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| 1 | Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect显示文摘Targeted genome modifications with the Cas9/gRNA system derived from the clustered regularly interspaced short palindromic repeat/CRISPR-associated(CRISPR/Cas)system have been successfully used in cultured human cells as well as in most model organisms,including zebrafish(Danio rerio),mouse,and fruit fly(Chang et al.,2013;Cong et al.,2013; | Da Liu Zhanxiang Wang An Xiao Yutian Zhang Wenyuan Li Yao Zu Shaohua Yao Shuo Lin Bo Zhang | 2014 | Journal of Genetics and Genomics2014，41，1： | 18 |
| 2 | Shen-Fu injection reduces impaired myocardial β-adrenergic receptor signaling after cardiopulmonary resuscitation显示文摘 | JI Xian-fei JI Hong-bin SANG De-ya WANG Shuo YANG Lin LI Chun-sheng | 2013 | Chinese Medical Journal2013，，4： | 16 |
| 3 | miR-221/222 activate the Wnt/β-catenin signaling to promote triple-negative breast cancer显示文摘 | Sanhong Liu Zifeng Wang Zukai Liu Shuo Shi Zhaoran Zhang Jiawei Zhang Haifan Lin | 2018 | Journal of Molecular Cell Biology2018，10，4： | 16 |
| 4 | THE M/M/c QUEUE WITH PH SYNCHRONOUS VACATIONS显示文摘1.IntroductionRecently,singleserverqueueswithvacationshavebeenstudiedextensivelyfromtheirowntheoreticalinterestaswellastheirapplicationstomanyengineeringsystemssuchascomputers,communicationnetworksandmanufacturingsystems.FOrtheM/G/lqueueingsystemswit... | TIAN Nai shuo (Department of Mathematics, Yanshan University, Qinhuangdao 066004,China) LI Quan lin (National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100080, China) | 2000 | Systems Science and Mathematical Sciences2000，13，1： | 13 |
| 5 | Structural insights into a novel histone demethylase PHF8显示文摘histone methylation/demethylation 的动态规定在开发期间起一个重要作用。在人的植物 homeodomain (哲学博士) 的变化和截断摸蛋白质 8 (PHF8 ) 与 X 连接智力迟钝和面部异例被联系,例如一张长脸,宽广鼻音尖端,劈开 lip/cleft 腭和大手,然而,它的分子的功能和结构的基础仍然保持不清楚。这里,我们在高分辨率报导 PHF8 的催化核心的水晶结构与或没有伪 - ketoglutarate (伪 - KG ) 。生物化学、结构的研究表明 PHF8 是为甲醇化物 di 、单音甲醇化物的 histone H3 离氨酸 9,然而并非为 H3K9me3 特定的新奇 histone demethylase (H3K9me2/1 ) 。我们的分析也揭示人的 PHF8 怎么在 methylation 状态之间区别并且为 methylated H3K9 完成顺序特性。在里面 vitro demethylation 试金也证明在临床的病人观察的 F279S 异种不拥有 demethylation 活动,建议酶的活动的那损失为 PHF8 病人的致病是关键的。一起拿,这些结果将使位于联系 PHF8 的发展、神经病学的疾病下面的分子的机制清楚些。 | Lin Yu Yang wang Shuo Huang Jianjun Wang Zengqin Deng Qi Zhang Wei Wu Xingliang Zhang Zhao Liu Weimin Gong Zhongzhou Chen | 2010 | Cell Research2010，20，2： | 11 |
| 6 | Significance of Aspergillus spp. isolation from lower respiratory tract samples for the diagnosis and prognosis of invasive pulmonary aspergillosis in chronic obstructive pulmonary disease显示文摘 | HE Hang-yong CHANG Shuo DING Lin SUN Bing LI Fang ZHAN Qing-yuan | 2012 | Chinese Medical Journal2012，，17： | 10 |
| 7 | Charge Transport in Oil Impregnated Paper Insulation Under Temperature Gradient Using Transient Upstream FEM显示文摘Charge transport in oil impregnated paper impacts the insulation performance of a transformer.This paper proposes a simulation method for the charge transport in oil impregnated paper insulation.The transient upstream finite element method(FEM)is applied to the transport equations of bipolar charges for establishing a numerical simulation model of charge transport in oil impregnated paper insulation.The method is validated by experimental results.The charge transport and electric field distribution in single-layer oil impregnated paper insulation under different temperature gradients is simulated.The trends of the simulation results are seen to agree with the corresponding experimental results.This paper conducts exploratory research into the simulation of charge transportation phenomenon in oil impregnated paper,and is of importance to the design of oil impregnated paper insulation. | Shuo Jin Jiangjun Ruan Zhiye Du Zhifei Yang Taotao Zhou Guodong Huang Lin Zhu | 2015 | CSEE Journal of Power and Energy Systems2015，1，3： | 10 |
| 8 | Clinical utility of automated platelet clump count in the screening for ethylene diamine tetraacetic acid-dependent pseudothrombocytopenia显示文摘 | WU Wei GUO Ye ZHANG Lin CUI Wei LI Wei ZHANG Shuo | 2011 | Chinese Medical Journal2011，，20： | 9 |
| 9 | NMDA Receptor Antagonist MK801 Protects Against 1-Bromopropane-Induced Cognitive Dysfunction显示文摘Occupational exposure to 1-bromopropane(1-BP) induces learning and memory deficits. However, no therapeutic strategies are currently available. Accumulating evidence has suggested that N-methyl-D-aspartate receptors(NMDARs) and neuroinflammation are involved in the cognitive impairments in neurodegenerative diseases. In this study we aimed to investigate whether the noncompetitive NMDAR antagonist MK801 protects against 1-BPinduced cognitive dysfunction. Male Wistar rats were administered with MK801(0.1 mg/kg) prior to 1-BP intoxication(800 mg/kg). Their cognitive performance was evaluated by the Morris water maze test. The brains of rats were dissected for biochemical, neuropathological,and immunological analyses. We found that the spatial learning and memory were significantly impaired in the1-BP group, and this was associated with neurodegeneration in both the hippocampus(especially CA1 and CA3)and cortex. Besides, the protein levels of phosphorylated NMDARs were increased after 1-BP exposure. MK801 ameliorated the 1-BP-induced cognitive impairments and degeneration of neurons in the hippocampus and cortex.Mechanistically, MK801 abrogated the 1-BP-induced disruption of excitatory and inhibitory amino-acid balance and NMDAR abnormalities. Subsequently, MK801 inhibited the microglial activation and release of pro-inflammatory cytokines in 1-BP-treated rats. Our findings, for the first time, revealed that MK801 protected against 1-BP-induced cognitive dysfunction by ameliorating NMDAR function and blocking microglial activation, which might provide a potential target for the treatment of 1-BP poisoning. | Lin Xu Xiaofei Qiu Shuo Wang Qingshan Wang Xiu-Lan Zhao | 2019 | Neuroscience Bulletin2019，35，2： | 8 |
| 10 | Curcuminoids Target Decreasing Serum Adipocyte-fatty Acid Binding Protein Levels in Their Glucose-lowering Effect in Patients with Type 2 Diabetes显示文摘Whether supplementation of curcuminoids decreases serum adipocyte-fatty acid binding protein(A-FABP) level and whether this decrease benefits glucose control is unclear.One-hundred participants(n=50 administered curcuminoids,n=50 administered placebo) from our previous report on the effect of curcuminoids on type 2 diabetes in a 3-month intervention were assessed for levels of serum A-FABP,oxidative stress,and inflammatory biomarkers.Curcuminoids supplementation led to significant decreases in serum A-FABP,C-reactive protein(CRP),tumor necrosis factor-α,and interleukin-6 levels.Curcuminoids supplementation also significantly increased serum superoxide dismutase(SOD) activity.The change in serum A-FABP levels showed positive correlations with changes in levels of glucose,free fatty acids(FFAs),and CRP in subjects supplemented with curcuminoids.Further stepwise regression analysis showed that A-FABP was an independent predictor for levels of FFAs,SOD,and CRP.These results suggest that curcuminoids may exert anti-diabetic effects,at least in part,by reductions in serum A-FABP level.A-FABP reduction is associated with improved metabolic parameters in human type 2 diabetes. | NA Li Xin YAN Bo Lin JIANG Shuo CUI Hong Li LI Ying SUN Chang Hao | 2014 | Biomedical and Environmental Sciences2014，27，11： | 6 |
| 11 | Optimal power allocation for secure directional modulation networks with a full-duplex UAV user显示文摘This paper makes an investigation of a secure unmanned aerial vehicle(UAV)-aided communication network based on directional modulation(DM). In this network, ground base station(GBS) acts as a control center to transmit confidential message and artificial noise(AN). The UAV user, moving along a linear flight trajectory, is intended to receive the useful information from GBS. At the same time, it also sends AN signals to further interference eavesdropper’s channel. Aiming at maximizing secrecy rate during the UAV flight process, a joint optimization problem is formulated with respect to power allocation(PA)factors, beamforming vector and AN projection matrices. For simplicity, maximum rate transmission, nullspace projection and the leakage-based method are applied to form the transmit beamforming vector, AN projection matrix at GBS, and AN projection vector at UAV user, respectively. Following this, the optimization problem reduces to a bivariate optimization programme with two PA factors. An alternating iterative algorithm(AIA) is proposed to optimize the two PA factors. Simulation results demonstrate that, compared to the half-duplex(HD) mode, the proposed strategy for full-duplex(FD) mode achieves a higher secrecy rate(SR) and outperforms the FD mode with fixed PA strategy. | Zaoyu LU Linlin SUN Shuo ZHANG Xiaobo ZHOU Jinyong LIN Wenlong CAI Jin WANG Jinhui LU Feng SHU | 2019 | Science China(Information Sciences)2019，62，8： | 6 |
| 12 | Expansion of CRISPR/Cas9 genome targeting sites in zebrafish by Csy4-based RNA processing显示文摘 | Wei Qin Fang Liang Yan Feng Haipeng Bai Ruibin Yan Song Li Shuo Lin | 2015 | Cell Research2015，25，9： | 6 |
| 13 | Promoter methylation of Wnt/β-Catenin signal inhibitor TMEM88 is associated with unfavorable prognosis of nonsmall cell lung cancer显示文摘Objective:Recent research has indicated that altered promoter methylation of oncogenes and tumor suppressor genes is an important mechanism in lung cancer development and progression.In this study,we investigated the association between promoter methylation of TMEM88,a possible inhibitor of the Wnt/β-Catenin signaling,and the survival of patients with nonsmall cell lung cancer(NSCLC).Methods:Twelve pairs of tumor and adjacent non-tumor samples were used for microarray analyses of DNA methylation and gene expression.For validation,more than two hundred additional samples were analyzed for methylation using bisulfite pyrosequencing and for gene expression using q RT-PCR.Then the cell function were tested by wound healing,transwell,CCK8 and cell cycle assay.Results:Our analysis of patient specimens showed that TMEM88 methylation was higher in NSCLC tumors(82.2%±10.3,P<0.01)compared with the adjacent normal tissues(65.9%±7.2).The survival analysis revealed that patients with high TMEM88methylation had a shorter overall survival(46 months)compared with patients with low TMEM88 methylation(>56 months;P=0.021).In addition,we found that demethylation treatment could inhibit tumor cell proliferation,migration,and invasion,which was supportive of an association between methylation and survival.Conclusions:Based on these consistent observations,we concluded that TMEM88 may play an important role in NSCLC progression and that promoter methylation of TMEM88 may serve as a biomarker for NSCLC prognosis and treatment. | Rongna Ma Nannan Feng Xiao Yu Hongyan Lin Xiaohong Zhang Oumin Shi Huan Zhang Shuo Zhang Lei Li Min Zheng Ming Gao Herbert Yu Biyun Qian | 2017 | Cancer Biology & Medicine2017，14，4： | 6 |
| 14 | Reverse Genetic Approaches in Zebrafish显示文摘Zebrafish (Danio rerio ) 是一个生长得很好的脊椎动物动物模型。反向的遗传工具的一个全面集合为在这个有用有机体学习基因功能被开发了。Morpholino 是最广泛地使用的试剂 post-transcriptionally 将目标基因表示击倒。很长时间,指向了染色体修正重重地在大规模传统的向前基因的屏幕上被依靠了,例如 ENU (N-ethyl-N-nitrosourea ) mutagenesis 导出的 TILLING (指向在染色体导致了本地损害) 策略和伪 typed retrovirus 调停了 insertional mutagenesis。最近,设计了 endonucleases,包括 ZFN (锌手指核酸酶) 和 TALEN (抄写像使活跃之物的受动器核酸酶) ,提供新、有效的策略直接由在目标基因导致两倍海滨裂缝产生地点特定的 indel 变化。这里,我们为使用的 loss-of-function 研究总结主要反向的基因途径,包括基于为地点特定的基因指向的染色体宽的 mutagenesis 和方法的策略并且在 zebrafish 新兴。未来方向和期望将也被讨论。 | Peng Huang Zuoyan Zhu Shuo Lin Bo Zhang | 2012 | Journal of Genetics and Genomics2012，39，9： | 6 |
| 15 | Fatigue Crack Growth Rate of Ti-6Al-4V Considering the Effects of Fracture Toughness and Crack Closure显示文摘Fatigue fracture is one of the main failure modes of Ti-6A1-4V alloy,fracture toughness and crack closure have strong effects on the fatigue crack growth(FCG)rate of Ti-6A1-4V alloy.The FCG rate of Ti-6A1-4V is investigated by using experimental and analytical methods.The effects of stress ratio,crack closure and fracture toughness on the FCG rate are studied and discussed.A modified prediction model of the FCG rate is proposed,and the relationship between the fracture toughness and the stress intensity factor(SIF)range is redefined by introducing a correcting coefficient.Notched plate fatigue tests(including the fracture toughness test and the FCG rate test)are conducted to investigate the influence of affecting factors on the FCG rate.Comparisons between the predicted results of the proposed model,the Paris model,the Walker model,the Sadananda model,and the experimental data show that the proposed model gives the best agreement with the test data particularly in the near-threshold region and the Paris region,and the corresponding calculated fatigue life is also accurate in the same regions.By considering the effects of fracture toughness and crack closure,the novel FCG rate prediction model not only improves the estimating accuracy,but also extends the adaptability of the FCG rate prediction model in engineering. | ZHANG Junhong YANG Shuo LIN Jiewei | 2015 | Chinese Journal of Mechanical Engineering2015，28，2： | 5 |
| 16 | Tetramethylpyrazine prevents liver fibrotic injury in mice by targeting hepatocyte-derived and mitochondrial DNA-enriched extracellular vesicles显示文摘Liver fibrosis is the common consequence of almost all liver diseases and has become an urgent clinical problem without efficient therapies.Recent evidence has shown that hepatocytes-derived extracellular vesicles(EVs)play important roles in liver pathophysiology,but little is known about the role of damaged hepatocytes-derived EVs in hepatic stellate cell(HSC)activation and following fibrosis.Tetramethylpyrazine(TMP)from Ligusticum wallichii Franchat exhibits a broad spectrum of biological activities including liver protection.In this study,we investigated whether TMP exerted liver-protective action through regulating EV-dependent intercellular communication between hepatocytes and HSCs.Chronic liver injury was induced in mice by CCl_(4)(1.6 mg/kg,i.g.)twice a week for 8 weeks.In the last 4 weeks of CCl_(4) administration,mice were given TMP(40,80,160 mg·kg^(-1)·d^(-1),i.g.).Acute liver injury was induced in mice by injection of a single dose of CCl_(4)(0.8 mg/kg,i.p.).After injection,mice were treated with TMP(80 mg/kg)every 24 h.We showed that TMP treatment dramatically ameliorated CCl_(4)-induced oxidative stress and hepatic inflammation as well as acute or chronic liver fibrosis.In cultured mouse primary hepatocytes(MPHs),treatment with CCl_(4) or acetaminophen resulted in mitochondrial dysfunction,release of mitochondrial DNA(mtDNA)from injured hepatocytes to adjacent hepatocytes and HSCs through EVs,mediating hepatocyte damage and fibrogenic responses in activated HSCs;pretreatment of MPHs with TMP(25μM)prevented all these pathological effects.Transplanted serum EVs from TMP-treated mice prevented both initiation and progression of liver fibrosis caused by CCl_(4).Taken together,this study unravels the complex mechanisms underlying the protective effects of TMP against mtDNA-containing EV-mediated hepatocyte injury and HSC activation during liver injury,and provides critical evidence inspiring the development of TMP-based innovative therapeutic agents for the treatment of liver fibrosis. | Ya-jing Li Run-ping Liu Ming-ning Ding Qi Zheng Jian-zhi Wu Xiao-yong Xue Yi-qing Gu Bo-ning Ma Ya-jie Cai Shuo Li Sheng Lin Lu-yong Zhang Xiaojiaoyang Li | 2022 | Acta Pharmacologica Sinica2022，43，8： | 5 |
| 17 | Advances in tumor-endothelial cells co-culture and interaction on microfluidics显示文摘The metastasis in which the cancer cells degrade the extracellular matrix(ECM) and invade to the surrounding and far tissues of the body is the leading cause of mortality in cancer patients. With a lot of advancement in the field, yet the biological cause of metastasis are poorly understood. The microfluidic system provides advanced technology to reconstruct a variety of in vivo-like environment for studying the interactions between tumor cells(TCs) and endothelial cells(ECs). This review gives a brief account of both two-dimensional models and three-dimensional microfluidic systems for the analysis of TCs-ECs coculture as well as their applications to anti-cancer drug screening. Furthermore, the advanced methods for analyzing cell-to-cell interactions at single-cell level were also discussed. | Weiwei Li Mashooq Khan Sifeng Mao Shuo Feng Jin-Ming Lin | 2018 | Journal of Pharmaceutical Analysis2018，8，4： | 5 |
| 18 | High-conductivity free-standing Li_(6)PS_(5)Cl/poly(vinylidene difluoride)composite solid electrolyte membranes for lithium-ion batteries显示文摘Bulk-type all-solid-state batteries(ASSBs)using sulfide solid electrolyte are considered as a promising alternative to commercial lithium-ion batteries owing to their high energy density and safety.However,cell energy density is often comparatively low due to use of a thick solid electrolyte separator and a lithium alloy as anode.For achieving high-performance ASSBs,creating a thin free-standing electrolyte with high-conductivity and good cycling stability against lithium anode is essential.In this work,we present Li_(6)PS_(5)Cl/poly(vinylidene difluoride)(LPSCl/PVDF)composite electrolytes prepared by a slurry method.The influence of the PVDF content on the microstructure,morphology,ionic conductivity and activation energy of the LPSCl/PVDF electrolytes is systematically investigated.Free-standing LPSCl/PVDF membranes with a thickness of 100-120 mm and a high ionic conductivity of about 1·10^(-3) S cm^(-1) at 25℃ are obtained.After adding PVDF to the LPSCl electrolyte,the cycling stability of the LPSCl electrolyte against lithium metal improves significantly.Therefore,LPSCl/PVDF composite electrolytes are promising candidates to be used in ASSBs. | Shuo Wang Xue Zhang Sijie Liu Chengzhou Xin Chuanjiao Xue Felix Richter Liangliang Li Lizhen Fan Yuanhua Lin Yang Shen Jurgen Janek Ce-Wen Nan | 2020 | Journal of Materiomics2020，6，1： | 5 |
| 19 | Breast cancer stem-like cells can promote metastasis by activating platelets and down-regulating antitumor activity of natural killer cells显示文摘OBJECTIVE:To investigate whether cancer stem cells(CSCs) more efficiently activating platelets and evading immune surveillance than non-CSCs thus promoting metastasis.METHODS:We enriched and identified sphereforming cells(SFCs) and coincubated washed platelets with several platelet activators including collagen,4T1 and SFCs.Platelet-coating tumor cells,platelet activation and TGF-β1 release were analyzed.Then natural kell cells(NK) were incubated with supernatants of different activated platelet samples what we called sample release(SR).The degranulation assay and NKG2 D expression on NK cells were conducted by flow cytometry.Finally tissue factor(TF) expression of SFCs or 4T1 were evaluated by western blot.RESULTS:Breast cancer cell line 4T1 could form spheres in serum-free medium at low adherence.Sphere-forming cells expressed high levels of the CD24-/lowCD44 + stem cell phenotype.Both sphere-forming cells or 4T1 were coated with abundant platelets while sphere-forming cells induced significantly higher expression of platelet activating receptor CD62 p than 4T1 did(P < 0.01).And sphere-forming cells induced platelets to produce more TGF-β1 than 4T1 did(P < 0.01).Furthermore,sample releases induced by sphere-forming cells caused more vigorous inhibition of NK cells antitumor reactivity(P < 0.05) and reduced NKG2 D expression(P < 0.01).The final results showed that sphere-forming cells expressed higher levels of TF than 4T1(P < 0.05).CONCLUSION:Our findings indicate that CSCs could efficiently activate platelets,induce platelets to secrete more TGF-β1,decrease NKG2 D expression and inhibit antitumor activity of NK cell,compared with 4T1.And higher levels of TF expression of CSCs may account for this correlation of CSCs and platelets. | Wang Shuo Zhang Ying Cong Weihong Liu Jie Zhang Yuren Fan Huiting XuYonggang Lin Hongsheng | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 5 |
| 20 | Fabrication of virus-like particles with strip-pattern surface:A two-step self-assembly approach显示文摘Spherical nanostructures with striped patterns on the surfaces resembling the essential structures of natural virus particles were constructed through a two-step self-assembly approach of polystyrene-boligo(acrylic acid)(PS-b-oligo-AA) and poly(γ-benzyI L-glutamate)-b-poly(ethylene glycol)(PBLG-bPEG) copolymer mixtures in solution.On the basis of difference in hydrophilicity and self-assembly properties of the two copolymers,the two-step self-assembly process is realized.It was found that PS-boligo-AA copolymers formed spherical aggregates by adding a certain amount of water into polymer solutions in the first step.In the second step,two polymer solutions were mixed and water was further added,inducing the self-assembly of PBLG-b-PEG on the surfaces of PS-b-oligo-AA spheres to form striped patterns.In-depth study was conducted for the indispensable defects of striped patterns which are dislocations and +1/2 disclinations.The influencing factors such as the mixing ratio of two copolymers and the added water content in the first step on the morphology and defects of the striped patterns were investigated.This work not only presents an idea to interpret mechanism of the cooperative self-assembly behavior,but also provides an effective approach to construct virus-like particles and other complex structures with controllable morphology. | Shuo Zhang Chun-Hua Cai Zhou Guan Jia-Ping Lin Xing-Yu Zhu | 2017 | Chinese Chemical Letters2017，28，4： | 5 |
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