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406篇 您的检索式:作者名="Helin"
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1Arabidopsis Transcription Factor Genes NF-YA 1, 5, 6, and 9 Play Redundant Roles in Male Gametogenesis, Embryogenesis, and Seed Development显示文摘原子因素 Y (NF-Y ) 是在所有真核细胞的有机体介绍的一个高度保存的抄写因素,并且是由三个子单元组成的 heterotrimer:NF -- 你, NF-YB,和 NF-YC。在 Arabidopsis,这三个子单元被 multigene 家庭编码。NF-Y 抄写因素的学习最好的成员是叶状的 COTYLEDON1 (LEC1 ) ,一个 NF-YB 家庭成员,哪个戏在 embryogenesis 的一个关键角色和种子成熟。然而,大多数 NF-Y 基因的功能留下逃犯。这里,我们在 NF 报导四基因的描述 -- 你家庭。我们发现 NF-YA1 的 gain-of-function 异种在男 gametogenesis 和 embryogenesis 显示出缺点。一致地, NF-YA1 的 overexpression, 5, 6,和 9 影响男 gametogenesis, embryogenesis,种子形态学,和种子萌芽,与更强壮的显型什么时候 overexpressing NF-YA1 和 NF-YA9。而且,这些 NF 的 overexpression -- 你基因也在不同的度在种子萌芽,延迟的幼苗生长,和迟了的 flowering 期间引起超敏性到 abscisic 酸(骆驼毛的织物) 。有趣地, NF-YA1 的 overexpression, 5, 6,和 9 是足够的从植物的纸巾导致体的胚胎的形成。然而,挑选或加倍这些 NF 的异种 -- 你基因没有可检测的显型。一起,这些结果提供证据那 NF-YA1, 5, 6,和 9 在男配偶体开发, embryogenesis,种子发展,和 germinative 以后生长起冗余的作用。Jinye Mu Helin Tan Sulei Hong Yan Liang Jianru Zuo 2013Molecular Plant2013,6,1:24
2Chinese urbanization 2050: SD modeling and process simulation显示文摘Is Chinese urbanization going to take a long time, or can its development goal be achieved by the government in a short time? What is the highest stable urbanization level that China can reach? When can China complete its urbanization? To answer these questions, this paper presents a system dynamic(SD) model of Chinese urbanization, and its validity and simulation are justified by a stock-flow test and a sensitivity analysis using real data from 1998 to 2013. Setting the initial conditions of the simulation by referring to the real data of 2013, the multi-scenario analysis from 2013 to 2050 reveals that Chinese urbanization will reach a level higher than 70% in 2035 and then proceed to a slow urbanization stage regardless of the population policy and GDP growth rate settings; in 2050, Chinese urbanization levels will reach approximately 75%, which is a stable and equilibrium level for China. Thus, it can be argued that Chinese urbanization is a long social development process that will require approximately20 years to complete and that the ultimate urbanization level will be 75–80%, which means that in the distant future, 20–25% of China's population will still settle in rural regions of China.GU ChaoLin GUAN WeiHua LIU HeLin 2017Science China Earth Sciences2017,60,6:16
3Anti-fouling ultrafiltration membrane prepared from polysulfone-graft-methyl acrylate copolymers by UV-induced grafting method显示文摘膜犯规是在膜面对的最重要的挑战之一极端过滤操作。压克力吃了的 polysulfone-graft-methyl 的共聚物被影响嫁接的度的同类的开始相片的接枝 copolymerization.The 变量综合,例如时间紫外(紫外可见) 照耀和压克力吃了的甲基和相片开始者的集中,被调查。接枝共聚物膜被阶段倒置方法准备。化学、词法的变化被红外线的光谱学(ATR/FT-TR ) ,扫描电子显微镜学,和水联系的稀释全部的 reflection-Fourier 变换描绘角度大小。结果表明甲基压克力吃了组在膜和压克力吃了的甲基的接枝度上是在场的膜的表演上的有的显著效果。有甲基压克力的增加的膜表面减少上的纯水接触角度吃了接枝度,它显示接枝共聚物膜的 hydrophilicity 被改进。纯水和牛的浆液白朊答案的浸透流动被测量评估对接枝共聚物膜,其结果显示出为接枝共聚物膜对性质犯规的反的改进的性质犯规的反。HUA Helin LI Na WU Linlin ZHONG Hui WU Guangxia YUAN Zonghuan LIN Xiangwei TANG Lianyi 2008Journal of Environmental Sciences2008,20,5:11
4Highly sensitive biosensors based on water-soluble conjugated polymers显示文摘Conjugated, conductive polymers are a kind of important organic macromolecules, which has found appli- cations in a variety of areas. The application of conjugated polymers in developing fluorescent biosensors represents the merge of polymer sciences and biological sciences. Conju- gated polymers are very good light harvesters as well as fluorescent polymers, and they are also “molecular wires”. Through elaborate designs, these important features, i.e. efficient light harvesting and electron/energy transfer, can be used as signal amplification in fluorescent biosensors. This might significantly improve the sensitivity of conjugated polymer-based biosensors. In this article, we reviewed the application of conjugated polymers, via either electron transfer or energy transfer, to detections of gene targets, an- tibodies or enzymes. We also reviewed recent efforts in con- jugated polymer-based solid-state sensor designs as well as chip-based multiple target detection. Possible directions in this conjugated polymer-based biosensor area are also dis- cussed.XUHui WUHaiping FANChunhai LIWenxin ZHANGZhizhou HELin 2004Chinese Science Bulletin2004,49,21:8
5Effects of Nitrogen Rate and Split Application Ratio on Nitrogen Use and Soil Nitrogen Balance in Cotton Fields显示文摘The Yellow River valley is one of the three largest cotton production areas in China.An experiment was performed in cotton fields of Anyang,China from 2013 to 2014 to investigate the effects of nitrogen(N) application rate and the ratio between basal and topdressing N fertilizer on N balance in a soil-plant system,N use efficiency,and cotton yield.Five N application rates as treatments were applied with the same split application ratio.Half of the N(50% basal fertilizer) was applied at pre-planting and the other half(50% topdressing fertilizer) at the initial flowering stage.These treatments were:zero N(N0,control),90 kg N ha^(-1)(N90(5/5)),180 kg N ha^(-1)(N180(5/5)),270 kg N ha^(-1)(N270(5/5),a reduced N rate),and 360 kg N ha^(-1)(N360(5/5),a conventional N rate).Additional 2 split application ratios as treatments were applied with the same N rate of 270 kg N ha^(-1).The split application ratios between basal N and topdressing N were 30%:70%(N270(3/7)) and 70%:30%(N270(7/3)).Results demonstrated that soil NH_4-N content in the 0–60 cm layer and NO3-N content in the 0–20 cm layer increased with increased N rate at the squaring and boll-opening stages and then decreased to lower levels at the initial flowering and harvest stages.Soil NO_3-N content in the 20–60 cm layer after the initial flowering stage increased with the increase of topdressing N rate.Soil apparent N surplus varied at different growth stages,while the soil apparent N surplus over the entire growth period exhibited a positive relationship at N rates over 180 kg ha^(-1).Seed cotton yield of N270(3/7) was the highest of all treatments.Plant N uptake,N agronomic efficiency,and apparent N recovery efficiency of N270(3/7) were significantly higher than those of N270(5/5) and N270(7/3) in both growing seasons.These suggest both economic and ecological benefits in cotton production in the Yellow River valley could be created,by appropriately reducing total N application rate and increasing the ratio of topdressing to basal N fertilizer at the initial flowering stage.LI Pengcheng DONG Helin LIU Aizhong LIU Jingran SUN Miao LI Yabing LIU Shaodong ZHAO Xinhua MAO Shuchun 2017Pedosphere2017,27,4:7
6Research and Application of Computer Management of the Test Chemical Products Quality Analysis显示文摘Hairui Zhang Guofu Zhang Helin Ye 2015Journal of Chemistry and Chemical Engineering2015,9,2:4
7Resistance Selection of Tetranychus cinnabarinus to Three Acaricides and Its Management Strategy显示文摘The effects of retarding resistance evolution of mixing and sequencing acaricides were studied based on the resistance selection of three acaricides to Tetranychus cinnabarinus in the laboratory.Tetranychus was continuously selected with fenpropathrin,abamectin and pyridaban for 28,30 and 31 generations,respectively,and resistance factor increased tp 65.6,7.2and 1.2 time,accordingly.Results of cross-resistance determination suggested that there were no cross-resistance existed among the three acaricides.Analysis of resisance selection and estimation of realized heritability(h^2)or resistance to three single and two mixed acaricides showed that a mixture of fenpropathrin+ abamectin would slow resistance development compared with a sequence of renpropathrin followed by abamectin while the mixture of pyridaben+abamectin and the sequence of pyridaben followed by abamectin would effectively retard resistance evolution.HELin ZHAOZhi-mo2003Agricultural Sciences in China2003,2,2:3
8Inhibition of GSK3B Bypass Drug Resistance of p53-Null Colon Carcinomas by Enabling Necroptosis in Response to Chemotherapy显示文摘Emanuela Grassilli Robert Narloch Elena Federzoni Leonarda Ianzano Fabio Pisano Roberto Giovannoni Gabriele Romano Laura Masiero Biagio Eugenio Leone Serena Bonin Marisa Donada Giorgio Stanta Kristian Helin Marialuisa Lavitrano 2013Clinical Cancer Research2013,,14:3
9Preparation and photoelectrochemical performance of TiO_2/Ag_2Se interface composite film显示文摘Coupling TiO2 with a narrow band gap semiconductor acting as the photosensitizer has attracted much attention in solar energy exploitation. In this work,the porous TiO2 film was first formed on the conducting glass plate (CGP) substrate by the decomposition of polyethylene glycol (PEG) mixing in titanium hydroxide sol at 450℃. Then,the TiO2/Ag2Se interface composite film was fabricated by interface reaction of AgNO3 with NaSeSO3 on the activated surface of porous TiO2 film. The results of SEM and XRD analyses indicated that the porous TiO2 layer was made up of the anatase crystal,and the Ag2Se layer was made up of congregative small particles that have low-temperature α-phase structure. Due to its efficient charge separation for the photo-induced electron-hole pairs,the TiO2/Ag2Se interface composite film as-prepared has good photovoltaic property and high photocurrent response for visible light,which have been confirmed by the photoelectrochemical measurements.ZHAO JuanJuan,JIANG BangTong,ZHANG ShengYi,NIU HeLin,JIN BaoKang & TIAN YuPeng Department of Chemistry,Anhui University,Hefei 230039,China 2009Science China Chemistry2009,52,12:3
10Optical coherence tomography angiography of the peripapillary region and macula in normal,primary open angle glaucoma,pseudoexfoliation glaucoma and ocular hypertension eyes显示文摘AIM:To evaluate and compare the peripapillary and retinal vasculature changes in primary open angle glaucoma(POAG),pseudoexfoliation glaucoma(PXG),ocular hypertension(OHT)and normal eyes using optical coherence tomography angiography(OCTA).METHODS:A total of 114 POAG,PXG and OHT eyes of 60 patients and 46 eyes of 23 healthy control participants with good quality OCTA images were included.The PXG,POAG,OHT,and control groups(aged 68.17±6.30 y,61.11±10.26 y,58.1±8.9 y,and 56.9±4.6 y,respectively)contained of 46,36,32,and 46 eyes,respectively.Measurements of vessel density(VD)in the peripapillary region and macula,average retinal inner thickness,and retinal nerve fiber layer thickness(RNFLT)were compared among groups.In order to test the accuracy of differentiation between eyes with and without glaucoma,the area was calculated under the receiver operating characteristic(ROC)curves.RESULTS:The VD in glaucomatous eyes was significantly lower than the control group in all peripapillary sectors(44.35%±6.78%vs 50.47%±1.83%,P<0.001),the superficial(44.08%±5.46%vs 51.28%±2.85%,P<0.001)and the deep(45.13%±8.55%vs 54.20%±5.44%,P<0.001)vascular plexus.There was a significant difference in peripapillary VD between glaucomatous and OHT eyes(44.35%±6.78%vs 49.86%±2.45%,P<0.001).The OHT group featured a lower superficial(48.06%±4.32%vs 51.28%±2.85%,P=0.027)and deep plexus(48.70%±5.99%vs 54.20%±5.44%,P=0.013)whole image vessel density(wiVD)than did the control group.The average macular superficial plexus wiVD was significantly lower in eyes with PXG than in eyes with POAG(42.22%±5.36%vs 46.54%±5.56%,P=0.046).CONCLUSION:OCTA can measure reduced peripapillary and macular VD in eyes with glaucoma and OHT,and these results are correlated to functional and structural glaucomatous alterations.Peripapillary and macular superficial plexus VD is lower in eyes with PXG than in eyes with POAG.Furthermore,the OHT eyes demonstrate impaired macular vasculature in both superficial and deep plexus.Helin Ceren Kose Oya Tekeli 2020International Journal of Ophthalmology(English edition)2020,13,5:3
11Linking microbial community composition to farming pattern in selenium-enriched region:Potential role of microorganisms on Se geochemistry显示文摘Selenium(Se)is an essential micronutrient for lives.Indigenous microbial communities play an important role on Se geochemistry in soils.In this study,the microbial community composition and functions of 53 soil samples were investigated using high-throughput sequencing.Samples were divided into 3 groups with different farming types based on the measured geochemical parameters and microbial functional structures.Results indicated that putative Se related bacteria Bacillus,Dyella,Paenibacillus,Burkholderia and Brevibacillus were dominant in dryland plantation soils which were characterized with higher available Se and low contents of H_(2)O,total organic carbon(TOC),NH_(4)^(+) and NO_(2)^(-).In contrast,the putative denitrifier Pseudomonas dominated in flooded paddy soils with higher TOC,NO_(3)^(-) and organic Se,whereas genera Rhizobium,Nitrosospira,and Geobacter preferred woodland soils with higher oxidation-reduction potential(ORP),pH,NH_(4)^(+) and Fe.Farming patterns resulted in distinct geochemical parameters including moisture,pH,ORP,TOC,and contents of soluble Fe,NO_(2)^(-) and NH_(4)^(+),shaping the microbial communities,which in turn affected Se forms in soils.This study provides a valuable insight into understanding of Se biogeochemistry in soils and prospective strategy for Se-rich agriculture production.Yanhong Wang Xinyan Shi Xianxin Huang Chunlei Huang Helin Wang Hanqin Yin Yixian Shao Ping Li 2022Journal of Environmental Sciences2022,34,2:3
12Linearly programmed DNA-based molecular computer operated on magnetic particle surface in test-tube显示文摘The postgenomic era has seen an emergence of new applications of DNA manipulation technologies, including DNA-based molecular computing. Surface DNA computing has already been reported in a number of studies that,however, all employ different mechanisms other than automaton functions. Here we describe a programmable DNA surface-computing device as a Turing machine-like finite automaton. The laboratory automaton is primarily composed of DNA (inputs, output-detectors, transition molecules as software), DNA manipulating enzymes and buffer system that solve artificial computational problems autonomously. When fluoresceins were labeled in the 5' end of (-) strand of the input molecule, direct observation of all reaction intermediates along the time scale was made so that the dynamic process of DNA computing could be conveniently visualized. The features of this study are: (i) achievement of finite automaton functions by linearly programmed DNA computer operated on magnetic particle surface and (ii)direct detection of all DNA computing intermediates by capiilary electrophoresis. Since DNA computing has the massive parallelism and feasibility for automation, this achievement sets a basis for large-scale implications of DNA computing for functional genomics in the near future.ZHAOJian ZHANGZhizhou SHIYongyong LiXiuxia HELin 2004Chinese Science Bulletin2004,49,1:3
13Effects of drip irrigation modes on growth and physiological characteristics of Arabica coffee under different N levels显示文摘The objective of this study was to obtain the water-saving and efficient production mode of Arabica coffee. The effects of three drip irrigation modes,conventional drip irrigation( CDI),alternate drip irrigation( ADI) and fixed drip irrigation( FDI) on growth,photosynthetic characteristics,biomass accumulation and irrigation water use efficiency of Arabica coffee were investigated under three nitrogen levels,high nitrogen( NH),middle nitrogen( NM) and low nitrogen( NL). The results show that there was a significant Logistic curve between the plant height,the stem diameter of Arabica coffee and growth days. Compared with CDI,ADI had no significant effects on leaf net photosynthetic rate,stomatal conductance,instantaneous water use efficiency and biomass accumulation above ground of Arabica coffee,while FDI decreased significantly,ADI and FDI increased irrigation water use efficiency by 50. 59% and 32. 85%,respectively. Compared with NH,with the reduction of N application rate,net photosynthetic rate,stomatal conductance,biomass accumulation above ground and irrigation water use efficiency decreased by 6. 81%-12. 30%,13. 70%-22. 69%,9. 61%-16. 67% and 9. 78%-15. 64%,respectively. Compared with CDINH,ADINHdecreased net photosynthesis rate and the stomatal conductance not significantly,other treatments decreased by 9. 16%-19. 22%,14. 49%-32. 91%,and decreased biomass accumulation above ground by 8. 26%-27. 34% except ADINH,and increased irrigation water use efficiency by 16. 46%-60. 95% except CDINMand CDINL. Therefore,alternate drip irrigation under high N level( ADINH) is the best water and nitrogen coupling mode of young Arabica coffee tree for water efficiency.HAO Kun LIU Xiaogang HAN Zhihui WU Helin YU Ning LIU Yanwei YANG Qiliang 2017排灌机械工程学报2017,35,10:3
14Tumor suppressor ASXL1 is essential for the activation of INK4B expression in response to oncogene activity and anti-proliferative signals显示文摘ASXL1 变化经常在 hematological 肿瘤被发现,并且 Asxl1 的损失在老鼠支持 myeloid 转变。这里我们在 vivo 在在 Histone H2A (H2AK119ub1 ) 上的 mono-ubiquitylated 离氨酸 119 的 deubiquitylation 为 ASXL1-BAP1 建筑群支持一个角色的现在的数据。Polycomb 组蛋白质在正常开发期间控制 INK4B-ARF-INK4A 地点的表达式,部分地通过催化 H2AK119 的 mono-ubiquitylation。自从地点的激活, INK4B-ARF-INK4A 玩一设有自动保险装置机制免于 tumorigenesis,我们调查了 ASXL1 依赖的 H2A deubiquitylation 是否在它的激活起一个作用。有趣地,我们发现 ASXL1 明确地为 p15 响应 oncogenic 发信号和外来的 anti-proliferative 的 INK4B 发信号。因为我们发现 ASXL1 和 BAP1 两个都在 INK4B 地点被充实,我们的结果建议 INK4B 地点的激活要求 H2AK119ub1 的 ASXL1/BAP1-mediated deubiquitylation。一致地,我们 ASXL1 变化与被联系的结果表演降低 p15 INK4B 和在主要骨头髓房间的造血的祖先的一个 proliferative 优点,和在多重房间线的 ASXL1 的那弄空导致抵抗到生长禁止的信号。一起拿,这研究连接 INK4B 表示的调停 ASXL1 的 H2A deubiquitylation 和 transcriptional 激活到它的肿瘤 suppressor 函数。Xudong Wu Ida Holst Bekker-Jensen Jesper Christensen Kasper Dindler Rasmussen Simone Sidoli Yan Qi Yu Kong Xi Wang Yajuan Cui Zhijian Xiao Guogang Xu Kristine Williams Juri Rappsilber Casper Kaae Sonderby Ole Winther Ole N Jensens Kristian Helin 2015Cell Research2015,25,11:3
15The Synthesis of Pyridine 2,6-dicarboxylic Acid Using Microwave Irradiation显示文摘Guofu Zhang Qing Zhang Hairui Zhang Lin Bai Helin Ye Lingling Liu 2010Journal of Chemistry and Chemical Engineering2010,4,11:2
16Resistin induces insulin resistance by both AMPK-dependent and AMPK-independent mechanisms in HepG2 cells显示文摘Zhaofan Luo Ying Zhang Fangping Li Juan He Helin Ding Li Yan Hua Cheng 2009Endocrine2009,,1:2
17Delivery of pOXR1 through an injectable liposomal nanoparticle enhances spinal cord injury regeneration by alleviating oxidative stress显示文摘Oxidation resistance 1(OXR1)is regarded as a critical regulator of cellular homeostasis in response to oxidative stress.However,the role of OXR1 in the neuronal response to spinal cord injury(SCI)remains undefined.On the other hand,gene therapy for SCI has shown limited success to date due in part to the poor utility of conventional gene vectors.In this study,we evaluated the function of OXR1 in SCI and developed an available carrier for delivering the OXR1 plasmid(pOXR1).We found that OXR1 expression is remarkably increased after SCI and that this regulation is protective after SCI.Meanwhile,we assembled cationic nanoparticles with vitamin E succinate-graftedε-polylysine(VES-g-PLL)(Nps).The pOXR1 was precompressed with Nps and then encapsulated into cationic liposomes.The particle size of pOXR1 was compressed to 58 nm,which suggests that pOXR1 can be encapsulated inside liposomes with high encapsulation efficiency and stability to enhance the transfection efficiency.The agarose gel results indicated that Nps-pOXR1-Lip eliminated the degradation of DNA by DNase I and maintained its activity,and the cytotoxicity results indicated that pOXR1 was successfully transported into cells and exhibited lower cytotoxicity.Finally,Nps-pOXR1-Lip promoted functional recovery by alleviating neuronal apoptosis,attenuating oxidative stress and inhibiting inflammation.Therefore,our study provides considerable evidence that OXR1 is a beneficial factor in resistance to SCI and that Nps-Lip-pOXR1 exerts therapeutic effects in acute traumatic SCI.Jing Zhang Yao Li Jun Xiong Helin Xu Guanghen Xiang Mingqiao Fan Kailiang Zhou Yutian Lin Xiangxiang Chen Lin Xie Hongyu Zhang Jian Wang Jian Xiao 2021Bioactive Materials2021,6,10:2
18Hydrothermal Synthesis and Capacitance Property of Cobalt Sulfide/Graphene Oxide Nanocomposite显示文摘The cobalt sulfide/graphene oxide(CoS/GO) nanocomposite was synthesized by a simple hydrothermal reaction.The products as-synthesized were characterized by XRD,SEM,TEM,BET-BJH and TG.The electrochemical property and impedance of the CoS/GO nanocomposite were studied by cyclic voltammetry and EIS analysis,respectively.The results show that the presence of the GO enhances the electrode conductivity,and then improves the capacitance property of the CoS/GO nanocomposite.The galvanostatic charge/discharge measurement results show that the CoS/GO nanocomposite has a high specific capacitance(550Fg^(-1)) and long cycle life(over 1 000 cycles).韦莹 张胜义 NIU Helin MAO Changjie SONG Jiming JIN Baokang TIAN Yupeng 2017Journal of Wuhan University of Technology(Materials Science)2017,32,1:2
19Dendrimer-decorated nanogels:Efficient nanocarriers for biodistribution in vivo and chemotherapy of ovarian carcinoma显示文摘Nanomedicine has revolutionized disease theranostics by the accurate diagnosis and efficient therapy.Here,the PAMAM dendrimer decorated PVCL-GMA nanogels(NGs)were developed for favorable biodistribution in vivo and enhanced antitumor efficacy of ovarian carcinoma.By an ingenious design,the NGs with a unique structure that GMA-rich domains were localized on the surface were synthesized via precipitation polymerization.After G2 dendrimer decoration,the overall charge is changed from neutral to positive,and the NGs-G2 display the whole charge nature of positively charged corona and neutral core.Importantly,the unique architecture and charge conversion of NGs-G2 have a profound impact on the biodistribution and drug delivery in vivo.As a consequence of this alteration,the NGs-G2 as nanocarriers emerge the highly sought biodistribution of reduced liver accumulation,enhanced tumor uptake,and promoted drug release,resulting in the significantly augmented antitumor efficacy with low side effects.Remarkably,this finding is contrary to some reported work that the nanocarriers with positive charge have preferential liver uptake.Moreover,the NGs-G2 also displayed thermal/pH dual-responsive behaviors,excellent biocompatibility,improved cellular uptake,and stimuli-responsive drug release.Encouragingly,this work demonstrates a novel insight into the strategy for optimizing design,improving biodistribution and enhancing theranostic efficacy of nanocarriers.Xin Li Zhijun Ouyang Helin Li Chaolei Hu Pabitra Saha Lingxi Xing Xiangyang Shi Andrij Pich 2021Bioactive Materials2021,6,10:2
20Serum Cystatin C as a determinant of glomerular filtration rate in children显示文摘Helin I Axenram M Grubb A 1998Clin Nephrol1998,49,4:1
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