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| 1 | Codon 249 mutation in exon 7 of p53 gene in plasma DNA:maybe a new early diagnostic marker of hepatocellular carcinoma in Qidong risk area,China显示文摘AIM: One of the characteristics of hepatocellular carcinoma (HCC) in Qidong area is the selective mutation resulting in a serine substitution at codon 249 of the p53 gene (1,20),and it has been identified as a 'hotspot' mutation in heptocellular carcinomas occurring in populations exposed to aflatoxin and with high prevalence of hepatitis B virus carriers (2, 3, 9, 10, 16, 24). We evaluated in this paper whether this 'hotspot' mutation could be detected in cellfree DNA circulating in plasma of patients with hepatocellular carcinoma and cirrhosis in Qidong, China, and tried to illustrate the significance of the detection of this molecular biomarker.METHODS: We collected blood samples from 25hepatocellular carcinoma patients, 20 cirrhotic patients and 30 healthy controls in Qidong area. DNA was extracted and purified from 200 μl of plasma from each sample. The 249ser p53 mutation was detected by restriction digestion analysis and direct sequencing of exon-7 PCR products.RESULTS: We found in exon 7 of p53 gene G→T transversion at the third base of codon 249 resulting 249Arg→249ser mutation in 10/25 (40%) hepatocellular carcinoma cases,4/20 (20%) cirrhotics, and 2/30 (7 %) healthy controls.The adjusted odds ratio for having the mutation was 22.1(95 % CI, 3.2~91.7) for HCC cases compared to controls.CONCLUSION: These data show that the 249ser p53mutation in plasma is strongly associated with hepatocellular carcinoma in Qidong patients. We found this mutation was also detected, although it was at a much lower frequency,in plasma DNA of Qidong cirrhotics and healthy controls;We consider that these findings, together with the usual method of HCC diagnosis, will give more information in early diagnosis of HCC, and 249ser p53 mutation should be developed to a new early diagnostic marker for HCC. | Xing-HuaHuang Lu-HongSun Dong-DongLu YanSun Li-JieMa Xi-RanZhang JianHuang LongYu | 2003 | World Journal of Gastroenterology2003，9，4： | 18 |
| 2 | Cloning and Overexpression of CYP6F1,a Cytochrome P450 Gene,from Deltamethrin-resistant Culex pipiens pallens显示文摘CYP6F1 (GenBank/EMBL accession No. AY662654), a novel gene with a complete encoding sequence in the cytochrome P450 family 6, was cloned and sequenced from deltamethrin-resistant 4th instar larvae of Culex pipiens pallens. The cDNA sequence of CYP6F1 has an open reading frame of 1527 bp, which encodes a putative protein of 508 amino acid residues. The deduced amino acid sequence of CYP6F1 indicated that the encoded P450 has conserved domains of a putative membrane-anchoring signal,putative reductase-binding sites, a typical heme-binding site, an ETLR motif and substrate recognition sites.Semi-quantitative RT-PCR analysis indicated that the CYP6F1 gene was expressed to a greater extent in the deltamethrin-resistant strain than in the susceptible strain of Cx. pipiens pallens. The expression levels of the CYP6F1 gene in the deltamethrin-resistant 1 st, 2nd, 3rd, 4th instar larvae and adult female mosquitoes differed, with highest expression levels in the 4th instar larvae. In addition, the CYP6F1 gene was stably expressed in mosquito C6/36 cells, and the expected 61.2 kDa band was identified by Western blotting. The cells transfected with CYP6F1 had an increased resistance to deltamethrin as compared with control cells.These results indicate that CYP6F1 is expressed at higher levels in the deltamethrin-resistant strain, and may confer some insecticide resistance in Cx. pipiens pallens. | Mao-QingGONG YanGU Xiao-BangHU YanSUN LeiMA Xiu-LanLI Li-XinSUN JingSUN | 2005 | Acta Biochimica et Biophysica Sinica2005，37，5： | 10 |
| 3 | Effects of Long-term Creep at Elevated Temperatures on Rupture Ductility in 9Cr-1Mo-V-Nb-N Steel显示文摘Besides the creep strength, creep ductility has also been recognized as an important material parameter, since low ductility is often associated with notch sensitivity and concomitant prema-ture failure, especially brittle fracture. Creep rupture data of 9Cr-1Mo-V-Nb-N show that the creep ductility decreases with decreasing stress at constant temperatures or with decreasing tem-perature at constant stresses. SEM and TEM were used to investigate the mechanism of creep brittleness. The results indicate that the decrease of rupture ductility after long-term creep is related to the decrease of mobile dislocation density, the coarsening and coalescence of carbides and the decrease of shear lip size. | Zhu, Lihui Zhao, Qinxin Gu, Haicheng Lu, Yansun
| 1998 | Journal of Materials Science & Technology1998，14，3： | 3 |
| 4 | Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma显示文摘 | CongfaHuang ZhijunSun YanSun XinmingChen XiaojieZhu ChengchaoFan BingLiu YifangZhao WenfengZhang | 2012 | Histopathology2012，，5： | 3 |
| 5 | Wavelet transform domain filters: a spatially selective noise filtration technique显示文摘 | Yansun Xu J 13 Weaver LI M Healy Jr el al | 1994 | IEEE Trans Image Processing1994，3，6： | 1 |
| 6 | Wavelet transform domain filters: a spatially selective noise filtration technique显示文摘 | Xu Yansun Weaver J B Healy D M | 1994 | IEEE Transaction on hnage Processing1994，3，6： | 1 |
| 7 | Simultaneous isolation of DNA, RNA, and protein from Medicago truncatula L.显示文摘 | JunboXiong QingchuanYang JunmeiKang YanSun TiejunZhang GruberMargaret WangDing | 2011 | ELECTROPHORESIS2011，，2： | 1 |
| 8 | Photopromoted Carbonylation of Alkyl Halides Under Ambient Conditions显示文摘Photopromoted carbonylation of alkyl halides with carbon monoxide can be carried out under ambient conditions with non-precious transition metal complexes (such as cobalt complexes) catalysts. Our preliminary work showed that alkyl halides can be transformed into alkene and alkane directly under irradiation, but the esters can not be transformed. It is assumed that the carbonylation of alkyl halides may be proceeded in two ways. | YanSUN JingMeiYIN DaBinGAO GuangYunZHOU YingPingJIA | 2003 | Chinese Chemical Letters2003，14，6： | 1 |
| 9 | The relationship between energy consumption structure,economic structure and energy intensity in China显示文摘 | FENG Taiwen LIN Yansun ZHANG Ying | | 0，，12： | 1 |
| 10 | Wavelet transforms domain filters: a spatial selective noise filtration technique显示文摘 | Xu Yansun Weaver J B Healy D M | 1994 | IEEE Transactions on Image Processing1994，3，6： | 1 |
| 11 | Wavelet transform domain filters: a spatially selective noise filtration technique 显示文摘 | XU YANSUN WEAVER J B HEALY D M Jr | 1994 | IEEE Transactions on Image Processing1994，3，6： | 1 |
| 12 | Electron-Phonon Coupling in the High-Pressure hcp Phase of Xenon: A First-Principles Study显示文摘 | YAO Yansun Tse J S | 2007 | PhysRevB2007，75，13： | 1 |
| 13 | Expression of nerve growth factor and tyrosine kinase receptor A and correlation with perineural invasion in pancreatic cancer显示文摘 | JunMa YumeiJiang YuhuaJiang YanSun XuemingZhao | 2008 | Journal of Gastroenterology and Hepatology2008，，12： | 1 |
| 14 | Superconductivity in Lithium under High Pressure Investigated with Density Functional and Eliashberg Theory 显示文摘 | YAO Yansun Tse J S Tanaka K | 2009 | Phys Rev B2009，79，05： | 1 |
| 15 | BH3 under Pressure: Leaving the Molecular Diborane Motit 显示文摘 | YAO Yansun Hotfmann R | 2011 | J Am Chem Soc2011，133，21： | 1 |
| 16 | Wavelet Transform Domain Filters: a Spatially Selective Noise Filtration Technique 显示文摘 | Yansun XU John B WEAVER Dennis M HEALY JR | 1994 | IEEE Transactions on Image Processing1994，3，6： | 1 |
| 17 | Wavelet Transform Domain Filters:A Spatially Selective Noise Filtration Technique显示文摘 | Yansun Xu John B | 1994 | IEEE TRANSACTIONS ON IMAGE PROCESSING1994，3，6： | 1 |
| 18 | Wavelet Transform Domain Filters:A Spatially Selective Noise Filtration Technique显示文摘 | Xu Yansun Weaver J B Healyd M | 1994 | IEEE Transactions on Image Processing1994，3，2： | 1 |
| 19 | Wavelet Transform Domain Filters:A Spatially Selective Noise Filtration Technique显示文摘 | XU Yansun WEAVER J B HEALY D M Jr | | 0，，06： | 1 |
| 20 | Filtering Noise from Images with Wavelet Transforms显示文摘 | Eli Weaver J YanSun x | 1991 | Magnetic Resonance in Medieine1991，，24： | 1 |
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