization.Up to now,many gas production techniques have been proposed,and a few of them have been adopted in the field production tests.Nevertheless,hardly any method appears really promising;each of them shows limitations at certain conditions.Therefore,further efforts should be made on the economic efficiency as well as sustainability and environmental impacts.In this paper,the investigations on NGH exploitation techniques are comprehensively reviewed,involving depressurization,thermal stimulation,chemical inhibitor injection,CO2–CH4 exchange,their combinations,and some novel techniques.The behavior of each method and its further potential in the field test are discussed.The advantages and limitations of laboratory studies are also analyzed.The work could give some guidance in the future formulation of exploitation scheme and evaluation of gas production behavior from hydrate reservoirs. | Lei Yang Yulong Liu Hanquan Zhang Bo Xiao Xianwei Guo Rupeng Wei Lei Xu Lingjie Sun Bin Yu Shudong Leng Yanghui Li | 2019 | Chinese Journal of Chemical Engineering2019，27，9： | 9 |
| 17 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 18 | Combination of serum gamma-glutamyltransferase and alkaline phosphatase in predicting the diagnosis of asymptomatic choledocholithiasis secondary to cholecystolithiasis显示文摘BACKGROUND Gamma-glutamyltransferase(GGT) is one of the most important laboratory tests for the evaluation of liver damage. Through a long-term clinical observation of patients with secondary asymptomatic choledocholithiasis, we found that most patients had abnormal GGT serum levels.AIM To investigate the combination of serum GGT and alkaline phosphatase(ALP) in predicting the diagnosis of asymptomatic choledocholithiasis secondary to cholecystolithiasis.METHODS In this retrospective cohort study, the clinical data of 829 patients with cholecystolithiasis admitted to the Third Affiliated Hospital of Zunyi Medical College from August 2014 to August 2017 were collected. Among these patients,151 patients had secondary asymptomatic choledocholithiasis and served as the observation group, and the remaining 678 cholecystolithiasis patients served as the control group. Serum liver function indexes were detected in both groups,and the receiver operating characteristic(commonly known as ROC) curves were constructed for markers showing statistical significances. The cutoff value,sensitivity, and specificity of each marker were calculated according to the ROC curves.RESULTS The overall incidence of asymptomatic choledocholithiasis secondary to cholecystolithiasis was 18.2%. The results of liver function indexes including serum aspartate aminotransferase, alanine aminotransferase, direct bilirubin and total bilirubin levels showed no significant differences between the two groups(P> 0.05). However, the serum GGT and ALP levels were significantly higher in the observation group than in the control group(P < 0.05). The ROC curve analysis showed that the area under the curve was 0.881(95%CI: 0.830-0.932), 0.647(95%CI: 0.583-0.711) and 0.923(0.892-0.953) for GGT, ALP, and GGT + ALP,respectively. The corresponding cut-off values of GGT and ALP were 95.5 U/L and 151.5 U/L, sensitivity were 90.8% and 65.1%, and specificity were 83.6% and59.8%, respectively. The sensitivity and specificity of GGT + ALP were 93.5% and85.1%, respectively.CONCLUSION An abnormally elevated serum GGT level has an important value in the diagnosis of asymptomatic choledocholithiasis secondary to cholecystolithiasis.The combination of serum GGT and ALP has better diagnostic performance. As a convenient, rapid and inexpensive test, it should be applied in secondary asymptomatic choledocholithiasis routine screening. | Yong Mei Li Chen Peng-Fei Zeng Ci-Jun Peng Jun Wang Wen-Ping Li Chao Du Kun Xiong Kai Leng Chun-Lin Feng Ji-Hu Jia | 2019 | World Journal of Clinical Cases2019，7，2： | 8 |
| 19 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 20 | LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition显示文摘Despite the structural conservation of PTEN with dual-specificity phosphatases,there have been no reports regarding the regulatory mechanisms that underlie this potential dual-phosphatase activity.Here,we report that K27-linked polyubiquitination of PTEN at lysines 66 and 80 switches its phosphoinositide/protein tyrosine phosphatase activity to protein serine/threonine phosphatase activity.Mechanistically,high glucose,TGF-β,CTGF,SHH,and IL-6 induce the expression of a long non-coding RNA,GAEA(Glucose Aroused for EMT Activation),which associates with an RNA-binding E3 ligase,MEX3C,and enhances its enzymatic activity,leading to the K27-linked polyubiquitination of PTEN.The MEX3C-catalyzed PTEN^K27-polyUb activates its protein serine/threonine phosphatase activity and inhibits its phosphatidylinositol/protein tyrosine phosphatase activity.With this altered enzymatic activity,PTEN^K27-polyUb dephosphorylates the phosphoserine/threonine residues of TWIST1,SNAI1,and YAP1,leading to accumulation of these master regulators of EMT.Animals with genetic inhibition of PTEN^K27-polyUb,by a single nucleotide mutation generated using CRISPR/Cas9(Pten^K80R/K80R),exhibit inhibition of EMT markers during mammary gland morphogenesis in pregnancy/lactation and during cutaneous wound healing processes.Our findings illustrate an unexpected paradigm in which the lncRNA-dependent switch in PTEN protein serine/threonine phosphatase activity is important for physiological homeostasis and disease development. | Qingsong Hu Chunlai Li Shouyu Wang Yajuan Li Bo Wen Yanyan Zhang Ke Liang Jun Yao Youqiong Ye Heidi Hsiao Tina K.Nguyen Peter K.Park Sergey D.Egranov David H.Hawke Jeffrey R.Marks Leng Han Mien-Chie Hung Bing Zhang Chunru Lin Liuqing Yang | 2019 | Cell Research2019，29，4： | 8 |
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