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| 1 | Nerve growth factor pretreatment against glutamate-induced hippocampal neuronal injury Action mechanism of phosphatase and tensin homologue deleted on chromosome 10显示文摘BACKGROUND: Nerve growth factor (NGF) attenuates glutamate-induced injury to hippocampal neurons, and the human tumor suppressor gene phosphatase and tensin homologue deleted on chromosome 10 (PTEN) promotes neuronal apoptosis. However, effects of PTEN in NGF-mediated neuroprotection against glutamate excitotoxicity remain poorly understood. OBJECTIVE: To investigate the relationship between NGF inhibition of glutamate-induced injury and PTEN. DESIGN, TIME AND SETTING: The randomized, controlled, in vitro study was performed at the Department of Pathophysiology, Medical School of Nantong University, China from October 2007 to March 2008. MATERIALS: Glutamate, NGF, 4, 6-diamidino-2-phenyl-indolediacetate, 3-[4, 5-dimethylthiazol-2-yl]- 2, 5-diphenyl tetrazoliumbromide (MTT), and lactate dehydrogenase kit (Sigma, USA), fluorescence microscope and inverted phase contrast microscope (Olympus, Japan) were used in this study. METHODS: Hippocampal neurons were obtained from newborn (< 24 hours) Sprague Dawley rats and cultured for 7 days. The control group was not treated with any intervention factor, the glutamate group was treated with glutamate (0.2 mmol/L), and NGF groups were treated with NGF (10, 50, 100, and 200 μg/L, respectively) prior to glutamate treatment. MAIN OUTCOME MEASURES: The MTT and lactate dehydrogenase assays were applied to evaluate viability of hippocampal neurons. Morphological changes in hippocampal neurons were observed using an inverted phase-contrast microscope, and neuronal apoptosis was detected by 4, 6-diamidino-2-phenyl-indolediacetate staining. PTEN mRNA and protein expression were measured by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. RESULTS: Glutamate (0.2 mmol/L) induced significantly decreased neuronal viability and greater lactate dehydrogenase efflux compared with the control group (P < 0.01). However, compared with the glutamate group, cell viability significantly increased and lactate dehydrogenase efflux decreased in the NGF group with increasing NGF concentrations (P < 0.05 or P < 0.01). The apoptotic ratio and PTEN mRNA and protein expression decreased in the NGF group compared with the glutamate group (P < 0.01). CONCLUSION: Pretreatment with NGF exerted neuroprotective effects against glutamate-induced injury, partially through inhibition of PTEN expression and neuronal apoptosis. | Yae Hu Jiahui Mao Yan Zhu Ailing Zhou | 2010 | Neural Regeneration Research2010，5，1： | 12 |
| 2 | Oxymatrine reduces neuroinflammation in rat brain A signaling pathway显示文摘Cerebral neuroinflammation models were established by injecting 10 μg lipopolysaccharide into the hippocampus of male Sprague-Dawley rats. The rats were treated with an intraperitoneal injection of 120, 90, or 60 mg/kg oxymatrine daily for three days prior to the lipopolysaccharide injection. Twenty-four hours after model induction, the hippocampus was analyzed by real-time quantitative PCR, and the cerebral cortex was analyzed by enzyme-linked immunosorbent assay and western blot assay. The results of the enzyme-linked immunosorbent assay and the real-time quantitative PCR showed that the secretion and mRNA expression of the pro-inflammatory cytokines interleukin-1β and tumor necrosis factor-α were significantly decreased in the hippocampus and cerebral cortex of model rats treated with oxymatrine. Western blot assay and real-time quantitative PCR analysis indicated that toll-like receptor 4 mRNA and protein expression were significantly decreased in the groups receiving different doses of oxymatrine. Additionally, 120 and 90 mg/kg oxymatrine were shown to reduce protein levels of nuclear factor-κB p65 in the nucleus and of phosphorylated IκBα in the cytoplasm of brain cells, as detected by western blot assay. Experimental findings indicate that oxymatrine may inhibit neuroinflammation in rat brain via downregulating the expression of molecules in the toll-like receptor 4/nuclear factor-κB signaling pathway. | Jiahui Mao Yae Hu Ailing Zhou Bing Zheng Yi Liu Yueming Du Jia Li Jinyang Lu Pengcheng Zhou | 2012 | Neural Regeneration Research2012，7，30： | 7 |
| 3 | Effect of Nao Yikang on choline acetyltransferase and caspase-3 brain expression in a rat model of Alzheimer's disease显示文摘BACKGROUND: The main components of the traditional Chinese medicine compound Nao Yikanghave been shown to possibly alleviate neural damage.OBJECTIVE: To observe the effects of Nao Yikang on expression of choline acetyltransferase(ChAT) and caspase-3 in the rat brains of an experimental Alzheimer's disease (AD) model, and toinvestigate the mechanisms of potential neuroprotective effects.DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at theDepartment of Pathophysiology, Medical School of Nantong University between November 2006and December 2007.MATERIALS: The main active components of Nao Yikang were as follows: prepared polygonummultiflorum, Rhizoma anemarrhenae, and Rhizoma acori tatarinowii. Nao Yikang granules wereprepared by Nantong Hospital of Traditional Chinese Medicine. Ibotenic acid (IBO) was purchasedfrom Sigma-Aldrich, USA, ChAT goat anti-rat antibody from Chemicon, USA, and cleaved caspase-3rabbit anti-rat (Asp175) (5A1) antibody from Cell Signaling, USA.METHODS: A total of 60 male, Sprague Dawley rats (2 months old) were randomly assigned to 6groups: sham-surgery, model, Nao Yikang 1.73, 3.45, 6.90 g/kg per day, and piracetam, with 10 ratsin each group. Bilateral infusions of 5 μg IBO into the nucleus basalis of Meynert were performedwith Hamilton syringe and stereotaxic apparatus for AD model establishment. For the sham-surgerygroup, rats received 1 μL saline in the identical stereotaxic position. From the second day, NaoYikang groups were administrated 1.73, 3.45, and 6.90 g/kg per day Nao Yikang, respectively, whilethe piracetam group received 0.04 g/mL piracetam, the model group received 0.5% sodiumcarboxymethyl cellulose, and the sham-surgery group received normal saline. Rats wereintragastrically administered 1 mL/100 g daily for 28 consecutive days.MAIN OUTCOME MEASURES: Following treatment of the various solutions for 28 days, Westernblot was utilized to observe ChAT expression in the frontal cortex of AD rats, andimmunohistochemistry was applied to quantify caspase-3-positive cells in the frontal cortex.RESULTS: ChAT protein expression significantly decreased in the model group (P < 0.01), howevercaspase-3 expression was significantly elevated (P < 0.01) compared with the sham-surgery group.Compared with the model group, ChAT protein expression increased in the Nao Yikang 1.73 g/kgper day, 3.45 g/kg per day, 6.90 g/kg per day groups, and the piracetam group (P < 0.05 or P < 0.01)and the number of caspase-3-positive cells decreased in the Nao Yikang 3.45 g/kg per day and6.90 g/kg per day groups (P < 0.01). However, there was no change in the number ofcaspase-3-positive cells in the 3.45 g/kg per day group.CONCLUSION: The traditional Chinese medicine compound Nao Yikangincreased ChAT proteinexpression and suppressed caspase-3 expression in the frontal cortex in a dose-dependent manner. | Jinsong Geng Hengjian Ni Jiancheng Dong Kui Jiang Ailing Zhou Yae Hu | 2009 | Neural Regeneration Research2009，4，12： | 3 |
| 4 | Role of Toll-like receptor 4 in inflammatory reactions of hippocampal neurons显示文摘Lipopolysaccharide stimulates Toll-like receptor 4 on immune cells to produce immune mediators. Toll-like receptor 4 is also expressed by non-immune cells, which can be stimulated by lipopolysaccharide. However, whether Toll-like receptor 4 is expressed by primary cultured hippocampal neurons and its specific role in lipopolysaccharide-induced neuroinflammation is currently undefined. In this study, Toll-like receptor 4 antibody blocking was used to analyze the Toll-like receptor 4 signaling pathway and changes in inflammation of lipopolysaccharide stimulated hippocampal neurons. Immunofluorescence showed that Toll-like receptor 4 protein was mainly located in the membrane of hippocampal neurons. Quantitative reverse transcription-PCR and western blot assay showed that after stimulation of lipopolysaccharide, the mRNA and protein levels of Toll-like receptor 4 and the mRNA levels of interleukin-1β and tumor necrosis factor-α were significantly increased. In addition, there was increased phosphorylation and degradation of kappa B α inhibitor in the cytosol and increased nuclear factor-κB p65 expression in the nuclei. Pretreatment with Toll-like receptor 4 antibody could almost completely block this increase. These experimental findings indicate that lipopolysaccharide participates in neuroinflammation by stimulating Toll-like receptor 4/nuclear factor-κB pathway in hippocampal neurons, which may be both 'passive victims' and 'activators' of neuroinflammation. | Yae Hu Jiahui Mao Yu Zhang Ailing Zhou | 2013 | Neural Regeneration Research2013，8，16： | 3 |
| 5 | Wide Band Gap Non-Fullerene Small Molecular Acceptors Containing Spirobifluorene and Benzotriazole with Three Different End-Capped Groups for P3HT-Based Organic Solar Cells显示文摘 | Xiaoyu Wen Bo Xiao Ailing Tang Junyi Hu Chunhe Yang Erjun Zhou | 2018 | Chinese Journal of Chemistry2018，36，5： | 2 |
| 6 | Label-free electrochemical immunosensor based on multi-functional gold nanoparticles-polydopamine-thionine-graphene oxide nanocomposites film for determination of alpha-fetoprotein显示文摘 | Peng Huaping Hu Yan Liu AiL in | 2014 | Journal of Electroanalytical Chemistry2014，712，1： | 1 |
| 7 | The Synthesis of SAPO-34 with Mixed Template and Its Catalytic Performance for Methanol to Olefins Reaction显示文摘 | Wang Pengfei Lü Ailing Hu Jie | 2012 | Microporous Mesoporous Mater2012，152，1： | 1 |
| 8 | Targeted delivery of large fusion protein into hippocampal neurons by systemic administration显示文摘 | Lixin Xiang Rumei Zhou Ailing Fu Xingran Xu Yuqi Huang Changhua Hu | 2011 | Journal of Drug Targeting2011，，8： | 1 |
| 9 | Synergistic effect of 2-oleyl-l-oleylarnidoethyl irrfidazoline atrnr:nium methylsulfate and halide ions on the inhibition of mild steel in HC1 显示文摘 | Hu Songqin4g Guo Ailing Gen4g Yufeng | 2012 | Materials Chemistry and Physics2012，134，： | 1 |
| 10 | A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants显示文摘The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years.The frequent emergence of immune escape variants have spurred the search for prophylactic vaccines and therapeutic antibodies that confer broad protection against SARS-CoV-2 variants.Here we show that the bivalency of an affinity maturated fully human singledomain antibody(n3113.1-Fc)exhibits exquisite neutralizing potency against SARS-CoV-2 pseudovirus,and confers effective prophylactic and therapeutic protection against authentic SARS-CoV-2 in the host cell receptor angiotensin-converting enzyme 2(ACE2)humanized mice.The crystal structure of n3113 in complex with the receptor-binding domain(RBD)of SARS-CoV-2,combined with the cryo-EM structures of n3113 and spike ecto-domain,reveals that n3113 binds to the side surface of up-state RBD with no competition with ACE2.The binding of n3113 to this novel epitope stabilizes spike in up-state conformations but inhibits SARS-CoV-2 S mediated membrane fusion,expanding our recognition of neutralization by antibodies against SARS-CoV-2.Binding assay and pseudovirus neutralization assay show no evasion of recently prevalent SARS-CoV-2 lineages,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)for n3113.1-Fc with Y58L mutation,demonstrating the potential of n3113.1-Fc(Y58L)as a promising candidate for clinical development to treat COVID-19. | Zhenlin Yang Yulu Wang Yujia Jin Yuanfei Zhu Yanling Wu Cheng Li Yu Kong Wenping Song Xiaolong Tian Wuqiang Zhan Ailing Huang Shanshan Zhou Shuai Xia Xiaoxu Tian Chao Peng Cuicui Chen Yibing Shi Gaowei Hu Shujuan Du Yuyan Wang Youhua Xie Shibo Jiang Lu Lu Lei Sun Yuanlin Song Tianlei Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 1 |
| 11 | Computer simulation of diffusion of corrosive particlein corrosion inhibitor membrane显示文摘 | Hu Songqing Guo Ailing Yah Youguo | 2011 | Computational and Theoretical Chemistry2011，964，： | 1 |
| 12 | Synergistic effect of 2-oleyl-1-oleylamidoethyl imidazoline ammonium methylsulfate and halide ions on the inhibition of mild steel in HCl显示文摘 | Songqing Hu Ailing Guo Yufeng Geng Xiaolin Jia Shuangqing Sun Jun Zhang | 2012 | Materials Chemistry and Physics2012，，1： | 1 |
| 13 | Willingness to communicate about organ donation among migrant workers in China's Mainland: a cross- sectional questionnaire survey显示文摘 | Wu Zhen Gao Lingling Hu Ailing Zhao Juanjuan Zhou Xiangfu | 2017 | 国际医药卫生导报2017，23，22： | 0 |
| 14 | Drug-peptide supramolecular hydrogel boosting transcorneal permeability and pharmacological activity via ligand-receptor interaction显示文摘Boosting transcorneal permeability and pharmacological activity of drug poses a great challenge in the field of ocular drug delivery.In the present study,we propose a drug-peptide supramolecular hydrogel based on anti-inflammatory drug,dexamethasone(Dex),and Arg-Gly-Asp(RGD)motif for boosting transcorneal permeability and pharmacological activity via the ligand-receptor interaction.The drug-peptide(Dex-SA-RGD/RGE)supramolecular hydrogel comprised of uniform nanotube architecture formed spontaneously in phosphate buffered saline(PBS,pH=7.4)without external stimuli.Upon storage at 4℃,25℃,and 37℃ for 70 days,Dex-SA-RGD in hydrogel did not undergo significant hydrolysis,suggesting great long-term stability.In comparison to Dex-SA-RGE,Dex-SA-RGD exhibited a more potent in vitro anti-inflammatory efficacy in lipopolysaccharide(LPS)-activated RAW 264.7 macrophages via the inhibition of nuclear factorкB(NF-κB)signal pathway.More importantly,using drug-peptide supramolecular hydrogel labeled with 7-nitro-2,1,3-benzoxadiazole(NBD),the Dex-SA-K(NBD)RGD showed increased performance in terms of integrin targeting and cellular uptake compared to Dex-SA-K(NBD)RGE,as revealed by cellular uptake assay.On topical instillation in rabbit’s eye,the proposed Dex-SA-K(NBD)RGD could effectively enhance the transcorneal distribution and permeability with respect to the Dex-SA-K(NBD)RGE.Overall,our findings demonstrate the performance of the ligand-receptor interaction for boosting transcorneal permeability and pharmacological activity of drug. | Lin Chen Jie Deng Ailing Yu Yuhan Hu Bo Jin Pengyuan Du Jianhong Zhou Lei Lei Yuan Wang Serhii Vakal Xingyi Li | 2022 | Bioactive Materials2022，7，4： | 0 |
| 15 | Field effect transistor-based tactile sensors: From sensor configurations to advanced applications显示文摘The past several decades have witnessed great progress in high-performance field effect transistors(FET)as one of the most important electronic compo-nents.At the same time,due to their intrinsic advantages,such as multiparameter accessibility,excellent electric signal amplification function,and ease of large-scale manufacturing,FET as tactile sensors for flexible wear-able devices,artificial intelligence,Internet of Things,and other fields to per-ceive external stimuli has also attracted great attention and become a significant field of general concern.More importantly,FET has a unique three-terminal structure,which enables its different components to detect external mechanics through different sensing mechanisms.On one hand,it provides an important platform to shed deep insights into the underlying mechanisms of the tactile sensors.On the other hand,these properties could in turn endow excellent components for the construction of tactile matrix sensor arrays with high quality.With special emphasis on the configuration of FETs,this review classified and summarized structure-optimized FET tactile sensors with gate,dielectric layer,semiconductor layer,and source/drain electrodes as sensing active components,respectively.The working principles and the state-of-the-art protocols in terms of high-performance tactile sensors are detail discussed and highlighted,the innovative pixel distribution and integration analysis of the transistor sensor matrix array concerning flexible electronics are also intro-duced.We hope that the introduction of this review can provide some inspiration for future researchers to design and fabricate high-performance FET-based tactile sensor chips for flexible electronics and other fields. | Jian Wang Shuyan Xu Congcong Zhang Ailing Yin Mingyuan Sun Hongru Yang Chenguo Hu Hong Liu | 2023 | InfoMat2023，5，1： | 0 |
| 16 | Identifying a confused cell identity for esophageal squamous cell carcinoma显示文摘The cell identity of malignant cells and how they acquire it are fundamental for our understanding of cancer.Here,we report that esophageal squamous cell carcinoma(ESCC)cells display molecular features equally similar but distinct to all three types of normal esophageal epithelial cells,which we term as confused cell identity(CCI).CCI is an independent prognostic marker associated with poor prognosis in ESCC.Further,we identify tropomyosin 4(TPM4)as a critical CCI gene that promotes the aggressiveness of ESCC in vitro and in vivo.And TPM4 creates CCI through activating the Jak/STAT-SOX2 pathway.Thus,our study suggests an unrecognized feature of ESCC cells,which might be of value for clinic prognosis and potential interference. | Xiangyu Pan Jian Wang Linjie Guo Feifei Na Jiajia Du Xuelan Chen Ailing Zhong Lei Zhao Lu Zhang Mengsha Zhang Xudong Wan Manli Wang Hongyu Liu Siqi Dai Ping Tan Jingyao Chen Yu Liu Bing Hu Chong Chen | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 0 |
| 17 | High fidelity virtual try-on network via semantic adaptation and distributed componentization显示文摘Image-based virtual try-on systems have significant commercial value in online garment shopping.However,prior methods fail to appropriately handle details,so are defective in maintaining the original appearance of organizational items including arms,the neck,and in-shop garments.We propose a novel high fidelity virtual try-on network to generate realistic results.Specifically,a distributed pipeline is used for simultaneous generation of organizational items.First,the in-shop garment is warped using thin plate splines(TPS)to give a coarse shape reference,and then a corresponding target semantic map is generated,which can adaptively respond to the distribution of different items triggered by different garments.Second,organizational items are componentized separately using our novel semantic map-based image adjustment network(SMIAN)to avoid interference between body parts.Finally,all components are integrated to generatethe overall result by SMIAN.A priori dual-modalinformation is incorporated in the tail layers of SMIAN to improve the convergence rate of the network.Experiments demonstrate that the proposed method can retain better details of condition information than current methods.Our method achieves convincing quantitative and qualitative results on existing benchmark datasets. | Chenghu Du Feng Yu Minghua Jiang Ailing Hua Yaxin Zhao Xiong Wei Tao Peng Xinrong Hu | 2022 | Computational Visual Media2022，8，4： | 0 |
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