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| 1 | Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Brassica rapa comprises several important cultivated vegetables and oil crops.Current reference genome assemblies of Brassica rapa are quite fragmented and not highly contiguous,thereby limiting extensive genetic and genomic analyses.Here,we report an improved assembly of the B.rapa genome(v3.0)using single-molecule sequencing,optical mapping,and chromosome conformation capture technologies(Hi-C).Relative to the previous reference genomes,our assembly features a contig N50 size of 1.45 Mb,representing a~30-fold improvement.We also identified a new event that occurred in the B.rapa genome~1.2 million years ago,when a long terminal repeat retrotransposon(LTR-RT)expanded.Further analysis refined the relationship of genome blocks and accurately located the centromeres in the B.rapa genome.The B.rapa genome v3.0 will serve as an important community resource for future genetic and genomic studies in B.rapa.This resource will facilitate breeding efforts in B.rapa,as well as comparative genomic analysis with other Brassica species. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 10 |
| 2 | Branched tetraether lipids in Chinese soils:Evaluating the fidelity of MBT/CBT proxies as paleoenvironmental proxies显示文摘The ubiquitous occurrence of branched glycerol dialkyl glycerol tetraethers(br GDGTs) in soils has allowed development of new proxies for reconstruction of past climate and environment. The methylation and cyclization degrees of br GDGTs, expressed as MBT and CBT, respectively, are reported to be mainly controlled by mean annual air temperature(MAAT) and soil p H. However, the br GDGT-derived temperatures and soil p H scatter widely when data from different environmental conditions are considered. In this study, we collected over 300 soil samples from China, which are representative of humid(Xishuangbanna, Guangzhou, and Shanghai), semi-arid(Dongying) and semi-arid/arid(Lanzhou, Tibetan Plateau) regions. Collectively we have the most extensive dataset that broadly characterizes the distribution of br GDGTs according to climate zones in China. The overall data demonstrate that the MBT/CBT derived temperatures better match the measured MAATs in humid and non-alkaline regions than those from regions of low MAP(<400 mm/yr) and above neutral soil p H(>7.0–7.5). Similarly, CBT describes soil p H much better in humid and non-alkaline soils than in semi-arid/arid and alkaline soils; the semi-arid/arid and alkaline soils tend to show a positive correlation between soil p H and CBT, which contradicts that in the humid and non-alkaline soils. While soil p H, MAAT and mean annual precipitation(MAP) are dominating factors controlling the br GDGT distribution across all climate zones, conductivity, total organic carbon and total nitrogen, as well as soil water content can also play an important role locally. Removing br GDGT-II resulted in a revised CBT index that provides more accurate estimation of p H, especially in semi-arid/arid and alkaline soils. The overall Chinese dataset demonstrates that continental air temperature derived from br GDGT-proxies can vastly deviate from real measurements and should be used with extreme caution in paleo-climate or-environment studies. | ZHENG FengFeng ZHANG ChuanLun CHEN YuFei LI FuYan MA CenLing PU Yang ZHU YuanQing WANG YongLi LIU WeiGuo | 2016 | Science China Earth Sciences2016，59，7： | 10 |
| 3 | Comparison of the short-term and long-term outcomes of laparoscopic hysterectomies and of abdominal hysterectomies: a case study of 4,895 patients in the Guangxi Zhuang Autonomous Region, China显示文摘Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy(LH) compared with abdominal hysterectomy(AH) in case of benign gynecological disease. Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies(3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time(OT), estimated blood loss(EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed.Results: The EBL(174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus(38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter(1.5±0.6 vs. 2.2±0.8 days), use of analgesics(6.5% vs. 73.1%), intra-operative complication rate(2.4% vs. 4.1%), post-operative complication rate(2.3% vs. 5.7%), post-operative constipation(12.1% vs. 24.6%), mild and serious stress urinary incontinence(SUI) post-operative(P<0.001; P=0.014), and proportion of Female Sexual Functioning Index(FSFI) total score <26.55 post-operative(P<0.001) of the LH group were significantly less than those of AH group. There wereno significant differences in OT(106.5±34.5 vs. 106.2±40.3 min) between the two groups.Conclusions: LH is a safe and efficient operation for improving patients' long-term quality of life(Qo L), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus. | Hongying He Zhijun Yang Dingyuan Zeng Jiangtao Fan Xiaoxia Hu Yuan Ye Hua Bai YanmingJiang Zhong Lin Zhiying Lei Xinlin Li Lian Li Jinghua Gan Ying Lan Xiongzhi Tang Danxia Wang Junsong Jiang Xiaoyan Wu Meiying Li Xiaoqing Ren Xiaomin Yang Mei Liu Qinmei Wang Fuyan jiang Li Li | 2016 | Chinese Journal of Cancer Research2016，28，2： | 6 |
| 4 | Biogenic synthesis of silver nanoparticles using ginger(Zingiber officinale)extract and their antibacterial properties against aquatic pathogens显示文摘With the development of aquaculture, there is an urgent demand for an alternative antibacterial agent to reduce the drug resistance and environmental pollution caused by the abuse of antibiotics. Recently, silver nanoparticles(Ag NPs) have been viewed as a novel type of antimicrobial agents due to their unique advantages. In this study,Ag NPs were biosynthesized with the ginger rhizomes extract. The biosynthesized Ag NPs were characterised by UV–visible spectroscopy, transmission electron microscopy, X-ray diffraction and fourier transform infrared spectroscopy. Furthermore, the antimicrobial activities of the Ag NPs were fully analyzed against six typical aquatic pathogens. The results indicated that the components in ginger extract could function as the chemical reductant to synthesize Ag NPs. Moreover, compared with the Ag NPs synthesized by chemical methods, the biosynthesized Ag NPs were smaller, and had higher stability and antibacterial activity. Therefore, the biosynthesized Ag NPs using ginger extract may have prospective applications in aquaculture. | YANG Nan LI Fuyan JIAN Tiancai LIU Chongchong SUN Hushan WANG Lei XU Hui | 2017 | Acta Oceanologica Sinica2017，36，12： | 4 |
| 5 | Circular RNA circCCNB1 inhibits the migration and invasion of nasopharyngeal carcinoma through binding and stabilizing TJP1 mRNA显示文摘Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(circ RNAs)exacerbate the process of metastasis in NPC;however,their underlying mechanisms remain unclear.We found that the circular RNA circ CCNB1,encoded by the oncogene CCNB1,was downregulated in NPC biopsies and cell lines.In vitro assays show that circ CCNB1 inhibits NPC cell migration and invasion.Moreover,circ CCNB1 induces a protein,nuclear factor 90(NF90),to bind and prolong the half-life of tight junction protein 1(TJP1)m RNA.Upregulation of TJP1 enhances tight junctions between cancer cells and inhibits NPC cell migration and invasion.This study reveals a novel biological function of circ CCNB1 in the migration and invasion of NPC by enhancing the tight junctions of cancer cells by binding to NF90 proteins and TJP1 m RNA,and may provide a potential therapeutic target for NPC. | Mengyao Zhao Yian Wang Fenghua Tan Lingyun Liu Xiangchan Hou Chunmei Fan Le Tang Yongzhen Mo Yumin Wang Qijia Yan Zhaojian Gong Zheng Li Qianjin Liao Can Guo He Huang Xi Zeng Guiyuan Li Zhaoyang Zeng Wei Xiong Fuyan Wang | 2022 | Science China(Life Sciences)2022，65，11： | 4 |
| 6 | Author Correction:Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Since the publication of this article,the authors have noticed that the total gene models(45,985),tandem arrays(2077),tandem genes(4963),redundancy removed(43,099),syntenic genes(39,858),nonsyntenic genes(3241),genes on chromosomes(45,411),genes on scaffolds(574)of B.rapa reference genome v3.0 were mistaken in the article. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2019 | Horticulture Research2019，6，1： | 2 |
| 7 | Reproductive tissue-specific translatome of a rice thermo-sensitive genic male sterile line显示文摘Translational regulation,especially tissue-or cell type-specific gene regulation,plays essential roles in plant growth and development.Thermo-sensitive genic male sterile(TGMS)lines have been widely used for hybrid breeding in rice(Oryza sativa).However,little is known about translational regulation during reproductive stage in TGMS rice.Here,we use translating ribosome affinity purification(TRAP)combined with RNA sequencing to investigate the reproductive tissue-specific translatome of TGMS rice expressing FLAG-tagged ribosomal protein L18(RPL18)from the germline-specific promoter MEIOSIS ARRESTED AT LEPTOTENE1(MEL1).Differentially expressed genes at the transcriptional and translational levels are enriched in pollen and anther-related formation and development processes.These contain a number of genes reported to be involved in tapetum programmed cell death(PCD)and lipid metabolism during pollen development and anther dehiscence in rice,including several encoding transcription factors and key enzymes,as well as several long non-coding RNAs(lnc RNAs)that potentially affect tapetum and pollenrelated genes in male sterility.This study represents the comprehensive reproductive tissue-specific characterization of the translatome in TGMS rice.These results contribute to our understanding of the molecular basis of sterility in TGMS rice and will facilitate further genetic manipulation of TGMS rice in two-line breeding systems. | Wei Liu Jing Sun Ji Li Chunyan Liu Fuyan Si Bin Yan Zhen Wang Xianwei Song Yuanzhu Yang Yuxian Zhu Xiaofeng Cao | 2022 | Journal of Genetics and Genomics2022，49，7： | 1 |
| 8 | Fuzzy control of a double-inverted pendulum显示文摘 | FUYAN CHENG GUOMIN ZHONG YOUSHAN LI | 1996 | Fuzzy Setsand Systems1996，79，3： | 1 |
| 9 | A novel image encryption scheme based on spatial chaos map 显示文摘 | SUN Fuyan LIU Shutang LI Zhongqin | 2008 | Chaos Solitons & Fractals2008，38，3： | 1 |
| 10 | Fuzzy Control of a Double-inverted Pendulum显示文摘 | Cheng Fuyan Zhong Guomin Li Youshan | 1996 | Fuzzy Sets and Systems1996，79，3： | 1 |
| 11 | A novel im- age encryption scheme based on spatial chaos map显示文摘 | SUN FUYAN LIU SHUTANG LI ZHONGQING | 2008 | Chaos Soli- tons & Fractals2008，38，3： | 1 |
| 12 | A Novel Image Encryption Scheme Based on Spatial Chaos Map显示文摘 | Sun Fuyan Liu Shutang Li Zhongqin | 2008 | Chaos Solitons & Fractals2008，38，3： | 1 |
| 13 | Fuzzy Control of a Double-inverted Pendulum 显示文摘 | Fuyan Cheng Guomin Zhong Youshan Li | 1996 | Fuzzy Sets and Systems1996，79，： | 1 |
| 14 | Deletion of neuronal NOS prevents impaired vasodilation in septic mouse skeletal muscle 显示文摘 | Darcy Lidington Fuyan Li | 2007 | Cardiovascular Res2007，，74： | 1 |
| 15 | Study on Antibacterial Efficacy of Plant-Derived Compound Preservative and Its Product Application显示文摘Natural antiseptic formulas are increasingly favored by consumers. This article take preservatives compounded from cinnamon, sophora flavescens and other plant extracts as the main research object, evaluated its antibacterial efficacy and application in cosmetics, expect that it can be used in cosmetics to replace conventional chemical preservatives. Research on its antibacterial efficacy through inhibition zone and MIC experiment, and product application research used anti-corrosion challenge and antibacterial rate experiment. The results showed that the plant-derived antiseptic system had a broad antibacterial spectrum, inhibition of 10 different common pathogenic bacteria, and had a very significant inhibitory effect on Gram-positive bacteria and fungi;Among them, the diameter of the antibacterial circle for Bacillus subtilis and Aspergillus niger can reached 31.89 mm and 28.26 mm, while the MIC value was 0.4 mg/mL and 0.8 mg/mL. The spray product with 1.5% addition amount successfully passed the anti-corrosion challenge test and meets the anti-corrosion standard requirements;The 2% added amount of disposable gel product has an inhibitory rate of 51.05% and 70.74% against Escherichia coli and Staphylococcus aureus within 20 minutes of action. In summary, the plantderived compound preservative has ability to be used in cosmetic preservative systems. | Dai Zhaoyang Hu Liyun Li Fuyan Ai Yong Zhang Wenyun Zhang Bing | 2023 | China Detergent & Cosmetics2023，8，1： | 0 |
| 16 | MANF brakes TLR4 signaling by competitively binding S100A8 with S100A9 to regulate macrophage phenotypes in hepatic fibrosis显示文摘The mesencephalic astrocyte-derived neurotrophic factor(MANF)has been recently identified as a neurotrophic factor,but its role in hepatic fibrosis is unknown.Here,we found that MANF was upregulated in the fibrotic liver tissues of the patients with chronic liver diseases and of mice treated with CCl4.MANF deficiency in either hepatocytes or hepatic mono-macrophages,particularly in hepatic mono-macrophages,clearly exacerbated hepatic fibrosis.Myeloid-specific MANF knockout increased the population of hepatic Ly6C^(high)macrophages and promoted HSCs activation.Furthermore,MANF-sufficient macrophages(from WT mice)transfusion ameliorated CCl4-induced hepatic fibrosis in myeloid cells-specific MANF knockout(MKO)mice.Mechanistically,MANF interacted with S100A8 to competitively block S100A8/A9 heterodimer formation and inhibited S100A8/A9-mediated TLR4-NF-κB signal activation.Pharmacologically,systemic administration of recombinant human MANF significantly alleviated CCl_(4)-induced hepatic fibrosis in both WT and hepatocytes-specific MANF knockout(HKO)mice.This study reveals a mechanism by which MANF targets S100A8/A9-TLR4 as a“brake”on the upstream of NF-κB pathway,which exerts an impact on macrophage differentiation and shed light on hepatic fibrosis treatment. | Chao Hou Dong Wang Mingxia Zhao Petek Ballar Xinru Zhang Qiong Mei Wei Wang Xiang Li Qiang Sheng Jun Liu Chuansheng Wei Yujun Shen Yi Yang Peng Wang Juntang Shao Sa Xu Fuyan Wang Yang Sun Yuxian Shen | 2023 | Acta Pharmaceutica Sinica B2023，13，10： | 0 |
| 17 | Design of a high-sampling-rate electronic module for array-detector positron annihilation lifetime measurements显示文摘Introduction In this study,a high-time-resolution electronic module with a high channel density and low power consumption was designed for the measurement of the multi-detector array positron annihilation lifetimes.This electronic module consisted of 32 input channels,and each channel provided a high sampling rate up to 5.12 GSPS based on a Domino Ring Sampler 4(DRS4)chip.Compared to the high-speed flash analog-digital converter(FADC),DRS4 chip has a higher channel density with an affordable lower price and power consumption.Methods The developed electronic module was also capable of real-time data analysis for directly extracting the time information of input signals at the data acquisition site,thereby significantly decreasing the data rate.The digital constant fraction discriminator(DCFD)algorithm was implemented in the field programmable gate array(FPGA)for performing the time pick-up.Results The coincidence time resolution of the electronic module was measured,and the test results revealed a value of 26 ps.A prototypical 16-pixel detector module of the multi-detector system was evaluated using this electronic module,and the coincidence time resolution of the prototypical module was 411.84 ps.Conclusions The electronic module was confirmed to satisfy the severe requirements of the multi-array-detector positron annihilation lifetime measurement system.It was also suitable for other high-time-resolution,high-channel-density,costeffective,and low-power-consumption applications. | Jiale Cai Daowu Li Yingjie Wang Zhiming Zhang Peilin Wang Haohui Tang Baoyi Wang Xingzhong Cao Fuyan Liu Long Wei | 2019 | Radiation Detection Technology and Methods2019，3，3： | 0 |
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