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| 1 | Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Brassica rapa comprises several important cultivated vegetables and oil crops.Current reference genome assemblies of Brassica rapa are quite fragmented and not highly contiguous,thereby limiting extensive genetic and genomic analyses.Here,we report an improved assembly of the B.rapa genome(v3.0)using single-molecule sequencing,optical mapping,and chromosome conformation capture technologies(Hi-C).Relative to the previous reference genomes,our assembly features a contig N50 size of 1.45 Mb,representing a~30-fold improvement.We also identified a new event that occurred in the B.rapa genome~1.2 million years ago,when a long terminal repeat retrotransposon(LTR-RT)expanded.Further analysis refined the relationship of genome blocks and accurately located the centromeres in the B.rapa genome.The B.rapa genome v3.0 will serve as an important community resource for future genetic and genomic studies in B.rapa.This resource will facilitate breeding efforts in B.rapa,as well as comparative genomic analysis with other Brassica species. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 10 |
| 2 | Branched tetraether lipids in Chinese soils:Evaluating the fidelity of MBT/CBT proxies as paleoenvironmental proxies显示文摘The ubiquitous occurrence of branched glycerol dialkyl glycerol tetraethers(br GDGTs) in soils has allowed development of new proxies for reconstruction of past climate and environment. The methylation and cyclization degrees of br GDGTs, expressed as MBT and CBT, respectively, are reported to be mainly controlled by mean annual air temperature(MAAT) and soil p H. However, the br GDGT-derived temperatures and soil p H scatter widely when data from different environmental conditions are considered. In this study, we collected over 300 soil samples from China, which are representative of humid(Xishuangbanna, Guangzhou, and Shanghai), semi-arid(Dongying) and semi-arid/arid(Lanzhou, Tibetan Plateau) regions. Collectively we have the most extensive dataset that broadly characterizes the distribution of br GDGTs according to climate zones in China. The overall data demonstrate that the MBT/CBT derived temperatures better match the measured MAATs in humid and non-alkaline regions than those from regions of low MAP(<400 mm/yr) and above neutral soil p H(>7.0–7.5). Similarly, CBT describes soil p H much better in humid and non-alkaline soils than in semi-arid/arid and alkaline soils; the semi-arid/arid and alkaline soils tend to show a positive correlation between soil p H and CBT, which contradicts that in the humid and non-alkaline soils. While soil p H, MAAT and mean annual precipitation(MAP) are dominating factors controlling the br GDGT distribution across all climate zones, conductivity, total organic carbon and total nitrogen, as well as soil water content can also play an important role locally. Removing br GDGT-II resulted in a revised CBT index that provides more accurate estimation of p H, especially in semi-arid/arid and alkaline soils. The overall Chinese dataset demonstrates that continental air temperature derived from br GDGT-proxies can vastly deviate from real measurements and should be used with extreme caution in paleo-climate or-environment studies. | ZHENG FengFeng ZHANG ChuanLun CHEN YuFei LI FuYan MA CenLing PU Yang ZHU YuanQing WANG YongLi LIU WeiGuo | 2016 | Science China Earth Sciences2016，59，7： | 10 |
| 3 | Comparison of the short-term and long-term outcomes of laparoscopic hysterectomies and of abdominal hysterectomies: a case study of 4,895 patients in the Guangxi Zhuang Autonomous Region, China显示文摘Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy(LH) compared with abdominal hysterectomy(AH) in case of benign gynecological disease. Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies(3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time(OT), estimated blood loss(EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed.Results: The EBL(174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus(38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter(1.5±0.6 vs. 2.2±0.8 days), use of analgesics(6.5% vs. 73.1%), intra-operative complication rate(2.4% vs. 4.1%), post-operative complication rate(2.3% vs. 5.7%), post-operative constipation(12.1% vs. 24.6%), mild and serious stress urinary incontinence(SUI) post-operative(P<0.001; P=0.014), and proportion of Female Sexual Functioning Index(FSFI) total score <26.55 post-operative(P<0.001) of the LH group were significantly less than those of AH group. There wereno significant differences in OT(106.5±34.5 vs. 106.2±40.3 min) between the two groups.Conclusions: LH is a safe and efficient operation for improving patients' long-term quality of life(Qo L), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus. | Hongying He Zhijun Yang Dingyuan Zeng Jiangtao Fan Xiaoxia Hu Yuan Ye Hua Bai YanmingJiang Zhong Lin Zhiying Lei Xinlin Li Lian Li Jinghua Gan Ying Lan Xiongzhi Tang Danxia Wang Junsong Jiang Xiaoyan Wu Meiying Li Xiaoqing Ren Xiaomin Yang Mei Liu Qinmei Wang Fuyan jiang Li Li | 2016 | Chinese Journal of Cancer Research2016，28，2： | 6 |
| 4 | Biogenic synthesis of silver nanoparticles using ginger(Zingiber officinale)extract and their antibacterial properties against aquatic pathogens显示文摘With the development of aquaculture, there is an urgent demand for an alternative antibacterial agent to reduce the drug resistance and environmental pollution caused by the abuse of antibiotics. Recently, silver nanoparticles(Ag NPs) have been viewed as a novel type of antimicrobial agents due to their unique advantages. In this study,Ag NPs were biosynthesized with the ginger rhizomes extract. The biosynthesized Ag NPs were characterised by UV–visible spectroscopy, transmission electron microscopy, X-ray diffraction and fourier transform infrared spectroscopy. Furthermore, the antimicrobial activities of the Ag NPs were fully analyzed against six typical aquatic pathogens. The results indicated that the components in ginger extract could function as the chemical reductant to synthesize Ag NPs. Moreover, compared with the Ag NPs synthesized by chemical methods, the biosynthesized Ag NPs were smaller, and had higher stability and antibacterial activity. Therefore, the biosynthesized Ag NPs using ginger extract may have prospective applications in aquaculture. | YANG Nan LI Fuyan JIAN Tiancai LIU Chongchong SUN Hushan WANG Lei XU Hui | 2017 | Acta Oceanologica Sinica2017，36，12： | 4 |
| 5 | Circular RNA circCCNB1 inhibits the migration and invasion of nasopharyngeal carcinoma through binding and stabilizing TJP1 mRNA显示文摘Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(circ RNAs)exacerbate the process of metastasis in NPC;however,their underlying mechanisms remain unclear.We found that the circular RNA circ CCNB1,encoded by the oncogene CCNB1,was downregulated in NPC biopsies and cell lines.In vitro assays show that circ CCNB1 inhibits NPC cell migration and invasion.Moreover,circ CCNB1 induces a protein,nuclear factor 90(NF90),to bind and prolong the half-life of tight junction protein 1(TJP1)m RNA.Upregulation of TJP1 enhances tight junctions between cancer cells and inhibits NPC cell migration and invasion.This study reveals a novel biological function of circ CCNB1 in the migration and invasion of NPC by enhancing the tight junctions of cancer cells by binding to NF90 proteins and TJP1 m RNA,and may provide a potential therapeutic target for NPC. | Mengyao Zhao Yian Wang Fenghua Tan Lingyun Liu Xiangchan Hou Chunmei Fan Le Tang Yongzhen Mo Yumin Wang Qijia Yan Zhaojian Gong Zheng Li Qianjin Liao Can Guo He Huang Xi Zeng Guiyuan Li Zhaoyang Zeng Wei Xiong Fuyan Wang | 2022 | Science China(Life Sciences)2022，65，11： | 4 |
| 6 | Biology and pathology of the uterine micro environ merit and its natural killer cells显示文摘Tissues are the new frontier of discoveries in immunology.Cells of the immune system are an integral part of tissue physiology and immunity.Determining how immune cells inhabit,housekeep,and defend gut,lung,brain,liver,uterus,and other organs helps revealing the intimate details of tissue physiology and may offer new therapeutic targets to treat pathologies.The uterine microenvironment modulates the development and function of innate lymphoid cells[ILC,largely represented by natural killer(NK)cells],macrophages,T cells,and dendritic cells.These immune cells,in turn,contribute to tissue homeostasis.Regulated by ovarian hormones,the human uterine mucosa(endometrium)undergoes ~400 monthly cycles of breakdown and regeneration from menarche to menopause,with its fibroblasts,glands,blood vessels,and immune cells remodeling the tissue into the transient decidua.Even more transformative changes occur upon blastocyst implantation.Before the placenta is formed,the endometrial glands feed the embryo by histiotrophic nutrition while the uterine spiral arteries are stripped of their endothelial layer and smooth muscle actin.This arterial remodeling is carried out by invading fetal trophoblast and maternal immune cells,chiefly uterine NK(uNK)cells,which also assist fetal growth.The tran sformed arteries no Ion ger resp ond to mater nal stimuli and meet the increasi ng dema nds of the growing fetus.This review focuses on how the everchanging uterine microenvironment affects uNK cells and how uNK cells regulate homeostasis of the decidua,placenta development,and fetal growth.Determining these pathways will help understand the causes of major pregnancy complications. | Fuyan Wang Anita Ellen Qualls Laia Marques-Fernandez Francesco Colucci | 2021 | Cellular & Molecular Immunology2021，18，9： | 2 |
| 7 | Effects of the physical and chemical properties of petroleum coke on its slurryability显示文摘In this study, the effects of particle size distribution (PSD), chemical composition and pore structure of petroleum coke on the slurryability of petroleum coke water slurry (PCWS) were investigated. Four petroleum cokes were studied, and they showed completely different slurryability. The solid concentration at fixed viscosity (i.e. apparent viscosity of 1000 mPa) (SCFV) of four PCWSs is different from each other, with the highest value of 70.9%, and lowest of 62.1%. The apparent viscosity of the four PCWSs all increased with an increase of the solid concentration. The results showed that the PSD, inherent moisture content, specific surface area and pore volume of petroleum coke were key factors to affect the slurryability. The slurryability was enhanced with increasing PSD range and particle packing fraction, and with decreasing inherent moisture content, specific surface area and pore volume. | Gao Fuyan Liu Jianzhong Wang Chuancheng Zhou Junhu Cen Kefa | 2012 | Petroleum Science2012，9，2： | 2 |
| 8 | Author Correction:Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Since the publication of this article,the authors have noticed that the total gene models(45,985),tandem arrays(2077),tandem genes(4963),redundancy removed(43,099),syntenic genes(39,858),nonsyntenic genes(3241),genes on chromosomes(45,411),genes on scaffolds(574)of B.rapa reference genome v3.0 were mistaken in the article. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2019 | Horticulture Research2019，6，1： | 2 |
| 9 | Reproductive tissue-specific translatome of a rice thermo-sensitive genic male sterile line显示文摘Translational regulation,especially tissue-or cell type-specific gene regulation,plays essential roles in plant growth and development.Thermo-sensitive genic male sterile(TGMS)lines have been widely used for hybrid breeding in rice(Oryza sativa).However,little is known about translational regulation during reproductive stage in TGMS rice.Here,we use translating ribosome affinity purification(TRAP)combined with RNA sequencing to investigate the reproductive tissue-specific translatome of TGMS rice expressing FLAG-tagged ribosomal protein L18(RPL18)from the germline-specific promoter MEIOSIS ARRESTED AT LEPTOTENE1(MEL1).Differentially expressed genes at the transcriptional and translational levels are enriched in pollen and anther-related formation and development processes.These contain a number of genes reported to be involved in tapetum programmed cell death(PCD)and lipid metabolism during pollen development and anther dehiscence in rice,including several encoding transcription factors and key enzymes,as well as several long non-coding RNAs(lnc RNAs)that potentially affect tapetum and pollenrelated genes in male sterility.This study represents the comprehensive reproductive tissue-specific characterization of the translatome in TGMS rice.These results contribute to our understanding of the molecular basis of sterility in TGMS rice and will facilitate further genetic manipulation of TGMS rice in two-line breeding systems. | Wei Liu Jing Sun Ji Li Chunyan Liu Fuyan Si Bin Yan Zhen Wang Xianwei Song Yuanzhu Yang Yuxian Zhu Xiaofeng Cao | 2022 | Journal of Genetics and Genomics2022，49，7： | 1 |
| 10 | Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing DNA in BALB/c mice显示文摘 | Meizhen Qiu Fang Fang Yan Chen Hualin Wang Quanjiao Chen Haiyan Chang Fuyan Wang Hanzhong Wang Ran Zhang Ze Chen | 2006 | Biochemical and Biophysical Research Communications2006，，4： | 1 |
| 11 | The slurrying properties of slurry fuels made of petroleum coke and petrochemical sludge显示文摘 | Wang Ruikun Liu Jianzhong Gao Fuyan | 2012 | Fuel Processing Technology2012，，104： | 1 |
| 12 | MANF brakes TLR4 signaling by competitively binding S100A8 with S100A9 to regulate macrophage phenotypes in hepatic fibrosis显示文摘The mesencephalic astrocyte-derived neurotrophic factor(MANF)has been recently identified as a neurotrophic factor,but its role in hepatic fibrosis is unknown.Here,we found that MANF was upregulated in the fibrotic liver tissues of the patients with chronic liver diseases and of mice treated with CCl4.MANF deficiency in either hepatocytes or hepatic mono-macrophages,particularly in hepatic mono-macrophages,clearly exacerbated hepatic fibrosis.Myeloid-specific MANF knockout increased the population of hepatic Ly6C^(high)macrophages and promoted HSCs activation.Furthermore,MANF-sufficient macrophages(from WT mice)transfusion ameliorated CCl4-induced hepatic fibrosis in myeloid cells-specific MANF knockout(MKO)mice.Mechanistically,MANF interacted with S100A8 to competitively block S100A8/A9 heterodimer formation and inhibited S100A8/A9-mediated TLR4-NF-κB signal activation.Pharmacologically,systemic administration of recombinant human MANF significantly alleviated CCl_(4)-induced hepatic fibrosis in both WT and hepatocytes-specific MANF knockout(HKO)mice.This study reveals a mechanism by which MANF targets S100A8/A9-TLR4 as a“brake”on the upstream of NF-κB pathway,which exerts an impact on macrophage differentiation and shed light on hepatic fibrosis treatment. | Chao Hou Dong Wang Mingxia Zhao Petek Ballar Xinru Zhang Qiong Mei Wei Wang Xiang Li Qiang Sheng Jun Liu Chuansheng Wei Yujun Shen Yi Yang Peng Wang Juntang Shao Sa Xu Fuyan Wang Yang Sun Yuxian Shen | 2023 | Acta Pharmaceutica Sinica B2023，13，10： | 0 |
| 13 | Design of a high-sampling-rate electronic module for array-detector positron annihilation lifetime measurements显示文摘Introduction In this study,a high-time-resolution electronic module with a high channel density and low power consumption was designed for the measurement of the multi-detector array positron annihilation lifetimes.This electronic module consisted of 32 input channels,and each channel provided a high sampling rate up to 5.12 GSPS based on a Domino Ring Sampler 4(DRS4)chip.Compared to the high-speed flash analog-digital converter(FADC),DRS4 chip has a higher channel density with an affordable lower price and power consumption.Methods The developed electronic module was also capable of real-time data analysis for directly extracting the time information of input signals at the data acquisition site,thereby significantly decreasing the data rate.The digital constant fraction discriminator(DCFD)algorithm was implemented in the field programmable gate array(FPGA)for performing the time pick-up.Results The coincidence time resolution of the electronic module was measured,and the test results revealed a value of 26 ps.A prototypical 16-pixel detector module of the multi-detector system was evaluated using this electronic module,and the coincidence time resolution of the prototypical module was 411.84 ps.Conclusions The electronic module was confirmed to satisfy the severe requirements of the multi-array-detector positron annihilation lifetime measurement system.It was also suitable for other high-time-resolution,high-channel-density,costeffective,and low-power-consumption applications. | Jiale Cai Daowu Li Yingjie Wang Zhiming Zhang Peilin Wang Haohui Tang Baoyi Wang Xingzhong Cao Fuyan Liu Long Wei | 2019 | Radiation Detection Technology and Methods2019，3，3： | 0 |
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