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| 1 | Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells显示文摘Clostridium butyricum has been widely considered an antibiotic substitute in recent years.It can promote growth performance,improve the immune response and enhance the intestinal barrier function of the host.In the present study,1-d-old Arbor Acres(AA)broilers were fed C.butyricum(1×109 cfu/kg)for 28 d.The transcriptomic characteristics of epithelial cells of the cecal mucosa were determined by RNA-sequence,and the cecal microbiota composition was explored by 16 S ribosomal RNA gene sequencing.The changes in the intestinal mucosa of broilers were then analyzed by tissue staining.Gene Ontology(GO)annotations identified substance transport and processes and pathways that might participate in intestinal development and cell viability.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that the differentially expressed genes are involved in numerous pathways related to amino acid and vitamin metabolism and antioxidant and defensive functions,among others.The relative expression of some genes associated with intestinal barrier function(claudins 2,15,19,and 23,tight junction proteins 1,2,and 3 and mucin 1)was significantly increased in the treatment group(P<0.05 or P<0.01).Moreover,the proportion of Firmicutes was higher in the C.butyricum-treated group,whereas the proportion of Proteobacteria was higher in the control group.At the genus level,the relative abundances of Butyricicoccus and Lactobacillus,among other bacteria,were increased after C.butyricum supplementation.The tissue staining analysis showed that the cecal mucosa of broilers was significantly ameliorated after the addition of C.butyricum(P<0.05 or P<0.01).These results showed that dietary supplementation with C.butyricum can enhance the antioxidant capacity,mucosal barrier function,and stabilize the cecal microbiota,resulting in improving the growth performance. | Laipeng Xu Xiangli Sun Xianhua Wan Keke Li Fuchun Jian Wenting Li Ruirui Jiang Ruili Han Hong Li Xiangtao Kang Yanbin Wang | 2021 | Animal Nutrition2021，，4： | 12 |
| 2 | Superplastic Solid-Phase Welding of 40 Cr-T10A Steel显示文摘The microstructure of 40Cr and T10A steel sample and its surface to be welded is ultra-fined through salt-bath cyclic quenching and high frequency hardening, then the surface is cleaned. Under non-vacuum and no shielded gas, the welding parameter of isothermal superplastic solidphase welding and the effect of surface microstructure prior to pressure welding on the quality of joint are studied. At the temperature of 730~750°C and at initial strain rate of (2~4) × 10-4 s-1,the strength of the joint is up to or close to that of 40Cr base metal in 3~5 min pressure welding. | Keke ZHANG Yunlin YANG Changsheng WANG Hongke LI Jin XUE | 2001 | Journal of Materials Science & Technology2001，17，1： | 10 |
| 3 | Modifi cation of wheat bran insoluble and soluble dietary fibers with snail enzyme显示文摘Insoluble dietary fiber(IDF)and soluble dietary fiber(SDF)extracted from wheat bran were modifi ed by snail enzyme and their physicochemical properties(water retention capacity and oil retention capacity),functional properties(cholesterol adsorption capacity,glucose adsorption capacity and antioxidant activity)and structural characterizations were evaluated.The results showed that snail enzyme modification led to the significant increase in oil retention capacity of IDF,glucose adsorption capacity and cholesterol adsorption capacity of IDF and SDF.Enzymatic modification also markedly improved the DPPH radical scavenging capacity and reducing power of IDF and SDF.Meanwhile,scanning electron microscopy(SEM)analysis indicated the microstructures of IDF and SDF powders were signifi cantly changed.Fourier transfer-infrared spectrometry(FT-IR)showed that snail enzyme modifi cation could degrade the part of cellulose and hemicellulose of IDF and SDF.All these improved physicochemical and functional properties of IDF and SDF might depend on their structural changes.It suggested that snail enzyme modifi cation could effectively improve physicochemical and functional properties of IDF and SDF from wheat bran. | Xin Liu Keke Suo Pei Wang Xue Li Limin Hao Jiaqing Zhu Juanjuan Yi Qiaozhen Kang Jinyong Huang Jike Lu | 2021 | Food Science and Human Wellness2021，10，3： | 10 |
| 4 | Large-scale synthesis of single-source, thermally stable, and dual-emissive Mn-doped Zn-Cu-ln-S nanocrystals for bright white light-emitting diodes显示文摘 | Lucheng Peng Dongze Li Zhuolei Zhang Keke Huang Ying Zhang Zhan Shi Renguo Xie Wensheng Yang | 2015 | Nano Research2015，8，10： | 7 |
| 5 | Hypoxia-stressed cardiomyocytes promote early cardiac differentiation of cardiac stem cells through HIF-1α/Jagged1/Notch1 signaling显示文摘Hypoxia is beneficial for the differentiation of stem cells transplanted for myocardial injury,but mechanisms underlying this benefit remain unsolved. Here, we report the impact of hypoxia-induced Jagged1 expression in cardiomyocytes(CMs) for driving the differentiation of cardiac stem cells(CSCs).Forced hypoxia-inducible factor 1α(HIF-1α) expression and physical hypoxia(5% O_2) treatment could induce Jagged1 expression in neonatal rat CMs. Pharmacological inhibition of HIF-1α by YC-1 attenuated hypoxia-promoted Jagged1 expression in CMs. An ERK inhibitor(PD98059), but not inhibitors of JNK(SP600125), Notch(DAPT), NF-κB(PTDC), JAK(AG490), or STAT3(Stattic) suppressed hypoxiainduced Jagged1 protein expression in CMs. c-Kit^+ CSCs isolated from neonatal rat hearts using a magnetic-activated cell sorting method expressed GATA4, SM22α or vWF, but not Nkx2.5 and cTnI.Moreover, 87.3% of freshly isolated CSCs displayed Notch1 receptor expression. Direct co-culture of CMs with BrdU-labeled CSCs enhanced CSCs differentiation, as evidenced by an increased number of BrdU^+/Nkx2.5^+ cells, while intermittent hypoxia for 21 days promoted co-culture-triggered differentiation of CSCs into CM-like cells. Notably, YC-1 and DAPT attenuated hypoxia-induced differentiation.Our results suggest that hypoxia induces Jagged1 expression in CMs primarily through ERK signaling,and facilitates early cardiac lineage differentiation of CSCs in CM/CSC co-cultures via HIF-1α/Jagged1/Notch signaling. | Keke Wang Ranran Ding Yanping Ha Yanan Jia Xiaomin Liao Sisi Wang Rujia Li Zhihua Shen Hui Xiong Junli Guo Wei Jie | 2018 | Acta Pharmaceutica Sinica B2018，8，5： | 7 |
| 6 | The receptor-like cytoplasmic kinase RIPK regulates broad-spectrum ROS signaling in multiple layers of plant immune system显示文摘Production of reactive oxygen species(ROS)via the activity of respiratory burst oxidase homologs(RBOHs)plays a vital role in multiple layers of the plant immune system,including pathogen-associated molecular pattern-triggered immunity(PTI),damage-associated molecular pattern-triggered immunity(DTI),effector-triggered immunity(ETI),and systemic acquired resistance(SAR).It is generally established that RBOHD is activated by different receptor-like cytoplasmic kinases(RLCKs)in response to various immune elicitors.In this study,we showed that RPM1-INDUCED PROTEIN KINASE(RIPK),an RLCK VII subfamily member,contributes to ROS production in multiple layers of plant immune system.The ripk mutants showed reduced ROS production in response to treatment with all examined immune elicitors that trigger PTI,DTI,ETI,and SAR.We found that RIPK can directly phosphorylate the N-terminal region of RBOHD in vitro,and the levels of phosphorylated S343/S347 residues of RBOHD are sigfniciantly lower in ripk mutants compared with the wild type upon treatment with all tested immune elicitors.We further demonstrated that phosphorylation of RIPK is required for its function in regulating RBOHD-mediated ROS production.Similar to rbohd,ripk mutants showed reduced stomatal closure and impaired SAR,and were susceptible to the necrotrophic bacterium Pectobacterium carotovorum.Collectively,our results indicate that RIPK regulates broad-spectrum RBOHD-mediated ROS signaling during PTI,DTI,ETI,and SAR,leading to subsequent RBOHD-dependent immune responses. | Ping Li Lulu Zhao Fan Qi Nang Myint Phyu Sin Htwe Qiuying Li Dawei Zhang Fucheng Lin Keke Shang-Guan Yan Liang | 2021 | Molecular Plant2021，14，10： | 5 |
| 7 | Predictive cruise control for heavy trucks based on slope information under cloud control system显示文摘With the advantage of fast calculation and map resources on cloud control system(CCS), cloud-based predictive cruise control(CPCC) for heavy trucks has great potential to improve energy efficiency, which is significant to achieve the goal of national carbon neutrality. However, most investigations focus on the on-board predictive cruise control(PCC) system,lack of research on CPCC architecture under CCS. Besides, the current PCC algorithms have the problems of a single control target and high computational complexity, which hinders the improvement of the control effect. In this paper, a layered architecture based on CCS is proposed to effectively address the realtime computing of CPCC system and the deployment of its algorithm on vehicle-cloud. In addition, based on the dynamic programming principle and the proposed road point segmentation method(RPSM), a PCC algorithm is designed to optimize the speed and gear of heavy trucks with slope information. Simulation results show that the CPCC system can adaptively control vehicle driving through the slope prediction, with fuel-saving rate of 6.17% in comparison with the constant cruise control. Also,compared with other similar algorithms, the PCC algorithm can make the engine operate more in the efficient zone by cooperatively optimizing the gear and speed. Moreover, the RPSM algorithm can reconfigure the road in advance, with a 91% roadpoint reduction rate, significantly reducing algorithm complexity.Therefore, this study has essential research significance for the economic driving of heavy trucks and the promotion of the CPCC system. | LI Shuyan WAN Keke GAO Bolin LI Rui WANG Yue LI Keqiang | 2022 | Journal of Systems Engineering and Electronics2022，33，4： | 5 |
| 8 | Primary resistance to crizotinib treatment in a non-small cell lung cancer patient with an EML4-ALK rearrangement:a case report显示文摘Crizotinib,a small molecular tyrosine kinase inhibitor,manifests dramatic responses in patients with non-small cell lung cancer with echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase(EML4-ALK)rearrangements.ALK gene point mutation is the primary mechanism of acquired crizotinib resistance;however,the intrinsic mechanism is not fully understood.Here,we report a patient with a low mutant allele fraction(MAF)of EML4-ALK rearrangement,who experienced primary resistance to crizotinib treatment.The patient was a 66-year-old Chinese man,who had a history of metastatic lung cancer and was treated with first-and third-generation epidermal growth factor receptor tyrosine kinase inhibitors(EGFR TKIs).After 14 months of osimertinib treatment,his disease progressed,and next-generation sequencing was performed from a liquid biopsy of the patient’s blood.An EML4-ALK rearrangement was found and crizotinib was administered.The patient’s lung lesions continued to progress after one month of crizotinib treatment,and pemetrexed-bevacizumab was initiated.After two cycles of chemotherapy,the metastatic cancers shrunk,and the patient maintained stable disease at his last follow-up.EML4-ALK rearrangements can happen in patients with EGFR-positive NSCLC,after acquired resistance to EGFR TKI treatment.The EGFR T790M and C797G mutations occur in cis is a critical mechanism of resistance to osimertinib therapy.The MAF of EML4-ALK rearrangements in cancer cells might be a predictive factor for crizotinib treatment. | Ling Zhang Yunxia Li Shaohong Zhang Chen Gao Keke Nie Youxin Ji | 2018 | Cancer Biology & Medicine2018，15，2： | 5 |
| 9 | Effects of different introduction methods of Ce^4+and Zr^4+on denitration performance and anti-K poisoning performance of V2O5-WO3/TiO2 catalyst显示文摘The purpose of this work is to explore the effects of the introduction methods of Ce^4+and Zr^4+on the physicochemical properties,activity,and K tolerance of V2 O5-WO3/TiO2 catalyst for the selective catalytic reduction of NOx by NH3.Four different methods,namely pre-impregnation,post-impregnation,coimpregnation,and co-precipitation,were used to synthesize a series of V2 O5-WO3-TiO2-CeO2-ZrO2 catalysts.The catalysts were characterized by XRD,BET,NH3-TPD,XPS,and H2-TPR techniques.Moreover,the activity and anti-K poisoning performance were tested by an NH3-SCR model reaction.The results show that the introduction of Ce^4+and Zr^4+can improve the catalytic performance of V2O5-WO3/TiO2 catalyst,but the impregnation method cannot enhance the anti-K poisoning performance.Ce^4+and Zr^4+introduced by co-precipitation method can effectively improve the tolerance of K,which is mainly due to the incorporation of Ce^4+and Zr^4+into TiO2 lattice to form a uniform TiO2-CeO2-ZrO2 solid solution,resulting in the optimal surface acidity and redox performance,and reducing the decreases caused by Kpoisoning.Furthermore,based on the best introduction method,we further optimized the molar ratio of Ce^4+/Zr^4+,It is found that the catalyst exhibits the best anti-K poisoning performance when the molar ratio of Ce^4+/Zr^4+is 2:1. | Jun Cao Xiaojiang Yao Li Chen Keke Kang Min Fu Yang Chen | 2020 | Journal of Rare Earths2020，38，11： | 4 |
| 10 | Carbon isotope composition and comparison of Lower Triassic marine carbonate rocks from Southern Longmenxia section in Guang'an, Sichuan Basin显示文摘The Early Triassic was a period of ecological restoration for the earth's system after the end-Permian mass extinction.Geochemical records linked to the variations in marine ecosystems during this period have attracted the interest of geologists for many years.Based on petrographic analysis of samples and evaluations of their reliability as proxies for original seawater,this study investigated the carbon and oxygen isotopic compositions of 350 carbonate rock samples from the Lower Triassic(and adjacent strata)in the southern Longmenxia section of Guang'an,eastern Sichuan Basin.The results indicate that the Triassic carbonate rocks from the southern Longmenxia section favorably preserved the original seawater'sδ^(13)C signal.Furthermore,carbon and oxygen isotopic compositions are found to be poorly correlated,with a determination coefficient as low as 0.0205 and only 44 rock samples show results of Mn/Sr>2 and/orδ^(18)0<-6.5‰.The complete carbon isotopic curve for the Lower Triassic is established using the data from the remaining 306 samples with Mn/Sr<2 and/orδ^(18)O>-6.5‰.This curve presents favorable comparability on a global scale,specifically in theδ^(13)C minima near the Permian-Triassic boundary,at the top of the Jia1 and within the the Jia3,as well as in theδ^(13)C maxima at the tops of the Ye1 and Ye4,at the base of the Jia2 and at the top of the Ye3.The peaks and troughs corresponding to these maxima and minima all reflect global signals.By comparing these results to previous research on coeval carbon isotopic curves established within the chronostratigraphic framework,the ages of these critical evolution points are determined.The results show that the Ye1 roughly corresponds to the Griesbachian substage;the Ye2 through Ye4 correspond to the Dienerian substage;Jial corresponds to the Smithian substage;from the Jia2 to the lower part of the Jia4 correspond to the Spathian substage;and the middle and upper parts of the Jia4 belongs to the Aegean Substage of the Middle Triassic.Around the boundary between the Jial and Jia2(which represents the Smithian-Spathian boundary(SSB)),the value ofδ^(13)C increases rapidly from-0.911‰to 3.679‰.The span during which the seawater's carbon isotope experiences this drastic change may be less than36 kyr.The oxygen isotope,which is more sensitive to sedimentary environmental changes,exhibits changes prior to the carbon isotope near the SSB,indicating a significant increase in the salinity of the seawater before a sharp rise in the carbon isotope;this event leads to the formation of evaporites and dolomites. | HUANG SiJing HUANG KeKe ZHONG YiJiang LI XiaoNing MAO XiaoDong HU ZuoWei LIU SiBing ZHANG Meng WU WenHui | 2017 | Science China Earth Sciences2017，60，1： | 4 |
| 11 | 27-Hydroxycholesterol-induced EndMT acts via STAT3 signaling to promote breast cancer cell migration by altering the tumor microenvironment显示文摘Objective:The endothelial to mesenchymal transition(EndMT)plays a major role in cancer metastasis by regulating the complexity of the tumor microenvironment(TME).Here,we investigated whether 27-hydroxycholesterol(27 HC)induces EndMT in endothelial cells(ECs).Methods:EndMT markers in the human microvascular endothelial cell-1(HMEC-1)cell line and human umbilical vein endothelial cells(HUVECs)stimulated with 27 HC were evaluated with Western blot.Epithelial to mesenchymal transition(EMT)markers in breast cancer(BC)cells cultured in conditioned medium were investigated with quantitative real time polymerase chain reaction(qRT-PCR).The MMP-2 and MMP-9 mRNA expression and activity were detected with qRT-PCR and gelatin zymography assays,respectively.The effect of activated STAT3 on 27 HC-induced EndMT was validated by Western blot,immunofluorescence staining,and cell transfection assays.The migration ability of BC cells was evaluated with Transwell assays.Results:We found that 27 HC induced EndMT in HMEC-1 and HUVECs,and 27 HC-induced EndMT facilitated EMT and BC cell migration.The 27 HC-induced EMT of BC cells also promoted EndMT and HUVEC migration.Investigation of the underlying molecular mechanisms revealed that STAT3 knockdown repressed EndMT in HUVECs as well as migration in BC cells induced with 27 HC.In addition,C646 and resveratrol,inhibitors of STAT3 acetylation,repressed the expression of Ac-STAT3,p-STAT3,and EndMT markers in HUVECs exposed to 27 HC;these HUVECs in turn attenuated the migration ability of BC cells in 27 HC-induced EndMT.Conclusions:Cross-talk between 27 HC-induced EndMT and EMT was observed in the TME.Moreover,activation of STAT3 signaling was found to be involved in 27 HC-induced EndMT. | Kailin Jiao Jing Zhen Maoxuan Wu Mengying Teng Keke Yang Qian Zhou Chunyan Hu Ming Zhou Yuan Li Zhong Li | 2020 | Cancer Biology & Medicine2020，17，1： | 3 |
| 12 | Proteasome Inhibitor PS-341 Effectively Blocks Infection by the Severe Fever with Thrombocytopenia Syndrome Virus显示文摘Severe fever with thrombocytopenia syndrome(SFTS)is an emerging hemorrhagic fever disease caused by SFTSV,a newly discovered phlebovirus that is named after the disease.Currently,no effective vaccines or drugs are available for use against SFTSV infection,as our understanding of the viral pathogenesis is limited.Bortezomib(PS-341),a dipeptideboronic acid analog,is the first clinically approved proteasome inhibitor for use in humans.In this study,the antiviral efficacy of PS-341 against SFTSV infection was tested in human embryonic kidney HEK293T(293T)cells.We employed four different assays to analyze the antiviral ability of PS-341 and determined that PS-341 inhibited the proliferation of SFTSV in 293T cells under various treatment conditions.Although PS-341 did not affect the virus absorption,PS-341 treatment within a non-toxic concentration range resulted in a significant reduction of progeny viral titers in infected cells.Dual-luciferase reporter assays and Western blot analysis revealed that PS-341 could reverse the SFTSV-encoded nonstructural protein(NS)mediated degradation of retinoic acid-inducible gene-1(RIG-I),thereby antagonizing the inhibitory effect of NSs on interferons and blocking virus replication.In addition,we observed that inhibition of apoptosis promotes virus replication.These results indicate that targeting of cellular interferon pathways and apoptosis during acute infection might serve as the bases of future therapeutics for the treatment of SFTSV infections. | Sihua Liu Hongyun Liu Keke Zhang Xueping Li Yuqin Duan Zhiyun Wang Tao Wang | 2019 | Virologica Sinica2019，34，5： | 3 |
| 13 | Efficient Solution to Secure ECC Against Side-channel Attacks显示文摘 | WU Keke LI Huiyun ZHU Dingju YU Fengqi | 2011 | Chinese Journal of Electronics2011，20，3： | 3 |
| 14 | OsCYCP4s coordinate phosphate starvation signaling with cell cycle progression in rice显示文摘Phosphate starvation leads to a strong reduction in shoot growth and yield in crops.The reduced shoot growth is caused by extensive gene expression reprogram-ming triggered by phosphate deficiency,which is not itself a direct consequence of low levels of shoot phosphorus.However,how phosphate starvation inhibits shoot growth in rice is still unclear.In this study,we determined the role of OsCYCP4s in the regulation of shoot growth in response to phosphate starvation in rice.We demonstrate that the ex-pression levels of OsCYCP4s,except OsCYCP4;3,were induced by phosphate starvation.Overexpression of the phosphate starvation induced OsCYCP4s could compete with the other cyclins for the binding with cyclin-dependent kinases,therefore suppressing growth by reducing cell proliferation.The phosphate starvation induced growth inhibition in the loss-of-function mutants cycp4;1,cycp4;2,and cycp4;4 is par-tially compromised.Furthermore,the expression of some phosphate starvation inducible genes is negatively modu-lated by these cyclins,which indicates that these OsCYCP4s may also be involved in phosphate starvation signaling.We conclude that phosphate starvation induced OsCYCP4s might coordinate phosphate starvation signaling and cell cycle progression under phosphate starvation stress. | Lei Xu Fang Wang Ruili Li Minjuan Deng Meilan Fu Huiying Teng Keke Yi | 2020 | Journal of Integrative Plant Biology2020，62，7： | 2 |
| 15 | Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle显示文摘Background:Systemic lupus erythematosus(SLE)is a complex autoimmune disease,and the mechanism of SLE is yet to be fully elucidated.The aim of this study was to explore the role of two-pore segment channel 2(TPCN2)in SLE pathogenesis.Methods:Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression ofTPCN2 in SLE.We performed a loss-of-function assay by lentiviral construct in Jurkat and THP-1 cell.Knockdown ofTPCN2 were confirmed at the RNA level by qRT-PCR and protein level by Western blotting.Cell Count Kit-8 and flow cytometry were used to analyze the cell proliferation,apoptosis,and cell cycle ofTPCN2-deficient cells.In addition,gene expression profile ofTPCN2-deficient cells was analyzed by RNA sequencing(RNA-seq).Results:TPCN2 knockdown with short hairpin RNA(shRNA)-mediated lentiviruses inhibited cell proliferation,and induced apoptosis and cell-cycle arrest of G2/M phase in both Jurkat and THP-1 cells.We analyzed the transcriptome of knockdown-TPCN2-Jurkat cells,and screened the differential genes,which were enriched for the G2/M checkpoint,complement,and interleukin-6-Janus kinase-signal transducer and activator of transcription pathways,as well as changes in levels of forkhead box O,phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin,and T cell receptor pathways;moreover,TPCN2 significantly influenced cellular processes and biological regulation.Conclusion:TPCN2 might be a potential protective factor against SLE. | Keke Li Jingkai Xu Ke Xue Ruixing Yu Chengxu Li Wenmin Fei Xiaoli Ning Yang Han Ziyi Wang Jun Shu Yong Cui | 2022 | Chinese Medical Journal2022，，4： | 2 |
| 16 | Synthesis of sulfur doped C_(3)N_(4) with enhanced photocatalytic activity in molten salt显示文摘In this paper,sulfur doped g-C_(3)N_(4)(S-g-C_(3)N_(4))was successfully prepared at 500℃ for 3 h via a modified molten salt method using dicyandiamide as the main raw material,trithiocyanuric acid as the sulfur source and LiBreKCl as the reaction medium.The as-prepared SeCN5.0% sample(the mass ratio of trithiocyanuric acid to dicyandiamide was 5.0%)composed of irregular flakes showed a band gap of 1.83 eV,which was narrower than that(2.55 eV)of pristine g-C_(3)N_(4).The SeCN5.0% sample also exhibited an outstanding absorption capacity of visible light.Moreover,the photodegradation rate toward methylene blue and tetracycline were respectively 10 and 20 times as high as that of bulk g-C_(3)N_(4) prepared by conventional heating methods,confirming its superior photocatalytic performance.These results can be attributed to that the replacement of lattice nitrogen with sulfur atom tuned the electronic structure of g-C_(3)N_(4),improved the absorption of visible light,optimized the separation of photogenerated electron-hole pairs,and consequently enhanced the photocatalytic activity of g-C_(3)N_(4).Moreover,the trapping experiments implied that hole(ht)and superoxide radical($O2)were the main active species in the process of photodegradation. | Keke Guan Junyi Li Wen Lei Honghong Wang Zhaoming Tong Quanli Jia Haijun Zhang Shaowei Zhang | 2021 | Journal of Materiomics2021，7，5： | 2 |
| 17 | Injectable silk/hydroxyapatite nanocomposite hydrogels with vascularization capacity for bone regeneration显示文摘Localized and sustained osteogenic-angiogenic stimulation to bone defects is critical for effective bone repair.Here,desferrioxamine(DFO)was loaded on silk fibroin nanofibers and blended with hydroxyapatite nanorods(HA),forming injectable DFO-loaded silk fibroin-HA nanocomposite hydrogels.The composite hydrogels remained homogeneous distribution of HA with high ratio(60%)and also higher stiffness than that of pure silk fibroin nanofiber hydrogels,which provided stable osteogenic stimulation niches for tissue regeneration.Without the scarify of injectability,the hydrogels achieved slow delivery of DFO for above 60 days,resulting in suitable angiogenesis in vitro and in vivo and better osteogenesis than DFO-free systems.Compared to previous injectable silk fibroin-HA hydrogels,the introduction of vascularization capacity further stimulated the osteogenic differentiation of stem cells and accelerated new bone formation.Quicker and better bone healing were detected at defect sites after the injection of DFO-loaded nanocomposite hydrogels,indicating the effective synergistic effect of osteogenic and angiogenic cues.This work provides a simple and effective strategy of introducing angiogenic cues to bone matrices.We believe that the injectable nanocomposite hydrogels are suitable for the regeneration of bone tissues. | Keke Wang Weinan Cheng Zhaozhao Ding Gang Xu Xin Zheng Meirong Li Guozhong Lu Qiang Lu | 2021 | Journal of Materials Science & Technology2021，，4： | 2 |
| 18 | Strategies for improving fertilizer phosphorus use efficiency in Chinese cropping systems显示文摘A four-year project,entitled'The mechanisms of fraction transformation and high use efficiency of P fertilizer in Chinese cropping systems'commenced in 2017.The project was established to answer three key questions and looked at 17 cropping systems on ten soils.First,we asked what are the dynamics of transformation,fixation and mobilization of P fertilizers in soil-cropping systems?Second,what are the mechanisms of soil-cropmicrobe interactions by which P fertilizer can be efficiently used?Third,how to manipulate the processes of P use in cropping systems?The targets of this project are(1)to explore the mechanisms of P fixation,the pathways of loss of P availability and the threshold of migration of fertilizer P in the field;(2)to uncover mechanisms by which soil legacy P is mobilized through root physiological and morphological processes and through arbuscular mycorrhizal fungi and P-solubilizing bacteria in rhizosphere and hyphosphere;(3)to estimate the biological potential of crops for high efficiency P absorption and use;(4)to innovate new approaches for improving the efficiency of P fertilizers.The outcomes will provide theoretical support for setting standards for limitation of P fertilizer application rate in the main cropping zones of China. | Gu FENG Jingping GAI Xionghan FENG Haigang LI Lin ZHANG Keke YI Jialong LV Yiyong ZHU Li TANG Yilin LI | 2019 | Frontiers of Agricultural Science and Engineering2019，6，4： | 2 |
| 19 | Working-in-tandem mechanism of multi-dopants in enhancing electrocatalytic nitrogen reduction reaction performance of carbon- based materials显示文摘Developing carbon-based electrocatalysts with excellent N2 adsorption and activation capability holds the key to achieve highly efficient nitrogen reduction reaction(NRR)for reaching its practical application.Here,we report a highly active electrocatalyst--metal-free pyrrolic-N dominated N,S co-doped carbon(pyrr-NSC)for NRR.Based on theoretical and experimental results,it is confirmed that the N and S-dopants practice a working-in-tandem mechanism on pyrr-NSC,where the N-dopants are utilized to create electropositive C sites for enhancing N2 adsorption and the S-dopants are employed to induce electron backdonation for facilitating N2 activation.The synergistic effect of the pyrrolic-N and S-dopants can also suppress the irritating hydrogen evolution reaction,further boosting the NRR performance.This work gives an indication that the combination of two different dopants on electrocatalyst can enhance NRR performance by working in the two tandem steps-the adsorption and activation of N2 molecules,providing a new strategy for NRR electrocatalyst design. | Wenqing Zhang Keke Mao Jingxiang Low Hengjie Liu Yanan Bo Jun Ma Qiaoxi Liu Yawen Jiang Jiuzhong Yang Yang Pan Zeming Qi Ran Long Li Song Yujie Xiong | 2021 | Nano Research2021，14，9： | 2 |
| 20 | Saddle-Dolomite-Bearing Fracture Fillings and Records of Hot Brine Activity in the Jialingjiang Formation,Libixia Section,Hechuan Area of Chongqing City显示文摘Most vein minerals deposited in fractures of the Jialingjiang Formation from Libixia section,Hechan area include a large amount of saddle dolomite and accompanying celestite,calcite and fluorite.This study analyzed the nature,source,evolution of the fluids by plane-light petrography,fluid-inclusion methods,cathodoluminescence images,and stable isotopic compositions.The homogenization temperatures of two-phase aqueous fluid inclusions in dolomite range between100 and 270℃.Combined with theδ^(18)O data,it is suggested that the fluid responsible for the precipitation of fracture fillings haveδ^(18)O values between 10‰and 18‰(relative to SMOW).The saddle dolomite and the accompanying minerals were the result of activity of dense brines at elevated temperatures.Moreover,analysis shows that the fluid was derived from a mixture of marine-derived brine and deeper circulating flow.This fluid was enriched in Sr during diagenesis and formed celestite in fracture and for regional mineralization.Dissolution of saddle dolomite was attributed to the cooling of Mg/Ca-decreased fluids,which may relate to a leaching of gypsum to celestite in surrounding carbonates. | HUANG Keke ZHONG Yijiang HUANG Sijing LI Xiaoning FENG Mingshi | 2016 | Acta Geologica Sinica(English Edition)2016，90，1： | 2 |
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