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| 1 | Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene encoding a key enzyme during abscisic acid (ABA) biosynthesis and ABA-regulated ethylene production in detached young persimmon calyx显示文摘Unlike the typical climacteric fruits, persimmons (Diospyros kaki Thunb.) produce higher levels of ethylene when they are detached from trees at a younger stage. In order to obtain detailed information on the role of abscisic acid (ABA) in ripening, we cloned the DKNCED1, DKACS2, and DKACO1 genes from the calyx. Water loss was first noted in the calyx lobe, and DKNCED1 was highly expressed 1 d after the fruits were detached, coinciding with an increase in the ABA content. Then, the DKACS2 and DKACO1 genes were expressed after some delay. In the calyx, the ABA peak was observed 2 d after the fruits were harvested, and this peak preceded the ethylene peak observed on day 3. The fruit firmness rapidly decreased on day 4, and the fruits softened completely 6 d after they were harvested. The increases in the expressions of ABA, ethylene, and the genes in the calyxes occurred earlier than the corresponding increases in the pulp, although the 3 increases occurred on different days. Exogenous ABA treatment increased ABA concentration, induced expression of both ACS and ACO, and promoted ethylene synthesis and young-fruit softening; by contrast, treatment with NDGA inhibited the gene expressions and ethylene synthesis and delayed young-fruit softening. These results indicate that ethylene biosynthesis in the detached young persimmon fruits is initially triggered by ABA, which is induced by water loss in the calyx, through the induction of DKACS2 and DKACO1 expressions. The ethylene produced in the calyx subsequently diffuses into the pulp tissue, where it induces autocatalytic ethylene biosynthesis, resulting in an abrupt increase in ethylene production. | LENG Ping ZHANG GuangLian LI XiangXin WANG LiangHe ZHENG ZhongMing | 2009 | Chinese Science Bulletin2009，54，16： | 13 |
| 2 | Percutaneous extraction of leads from coronary sinus vein and branch by modified techniques显示文摘 | CHU Xian-ming LI Xue-bin ZHANG Ping, WANG Long LI Ding LI Bing AN Yi LENG Min DUAN Jiang-bo GUO Ji-hong | 2012 | Chinese Medical Journal2012，，20： | 6 |
| 3 | A remote sensing monitoring method for alpine grasslands desertification in the eastern Qinghai–Tibetan Plateau显示文摘Alpine grassland is the typical vegetation in the eastern Qinghai–Tibetan Plateau,which has important ecological service functions,and also supports the development of alpine stock farming.In recent years,under both the natural and human disturbance,alpine grasslands in this area have appeared to different degrees of desertification.A diagnosis of the desertification degree serves as the basis for grassland ecological restoration.This study constructs a comprehensive index based on remote sensing called alpine grassland desertification index(AGDI)to monitor the areas and degree of desertification.The most relevant indicators of desertification,namely,vegetation fraction,aboveground biomass,soil moisture,and land surface temperature,were selected to establish AGDI.The geographical detector is used to reselect and assess these indicators.The results show that the overall verification accuracy of AGDI is 82.05%.In particular,the accuracy of identifying severe desertification is the highest.Our study confirms that the desertification of alpine grasslands in the eastern Qinghai–Tibetan Plateau is characterized by fragmentation.Thus,Landsat-8 OLI data with a spatial resolution of 30 m is more suitable than MODIS data for alpine grasslands desertification monitoring.The research results can provide a methodological reference for monitoring desertification of alpine grasslands and other grassland regions in the world. | KUANG Qian YUAN Quan-zhi HAN Ji-chong LENG Rong WANG Yu-shuang ZHU Ke-hong LIN Shuo REN Ping | 2020 | Journal of Mountain Science2020，17，6： | 4 |
| 4 | Clinical analysis of laparoscopic surgery for ovarian masses under different conditions during the second trimester显示文摘 | PENG Ping ZHU Lan LANG Jing-he LIU Zhu-feng SUN Da-wei LENG Jin-hua | 2013 | Chinese Medical Journal2013，，17： | 3 |
| 5 | PARAMETRIC APPROACH TO DESIGN OF HULL FORMS显示文摘The parametric approach to design of hull form was studied in this work, which provides the means for quick generation and variation of hull form for the hydrodynamic optimization of hull form. | ZHANG Ping ZHU De-xiang LENG Wen-hao | 2008 | Journal of Hydrodynamics2008，20，6： | 3 |
| 6 | COVID-19-associated monocytic encephalitis(CAME):histological and proteomic evidence from autopsy显示文摘Severe neurological symptoms are associated with Coronavirus disease 2019(COVID-19).However,the morphologic features,pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic infection.In this study,neuropathological damages and infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining,ultrastructural examination under electron microscopy,and an image threshold method,in postmortem brains from nine critically ill COVID-19 patients and nine age-matched cadavers of healthy individuals.Differentially expressed proteins were identified by quantitative proteomic assays.Histopathological findings included neurophagocytosis,microglia nodules,satellite phenomena,extensive edema,focal hemorrhage,and infarction,as well as infiltrating mononuclear cells.Immunostaining of COVID-19 brains revealed extensive activation of both microglia and astrocytes,severe damage of the blood-brain barrier(BBB)and various degrees of perivascular infiltration by predominantly CD14+/CD16+/CD141+/CCR7+/CD11c+monocytes and occasionally CD4+/CD8+T lymphocytes.Quantitative proteomic assays combined with bioinformatics analysis identified upregulated proteins predominantly involved in immune responses,autophagy and cellular metabolism in COVID-19 patient brains compared with control brains.Proteins involved in brain development,neuroprotection,and extracellular matrix proteins of the basement membrane were downregulated,potentially caused by the activation of transforming growth factorβreceptor and vascular endothelial growth factor signaling pathways.Thus,our results define histopathological and molecular profiles of COVID-19-associated monocytic encephalitis(CAME)and suggest potential therapeutic targets. | Pei-Pei Zhang Zhi-Cheng He Xiao-Hong Yao Rui Tang Jie Ma Tao Luo Chuhong Zhu Tian-Ran Li Xindong Liu Dingyu Zhang Shuyang Zhang Yi-Fang Ping Ling Leng Xiu-Wu Bian | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 3 |
| 7 | Synthesis of N-methylene phosphonic chitosan(NMPCS)and its potential as gene carder显示文摘N-Methylene phosphoulc chitosan (NMPCS),an amphiphilic macromolecule with powerful chelating ability of Ca^(2+) ions,was synthesized and characterized.The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,^(13)C NMR,X-ray,agarose gel electrophorcsis retardation assay,atomic force microscopy (AFM) and circular dichroism (CD).The results suggest that at charge ratio 2:1 or above,DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed.Taking HeLa as host cell,luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan. | Dun Wan Zhu Jin Gen Bo Hai Ling Zhang Wen Guang Liu Xi Gang Leng Cun Xian Song Yu Ji Yin Li Ping Song Lan Xia Liu Lin Mei Xiu Lan Li Yang Zhang Kang De Yao | 2007 | Chinese Chemical Letters2007，18，11： | 3 |
| 8 | Heterogeneous Catalytic Ozonization of Sulfosalicylic Acid显示文摘This paper describes the potential of heterogeneous catalytic ozonization of sulfo-salicylic acid (SSal). It was found that catalytic ozonization in the presence of Mn-Zr-O (a modified manganese dioxide supported on silica gel) had significantly enhanced the removal rate (72%) of total organic carbon (TOC) compared with that of ozonization alone (19%). The efficient removal rate of TOC was probably due to increasing the adsorption ability of catalyst and accelerating decomposition of ozone to produce more powerful oxidants than ozone. . | Shao Ping TONG*, Wen Hua LENG, Jiang Qing ZHANG, Chu Nan CAO Department of Chemistry, Zhejiang University, Hangzhou 310027 | 2002 | Chinese Chemical Letters2002，13，6： | 2 |
| 9 | Heteropolyacids Anchored on Amino-Functionalized MCM-41 via Condensation as Reusable Catalysts for Esterification 显示文摘 | Wu Shanshan Liu Ping Leng Yan | 2009 | Catal Lett2009，132，34： | 1 |
| 10 | Cloning and functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits显示文摘 | Zhang Mei Leng Ping Zhang Guanglian Li Xiangxin | | 0，，： | 1 |
| 11 | 显示文摘 | Chen Junying Leng Yongxiang Yang Ping | 2002 | Biomaterials2002，23，： | 1 |
| 12 | The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit显示文摘 | Zhang Mei Yuan Bing Leng Ping | | 0，，06： | 1 |
| 13 | The principle of rapid detects annealed aluminum plate texture by X-ray 显示文摘 | MAO Wei-min CHEN Leng YANG Ping | 2004 | Chinese Science Bulletin2004，49，20： | 1 |
| 14 | Cloning and functional analysis of 9- cis -epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits显示文摘 | Mei Zhang Ping Leng Guanglian Zhang Xiangxin Li | 2009 | Journal of Plant Physiology2009，，12： | 1 |
| 15 | Bloodcompatibility improvement of titanium oxide film modified by phosphorus ion implantation 显示文摘 | YANG Ping LENG Yongxiang ZHAO Ansha | 2006 | Nuclear Instruments and Methods Physics Research B2006，24，： | 1 |
| 16 | Processing and mechanical properties of HA/UHMWPE nanocomposites 显示文摘 | Liming Fang Yang Leng Ping Gao | 2006 | Biomaterials2006，27，20： | 1 |
| 17 | On anycast routing with bandwidth constraint显示文摘 | Chor Ping Low Choor Leng Tan | 2003 | Intl Journal on Computer Communications2003，26，： | 1 |
| 18 | Processing of hydroxyapatite reinforced ultrahigh molecular weight polyethylene for biomedical applications 显示文摘 | Liming Fang Yang Leng Ping Gao | 2005 | Biomaterials2005，26，： | 1 |
| 19 | Biomarkers measured by cytokinesis-block micronucleus cytome assay for evaluating genetic damages induced by polycyclic aromatic hydrocarbons显示文摘 | Huawei Duan Shuguang Leng Zufei Pan Yufei Dai Yong Niu Chuanfeng Huang Ping Bin Yadong Wang Qingjun Liu Wen Chen Yuxin Zheng | 2009 | MutRes-Genetic Toxicology and Environmental Mutagenesis2009，，1： | 1 |
| 20 | Therapeutic effects of EGF-modified curcumin/ chitosan nano-spray on wound healing显示文摘Dermal injury,including trauma,surgical incisions,and burns,remain the most prevalent socio-economical health care issue in the clinic.Nanomedicine represents a reliable administration strategy that can promote the healing of skin lesions,but the lack of effective drug delivery methods can limit its effectiveness.In this study,we developed a novel nano-drug delivery system to treat skin defects through spraying.We prepared curcumin-loaded chitosan nanoparticles modified with epidermal growth factor(EGF)to develop an aqueous EGF-modified spray(EGF@CCN)for the treatment of dermal wounds.In vitro assays showed that the EGF@CCN displayed low cytotoxicity,and that curcumin was continuously and slowly released from the EGF@CCN.In vivo efficacy on wound healing was then evaluated using full-thickness dermal defect models in Wistar rats,showing that the EGF@CCN had significant advantages in promoting wound healing.On day 12 post-operation,skin defects in the rats of the EGF@CCN group were almost completely restored.These effects were related to the activity of curcumin and EGF on skin healing,and the high compatibility of the nano formulation.We therefore conclude that the prepared nano-scaled EGF@CCN spray represents a promising strategy for the treatment of dermal wounds. | Yue Li QingQing Leng XianLun Pang Huan Shi YanLin Liu SuSu Xiao Ling Zhao Ping Zhou ShaoZhi Fu | 2021 | Regenerative Biomaterials2021，8，2： | 1 |
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