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| 1 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 2 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 3 | Urban Vegetation Landscape Fragmentation and the Change of Ecological Values in Shenzhen显示文摘Due to the increasing man-made activities,the vegetation landscape in the rapidly urbanized areas has changed dramatically.Based on the theory of landscape ecology and the evaluation method of ecosystem service function,this study took the vegetation of Shenzhen City as the research object,and used TM image as the main data source in 1985,1995,2005 and from Vol.4 of 2015 with the support of remote sensing and GIS technology to analyze the variation of vegetation landscape fragmentation and its ecological change.The results showed that the vegetation landscape fragmentation in Shenzhen City was significant in the past 30 years,and the vegetation landscape was the main characteristic in the fluctuation trend.The change trajectory of the vegetation landscape was the main focus,and the woodland land area was the largest land for construction.The fragmentation of vegetation landscape reduced the overall value of ecosystem services and hindered the function of life support systems. | LIAO Fangjun WANG Xiaoming | 2017 | Journal of Landscape Research2017，9，5： | 2 |
| 4 | Recruited CD68^(+)CD206^(+)macrophages orchestrate graft immune tolerance to prompt xenogeneic-dentin matrix-based tooth root regeneration显示文摘Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation,fibrosis,and remodeling process.Adaptive macrophage subsets have been identified to modulate inflammation and orchestrate the repair of neighboring parenchymal tissues.This study fabricated PPARγ-primed CD68+CD206+M2 phenotype(M2γ),and firstly verified their anti-inflammatory and tissue-regenerating roles in xenogeneic bioengineered organ regeneration.Our results showed that Th1-type CD3^(+)CD8^(+)T cell response to xenogeneic-dentin matrix-based bioengineered root complex(xeno-complex)was significantly inhibited by M2γmacrophage in vitro.PPARγactivation also timely recruited CD68^(+)CD206^(+)tissue macrophage polarization to xeno-complex in vivo.These subsets alleviated proinflammatory cytokines(TNF-α,IFN-γ)at the inflammation site and decreased CD3^(+)CD8^(+)T lymphocytes in the periphery system.When translated to an orthotopic nonhuman primate model,PPARγ-primed M2 macrophages immunosuppressed IL-1β,IL-6,TNF-α,MMPs to enable xeno-complex to effectively escape immune-mediated rejection and initiate graft-host synergistic integrity.These collective activities promoted the differentiation of odontoblast-like and periodontal-like cells to guide pulp-dentin and cementum-PDLs-bone regeneration and rescued partially injured odontogenesis such as DSPP and periostin expression.Finally,the regenerated root showed structure-biomechanical and functional equivalency to the native tooth.The timely conversion of M1-to-M2 macrophage mainly orchestrated odontogenesis,fibrogenesis,and osteogenesis,which represents a potential modulator for intact parenchymal-stromal tissue regeneration of targeted organs. | Hui Li Jingjing Sun Hefeng Yang Xue Han Xiangyou Luo LiJun Liao Bo Yang Tian Zhu Fangjun Huo Weihua Guo Weidong Tian | 2021 | Bioactive Materials2021，6，4： | 2 |
| 5 | Overview to the Hard X-ray Modulation Telescope (Insight-HXMT) Satellite显示文摘As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results. | Shuang-Nan Zhang TiPei Li FangJun Lu LiMing Song YuPeng X u CongZhan Liu Yong Chen XueLei Cao QingCui Bu Zhi Chang Gang Chen Li Chen TianXiang Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong Yuan Yuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan Can Gungor ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge Ou JinLu Qu Na Sai RenCheng Shang GuoHong Shen Liang Sun Ying Tan Lian Tao YouLi Tuo Chen Wang ChunQin Wang GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BaiYang Wu BoBing Wu Mei Wu GuangCheng Xiao ShaoLin Xiong LinLi Yan JiaWei Yang Sheng Yang YanJi Yang QiBin Yi Bin Yuan AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang ShenYi Zhangs Shu Zhang Tong Zhang WanChang Zhang Wei Zhang WenZhao Zhang Yi Zhang YiFei Zhang YongJie Zhang Yue Zhang Zhao Zhang Zhi Zhang ZiLiang Zhang HaiSheng Zhao XiaoFan Zhao ShiJie Zheng JianFeng Zhou YuXuan Zhu Yue Zhu RenLin Zhuang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 2 |
| 6 | Forest Landscape Change and Driving Forces in Nanling National Nature Reserve,Guangdong显示文摘Landscape change and its driving forces are always one of the major issues in landscape ecology. In this paper,we investigated the landscape pattern of Nanling National Nature Reserve using TM data and forest stand map. The paper aims at exploring how and why the landscape changed form 1988 to 2009. The results indicated that: the major landscape types are evergreen broad-leaved forest and coniferous forests,the area ratio of both total more than 80%; area ratio of other landscape types is less than 10%,including mainly mixed coniferous and broad-leaved forest,deciduous forest,shrub,other woodland,cultivated land,other land,construction land and water. During the 20 years,evergreen broad-leaved forest landscape has become a large-scale substrate,coniferous forests are interspersed,a small area of mixed coniferous and broad-leaved mixed forest patches expands; forest area in the study area changes slightly,the number of blocks increases,the degree of landscape fragmentation increases; forest landscapes change,and are less affected by human activities,which is closely related to the establishment,management and protection of protected areas. | LIAO Fangjun CHEN Zhiming | 2013 | Journal of Landscape Research2013，5，Z3： | 1 |
| 7 | RS-based Evaluation of Forest Cover Changes in Nanling National Nature Reserve显示文摘3S technology was applied to analyze spatio-temporal changes of landscape pattern in Nanling National Nature Reserve and the driving forces. The results showed that the study area was dominated by forest landscape from 1988 to 2009, accounting for 95% of the total area, among all forest landscapes, evergreen broadleaved forest accounted for the largest ratio(>50%). In terms of landscape fragmentation, landscape density index of the study area increased, landscape fragmentation was aggravated; patch density of core zone declined slightly, mean patch area of all landscape types increased, landscape fragmentation decreased slightly; buffer zone and experimental zone witnessed the aggravation of fragmentation. In terms of landscape diversity, landscape pattern of the study area became increasingly complex, diversity index increased, landscape heterogenization was enhanced; landscape diversity of the core zone increased slightly, that of the buffer zone increased greatly, but that of the experimental zone declined and landscape heterogenization reduced. Landscapes in the whole area grew more diversified, while landscape pattern of the core zone was simplified, that of the buffer zone witnessed slight changes, and that of the experimental zone kept consistent with that of the study area. In terms of driving forces of landscape pattern change, natural factors have contributed to the landscape changes in the study area, but human factors such as forest management, forest tourism, local residents an economic factors played a dominant role. With the increasing external interventions to the landscape pattern change, the contradiction between landscape eco-environment protection and resource development and utilization in Nanling National Nature Reserve will be increasingly sharpened. | LIU Zongjun LIAO Fangjun | 2015 | Journal of Landscape Research2015，7，5： | 0 |
| 8 | Growth,leaf anatomy,and photosynthesis of cotton(Gossypium hirsutum L.)seedlings in response to four light-emitting diodes and high pressure sodium lamp显示文摘Background Light is a critical factor in plant growth and development,particularly in controlled environments.Light-emitting diodes(LEDs)have become a reliable alternative to conventional high pressure sodium(HSP)lamps because they are more efficient and versatile in light sources.In contrast to well-known specialized LED light spectra for vegetables,the appropriate LED lights for crops such as cotton remain unknown.Results In this growth chamber study,we selected and compared four LED lights with varying percentages(26.44%–68.68%)of red light(R,600–700 nm),combined with other lights,for their effects on growth,leaf anatomy,and photosynthesis of cotton seedlings,using HSP lamp as a control.The total photosynthetic photon flux density(PPFD)was(215±2)μmol·m-2·s-1 for all LEDs and HSP lamp.The results showed significant differences in all tested parameters among lights,and the percentage of far red(FR,701–780 nm)within the range of 3.03%–11.86%was positively correlated with plant growth(characterized by leaf number and area,plant height,stem diameter,and total biomass),palisade layer thickness,photosynthesis rate(Pn),and stomatal conductance(Gs).The ratio of R/FR(4.445–11.497)negatively influenced the growth of cotton seedlings,and blue light(B)suppressed stem elongation but increased palisade cell length,chlorophyll content,and Pn.Conclusion The LED 2 was superior to other LED lights and HSP lamp.It had the highest ratio of FR within the total PPFD(11.86%)and the lowest ratio of R/FR(4.445).LED 2 may therefore be used to replace HPS lamp under controlled environments for the study of cotton at the seedling stage. | ZHANG Yichi LIAO Baopeng LI Fangjun ENEJI AEgrinya DU Mingwei TIAN Xiaoli | 2024 | Journal of Cotton Research2024，7，1： | 0 |
| 9 | Valuation on Forest Ecosystem Services of Nanling National Nature Reserve显示文摘The forest ecological system fulfills many important ecological functions. Researches on the analysis and valuation on forest ecosystem services are at the cutting edge of current ecology and ecological economics. Based on the calculation method of service value of forest ecosystem, 7 forest ecosystem services of Nanling National Nature Reserve were valued, totaling 703.48 million yuan/year. In terms of the contribution size of the service value of forest ecosystem, carbon fixation and oxygen release came as the greatest one, followed by conservation of soil, purification of the air, biodiversity conservation, conservation of water, accumulation of nutrients, and forest recreation. With its value of 208.46 million yuan, carbon fixation and oxygen release ranked first, accounting for 29.63% of the total value; conservation of soil of 24.67% service value took the second place; purification of the air came in third in the value rankings, representing 22.55% of the total value; the fourth one was biodiversity conservation, whose service value came up to 14.51%. Those 4 services made up 91.36% of the total value while other 3 services ranging from conservation of water, accumulation of nutrients to forest recreation were merely a small proportion. This is because the Nanling National Nature Reserve is essentially a large oxygen bar. The above-mentioned ecosystem valuation method contributes to the understanding of the overall value of the forest ecosystem as well as the implementation and advancement in the regional sustainable development strategy. | CHENG Zhiming LIAO Fangjun XIE Yong LIU Zongjun | 2017 | Journal of Landscape Research2017，9，3： | 0 |
| 10 | Study on Production Process of Roselle Rice Wine显示文摘[Objectives]This study was conducted to more systematically explore the traditional brewing process of roselle rice wine. [Methods] With local high-quality white glutinous rice and sun-dried roselle as raw materials for brewing rice wine, on the premise of extensive research on traditional rice wine technology in China, the addition amount of roselle extract, the addition amount of distiller’s yeast, the variation range of after-fermentation temperature and the starting time of after-fermentation were investigated. [Results] The best traditional processing and brewing parameters were the ratio of glutinous rice and roselle extract(mass to volume ratio) at about 1∶1.55, the addition amount of distiller’s yeast of 0.55%, and the fermentation temperature at 30 ℃, and the starting time at 72 h. The sensory quality of the high-quality roselle rice wine that was produced under the best brewing conditions was about(87.7±2.45) points. [Conclusions] A number of hygiene inspection technical indexes in the product all met the current relevant national safety standards in China, providing another technical research direction for the development of a variety of new green healthy beverage projects. | Fangjun LIAO Sumei HUANG | 2022 | Agricultural Biotechnology2022，11，5： | 0 |
| 11 | Functional Division Analysis and Optimization Design of Nanling Nature Reserve显示文摘Nanling National Nature Reserve is established on the basis of 5 provincial nature reserves, and the original functional zoning is maintained. This paper explored problems of the reserve in division range and functional zoning, and gavea pertinent adjustment scheme. The adjustment results are(1) cutting out the scattered areas in neighborhood, and fi lling in the internal split parts;(2) establishing corridors between the reserve and neighboring nature reserves;(3) redividing the functional zones. | XIE Yong LIAO Fangjun | 2016 | Journal of Landscape Research2016，8，3： | 0 |
| 12 | Using Urban Landscape Trajectories to Assess Fragmentation in Mountainous Coastal Areas: A Case Study of Shenzhen, China显示文摘Rapid urbanization in mountainous coastal areas causes dramatic changes in land use and land cover(LUCC)for their geographic location.To understand changes in landscape trajectories in such regions,we examined temporal and spatial changes in urban landscapes in Shenzhen,China,a city that has undergone rapid urbanization in recent decades,and investigated the drivers of landscape pattern evolution at different scales from LUCC data from 1985,1995,2005,and 2015.The results show that the landscape of Shenzhen has changed dramatically over the 30 years from 1985 to 2015.The proportion of forest area decreased from 50.81%to 37.81%,while the proportion of construction land increased from 17.83%to 48.28%.The changes in the landscape patterns in Shenzhen were driven mainly by human activity.This study is one of the first to assess landscape dynamics of the rapid urbanization from the angle of mountainous coastal area,which plays an important role in the land use and city regulation of the global coastal mountains. | Fangjun LIAO Xiaoming WANG | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
| 13 | Study on Tissue Culture of Japanese Honeysuckle ( Lonicera japonica )显示文摘[Objectives]This study was conducted to increase the reproduction coefficient of Japanese honeysuckle(Lonicera japonica)to keep the character of optimal benign.[Methods]The young leaves of medicinal Japanese honeysuckle were selected as explants,and MS was used as the basic culture medium.Suitable culture concentrations and conditions were screened through different concentration gradients of growth regulators and cytokinin.[Results]As the concentration of 6-BA in the culture medium increased,the browning rate increased,and the browning phenomenon occurred earlier.On the contrary,a lower concentration of 6-BA was suitable for the differentiation and growth of young leaves,and the browning response was slow.However,if the cultivation time was too long and the materials were not transferred in a timely manner,browning would also occur.The optimal combination of levels was obtained through a 3×3 orthogonal experiment(three parallel groups for each of 6-BA and NAA).The culture conditions included a constant temperature of 26℃and light intensity of 1200 lx.The optimal medium for inducing callus proliferation was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;and the optimal medium for inducing bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.[Conclusions]This study provides a theoretical basis for accelerating the development of the honeysuckle industry. | Fangjun LIAO Xiaoru CHEN | 2023 | Agricultural Biotechnology2023，12，5： | 0 |
| 14 | The Development Situation and Countermeasures on Phalaenopsis aphrodite Industry in Guangdong显示文摘The Phalaenopsis aphrodite industry has the characteristics of high technology content,industrialization,modern management,organic combination of industry and strong radiation effect. On the basis of investigation,we expounded the current development situation of the P. aphrodite industry in Guangdong,analyzed the aspects of production elements and demand conditions based on the ' diamond theory',and put forward suggestion and countermeasures for the development of the P. aphrodite industry. | Junhong ZHONG Fangjun LIAO Xiulian LIN | 2020 | Agricultural Biotechnology2020，9，3： | 0 |
| 15 | In-orbit performance of ME onboard Insight-HXMT in the first 5 years显示文摘Introduction The medium-energy X-ray telescope(ME)is a collimated X-ray telescope onboard the Insight hard X-ray modulation telescope(Insight-HXMT)satellite.It has 1728 Si-PIN pixels readout using 54 low noise application-specific integrated circuits(ASICs).ME covers the energy range of 5–30 keV and has a total detection area of 952cm2.The typical energy resolution of ME at the beginning of the mission is 3 keV at 17.8 keV(full width at half maximum,FWHM),and the time resolution is 255μs.In this study,we present the in-orbit performance of ME in its first 5 years of operation.Methods The performance of ME was monitored using onboard radioactive sources and astronomical X-ray objects.ME carries six 241Am radioactive sources for onboard calibration,which can continuously illuminate the calibration pixels.The long-term performance evolution of ME can be quantified using the properties of the accumulated spectra of the calibration pixels.In addition,observations of the Crab Nebula and the pulsar were used to check the long-term evolution of the detection efficiency as a function of energy.Conclusion After 5 years of operation,742cm2 of the Si-PIN pixelswere stillworking normally.The peak positions of 241Am emission lines gradually shifted to the high-energy region,implying a slow increase in ME gain of 1.43%.A comparison of the ME spectra of the Crab Nebula and the pulsar shows that the E–C relations and the redistribution matrix file are still acceptable for most data analysis works,and there is no detectable variation in the detection efficiency. | Ying Tan Xuelei Cao Weichun Jiang Xiaobo Li Bin Meng Wanchang Zhang Sheng Yang Tao Luo Yudong Gu Liang Sun Xiaojing Liu Yuanyuan Du Jiawei Yang Yanjun Xu Jinyuan Liao Yupeng Xu Fangjun Lu Liming Song Shuangnan Zhang | 2023 | Radiation Detection Technology and Methods2023，7，1： | 0 |
| 16 | Pollution and Control Measures in Plant Tissue Culture in Orchid Plant显示文摘Contamination is a phenomenon that often occurs in the operation of plant tissue culture,and it is also one of the three major problems in plant tissue culture.Compared with browning and vitrification,contamination is more likely to occur,which brings great harm to scientific research and production practice.Its appearance will greatly affect the normal growth and differentiation of tissue culture materials,and will reduce the yield of cultivated plants to a certain extent.Therefore,we cannot underestimate any step in the tissue culture operation.This study summarized the reasons for its occurrence and how to formulate prevention and control measures based on recent research combined with the actual situation. | Fangjun LIAO Xiaoling YE Sumei HUANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
| 17 | Tissue Culture and Rapid Propagation System of Roselle(Hibiscus sabdariffa L.)显示文摘[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle. | Zifan GUI Fangjun LIAO Xiaoling YE Sumei HUANG Liqing WANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
| 18 | Tissue Culture Application of Maranta arundinacea显示文摘The reproductive methods of ornamental arrowroot(Maranta arundinacea)mainly include plant division,cutting propagation,truncation and sprout promotion propagation,and tissue culture.Large-scale seedling production mainly relies on tissue culture.In the process of tissue culture,the culture conditions can be artificially controlled,and the plant materials used for tissue culture grow completely in artificially-provided culture media and microclimate environment,free from the adverse effects of catastrophic climate,seasonal and diurnal changes.Because the conditions are relatively uniform,tissue culture is very beneficial for plant growth and more convenient for stable long-term cultivation and production.In the process of tissue culture,the inoculation process is more important.How can we achieve standardized operation and reduce pollution during the inoculation process is worth exploring. | Fangjun LIAO Xiaoling YE Fanshu HUANG | 2023 | Agricultural Biotechnology2023，12，4： | 0 |
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