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| 1 | Codon 249 mutation in exon 7 of p53 gene in plasma DNA:maybe a new early diagnostic marker of hepatocellular carcinoma in Qidong risk area,China显示文摘AIM: One of the characteristics of hepatocellular carcinoma (HCC) in Qidong area is the selective mutation resulting in a serine substitution at codon 249 of the p53 gene (1,20),and it has been identified as a 'hotspot' mutation in heptocellular carcinomas occurring in populations exposed to aflatoxin and with high prevalence of hepatitis B virus carriers (2, 3, 9, 10, 16, 24). We evaluated in this paper whether this 'hotspot' mutation could be detected in cellfree DNA circulating in plasma of patients with hepatocellular carcinoma and cirrhosis in Qidong, China, and tried to illustrate the significance of the detection of this molecular biomarker.METHODS: We collected blood samples from 25hepatocellular carcinoma patients, 20 cirrhotic patients and 30 healthy controls in Qidong area. DNA was extracted and purified from 200 μl of plasma from each sample. The 249ser p53 mutation was detected by restriction digestion analysis and direct sequencing of exon-7 PCR products.RESULTS: We found in exon 7 of p53 gene G→T transversion at the third base of codon 249 resulting 249Arg→249ser mutation in 10/25 (40%) hepatocellular carcinoma cases,4/20 (20%) cirrhotics, and 2/30 (7 %) healthy controls.The adjusted odds ratio for having the mutation was 22.1(95 % CI, 3.2~91.7) for HCC cases compared to controls.CONCLUSION: These data show that the 249ser p53mutation in plasma is strongly associated with hepatocellular carcinoma in Qidong patients. We found this mutation was also detected, although it was at a much lower frequency,in plasma DNA of Qidong cirrhotics and healthy controls;We consider that these findings, together with the usual method of HCC diagnosis, will give more information in early diagnosis of HCC, and 249ser p53 mutation should be developed to a new early diagnostic marker for HCC. | Xing-HuaHuang Lu-HongSun Dong-DongLu YanSun Li-JieMa Xi-RanZhang JianHuang LongYu | 2003 | World Journal of Gastroenterology2003，9，4： | 18 |
| 2 | Hybrid PdAg alloy-Au nanorods: Controlled growth, optical properties and electrochemical catalysis显示文摘高质量的混合 nanostructures 的可控制的生长为层次、复杂、多功能的设备的制造是高度合乎需要的。这里, PdAg 合金在金 nanorods 上的不同地点是 controllably 成年的,生产有在金牌的结束的 PdAg 的像哑铃的 nanostructures 有种的 PdAg 的 nanorods 或分叉的 nanostructures 对金 nanorods 几乎垂直。金 nanorods 上的 PdAg 合金的成核地点能被改变 H2PdCl4, AgNO3 和 cetyltrimethylammonium 溴化物(CTAB ) 的集中有效地调节。像哑铃、分叉的 nanostructures 被传播描绘电子显微镜学(TEM ) ,高分辨率的 TEM (HRTEM ) ,扫描线的精力散的 X 光检查光谱学(版本) , X 光检查光电子光谱学(XPS ) 和紫外力的吸收光谱学。他们的 electrocatalytic 表演作为探查反应用乙醇氧化被评估。像哑铃的 nanostructures 比分叉的显示出更好毒害反的表演,而是一项更坏的电气化学的活动。结果为基本研究或潜在的应用为复杂 nanostructures 的控制生长提供指南。 | Qiao Zhang Xia Guo Zhenxing Liang Jianhuang Zeng Jian Yang Shijun Liao | 2013 | Nano Research2013，6，8： | 10 |
| 3 | Stress Effect of Cadmium Absorption between Aloe and Solanum nigrum L显示文摘The stress effect of cadmium absorption between aloe and Solanum nigrum L was studied through a simulation experiment with different cadmium contents in soil.The results showed that the growth characteristics,biomass and Cd content in the plants of aloe and S.nigrum L were closely related to Cd content in the soil,and S.nigrum L and aloe had a strong tolerance to Cd in the experiment.pH of rhizosphere soil with S.nigrum L and aloe planted was generally higher than that of non-rhizosphere soil,which is related to the planting pattern.Compared with aloe,S.nigrum L had a stronger capacity to accumulate Cd,and intercropping S.nigrum L and aloe could inhibit aloe's absorption of Cd,which is favorable to the safe planting of aloe.In addition,S.nigrum L has the potential to restore polluted soil. | Chen Yingzhi Fu Shanming Wang Jianhuang Pei Jie Li Wenhao He Caizhen | 2015 | Meteorological and Environmental Research2015，6，3： | 4 |
| 4 | An Adaptive PM Model Based on Difference Eigenvalue for Image Restoration显示文摘Classic Perona-Malik(PM) model is usually used to smooth noise for the degraded image. However, to our knowledge, a well-known defect of the PM model is prone to cause ‘staircase' effect and blur image fine feature because of the lack of correct diffusion strength in diffusion process. To tackle the problem, we will improve the PM model by developing a new structure descriptor to adjust anisotropic diffusion strength for smoothing noise and preserving image feature adaptively. The new structure descriptor is based on image local geometry-Hessian matrix, called difference eigenvalue, which can adaptively track edge feature and high degree of homogeneity in an image, even when the observed image is blurred. Experiments on both nature and medical images show that the improved PM method can achieve a superior performance than the traditional methods in terms of visual inspection and quantitative measurements. | TIAN Haiying CAI Hongmin LAI Jianhuang | 2014 | Chinese Journal of Electronics2014，23，4： | 3 |
| 5 | The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins显示文摘Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1M NaCl. When the salt concentration was slowly reduced to 650 mMand 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic 'beads-on-a-string' structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution ofnucleosome,the mono- and di- nucleosomes were assembled in vitro with both HS2core (-10681 to -10970 bp) and NCR2 (-372to -194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG 14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression. | SHUBINGZHANG JIANHUANG HUIZHAO YIZHANG CHUNHUIHOU XIAODONGCHENG CHUJIANG MINQIANLI JUNHU RUOLANQIAN | 2003 | Cell Research2003，13，5： | 2 |
| 6 | The soybean Dof‐type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plants显示文摘 | Hui‐WenWang BoZhang Yu‐JunHao JianHuang Ai‐GuoTian YongLiao | 2007 | The Plant Journal2007，，4： | 2 |
| 7 | Out- put-only modal identification of cable-stayed bridge using wireless monitoring system显示文摘 | Wen Jianhuang Loh Chinhsiung Linch J P | 2008 | En- gineering Structures2008，30，： | 1 |
| 8 | Combining Multiple Clusterings via Crowd AgreementEstimation and Multi-granularity Link Analysis 显示文摘 | HUANG Dong LAI Jianhuang WANG Changdong | 2015 | Neurocomputing2015，170，： | 1 |
| 9 | A virtual reality based surgical skills training simulator for catheter ablation with real-time and robust interaction显示文摘Background Atrial fibrillation(AF)is the most common sustained cardiac arrhythmia that can cause severe heart problems.Catheter ablation is one of the most ideal procedures for the treatment of AF.Physicians qualified to perform this procedure need to be highly skilled in manipulating the relevant surgical devices.This study proposes an interactive surgical simulator with high fidelity to facilitate efficient training and low-cost medical education.Methods We used a shared centerline model to simulate the interaction between multiple surgical devices.An improved adaptive deviation-feedback approach is proposed to accelerate the convergence of each iteration.The periodical beating of the human heart was also simulated in real time using the position-based dynamics(PBD)framework to achieve higher fidelity.We then present a novel method for handling the interaction between the devices and the beating heart mesh model.Experiments were conducted in a homemade simulator prototype to evaluate the robustness,performance,and flexibility of the proposed method.Preliminary evaluation of the simulator was performed by medical students,residents,and surgeons.Results The interaction between surgical devices,static vascular meshes,and beating heart mesh was stably simulated in a frame rate suitable for interaction.Conclusion Our simulator is capable of simulating the procedure of catheter ablation with high fidelity and provides immersive visual experiences and haptic feedback. | Haoyu WANG Jianhuang WU | 2021 | Virtual Reality & Intelligent Hardware2021，3，4： | 1 |
| 10 | Relationships between the Extratropical ENSO Precursor and Leading Modes of Atmospheric Variability in the Southern Hemisphere显示文摘Previous studies suggest that the atmospheric precursor of El Ni ?no–Southern Oscillation(ENSO) in the extratropical Southern Hemisphere(SH) might trigger a quadrapole sea surface temperature anomaly(SSTA) in the South Pacific and subsequently influence the following ENSO. Such a quadrapole SSTA is referred to as the South Pacific quadrapole(SPQ).The present study investigated the relationships between the atmospheric precursor signal of ENSO and leading modes of atmospheric variability in the extratropical SH [including the SH annular mode(SAM), the first Pacific–South America(PSA1) mode, and the second Pacific–South America(PSA2) mode]. The results showed that the atmospheric precursor signal in the extratropical SH basically exhibits a barotropic wavenumber-3 structure over the South Pacific and is significantly correlated with the SAM and the PSA2 mode during austral summer. Nevertheless, only the PSA2 mode was found to be a precursor for the following ENSO. It leads the SPQ-like SSTA by around one month, while the SAM and the PSA1 mode do not show any obvious linkage with either ENSO or the SPQ. This suggests that the PSA2 mode may provide a bridge between the preceding circulation anomalies over the extratropical SH and the following ENSO through the SPQ-like SSTA. | Jianhuang QIN Ruiqiang DING Zhiwei WU Jianping LI Sen ZHAO | 2017 | Advances in Atmospheric Sciences2017，34，3： | 1 |
| 11 | Choosing parameters of kernel subspace LDA for recognition of face images under pose and illumination variations显示文摘 | Huang Jian Chen Wensheng Lai Jianhuang | 2007 | IEEE Systems Man and Cybernetics Society2007，37，4： | 1 |
| 12 | A Novel Method for Conversion of Valuable BDS Intermediates HPBS/Cx-HPBS from DBT/Cx-DBT显示文摘A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfinate (HPBS) in the recombinant E. coli. Using GC/MS, HPBS accumulation was confirmed. The recombinant E. coli could also desulfurize Cx-DBT to corresponding Cx-HPBS. The result gave a new insight to BDS process and explored a new method to obtain valuable surfactants from cheap raw materials. | BoYU JianHUANG XiaoFengCAI XiaoYongLIU CuiQingMA FuLiLI PingXU | 2005 | Chinese Chemical Letters2005，16，7： | 1 |
| 13 | Effects of preparation conditions on performance of carbon-supported nanosize Pt-Co catalysts for methanol electro-oxidation under acidic conditions显示文摘 | Jianhuang Zeng Jim Yang Lee | 2004 | Journal of Power Sources2004，，2： | 1 |
| 14 | Hemodynamic characterization of transient blood flow in right coronary arteries with varying curvature and side-branch bifurcation angles显示文摘 | Guiying Liu Jianhuang Wu Dhanjoo N. Ghista Wenhua Huang Kelvin K.L. Wong | 2015 | Computers in Biology and Medicine2015，，： | 1 |
| 15 | Spatial-temporal consistent labeling of tracked pedestrians across non-overlapping camera views 显示文摘 | Lian Guoyun Lai Jianhuang Zheng Weishi | 2011 | Pattern Recognition2011，44，5： | 1 |
| 16 | Multiexemplar affinity propagation显示文摘 | Wang Changdong Lai Jianhuang Ching Y | 2013 | IEEE Trans on Pattern Analysis and Machine Intelligence2013，35，9： | 1 |
| 17 | Nonlinear nonnegative matrix factorization based on Mercer kernel construction显示文摘 | PAN Binbin LAI Jianhuang CHEN Wensheng | 2011 | Pattern Recognition2011，44，1011： | 1 |
| 18 | More active PtRu/C catalyst for methanol electrooxidation by reversal of mixing sequence in catalyst preparation 显示文摘 | Zeng Jianhuang Jim Yang Lee | 2007 | Materials Chemistry and Physics2007，104，： | 1 |
| 19 | Face recognition using holistic fourier invariant features显示文摘 | Lai Jianhuang YUEN P C Feng Guocan | 2001 | Pattern Recognition2001，34，1： | 1 |
| 20 | Perturbation LDA : Learning the difference between the class empirical mean and its expectation 显示文摘 | ZHENG Weishi LAI Jianhuang PUEN P C | 2009 | Pattern Recognition2009，42，5： | 1 |
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