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| 1 | Ability of bone graft substitutes to support the osteoprogenitor cells: An in-vitro study显示文摘AIM: To compare seven commercially available bone graft substitutes(BGS) in terms of these properties and without using any additional biological growth factors.METHODS: Porcine osteoprogenitor cells were loaded on seven commercially available BGS and allowed to proliferate for one week followed by osteogenic induction. Staining for live/dead cells as well as scanning electron microscopy(SEM) was carried out to determine viability and cellular binding. Further outcome measures included alkaline phosphatase(ALP) assays with normalisation for DNA content to quantify osteogenic potential. Negative and positive control experiments were carried out in parallel to validate the results.RESULTS: Live/dead and SEM imaging showed higher viability and attachment with β-tricalcium phosphate(β-TCP) than with other BGS(P < 0.05). The average ALP activity in nmol/mL(normalised value for DNA content in nmol/μg DNA) per sample was 657.58(132.03) for β-TCP, 36.22(unable to normalise) for calcium sulphate, 19.93(11.39) for the Hydroxyapatite/Tricalcium Phosphate composite, 14.79(18.53) for polygraft, 13.98(8.15) for the highly porous β-Tricalcium Phosphate, 5.56(10.0) for polymers, and 3.82(3.8) for Hydroxyapatite.CONCLUSION: Under the above experimental conditions, β-TCP was able to maintain better the viability of osteoprogenitor cells and allow proliferation and differentiation(P < 0.05). | Ziad Dahabreh Michalis Panteli Ippokratis Pountos Mark Howard Peter Campbell Peter V Giannoudis | 2014 | World Journal of Stem Cells2014，6，4： | 3 |
| 2 | Executive Summary: Heart Disease and Stroke Statistics—2012 Update: A Report From the American Heart Association显示文摘 | Véronique L. Roger Alan S. Go Donald M. Lloyd-Jones Emelia J. Benjamin Jarett D. Berry William B. Borden Dawn M. Bravata Shifan Dai Earl S. Ford Caroline S. Fox Heather J. Fullerton Cathleen Gillespie Susan M. Hailpern John A. Heit Virginia J. Howard Bret | 2012 | Circulation2012，，1： | 2 |
| 3 | Accuracy of Orbscan pachymetry measurements and DHG ultrasound pachymetry in primary laser in situ keratomileusis and LASIK enhancement procedures显示文摘 | Nader G Iskander Ellen Anderson Penno N.Timothy Peters Howard V Gimbel Maria Ferensowicz | 2001 | Journal of Cataract & Refractive Surgery2001，，5： | 2 |
| 4 | Enhanced induction of RPE lineage markers in pluripotent neural stem cells engrafted into the adult rat subretinal space 显示文摘 | Enzmann V Howard R M Yamauchi Y | 2003 | Invest Ophthalmol Vis Sci2003，44，12： | 1 |
| 5 | Does social media im- prove communication?Evaluating the NIOSH science blog显示文摘 | V Sublet C Spring J Howard National Institute of Oc- cupational Safety and Health | 2011 | Am J Ind Med2011，54，5： | 1 |
| 6 | The polyester polyurethanase gene (pue A) from Pseudomonas chlororaphis encodes a lipase 显示文摘 | STERN R V HOWARD G T | 2000 | FEMS Microbiol Lett2000，185，： | 1 |
| 7 | Developing a Tourism Impact Attitude Scale 显示文摘 | Lanktord V & Howard D | 1994 | Annals of Tourism Research1994，，21： | 1 |
| 8 | Overproduction of recombinant proteins in plants显示文摘 | EGELKROUT E RAJAN V HOWARD J A | 2012 | Plant Sci2012，184，3： | 1 |
| 9 | Developing a Tourism Impact Attitude Scale显示文摘 | Lankford S V Howard D R | 1994 | Annals of Tourism Research1994，21，01： | 1 |
| 10 | Growth characteristics of the human nasal septum 显示文摘 | Van Loosen J Van Zanten G A Howard C V | 1996 | Rhinology1996，34，2： | 1 |
| 11 | Two monoclonal an tibodies(cc17,cc29)recognizing an antigen(Bo5)on bovine T lymphocytes,analogous to human CD5显示文摘 | Howard C J,Parsons K P,Jones B V | 1988 | Vet Immunol1988，13，： | 1 |
| 12 | Risk of cardiovascular disease by hysterectomy status,with and without oophorectomy:the women's health initiative observational Study显示文摘 | Howard B V Kuller L Langer R | 2005 | Circulation2005，111，12： | 1 |
| 13 | Developing a Tourism Impact Attitude Scale显示文摘 | Lankford S V D Howard | 1994 | Annals of Tourism Research1994，21，1： | 1 |
| 14 | Developing a Tourism Impact Attitude Scale显示文摘 | Lankford S V Howard D R | 1994 | Annals of Tourism Research1994，21，1： | 1 |
| 15 | Does social media improve communication? Evaluating the NIOSH sci- ence blog显示文摘 | V Sublet C Spring J Howard National Institute of Occupational Safety and Health | 2011 | AmJ lnd Med2011，54，5： | 1 |
| 16 | An improved model for the turbulent PBL显示文摘 | CHENG Y CANUTO V M HOWARD A M | 2002 | Journal of the Atmospheric Sciences2002，，： | 1 |
| 17 | Developing a tourism impact attitude scale显示文摘 | Lankford S V Howard D R | 1994 | Annals of Tourism Research1994，21，1： | 1 |
| 18 | Olfactory neuroblastoma : past, present,and future显示文摘 | Lund V J Howard D Wei W | 2003 | Laryngoscope2003，113，： | 1 |
| 19 | Ocean turbulence,part I: one-point closure model momentum and heat vertical diffusivities 显示文摘 | CANUTO V M HOWARD A CHENG Y | 2001 | Journal of Physical Oceanography2001，31，6： | 1 |
| 20 | The role of midfaeial degloving in modem rhinological practice 显示文摘 | Howard D J Lund V J | 1999 | J Laryngol Otol1999，113，10： | 1 |
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