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| 1 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 2 | Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes显示文摘Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication. | Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong | 2022 | Molecular Plant2022，15，2： | 5 |
| 3 | MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean显示文摘Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding. | Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li | 2021 | Science China(Life Sciences)2021，64，9： | 4 |
| 4 | Generation of male-sterile soybean lines with the CRISPR/Cas9 system显示文摘Soybean [Glycine max(L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to crop improvement. Although genetically modified soybeans have achieved commercial success,hybrid soybean breeding is also a potential way to increase soybean yield. Soybean cytoplasmic malesterile(CMS) lines have been used in three-line hybrid breeding systems, but their application to exploiting soybean heterosis has been limited by rare germplasm resource of sterile lines. The generation of various genetic diversity male-sterile soybean lines will help to overcome the shortcoming. In this study,we used targeted editing of AMS homologs in soybean by CRISPR/Cas9 technology for the first time to generate stable male-sterile lines. Targeted editing of GmAMS1 resulted in a male-sterile phenotype,while editing of GmAMS2 failed to produce male-sterile lines. GmAMS1 functions not only in the formation of the pollen wall but also in the controlling the degradation of the soybean tapetum.CRISPR/Cas9 technology could be used to rapidly produce stable male-sterile lines, providing new sterile-line materials for soybean hybrid breeding systems. | Xiao Chen Suxin Yang Yaohua Zhang Xiaobin Zhu Xinjing Yang Chunbao Zhang Haiyan Li Xianzhong Feng | 2021 | The Crop Journal2021，9，6： | 3 |
| 5 | Changes in meat quality of ovine longissimus dorsi muscle in response to repeated freeze and thaw显示文摘 | Jun Qi Chunbao Li Yinji Chen Feifei Gao Xinglian Xu Guanghong Zhou | 2012 | Meat Science2012，，4： | 2 |
| 6 | MALE STERILITY 3 encodes a plant homeodomain-finger protein for male fertility in soybean显示文摘Male-sterile plants are used in hybrid breeding to improve yield in soybean(Glycine max(L.)Merr.).Developing the capability to alter fertility under different environmental conditions could broaden germplasm resources and simplify hybrid production.However,molecular mechanisms potentially underlying such a system in soybean were unclear.Here,using positional cloning,we identified a gene,MALE STERILITY 3(MS3),which encodes a nuclear-localized protein containing a plant homeodomain(PHD)-finger domain.A spontaneous mutation in ms3 causing premature termination of MS3 translation and partial loss of the PHD-finger.Transgenetic analysis indicated that MS3 knockout resulted in nonfunctional pollen and no selfpollinated pods,and RNA-seq analysis revealed that MS3 affects the expression of genes associated with carbohydrate metabolism.Strikingly,the fertility of mutant ms3 can restore under longd conditions.The mutant could thus be used to create a new,more stable photoperiod-sensitive genic male sterility line for two-line hybrid seed production,with significant impact on hybrid breeding and production. | Jingjing Hou Weiwei Fan Ruirui Ma Bing Li Zhihui Yuan Wenxuan Huang Yueying Wu Quan Hu Chunjing Lin Xingqi Zhao Bao Peng Limei Zhao Chunbao Zhang Lianjun Sun | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 7 | Meat quality and cooking attributes of thawed pork with different low field NMR T21显示文摘 | LI Chunbao LIU Dengyong ZHOU Guanghong | 2011 | Meat Science2011，92，2： | 1 |
| 8 | Stress-Blended Eddy Simulation of the Turbulent Flow Around Circular Cylinder with Dynamic Hybrid RANS/LES Model显示文摘In order to verify the effectiveness and superiority of the dynamic hybrid RANS/LES(DHRL)model,the flow around a cylinder with sinusoidal fluctuating velocity at the inlet was used as the test case.The latest computational fluid dynamics(CFD)model can flexibly choose any existing large-eddy simulation(LES)method combined with RANS method to calculate the flow field.In addition,the DLES model and DDES model are selected as typical representatives of the turbulence model to compare the capture ability of the flow field mechanism.The internal flow field including the y+value,velocity distribution,turbulent kinetic energy and vortex structures is comprehensively analyzed.Finally,the results show that the new model has enough sensitivity to capture the information of the flow field and has more consistent velocity distribution with the experimental value,which shows its potential in practical engineering applications to some extent. | Chunbao Liu Jing Li Weiyang Bu Dong Xu Wen Wu | 2019 | Journal of Beijing Institute of Technology2019，28，2： | 1 |
| 9 | Changes of meat quality characteristics and intramuscular connective tissue of beef semitendinosus muscle during postmortem aging for Chinese Yellow bulls显示文摘 | LI Chunbao ZHOU Guanghong XU Xinglian | 2008 | International Journal of Food Science and Technology2008，43，： | 1 |
| 10 | Changes of meat qualitycharacteristics and intramuscular connective tissue of beef semitendino-sus muscle during postmortem aging for Chinese Yellow bulls显示文摘 | LI Chunbao ZHOU Guanghong XU Xinglian | 2008 | International Journal of Food Science and Technology2008，43，5： | 1 |
| 11 | Meat quality and cooking attributes of thawed pork with different low field NMR T2j 显示文摘 | Li Chunbao Liu Dengyong Zhou Guanghong | 2012 | Meat Science2012，92，2： | 1 |
| 12 | Comparisons of meat quality characteristics and intramuscular connective tissue between beef longissimus dorsi and semitendinosus muscles from Chinese yellow bulls显示文摘 | LI Chunbao ZHOU Guanghong XU Xinglian | 2007 | Journal of Muscle Foods2007，18，2： | 1 |
| 13 | Changes in meat quality of ovine longissimus dorsi muscle in response to repeated freeze and thaw显示文摘 | QI Jun LI Chunbao CHEN Yinji | 2012 | Meat Science2012，92，4： | 1 |
| 14 | Meat quality and cooking attributes of thawed pork with different low field NMR T 21显示文摘 | Chunbao Li Dengyong Liu Guanghong Zhou Xinglian Xu Jun Qi Peilei Shi Tianlan Xia | 2011 | Meat Science2011，，2： | 1 |
| 15 | Changes in meat quality of ovine longissimusdorsi muscle in response to repeated freeze and thaw显示文摘 | QI Jun LI Chunbao CHEN Yinji | 2012 | Meat Science2012，92，4： | 1 |
| 16 | Effects of low-voltage electrical stimulation and rapid chilling on meat quality characteristics of Chinese crossbred bulls显示文摘 | LI Chunbao CHEN Yinji XU Xinglian | 2006 | Meat Science2006，72，1： | 1 |
| 17 | Changes of intramuscular phospholipids and free fatty acids during the processing of Nanjing dry-cured duck显示文摘 | Weimin Xu Xinglian Xu Guanghong Zhou Daoying Wang Chunbao Li | 2007 | Food Chemistry2007，，2： | 1 |
| 18 | Meat quality and cooking attributes of thawed pork with different low field NMR T21 显示文摘 | Li Chunbao Liu Dengyong Zhou Guanghong | 2012 | Meat Science2012，92，2： | 1 |
| 19 | Meat quality and cooking attributes of thawed pork with different low field NMR T21显示文摘 | Chunbao Li Dengyong Liu Guanghong Zhou | 2012 | Meat Science2012，92，： | 1 |
| 20 | Changes of meat quality characteristics and intramuscular connective tissue of beef Semitendino- sus muscle during postmortem aging for Chinese Yellow bulls显示文摘 | LI Chunbao ZHOU Guanghong XU Xinghan | 2008 | International Journal of Food Science and Technology2008，43，： | 1 |
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