| 1 | Efficacies of β-L-D4A against Hepatitis B virus in 2.2.15 cells显示文摘AIM:To investigate the antiviral effect of beta-L-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine (β-L-D4A) on 2.2.15 cells transfected with the hepatitis B virus (HBV) genome. METHODS:Lamivudine (3TC) as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen (HBsAg) in the culture supernatants were detected to determine the inhibitory effect of β-L-D4A. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect the survival ratio of 2.2.15 cells. RESULTS:β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication;this effect was apparent when the concentration was above 1 mol/L. When β-L-D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index (TI) of β-L-D4A was above 2.1. CONCLUSION:β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity. | Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li, Institute of Liver Diseases, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China Author contributions:Gao LL and Wang XY contributed equally to this work Gao LL, Wang XY, Lin JS, Zhang YH, Li Y designed the research Gao LL and Wang XY performed the research Gao LL wrote the paper | 2008 | World Journal of Gastroenterology2008，14，8： | 7 |
| 2 | 血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性显示文摘【据Joural of Viral Hepatitis 2020年8月报道】题:血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性(作者Chen YP等)研究表明,FIB-4及APRI等常规指数诊断慢性乙型肝炎肝硬化、显著肝纤维化在不同研究中存在差异。近期,研究者们也先后提出了多项新的常规指数,包括红细胞体积分布宽度-血小板比值(RPR)、谷氨酰转肽酶-血小板比值(GPR)及简易肝纤维化指数(eLIFT)。为评估ALT水平对这些常规指数诊断表现的影响,1005例慢性乙型肝炎患者被纳入回顾性分析,这些患者均已接受肝活检及常规血液检查。在该研究中,似然比10、0.1的对应值分别被确定为确认诊断、排除诊断界值。 | 林小钰 陈永鹏 CHEN YP HUANG LW LIN XY | 2020 | 临床肝胆病杂志2020，36，9： | 7 |
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