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| 1 | Structural insights into the TRIM family of ubiquitin E3 ligases显示文摘 | Yang Li Han Wu Wei Wu Wei Zhuo Weixiao Liu Yixiao Zhang Minzhang Cheng Ye-Guang Chen Ning Gao Hongtao Yu Linfang Wang Wei Li Maojun Yang | 2014 | Cell Research2014，24，6： | 12 |
| 2 | The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis显示文摘 | Fang Wang Wei Song Hongmei Zhao Yanni Ma Yuxia Li Di Zhai Jingnan Pi Yanmin Si Jiayue Xu Lei Dong Rui Su Mengmeng Zhang Yong Zhu Xiaoxia Ren Fei Miao Wenjie Liu Feng Li Junwu Zhang Aibin He Ge Shan Jingyi Hui Linfang Wang Jia Yu | 2017 | Cell Research2017，27，3： | 7 |
| 3 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 4 | Parthenolide inhibits LPS-induced inflammatory cytokines through the toll-like receptor 4 signal pathway in THP-1 cells显示文摘Parthenolide (PTL ) 显示出反煽动性的有势力和反癌症活动。在现在的学习, PTL 的活动的分子的机制在导致的 lipopolysaccharide (LPS ) 被探索人的白血病 monocytic THP-1 房间和人的主要单核白血球。3-(4,5-dimethylthiazol-2-yl )-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 盐(山) 试金被用来在 THP-1 房间生存能力上分析 PTL 的效果。连接酶的 immunosorbent 试金被用来在导致 LPS 的煽动性的 cytokine 分泌物上决定 PTL 的效果。流动 cytometry 和量的即时聚合酶链反应被用来在导致 LPS 的像使用费的受体上估计 PTL 的效果 4 (TLR4 ) 表示。表明分子的 Phosphorylation 层次被西方的污点分析决定。结果显示出那 PTL < 12.5 M 显著地没影响 THP-1 房间生存能力。LPS 治疗导致了 interleukin (IL ) 的一条显著起来规定 -6, IL-1, IL-8, IL-12p40,肿瘤坏死因素 -- , IL-18,并且没有在里面 THP-1 房间。然而, PTL 以一种剂量依赖者方式禁止了这些 cytokines 的表示,与 1.0912.620 M 的 IC 50 价值。PTL 与由流动 cytometry 分析决定了的 1.373 M 的 IC 50 值堵住了 TLR4 表示,并且这堵住的效果在蛋白质和 mRNA 层次被验证。另外的分子的表情的细胞外的调整信号的 kinase 1/2 ,6月N终端 kinase , p38 ,原子因素 B ( NF-B ) p65 ,和 IB 和起来规定的 phosphorylation 层次的起来规定(可诱导的氮的氧化物 synthase , TLR4 ,并且 TNF 联系受体的因素 6 )由 LPS 导致了被 PTL 以一种剂量依赖者方式废除。PTL 的反煽动性的机制通过表明小径的调停 TLR4 的激活 mitogen 的蛋白质 kinase 和 NF-B 部分操作。因此, TLR4 可以是为反发炎治疗的一个新目标。 | Shuangshuang Li Xiangli Gao Xiaoxin Wu Zhigang Wu Linfang Cheng Lifen Zhu Dan Shen Xiangmin Tong | 2015 | Acta Biochimica et Biophysica Sinica2015，47，5： | 5 |
| 5 | Atorvastatin protects cardiomyocytes from oxidative stress by inhibiting LOX-1 expression and cardiomyocyte apoptosis显示文摘冠的动脉疾病(CAD ) 是世界范围的一个主要健康问题。CAD 的最严重的形式是急性冠的症候群(交流) 。最近的研究在交流表明了 atorvastatin 的有益的角色;然而,位于这效果下面的机制充分没被澄清。成长证据显示氧化低密度的脂蛋白 receptor-1 (LOX-1 ) 玩的象 lectin 一样的那激活在氧化导致压力的 cardiomyocyte apoptosis 的一个重要角色在交流期间。在这研究,我们检验了 atorvastatin 是否禁止 H 2 O 2-induced LOX-1 表示和 H9c2 cardiomyocyte apoptosis,并且调查了内在的发信号小径。有 H 2 O 2 导致了 LOX-1 mRNA 和蛋白质的提高的表示,以及增加了 caspase-3 和 -9 蛋白质表示和房间 apoptosis。H 2 O 2-induced LOX-1 表示, caspase 蛋白质表示,和 cardiomyocyte apoptosis 被预告的处理与 atorvastatin 稀释。Atorvastatin 激活 H 2 以一种集中依赖者方式的 Akt 的 O 2-inhibited phosphorylation。Akt 禁止者, LY294002,在导致 Akt phosphorylation 上并且在压制 H 2 O 2-mediated caspase 起来规定和房间 apoptosis。这些调查结果显示 atorvastatin 经由 LOX-1 的抑制保护 cardiomyocyte 免受氧化应力的伤害表示和 apoptosis,和 H 2 Akt 的 O 2-inhibited phosphorylation 可以在 atorvastatin 的保护的功能起一个重要作用。 | Lei Zhang Linfang Cheng Qiqi Wang Dongchen Zhou Zhigang Wu Ling Shen Li Zhang Jianhua Zhu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，3： | 4 |
| 6 | Controlled growth and photoluminescence of highly oriented arrays of ZnO nanocones with different diameters显示文摘Large-scale oriented ZnO nanocone arrays were directly grown on zinc substrate through a hydro-thermal reaction of Zn foil with aqueous butylamine solution(3 mol/L) at 100-180 ℃ for 12 h.The syn-thesized products were characterized with X-ray diffraction,Raman spectrum,scanning electron mi-croscopy and transmission electron microscopy.The results showed that the ZnO nanocones were single crystalline with the wurtzite structure and grown along the [0001] direction.The diameter of nanocones is decreased with increasing the reaction temperature.A possible growth mechanism was also proposed to account for the formation of the ZnO nanocone arrays.The photoluminescence spectra of the ZnO nanocone arrays were studied at room temperature,two UV emission bands at 377 and 396 nm assigned to free exciton emission and exciton-exciton collision,respectively,and phonon replicas associated with 2-E2 phonon were observed in the PL spectra. | MA JunHu YANG HeQing SONG YuZhe LI Li XIE XiaoLi LIU RuiNi WANG LinFang | 2009 | Science China(Technological Sciences)2009，52，5： | 4 |
| 7 | Clear mortality gap caused by graft macrosteatosis in Chinese patients after cadaveric liver transplantation显示文摘Background:Liver transplantation(LT)is one of the most effective surgical treatment for patients with end-stage liver disease.Steatosis is a contributor for inferior graft quality.But its impact and safety on transplantation was less assessed in Chinese patients.Methods:Graft steatosis and related information involved in recipients,donors and surgical procedures were retrospectively collected from 239 patients.Results:Donor macrosteatosis(MaS)caused about 2.14 and 2.80 folds of increment on patient and graft mortality.Dose-response analysis revealed prominent risk of grafts on overall patient/organ mortality when MaS content exceeded 10%(P<0.05).Noteworthy,deaths were only observed in MaS group when concurrent with extremely higher post-transplant alanine aminotransferase(ALT,64%).However,microsteatosis(MiS)grafts didn’t affect outcomes after LT.In a cohort of Chinese patients,MaS had comprehensive effects on post-transplant outcomes with relatively lower safety threshold at 10%.Mortality gap caused by MaS grafts was observed in patients with severer ischemia reperfusion injury.Conclusions:Our study revealled the graft MaS affected the post-transplant outcomes in lower risk cutoff in Chinese patients.Further study is worthy to validate these results and investigate inner mechanism under the phenomenon. | Zhengtao Liu Wenchao Wang Li Zhuang Jingfeng Liu Shuping Que Dan Zhu Linfang Dong Jian Yu Lin Zhou Shusen Zheng | 2020 | Hepatobiliary Surgery and Nutrition2020，9，6： | 2 |
| 8 | Silicon substrate-integrated hollow waveguide for miniaturized optical gas sensing显示文摘Gas sensors have a wide variety of applications.Among various existing gas sensing technologies,optical gas sensors have outstanding advantages.The development of the Internet of Things and consumer electronics has put stringent requirements on miniaturized gas sensing technology.Here,we demonstrate a chip-scale silicon substrate-integrated hollow waveguide(Si-iHWG) to serve as an optical channel and gas cell in an optical gas sensor.It is fabricated through silicon wafer etching and wafer bonding.The Si-i HWG chip is further assembled with an off-chip light source and detector to build a fully functional compact nondispersive infrared(NDIR) CO_(2) sensor.The chip size is 10 mm × 9 mm,and the dimension of the sensor excluding the microcontroller board is 50 mm × 25 mm × 16 mm.This chip solution with compactness,versatility,robustness,and low cost provides a cost-effective platform for miniaturized optical sensing applications ranging from air quality monitoring to con-sumer electronics. | SHAONAN ZHENG HONG CAI LINFANG XU NANXI LI ZHONGHUA GU YAO ZHANG WEIGUO CHEN YANYAN ZHOU QINGXIN ZHANG LENNON YAO TING LEE | 2022 | Photonics Research2022，10，1： | 2 |
| 9 | Risk stratification in symptomatic intracranial atherosclerotic disease with conventional vascular risk factors and cerebral haemodynamics显示文摘Background and purpose Symptomatic intracranial atherosclerotic stenosis(sICAS)is associated with a considerable risk of recurrent stroke despite contemporarily optimal medical treatment.Severity of luminal stenosis in sICAS and its haemodynamic significance quantified with computational fluid dynamics(CFD)models were associated with the risk of stroke recurrence.We aimed to develop and compare stroke risk prediction nomograms in sICAS,based on vascular risk factors and these metrics.Methods Patients with 50%-99%sICAS confirmed in CT angiography(CTA)were enrolled.Conventional vascular risk factors were collected.Severity of luminal stenosis in sICAS was dichotomised as moderate(50%-69%)and severe(70%-99%).Translesional pressure ratio(PR)and wall shear stress ratio(WSSR)were quantified via CTA-based CFD modelling;the haemodynamic status of sICAS was classified as normal(normal PR&WSSR),intermediate(otherwise)and abnormal(abnormal PR&WSSR).All patients received guideline-recommended medical treatment.We developed and compared performance of nomograms composed of these variables and independent predictors identified in multivariate logistic regression,in predicting the primary outcome,recurrent ischaemic stroke in the same territory(SIT)within 1 year.Results Among 245 sICAS patients,20(8.2%)had SIT.The D2H2A nomogram,incorporating diabetes,dyslipidaemia,haemodynamic status of sICAS,hypertension and age≥50 years,showed good calibration(P for Hosmer-Lemeshow test=0.560)and discrimination(C-statistic 0.73,95%CI 0.60 to 0.85).It also had better performance in risk reclassification and provided larger net benefits in decision curve analysis,compared with nomograms composed of conventional vascular risk factors only,and plus the severity of luminal stenosis in sICAS.Sensitivity analysis in patients with anterior-circulation sICAS showed similar results.Conclusions The D2H2A nomogram,incorporating conventional vascular risk factors and the haemodynamic significance of sICAS as assessed in CFD models,could be a useful tool to stratify sICAS patients for the risk of recurrent stroke under contemporarily optimal medical treatment. | Xuan Tian Hui Fang Linfang Lan Hing Lung Ip Jill Abrigo Haipeng Liu Lina Zheng Florence S Y Fan Sze Ho Ma Bonaventure Ip Bo Song Yuming Xu Jingwei Li Bing Zhang Yun Xu Yannie O Y Soo Vincent Mok Ka Sing Wong Thomas W Leung Xinyi Leng | 2023 | Stroke & Vascular Neurology2023，8，1： | 2 |
| 10 | The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation显示文摘Acetaminophen(APAP)overdose is a major cause of liver injury.Neural precursor cell expressed developmentally downregulated 4—1(NEDD4-1)is an E3 ubiquitin ligase that has been implicated in the pathogenesis of numerous liver diseases;however,its role in APAP-induced liver injury(AILI)is unclear.Thus,this study aimed to investigate the role of NEDD4-1 in the pathogenesis of AILI.We found that NEDD4-1 was dramatically downregulated in response to APAP treatment in mouse livers and isolated mouse hepatocytes.Hepatocyte-specific NEDD4-1 knockout exacerbated APAP-induced mitochondrial damage and the resultant hepatocyte necrosis and liver injury,while hepatocyte-specific NEDD4-1 overexpression mitigated these pathological events both in vivo and in vitro.Additionally,hepatocyte NEDD4-1 deficiency led to marked accumulation of voltage-dependent anion channel 1(VDAC1)and increased VDAC1 oligomerization.Furthermore,VDAC1 knockdown alleviated AILI and weakened the exacerbation of AILI caused by hepatocyte NEDD4-1 deficiency.Mechanistically,NEDD4-1 was found to interact with the PPTY motif of VDAC1 through its WW domain and regulate K48-linked ubiquitination and degradation of VDAC1.Our present study indicates that NEDD4-1 is a suppressor of AILI and functions by regulating the degradation of VDAC1. | Yiwei Zhu Lin Lei Xinghui Wang Linfang Chen Wei Li Jinxia Li Chenchen Zhao Xiliang Du Yuxiang Song Wenwen Gao Guowen Liu Xinwei Li | 2023 | Acta Pharmaceutica Sinica B2023，13，4： | 1 |
| 11 | The Probability Analysis for the Many-Versus-One Guerrilla War显示文摘This paper discusses a stochastic duel model between two forces. On one side is the guerrilla(or terrorists) which has only one weapon(or person), and on the other an organized force of some sort which has many weapons. The model is called the many-versus-one guerrilla war. The guerrilla side has a number of advantages such as choice of location and time of engagement, concealment by topography, observation of the intended target, and line of fire. The authors present these advantages in what they believe are a realistic scenario of a duel between the guerrilla force and the organized force. By the four suppositions coinciding with the practical duel background, the paper presents the formulas to calculate the satisfying probability, the weak satisfying probability, and the acceptable probability of the attack side. | LIU Liwei LI Jianjun QIAN Linfang | 2015 | Journal of Systems Science & Complexity2015，28，3： | 1 |
| 12 | Conversion of rat embryonic stem cells into neural precursors in chemical-defined medium显示文摘 | Xinrong Peng Haixia Gao Ying Wang Baotian Yang Tao Liu Yan Sun Huajun Jin Lihua Jiang Linfang Li Mengchao Wu Qijun Qian | 2013 | Biochemical and Biophysical Research Communications2013，，： | 1 |
| 13 | OPTIMUM DESIGN OF HIGH-SPEED RAILWAY LOCOMOTIVE TRACTION GEAR显示文摘OPTIMUMDESIGNOFHIGH-SPEEDRAILWAYLOCOMOTIVETRACTIONGEARLiLixing;WangXiuqi;HeWeidong;ZhaoWenzhong;QianYuanmei(DalianRailwayInst... | Li Lixing Wang Xiuqi He Weidong Zhao Wenzhong Qian Yuanmei (Dalian Railway Institute) Liu Youmei Gui Linfang Xiao Peng Pan Xiaoming Xu Zongxiang (Zhuzhou Electric Locomotive Works ) | 1996 | Chinese Journal of Mechanical Engineering1996，9，4： | 1 |
| 14 | Identification of quantitative trait loci for seed storability in rice ( Oryza sativa L.)显示文摘 | LINFANG LI QIUYUN LIN SHIJIA LIU XI LIU WENYAN WANG NGO THI HANG FENG LIU ZHIGANG ZHAO LING JIANG JIANMIN WAN | 2012 | Plant Breeding2012，，6： | 1 |
| 15 | Neuroprotective effect of shenfu injection after intra-arterial thrombolysis显示文摘BACKGROUND: Within 3 hours after ischemic stroke, thrombolysis can improve prognosis of patients; however, cerebral edema and cerebral hemorrhage induced by vascular recanalization at an early phase can influence on therapeutic effect of thrombolysis; therefore, thrombolysis is combined with neuroprotective agent for recently clinical treatment. OBJECTIVE: To evaluate the neuroprotective effect of shenfu injection on patients with ischemic stroke after arterial thrombolysis and compare with the controls. DESIGN: Case-controlled study. SETTING: Department of Medical Image, Second Hospital of Hebei Medical University. PARTICIPANTS: A total of 45 patients with acute stroke were selected from Emergency Department or Neurological Department of the Second Hospital of Hebei Medical University from August 2005 to May 2006. All patients were met the diagnosis criteria published by the Fourth National Stroke Conference and had middle cerebral arterial embolism with angiography. Attacked course was less than 6 hours. Patients did not have a history of stroke. Scores of the National Institutes of Health Stroke Scale (NIHSS) were not less than 4 points and patients were aged from 18 to 80 years. All subjects consented of the whole therapeutic process. METHODS: ① Intermittent perfusion was used to treat intra-arterial thrombolysis. Thirty patients with vascular recanalization or partially vascular recanalization (18 males and 12 females) were randomly divided into treatment group and control group with 15 in each group. There was no significant difference of baseline data between the two groups. After recanalization, patients in treatment group were given 20 mL shenfu injection, which consisted of ginseng saponin and aconitine (Ya’an Sanjiu Pharmaceutical Company, batch number: 041013, 10 mL/ampoule) and slowly injected into vein for longer than 5 minutes. And then, patients were intravenously dripped with 50 mL shenfu injection and 50 g/L 250 mL glucose solution once a day for 2 courses. Fourteen days were regarded as a course. Patients in control group were treated with the same volume of glucose solution (50 g/L), and other processes in this group were as the same as those in treatment group. ② NIHSS scores before thrombolysis and at 24 hours after thrombolysis: Patients were scored as the follows: level of consciousness, staring, sight, facial paralysis, upper limb movement, lower limb movement, incoordination, sense, language, dysarthria and neglect. Total scores ranged from 0 to 42, and the more the scores were, the more obvious the symptom was. ③ Patients were checked with head CT examination at 24 hours after thrombolysis. ④ Modified Rankin scores at 3 months after thrombolysis: Total scores ranged from 0 to 6. 0: symptomless; 6: death; 0-2: well; 3-6: poor. ⑤ Adverse events and side effects were checked after thrombolysis. MAIN OUTCOME MEASURES: ① Comparison of NIHSS scores between the two groups before and after thrombolysis; ② comparison of modified Rankin scores at 3 months after thrombolysis; ③ comparison of adverse events and side effects. RESULTS: Among 45 patients with ischemic stroke, 30 ones with vascular recanalization were involved in the final analysis. ① Comparison of NIHSS scores before and after thrombolysis: After thrombolysis, NIHSS scores were 5 in treatment group and 8 in control group, which were lower than those before thrombolysis (12, 13; U =2.054, 1.652; P < 0.05). At 24 hours after thrombolysis, NIHSS score was 5 in treatment group, which was lower than that in control group (8, U =2.157, P < 0.05). ② Comparison of modified Rankin scores at 3 months after thrombolysis: Rankin scores which were regarded as well accounted for 93% (14/15) in treatment group, which was higher than those in control group (53%, 8/15, P < 0.05). ③ Comparison of adverse events and side effects: Patients in treatment group did not have cerebral hemorrhage and whole-body hemorrhage with head CT examination; meanwhile, all patients were living at 24 hours after thrombolysis. Among 2 patients with cerebral hemorrhage, 1 case were dead in control group with head CT examination at 24 hours after thrombolysis. CONCLUSION: Intra-arterial thrombolysis combined with shenfu injection plays a neuroprotective role in ischemic stroke; additionally, it is safe for patients. | Huaijun Liu Xiuchuan Jia Jiping Yang Zengpin Liu Guoshi Wang Linfang Li | 2006 | Neural Regeneration Research2006，1，5： | 1 |
| 16 | Numerical Study of Parachute-Payload Land Site Distribution with Randomize Wind Gust Model显示文摘A parachute-payload model with randomize wind gust is developed to study the landing accuracy of the parachute decelerator system,which can be exactly described by the landing site distribution.The research focuses on the steady descent phase of the parachute descent process,so the parachute and the payload suspension formulation during the phase are mainly discussed.In addition,since the wind effects have a significant impact on the land site distribution of the passive decelerator system and it is difficult to obtain the exact wind profile in practice,major features of parachute-payload system are studied via the randomized wind gust formulation.As the randomized wind gust formulation is adopted,the wind effect can be considered without the exact wind gust profile and the parachute aerodynamic simulation can be fulfilled with uncertainties.Finally,the model is validated and discussed,and the parachute land site distributions with different wind randomize profiles are presented for comparison.The results show that when parachute is less stable,the land site tends to have a larger variance. | Tang Jianhua Qian Linfang Yin Qiang Jiang Li | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，2： | 1 |
| 17 | Evaluation of a semi-quantitative ELISA for IgA antibody against Epstein–Barr virus capsid antigen in the serological diagnosis of nasopharyngeal carcinoma显示文摘 | Hao Chen Peidong Chi Weida Wang Linfang Li Yaoling Luo Jihuan Fu Liuyan Zhang Wanli Liu | 2014 | International Journal of Infectious Diseases2014，，： | 1 |
| 18 | Graft co-polymerization of maleic acid and vinyl acetate onto poly(vinylidene fluoride) powder by pre-irradiation technique显示文摘Binary monomers of maleic acid and vinyl acetate are grafted onto poly(vinylidene fluoride) (PVDF) powder by pre-irradiation induced graft polymerization technique in emulsion solution. The co-grafting of binary monomers is successfully proved by FT-IR spectroscopy analysis. The influences of the absorbed dose, reaction temperature, reaction time, total concentration of monomers, and feed ratio of binary monomers on the degree of grafting are investigated. The thermal property of grafted PVDF powder was characterized by differential scanning calorimetry (DSC) and thermo-gravimetry analysis (TGA). | ZHANG Bowu WEI Rongmao YU Ming DENG Bo LI Linfang LI Jingye | 2012 | Nuclear Science and Techniques2012，23，2： | 1 |
| 19 | Neuroprotection of a cocktail therapy involving anti-intercellular adhesion molecule 1 antibody and insulin-like growth factor 1 in treatment of cerebral ischemia/reperfusion injury in cats Nuclear magnetic resonance examination and pathological validatio显示文摘BACKGROUND: The pathophysiological mechanisms of ischemia are extremely complicated. It is difficult to confirm and maintain the therapeutic effects if only one neuroprotective agent is used. It is hypothesized that a cocktail therapy involving a combined application of neuroprotective agents is feasible and offers excellent therapeutic potential. OBJECTIVE: To evaluate the neuroprotective effects of a cocktail therapy of insulin-like growth factor 1 (IGF1) combined with anti-intercellular adhesion molecule 1 (ICAM1) antibody in the treatment of cerebral ischemia/reperfusion injury using medical imaging, pathology, and functional neurological deficit scoring techniques. DESIGN, TIME AND SETTING: This randomized, controlled, neuroimaging analysis of function and pathological observation was performed at the Laboratory of Molecular Imaging, Second Hospital, Hebei Medical University between September 2006 and December 2007. MATERIALS: Transient middle cerebral artery occlusion (MCAO) was induced in 24 healthy adult cats. Anti-ICAM1 antibody and IGF1 were sourced from the Shanghai Kangcheng Biological Product Co., Ltd., China. Stereotaxic apparatus was purchased from the Center for Medical Apparatus and Instruments, Shandong Liaocheng People’s Hospital, China. The in situ apoptosis kit was provided by the Beijing Zhongshan Biotechnique Co., Ltd., China. METHODS: Twenty-four cat models of MCAO were randomly divided into four groups (n = 6): control, IGF1, anti-ICAM1 antibody and cocktail therapy. Following a 2-hour ischemia and subsequent lateral cerebral ventricular puncture, 100 μg IGF1(cerebral ventricular), 100 μg anti-ICAM1 antibody (i.v.), 50 μg IGF1(lateral cerebral ventricular) + 50 μg anti-ICAM1 antibody (i.v.), and 100 μg physiological saline (i.v.) were administered to the IGF1, anti-ICAM1 antibody, cocktail therapy and control groups, respectively. On the following day, the same administration was performed again. MAIN OUTCOME MEASURES: Pathological observation of the cerebral dura mater tissue surrounding the MCAO target site was performed by electron microscopy. At days 3 and 7 following MCAO induction, the volume of the cerebral infarction was measured by Philips functional neurological deficit scoring and nuclear magnetic resonance imaging. RESULTS: Pathological observation revealed that the control group exhibited a great number of swollen neurons, glial cells and vascular endothelial cells, and showed severely injured mitochondria with an absence of the double membrane structure. The same phenomena were partially alleviated in the IGF1 and anti-ICAM1 antibody groups, and the most obvious alleviation of injuries was in the cocktail therapy group. At day 6 following MCAO establishment, the cocktail therapy group exhibited the smallest cerebral infarction volumes among the four groups (F = 71.322, P < 0.01). At days 3 and 7 following MCAO induction, the F value of Philips functional neurological deficit scoring was 10.398 and 14.430, respectively (P < 0.01); however, the best neurological functional recovery was in the cocktail therapy group. CONCLUSION: A cocktail therapy of IGF1 combined with anti-ICAM1 antibody produces better neuroprotective effects than IGF1 or anti-ICAM1 alone, as judged by injury to mitochondria and swelling in and around neurons and glial cells. | Huaijun Liu Liqun Yan Yaping Hou Shuochun Wu Dan He Linfang Li Yankai Wu BoyuanHuang Fei Yang | 2008 | Neural Regeneration Research2008，3，12： | 0 |
| 20 | Undercooling evolution of pure Sn droplets in various atmospheres based on fast scanning calorimetry显示文摘Undercooling of Sn droplets in different atmospheres was studied by fast scanning calorimetry(FSC)at cooling rate of 1,000 K/s.It is found that the undercooling decreased with increasing partial pressure of oxygen.Randomly distributed SnO2islands were observed to form on the droplet surface,which likely has promoted the heterogeneous surface nucleation.As the partial pressure of oxygen changes,the nucleation rate and growth of SnO2led to different oxide islands,which resulted in various potential catalytic sites for the nucleation of the molten Sn droplet.The results showed that the nucleation process of the Sn droplets was sensitive to the solidification environment,and therefore the atmosphere should be taken into account in the study of the nucleation behavior of the single Sn droplets. | Bingge Zhao Linfang Li Qijie Zhai Yulai Gao | 2014 | Chinese Science Bulletin2014，59，20： | 0 |
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