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| 1 | Structural insights into the TRIM family of ubiquitin E3 ligases显示文摘 | Yang Li Han Wu Wei Wu Wei Zhuo Weixiao Liu Yixiao Zhang Minzhang Cheng Ye-Guang Chen Ning Gao Hongtao Yu Linfang Wang Wei Li Maojun Yang | 2014 | Cell Research2014，24，6： | 12 |
| 2 | Amazing structure of respirasome: unveiling the secrets of cell respiration显示文摘Respirasome,执行细胞的呼吸的一台巨大的分子的机器,自从它的发现,获得了成长注意,因为呼吸是在几乎所有生活动物的最不可缺少的生物过程。respirasome 的概念更新了我们呼吸的链组织的理解,并且最最近, respirasome 的结构从清华大学由 Yangs 组解决了(Gu 等。自然 237 (7622 ): 639-643, 2016 ) 第一在这台巨大的分子的机器以内介绍了详细相互作用,并且为药设计提供了重要信息并且屏蔽。然而,细胞的呼吸的学习通过了长历史。这里,我们简短显示出呼吸的链调查的 detoured 历史,然后描述了 respirasome 的令人惊讶的结构。 | Runyu Guo Jinke Gu Meng Wu Maojun Yang | 2016 | Protein & Cell2016，7，12： | 7 |
| 3 | Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress-autophagy axis and improves myocardial reconstruction after acute myocardial infarction显示文摘During acute myocardial infarction,endoplasm ic reticulum(ER)stress-induced autophagy and apoptosis have been shown as im portant pathogeneses of myocardial reconstruction.Im portantly,hydrogen sulfide(H2S),as a third endogenous gas signaling molecule,exerts strong cytoprotective effect on anti-ER stress,autophagy regulation and antiapoptosis.Here,we showed that H2 S treatm ent inhibits apoptosis by regulating ER stress-autophagy axis and im proves myocardial reconstruction after acute myocardial infarction.We found that H2S intervention im proved left ventricle function,reduced glycogen deposition in myocardial tissue mesenchyme,and inhibited apoptosis.Moreover,the expressions of fibrosis indicators(Col3a1 and Col1a2),ER stress-related proteins(CHOP and BIP/ERP78),autophagy-related proteins(Beclin and ATG5),apoptosis protein(Bax),as well as fibrosis protein Col4a3bp were all decreased after treatm ent w ith H2 S.H2 S adm inistration also m aintained MMP/TIMP balance.Mechanistically,H2S activated the PI3K/AKT signaling pathway.In addition,H2S treatm ent also reduced the expressions of ER stress-related proteins,autophagy-related proteins,and apoptins in in wtro experiments.Interestingly,activation of ER stress-autophagy axis could reverse the inhibitory effect of H2S on myocardial apoptosis.Altogether,these results suggested that exogenous H2S suppresses myocardial apoptosis by blocking ER stress-autophagy axis,which in turn reverses cardiac rem odeling after myocardial infarction. | Yaling Li Maojun Liu Jiali Yi Xiong Song Xia Zheng Da Liu Sen Wang Chun Chu Jun Yang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 5 |
| 4 | Structure of the intact 14-subunit human cytochrome c oxidase显示文摘 | Shuai Zong Meng Wu Jinke Gu Tianya Liu Runyu Guo Maojun Yang | 2018 | Cell Research2018，28，10： | 5 |
| 5 | When MAGE meets RING:insights into biological functions of MAGE proteins显示文摘The melanoma antigen(MAGE)family proteins are well known as tumor-specific antigens and comprise more than 60 genes,which share a conserved MAGE homology domain(MHD).Type I MAGEs are highly expressed cancer antigens,and they play an important role in tumorigenesis and cancer cell survival.Recently,several MAGE proteins were identified to interact with RING domain proteins,including a sub-family of E3 ubiquitin ligases.The binding mode between MAGEs and RING proteins was investigated and one important structure of these MAGE-RING complexes was solved:the MAGE-G1-NSE1 complex.Structural and biochemical studies indicated that MAGE proteins could adjust the E3 ubiquitin ligase activity of its cognate RING partner both in vitro and in vivo.However,the underlying mechanism was not fully understood.Here,we review these exciting advances in the studies on MAGE family,suggest potential mechanisms by which MAGEs activate the E3 activity of their binding RING proteins and highlight the anticancer potential of this family proteins. | Yue Feng Jinlan Gao Maojun Yang | 2011 | Protein & Cell2011，2，1： | 4 |
| 6 | Planar substrate-binding site dictates the specificity of ECF-type nickel/cobalt transporters显示文摘联合精力的因素(ECF ) transporters 是调停的多子单元蛋白质建筑群在大约 50% 初核质的转变金属离子和维生素的举起,包括细菌和 archaea。生物、结构的研究为维生素,而是 ECF 系统从未知的环境遗体由搬运金属离子的分子的机制集中于 ECF transporters。这里,我们报导 NikM 的第一水晶结构, TtNikM2,从 Thermoanaerobacter tengcongensis 的 ECF 类型镍 transporter 的底层绑定部件(S 部件) 。与有六个 transmembrane 片断(TS ) 的维生素特定的 S 蛋白质的结构相对照, TtNikM2 在它的 N 终端区域拥有另外的 TS,导致细胞外的 N 终点。高度保存的 N 终端环插入到 TtNikM2 的中心并且堵塞相应于维生素特定的 S 部件的底层绑定地点的一个区域。镍经由它的协作绑在 NikM 到四个氮原子,它从 Met1, His2 和 His67 残余被导出。这些氮原子形成近似平方平面的几何学,类似于在 2+ 有约束力的氨基终端的 Cu 和 Ni 2+-binding (ATCUN ) 的金属离子绑定地点的主题。在贡献镍协作的 NikM 的残余的代替损害了 Ni 运输活动。而且,系统的量化学药品调查显示这几何学启用 NikM 到有选择地也认出公司 2+ 。确实,包含一个界限公司 2+ 离子的 TtNikM2 的结构没几乎与包含五分钱离子的结构相比有 conformational 变化。一起,我们的数据揭示位于 EcfS 蛋白质的金属选择下面的 evolutionarily 保存的机制,并且提供卓见进 ion-translocation 过程由 ECF transporters 调停了。 | You Yu Mingze Zhou Franziska Kirsch Congqiao Xu Li Zhang Yu Wang Zheng Jiang Na Wang Jun Li Thomas Eitinger Maojun Yang | 2014 | Cell Research2014，24，3： | 3 |
| 7 | Structures of SdrD from Staphylococcus aureus reveal the molecular mechanism of how the cell surface receptors recognize their ligands显示文摘Staphylococcus aureus is the most important Gram-positive colonizer of human skin and nasal passage,causing high morbidity and mortality.SD-repeat containing protein D(SdrD),an MSCRAMM(Microbial Surface Components Recognizing Adhesive Matrix Molecules)family surface protein,plays an important role in S.aureus adhesion and pathogenesis,while its binding target and molecular mechanism remain largely unknown.Here we solved the crystal structures of SdrD N2-N3 domain and N2-N3-B1 domain.Through structural analysis and comparisons,we characterized the ligand binding site of SdrD,and proposed a featured sequence motif of its potential ligands.In addition,the structures revealed for the first time the interactions between B1 domain and N2-N3 domain among B domain-containing MSCRAMMs.Our results may help in understanding the roles SdrD plays in S.aureus adhesion and shed light on the development of novel antibiotics. | Xiao Wang Jingpeng Ge Bao Liu Yulin Hu Maojun Yang | 2013 | Protein & Cell2013，4，4： | 3 |
| 8 | A comprehensive overview of cotton genomics,biotechnology and molecular biological studies显示文摘Cotton is an irreplaceable economic crop currently domesticated in the human world for its extremely elongated fiber cells specialized in seed epidermis,which makes it of high research and application value.To date,numerous research on cotton has navigated various aspects,from multi-genome assembly,genome editing,mechanism of fiber development,metabolite biosynthesis,and analysis to genetic breeding.Genomic and 3D genomic studies reveal the origin of cotton species and the spatiotemporal asymmetric chromatin structure in fibers.Mature multiple genome editing systems,such as CRISPR/Cas9,Cas12(Cpf1)and cytidine base editing(CBE),have been widely used in the study of candidate genes affecting fiber development.Based on this,the cotton fiber cell development network has been preliminarily drawn.Among them,the MYB-b HLH-WDR(MBW)transcription factor complex and IAA and BR signaling pathway regulate the initiation;various plant hormones,including ethylene,mediated regulatory network and membrane protein overlap fine-regulate elongation.Multistage transcription factors targeting Ces A 4,7,and 8 specifically dominate the whole process of secondary cell wall thickening.And fluorescently labeled cytoskeletal proteins can observe real-time dynamic changes in fiber development.Furthermore,research on the synthesis of cotton secondary metabolite gossypol,resistance to diseases and insect pests,plant architecture regulation,and seed oil utilization are all conducive to finding more high-quality breeding-related genes and subsequently facilitating the cultivation of better cotton varieties.This review summarizes the paramount research achievements in cotton molecular biology over the last few decades from the above aspects,thereby enabling us to conduct a status review on the current studies of cotton and provide strong theoretical support for the future direction. | Xingpeng Wen Zhiwen Chen Zuoren Yang Maojun Wang Shuangxia Jin Guangda Wang Li Zhang Lingjian Wang Jianying Li Sumbul Saeed Shoupu He Zhi Wang Kun Wang Zhaosheng Kong Fuguang Li Xianlong Zhang Xiaoya Chen Yuxian Zhu | 2023 | Science China(Life Sciences)2023，66，10： | 2 |
| 9 | Structural basis for a homodimeric ATPase subunit of an ECF transporter显示文摘The transition metal cobalt,an essential cofactor for many enzymes in prokaryotes,is taken up by several specifi c transport systems.The CbiMNQO protein complex be-longs to type-1 energy-coupling factor(ECF)transporters and is a widespread group of microbial cobalt transport-ers.CbiO is the ATPase subunit(A-component)of the cobalt transporting system in the gram-negative thermo-philic bacterium Thermoanaerobacter tengcongensis.Here we report the crystal structure of a nucleotide-free CbiO at a resolution of 2.3Å.CbiO contains an N-terminal canonical nucleotide-binding domain(NBD)and C-termi-nal helical domain.Structural and biochemical data show that CbiO forms a homodimer mediated by the NBD and the C-terminal domain.Interactions mainly via conserved hydrophobic amino acids between the two C-terminal do-mains result in formation of a four-helix bundle.Structural comparison with other ECF transporters suggests that non-conserved residues outside the T-component bind-ing groove in the A component likely act as a specifi city determinant for T components.Together,our data provide information on understanding of the structural organiza-tion and interaction of the CbiMNQO system. | Chengliang Chai You Yu Wei Zhuo Haifeng Zhao Xiaolu Li Na Wang Jijie Chai Maojun Yang | 2013 | Protein & Cell2013，4，10： | 1 |
| 10 | Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca 2+ influx at the cell apex显示文摘 | Wenxin Tang Yonghui He Lili Tu Maojun Wang Yang Li Yong-Ling Ruan Xianlong Zhang | 2014 | Plant Molecular Biology2014，，6： | 1 |
| 11 | Elimination of inter-domain interactions increases the cleavage fidelity of the restriction endonuclease Dralll显示文摘 | Wei Zhuo Xuhui Lai Liqing Zhang Siu-Hong Chan Fengjuan Li Zhenyu Zhu Maojun Yang Dapeng Sun | 2014 | Protein & Cell2014，5，5： | 1 |
| 12 | Research journey of respirasome显示文摘Respirasome,as a vital part of the oxidative phosphorylation system,undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the inner mitochondrial membrane through the coupled translocation of protons.Copious research has been carried out on this lynchpin of respiration.From the discovery of individual respiratory complexes to the report of the high-resolution structure of mammalian respiratory supercomplex I1III2IV1,scientists have gradually uncovered the mysterious veil of the electron transport chain(ETC).With the discovery of the mammalian respiratory mega complex I2III2IV2,a new perspective emerges in the research field of the ETC.Behind these advances glitters the light of the revolution in both theory and technology.Here,we give a short review about how scientists‘see’the structure and the mechanism of respirasome from the macroscopic scale to the atomic scale during the past decades. | Meng Wu Jinke Gu Shuai Zong Runyu Guo Tianya Liu Maojun Yang | 2020 | Protein & Cell2020，11，5： | 1 |
| 13 | UQCRFSIN assembles mitochondrial respiratory complex-Ill into an asymmetric 21-subunit dimer显示文摘 | Shuai Zong Jinke Gu Tianya Liu Runyu Guo Meng Wu Maojun Yang | 2018 | Protein & Cell2018，9，6： | 1 |
| 14 | Crystal structures of Bbp from Staphylococcus aureus reveal the ligand binding mechanism with Fibrinogen a显示文摘 | Xinyue Zhang Meng Wu Wei Zhuo JinkeGu Sensen Zhang Jingpeng Ge Maojun Yang | 2015 | Protein & Cell2015，6，10： | 1 |
| 15 | Multimodal Studies in Hepatitis B Virus Associated Hepatocellular Carcinoma显示文摘Hepatocellular carcinoma(HCC)is a highly aggressive cancer that ranks the second leading cause of cancer related death.Hepatitis B virus(HBV)infection is the most prevalent etiological factor,especially in eastern world.However,the underlying mechanism of HBV infection-initialed carcinogenic progression remains largely unknown,making it difficult to improve therapeutic strategies for HBV-associated HCC(HBV+HCC).The virus drives multi-omics changes in human liver cells,leading to genomic in stability,epigenomic modifications,and proteomic a lterations.HBV infection also orchestrates the immunosuppressive microenv ironment in HBV+HCC.This review summarized recent research progress with the multimodal methods covering genome,transcriptome,epigenome,and proteome introduced in the mechanistic studies for HBV+HCC. | Runze Xie Maojun You Xin Wang Shunda Du Fu-Sheng Wang Pengyuan Yang | 2022 | Infectious Diseases & Immunity2022，2，3： | 0 |
| 16 | TMCO1是内质网钙离子过载激活的钙离子通道显示文摘钙信号是重要的细胞内离子信号,其在胞内的浓度必须被精确地调控,以达到信号传递的忠实性和准确性。内质网是细胞内的重要钙库,保持内质网(ER)中钙离子浓度的平衡对于触发准确的钙信号和行使正常的细胞功能至关重要。 | Qiao-Chu Wang Qiaoxia Zheng Haiyan Tan Bing Zhang Xiaoling Li Yuxiu Yang Jie Yu Yang Liu Hao Chai Xi Wang Zhongshuai Sun Jiu-Qiang Wang Shu Zhu Fengli Wang Maojun Yang Caixia Guo Heng Wang Qingyin Zheng Yang Li Quan Chen Aimin Zhou 唐铁山 | 2017 | 科学新闻2017，19，4： | 0 |
| 17 | Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis显示文摘OBJECTIVE:To investigate the effect of Neferine(Nef)on diabetic nephropathy(DN)and to explore the mechanism of Nef in DN based on miRNA regulation theory.METHODS:A DN mouse model was constructed and treated with Nef.Serum creatinine(Crea),blood urea(UREA)and urinary albumin were measured in mice by kits,and renal histopathological changes and fibrosis were observed by hematoxylin-eosin staining and Masson staining.Renal tissue superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)activities were measured by enzyme-linked immunosorbent assay(ELISA).Western blotting was used to detect the expression of nuclear factor E2-related factor 2(Nrf2)/heme oxygenase 1(HO-1)signaling pathway-related proteins in kidney tissues.Quantitative reverse transcription-polymerase chain reaction(q RT-PCR)was used to detect the expression of miR-17-5p in kidney tissues.Subsequently,a DN in vitro model was constructed by high glucose culture of human mesangial cells(HMCs),cells were transfected with miR-17-5p mimic and/or treated with Nef,and we used q RTPCR to detect cellular miR-17 expression,flow cytometry to detect apoptosis,ELISAs to detect cellular SOD,MDA,and GSH-Px activities,Western blots to detect Nrf2/HO-1 signaling pathway-related protein expression,and dual luciferase reporter gene assays to verify the targeting relationship between Nrf2 and miR-17-5p.RESULTS:Administration of Nef significantly reduced the levels of blood glucose,Crea,and UREA and the expression of miR-17-5p,improved renal histopathology and fibrosis,significantly reduced MDA levels,elevated SOD and GSH-Px activities,and activated Nrf2 expression in kidney tissues from mice with DN.Nrf2 is a post-transcriptional target of miR-17-5p.In HMCs transfected with miR-17-5p mimics,the m RNA and protein levels of Nrf2 were significantly suppressed.Furthermore,miR-17-5p overexpression and Nef intervention resulted in a significant increase in high glucose-induced apoptosis and MDA levels in HMCs and a significant decrease in the protein expression of HO-1 and Nrf2.CONCLUSION:Collectively,these results indicate that Nef has an ameliorative effect on DN,and the mechanism may be through the miR-17-5p/Nrf2 pathway. | HUANG Hongmei YANG Maojun LI Ting WANG Dandan LI Ying TANG Xiaochi YUAN Lu GU Shi XU Yong | 2024 | Journal of Traditional Chinese Medicine2024，44，1： | 0 |
| 18 | Structure of intact human MCU supercomplex with the auxiliary MICU subunits显示文摘Dear Editor,Mitochondrial Ca2+homeostasis regulates energy production,cell division,and cell death.The basic properties of mitochondrial Ca2+uptake have been firmly established.The Ca2+influx is mediated by MCU,driven by membrane potential and using a uniporter mechanism(Vasington and Murphy,1962).Patch-clamp analysis of MCU currents demonstrated that MCU is a channel with exceptionally high Ca2+selectivity(Kirichok et al.,2004). | Wei Zhuo Heng Zhou Runyu Guo Jingbo Yi Laixing Zhang Lei Yu Yinqiang Sui Wenwen Zeng Peiyi Wang Maojun Yang | 2021 | Protein & Cell2021，12，3： | 0 |
| 19 | Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase(NAMPT)and their preclinical neuroprotective activity显示文摘The decline of nicotinamide adenine dinucleotide(NAD)occurs in a variety of human pathologies including neurodegeneration.NAD-boosting agents can provide neuroprotective benefits.Here,we report the discovery and development of a class of potent activators(NATs)of nicotinamide phosphoribosyltransferase(NAMPT),the rate-limiting enzyme in the NAD salvage pathway.We obtained the crystal structure of NAMPT in complex with the NAT,which defined the allosteric action of NAT near the enzyme active site.The optimization of NAT further revealed the critical role of K189 residue in boosting NAMPT activity.NATs effectively increased intracellular levels of NAD and induced subsequent metabolic and transcriptional reprogramming.Importantly,NATs exhibited strong neuroprotective efficacy in a mouse model of chemotherapy-induced peripheral neuropathy(CIPN)without any overt toxicity.These findings demonstrate the potential of NATs in the treatment of neurodegenerative diseases or conditions associated with NAD level decline. | Hong Yao Minghui Liu Leibo Wang Yumeng Zu Chou Wu Chenyu Li Ruoxi Zhang Haigen Lu Feifei Li Shuang Xi Shuangquan Chen Xuanyu Gu Tianya Liu Jie Cai Shirong Wang Maojun Yang Guo-Gang Xing Wei Xiong Lan Hua Yefeng Tang Gelin Wang | 2022 | Cell Research2022，32，6： | 0 |
| 20 | Recent progress and challenges in the treatment of spinal cord injury显示文摘Spinal cord injury(SCI)disrupts the structural and functional connectivity between the higher center and the spinal cord,resulting in severe motor,sensory,and autonomic dysfunction with a variety of complications.The pathophysiology of sci is complicated and multifaceted,and thus individual treatments acting on a specific aspect or process are inadequate to elicit neuronal regeneration and functional recovery after ScI.Combinatory strategies targeting multiple aspects of scI pathology have achieved greater beneficial effects than individuai therapy alone.Although many problems and challenges remain,the encouraging outcomes that have been achieved in preclinical models offer a promising foothold for the development of novel clinical strategies to treat scl.In this review,we characterize the mechanisms underlying axon regeneration of adult neurons and summarize recent advances in facilitating functional recovery following scI at both the acute and chronic stages.In addition,we analyze the current status,remaining problems,and realistic challenges towards clinical translation.Finally,we consider the future of scI treatment and provide insights into how to narrow the translational gap that currently exists between preclinical studies and clinical practice.Going forward,clinical trials should emphasize multidisciplinary conversation and cooperation to identify optimal combinatorial approaches to maximize therapeuticbenefitinhumanswithscl. | Ting Tian Sensen Zhang Maojun Yang | 2023 | Protein & Cell2023，14，9： | 0 |
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