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| 1 | Revolutionizing immunology with single-cell RNA sequencing显示文摘The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis.Identifying heterogeneity of immune cells is the key to understanding the immune system.Advanced single-cell RNA sequencing(scRNA-seq)technologies are revolutionizing our ability to study immunology.By measuring transcriptomes at the single-cell level,scRNA-seq enables identification of cellular heterogeneity in far greater detail than conventional methods.In this review,we introduce the existing scRNA-seq technologies and present their strengths and weaknesses.We also discuss potential applications and future innovations of scRNA-seq in immunology. | Haide Chen Fang Ye Guoji Guo | 2019 | Cellular & Molecular Immunology2019，16，3： | 10 |
| 2 | Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer显示文摘Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for efficiently inducing mutations in the genome.However,ZFN-mediated targeting in pigs has rarely been achieved.Here,we used ZFNs to knock out the porcineα-1,3-galactosyl-transferase(GGTA1)gene,which generates Gal epitopes that trigger hyperacute immune rejection in pig-to-human transplantation.Primary pig fibroblasts were transfected with ZFNs targeting the coding region of GGTA1.Eighteen mono-allelic and four biallelic knockout cell clones were obtained after drug selection with efficiencies of 23.4%and 5.2%,respectively.The biallelic cells were used to produce cloned pigs via somatic cell nuclear transfer(SCNT).Three GGTA1 null piglets were born,and one knockout primary fibroblast cell line was established from a cloned fetus.Gal epitopes on GGTA1 null pig cells were completely eliminated from the cell membrane.Functionally,GGTA1 knockout cells were protected from complement-mediated immune attacks when incubated with human serum.This study demonstrated that ZFN is an efficient tool in creating gene-modified pigs.GGTA1 null pigs and GGTA1 null fetal fibroblasts would benefit research and pig-to-human transplantation. | BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin | 2014 | Science China(Life Sciences)2014，57，2： | 9 |
| 3 | Structural optimization of uniaxial symmetry non-circular bolt clearance hole on turbine disk显示文摘This study proposes a parameterized model of a uniaxial symmetry non-circular hole, to improve conventional circular bolt clearance holes on turbine disks. The profile of the model consists of eight smoothly connected arcs, the radiuses of which are determined by 5 design variables.By changing the design variables, the profile of the non-circular hole can be transformed to accommodate different load ratios, thereby improving the stress concentration of the area near the hole and that of the turbine disk. The uniaxial symmetry non-circular hole is optimized based on finite element method(FEM), in which the maximum first principal stress is taken as the objective function. After optimization, the stress concentration is evidently relieved; the maximum first principal stress and the maximum von Mises stress on the critical area are reduced by 30.39% and 25.34%respectively, showing that the uniaxial symmetry non-circular hole is capable of reducing the stress level of bolt clearance holes on the turbine disk. | Chen Qiuren Guo Haiding Zhang Chao Liu Xiaogang | 2014 | Chinese Journal of Aeronautics2014，27，5： | 7 |
| 4 | Spatiotemporal pattern of urban forest leaf area index in response to rapid urbanization and urban greening显示文摘Rapid urbanization and urban greening have caused great changes to urban forests in China. Understanding spatiotemporal patterns of urban forest leaf area index(LAI) under rapid urbanization and urban greening is important for urban forest planning and management. We evaluated the potential for estimating urban forest LAI spatiotemporally by using Landsat TM imagery. We collected three scenes of Landsat TM(thematic mapper)images acquired in 1997, 2004 and 2010 and conducted a field survey to collect urban forest LAI. Finally, spatiotemporal maps of the urban forest LAI were created using a NDVI-based urban forest LAI predictive model.Our results show that normalized differential vegetation index(NDVI) could be used as a predictor for urban forest LAI similar to natural forests. Both rapid urbanization and urban greening contribute to the changing process of urban forest LAI. The urban forest has changed considerably from 1997 to 2010. Urban vegetated pixels decreased gradually from 1997 to 2010 due to intensive urbanization.Leaf area for the study area was 216.4, 145.2 and173.7 km^2 in the years 1997, 2004 and 2010, respectively.Urban forest LAI decreased sharply from 1997 to 2004 and increased slightly from 2004 to 2010 because of numerous greening policies. The urban forest LAI class distributions were skewed toward low values in 1997 and 2004. Moreover, the LAI presented a decreasing trend from suburban to downtown areas. We demonstrate the usefulness of TM remote-sensing in understanding spatiotemporal changing patterns of urban forest LAI under rapid urbanization and urban greening. | Zhibin Ren Yunxia Du Xingyuan He Ruiliang Pu Haifeng Zheng Haide Hu | 2018 | Journal of Forestry Research2018，29，3： | 4 |
| 5 | A 10×10 deep ultraviolet light-emitting micro-LED array显示文摘In this work,we design and fabricate a deep ultraviolet(DUV)light-emitting array consisting of 10×10 micro-LEDs(μ-LEDs)with each device having 20μm in diameter.Strikingly,the array demonstrates a significant enhancement of total light output power by nearly 52%at the injection current of 100 mA,in comparison to a conventional large LED chip whose emitting area is the same as the array.A much higher(~22%)peak external quantum efficiency,as well as a smaller efficiency droop forμ-LED array,was also achieved.The numerical calculation reveals that the performance boost can be attributed to the higher light extraction efficiency at the edge of eachμ-LED.Additionally,the far-field pattern measurement shows that theμ-LED array possesses a better forward directionality of emission.These findings shed light on the enhancement of the DUV LEDs performance and provide new insights in controlling the light behavior of theμ-LEDs. | Huabin Yu Muhammad Hunain Memon Hongfeng Jia Haochen Zhang Meng Tian Shi Fang Danhao Wang Yang Kang Shudan Xiao Shibing Long Haiding Sun | 2022 | Journal of Semiconductors2022，43，6： | 3 |
| 6 | A nomogram for individualized estimation of survival among adult patients with adrenocortical carcinoma after surgery:a retrospective analysis and multicenter validation study显示文摘Background:Clinical outcome of adrenocortical carcinoma(ACC)varies because of its heterogeneous nature and reliable prognostic prediction model for adult ACC patients is limited.The objective of this study was to develop and externally validate a nomogram for overall survival(OS)prediction in adult patients with ACC after surgery.Methods:Based on the data from the Surveillance Epidemiology,and End Results(SEER)database,adults patients diagnosed with ACC between January 1988 and December 2015 were identified and classified into a training set,comprised of 404 patients diagnosed between January 2007 and December 2015,and an internal validation set,com-prised of 318 patients diagnosed between January 1988 and December 2006.The endpoint of this study was OS.The nomogram was developed using a multivariate Cox proportional hazards regression algorithm in the training set and its performance was evaluated in terms of its discriminative ability,calibration,and clinical usefulness.The nomogram was then validated using the internal SEER validation,also externally validated using the Cancer Genome Atlas set(TCGA,82 patients diagnosed between 1998 and 2012)and a Chinese multicenter cohort dataset(82 patients diag-nosed between December 2002 and May 2018),respectively.Results:Age at diagnosis,T stage,N stage,and M stage were identified as independent predictors for OS.A nomo-gram incorporating these four predictors was constructed using the training set and demonstrated good calibration and discrimination(C-index 95%confidence interval[CI],0.715[0.679-0.751]),which was validated in the internal validation set(C-index[95%CI],0.672[0.637-0.707]),the TCGA set(C-index[95%CI],0.810[0.732-0.888])and the Chi-nese multicenter set(C-index[95%CI],0.726[0.633-0.819]),respectively.Encouragingly,the nomogram was able to successfully distinguished patients with a high-risk of mortality in all enrolled patients and in the subgroup analyses.Decision curve analysis indicated that the nomogram was clinically useful and applicable.Conclusions:The study presents a nomogram that incorporates clinicopathological predictors,which can accurately predict the OS of adult ACC patients after surgery.This model and the corresponding risk classification system have the potential to guide therapy decisions after surgery. | Jianqiu Kong Junjiong Zheng Jinhua Cai Shaoxu Wu Xiayao Diao Weibin Xie Xiong Chen Chenyi Liao Hao Yu Xinxiang Fan Chaowen Huang Zhuowei Liu Wei Chen Qiang Lv Haide Qin Jian Huang Tianxin Lin | 2019 | Cancer Communications2019，39，1： | 3 |
| 7 | Association of chromosome 7 aneuploidy measured by fluorescence in situ hybridization assay with muscular invasion in bladder cancer显示文摘Background:The preoperative prediction of muscular invasion status is important for adequately treating bladder cancer(BC)but nevertheless,there are some existing dilemmas in the current preoperative diagnostic accuracy of BC with muscular invasion.Here,we investigated the potential association between the fluorescence in situ hybridization(FISH)assay and muscular invasion among patients with BC.A cytogenetic-clinical nomogram for the individualized preoperative differentiation of muscle-invasive BC(MIBC)from non-muscle-invasive BC(NMIBC)is also proposed.Methods:All eligible BC patients were preoperatively tested using a FISH assay,which included 4 sites(chromosome-specific centromeric probe[CSP]3,7,and 17,and gene locus-specific probe[GLP]-p16 locus).The correlation between the FISH assay and BC muscular invasion was evaluated using the Chi-square tests.In the training set,univariate and multivariate logistic regression analyses were used to develop a cytogenetic-clinical nomogram for preoperative muscular invasion prediction.Then,we assessed the performance of the nomogram in the training set with respect to its discriminatory accuracy and calibration for predicting muscular invasion,and clinica usefulness,which were then validated in the validation set.Moreover,model comparison was set to evaluate the discrimination and clinical usefulness between the nomogram and the individual variables incorporated in the nomogram.Results:Muscular invasion was more prevalent in BC patients with positive CSP3,CSP7 and CSP17 status(OR[95%CI],2.724[1.555 to 4.774],P<0.001;3.406[1.912 to 6.068],P<0.001 and 2.483[1.436 to 4.292],P=0.001,respectively).Radiologydetermined tumor size,radiology-determined clinical tumor stage and CSP7 status were identified as independent risk factors of BC muscular invasion by the multivariate regression analysis in the training set.Then,a cytogenetic-clinical nomogram incorporating these three independent risk factors was constructed and was observed to have satisfactory discrimination in the training(AUC 0.784;95%CI:0.715 to 0.853)and validation(AUC 0.743;95%CI:0.635 to 0.850)set.The decision curve analysis(DCA)indicated the clinical usefulness of our nomogram.In models comparison,using the receiver operator characteristic(ROC)analyses,the nomogram showed higher discriminatory accuracy than any variables incorporated in the nomogram alone and the DCAs also identified the nomogram as possessing the highest net benefits at wide range of threshold probabilities.Conclusion:CSP7 status was identified as an independent factor for predicting muscular invasion in BC patients and was successfully incorporated in a clinical nomogram combining the results of the FISH assay with clinical risk factors. | Xiayao Diao Jinhua Cai Junjiong Zheng Jianqiu Kong Shaoxu Wu Hao Yu Hao Huang Weibin Xie Xiong Chen Chengran Huang Lifang Huang Haide Qin Jian Huang Tianxin Lin | 2020 | Cancer Communications2020，40，4： | 3 |
| 8 | Tapering-induced enhancement of light extraction efficiency of nanowire deep ultraviolet LED by theoretical simulations显示文摘A nanowire(NW) structure provides an alternative scheme for deep ultraviolet light emitting diodes(DUV-LEDs)that promises high material quality and better light extraction efficiency(LEE). In this report, we investigate the influence of the tapering angle of closely packed Al Ga N NWs, which is found to exist naturally in molecular beam epitaxy(MBE) grown NW structures, on the LEE of NW DUV-LEDs. It is observed that, by having a small tapering angle, the vertical extraction is greatly enhanced for both transverse magnetic(TM) and transverse electric(TE) polarizations. Most notably, the vertical extraction of TM emission increased from 4.8% to 24.3%,which makes the LEE reasonably large to achieve high-performance DUV-LEDs. This is because the breaking of symmetry in the vertical direction changes the propagation of the light significantly to allow more coupling into radiation modes. Finally, we introduce errors to the NW positions to show the advantages of the tapered NW structures can be projected to random closely packed NW arrays. The results obtained in this paper can provide guidelines for designing efficient NW DUV-LEDs. | RONGHUI LIN SERGIO VALDES GALAN HAIDING SUN YANGRUI HU MOHD SHARIZAL ALIAS BILAL JANJUA TIEN KHEE NG BOON S.OOI XIAOHANG LI | 2018 | Photonics Research2018，6，5： | 3 |
| 9 | Rolling texture development in a dual-phase Mg-Li alloy:The role of temperature显示文摘Dual-phase Mg-Li alloys sheets were rolled at four different temperatures ranging from liquid nitrogen to 300℃to explore effect of rolling temperature on texture and mechanical properties of the material.Crystal plasticity simulation was utilized to illustrate the influence of slip activity on rolling texture development.The results show that the rolling texture is largely depended on deformation temperature.Unlike commercial Mg alloys,the critical resolved shear stress of basal slip inα-Mg phase of Mg-Li alloy decreased more significantly by increasing temperature compared to that of pyramidalslip.Enhancement of basal slip by increasing temperature triggered a decrease of split angle of basal poles for the double-peak texture.Prismaticslip largely enhanced by increasing temperature upon 200℃,which induced a wider orientation spread along the transverse direction.For theβ-Li phase,the promotion of{110}<111>slip system at elevated temperature triggered the enhancement of{211}<110>and{111}<211>texture components.The cryo-rolled sample exhibited the highest strength compared to the others due to a strong hardening behavior at this temperature.A two-stage hardening behavior was observed in these as-rolled dual-phase alloys.Strain transition at phase boundaries could be the reason for appearance of this two-stage hardening. | Xiaoyan Li Fei Guo Yanlong Ma Luyao Jiang Hongliang Lai Haiding Liu Dingfei Zhang Risheng Pei | 2023 | Journal of Magnesium and Alloys2023，11，8： | 2 |
| 10 | 饲料行业用工特点——兼论集团用工现状、困难与对策显示文摘改革开放以来,中国饲料业年平均发展速度保持在20%以上,已完成了从手工作坊式的生产到世界第二大饲料生产国的飞跃,成为我国重要的支柱产业之一。目前全国有饲料企业逾万家,从业人员50多万人,总产量突破1亿吨。在肯定成绩的同时,我们也要清醒地意识到,饲料行业的产品、 | HAID | 2010 | 广东饲料2010，19，8： | 2 |
| 11 | A Plant-Derived Flavonoid Inhibits Entry of All HCV Genotypes Into Human Hepatocytes显示文摘 | Sibylle Haid Alexandra Novodomská Juliane Gentzsch Christina Grethe Silvia Geuenich Dorothea Bankwitz Patrick Chhatwal Beate Jannack Thierry Hennebelle Francois Bailleul Oliver T. Keppler Marion Poenisch Ralf Bartenschlager Céline Hernandez Matthieu Lemas | 2012 | Gastroenterology2012，，1： | 2 |
| 12 | Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq显示文摘Recently,single-cell RNA-seq technologies have been rapidly updated,leading to a revolution in biology.We previously developed Microwell-seq,a cost-effective and high-throughput single cell RNA sequencing(scRNA-seq)method with a very simple device.Most cDNA libraries are sequenced using an expensive Illumina platform.Here,we present the first report showing combined Microwell-seq and BGI MGISEQ2000,a less expensive sequencing platform,to profile the whole transcriptome of 11,883 individual mouse adult adrenal gland cells and identify 18 transcriptionally distinct clusters.Moreover,we performed a single-cell comparative analysis of human and mouse adult adrenal glands to reveal the conserved genetic networks in these mammalian systems.These results provide new insights into the sophisticated adrenal gland hierarchy and provide a benchmark,low-cost strategy for highthroughput single-cell RNA study. | Shujing Lai Lifeng Ma Weigao E Fang Ye Haide Chen Xiaoping Han Guoji Guo | 2020 | Cell Regeneration2020，9，1： | 2 |
| 13 | Atlantoaxial fixation: overview of all techniques显示文摘 | Mummaneni PV Haid RW | 2005 | Neurosurg2005，4，： | 1 |
| 14 | Allograft implants for posterior lumbar interbody fusion:results comparing cylindrical dowels and impacted wedges显示文摘 | Barnes B Rodts GE Jr Haid RW Jr | 2002 | Neurosurg2002，51，5： | 1 |
| 15 | Type Ⅰ fracture of the odontoid process: implication for atlano-occiptal instability显示文摘 | Scott EW Haid RW Jr Peacee D | 1990 | J Neurosurg1990，72，3： | 1 |
| 16 | Lumbar interbody fusion :state - of - the - art technical advances 显示文摘 | Mummaneni PV Haid RW Rodts GE | 2004 | J Neurosurg Spine2004，1，1： | 1 |
| 17 | Abrupt climatic change during the last interglacial-glacial cycle in the polar north Atlantic显示文摘 | Haid M Dokken T Mikalsen G | 2001 | Marine Geology2001，176，： | 1 |
| 18 | Anterior cervical plating enhances arthredesis after discectomy and fusion withcortical allograft显示文摘 | Kaiser MG Haid RW Js Subach BR | 2002 | Neurosurgery2002，50，2： | 1 |
| 19 | Deficiency of inducible nitric oxide synthase protects against MPTP toxicity in vivo 显示文摘 | Dehmer T Lindenau J Haid S | 2000 | J Neurochem2000，74，5： | 1 |
| 20 | Surgical Interbody ResearchGroup-radiographic assessment of interbody fusion devices : fusioncriteria for 'anterior lumbar interbody fusion显示文摘 | Burkus JK Foley K Haid R | 2001 | Neurosurg Focus2001，10，4： | 1 |
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