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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Berberine ameliorates renal injury in streptozotocin-induced diabetic rats by suppression of both oxidative stress and aldose reductase显示文摘背景 Berberine 是 Coptidis 根茎(CR ) 和外皮 phellodendri 的主要成分之一,在这研究,我们与糖尿病的 nephropathy (DN ) 在老鼠在肾的功能和它的可能的机制上调查了 berberine 的有益的效果。方法男 Wistar 老鼠被划分成三个组:正常,糖尿病的模型,和 berberine 处理组。在糖尿病的模型和 berberine 治疗组的老鼠被 intraperitonal 注射与 streptozotocin (STZ ) 导致到糖尿病。Glomerular 区域, glomerular 体积, fasting 血葡萄糖(FBG ) ,血脲氮(甜面包) ,浆液 creatinine (Cr ) 和为 24 个小时(UP24h ) 的尿蛋白质用商业地可得到的工具包被测量。同时, superoxide dismutase (草皮) 的活动,在浆液的 malondialdehyde (MDA ) 的内容, aldose reductase (AR ) 的活动和在肾的 AR mRNA 和蛋白质的表示被不同方法检测。结果显示出的结果 berberine 的那个口头的政府(200mg 牲汥瑡摥 ? ぐ?? | LIU Wei-hua HEI Zi-qing NIE Hong TANG Fu-tian HUANG He-qing LI Xue-juan DENG Yan-hui CHEN Shao-rui GUO Fen-fen HUANG Wen-ge CHEN Feng-ying LIU Pei-qing | 2008 | Chinese Medical Journal2008，，8： | 41 |
| 3 | Development of "Purple Endosperm Rice" by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System显示文摘Anthocyanins 在主食庄稼的 anthocyanin 生合成的高抗氧化剂活动,和工程例如米饭(Oryza sativa L.) ,能为改进人的健康提供支持健康的食物。然而,为 biofortification 设计新陈代谢的小径仍然保持困难,并且因为它的 biosynthetic 小径的复杂基因规章的网络,在米饭内乳设计 anthocyanin 生产的以前的尝试失败了。在这研究,我们为叠的 transgene 开发了一个高效率的向量系统并且使用了它在米饭内乳设计 anthocyanin 生合成。我们做了包含内乳特定的倡导者驾驶的八 anthocyanin 相关的基因(从从薄荷科植物的玉米和六结构的基因 ?的二规章的基因) 的构造,加为标记切除的一个可选的标记和基因。有这构造的米饭的转变产生了新奇 biofortified germplasm 紫色内乳瑞斯(用汉语的叫的 Zijingmi ) ,它在内乳有高 anthocyanin 内容和抗氧化剂活动。这 anthocyanin 生产源于 transgenes 和产生激活的表示(或改进) 13 内长的 anthocyanin 生合成基因的表示,那是 silenced 或在野类型的米饭内乳在底层表示。这研究为叠的 transgene 提供一个有效、万用的工具箱并且为一条复杂生物小径的成功的工程表明它的使用,建议为在工厂的农学的特点的合成生物学和改进的这个工具箱的潜在的用途。 | Qinlong Zhu Suize Yu Dongchang Zeng Hongmei Liu Huicong Wang Zhongtang Yang Xianrong Xie Rongxin Shen Jiantao Tan Heying Li Xiucai Zhao Qunyu Zhang Yuanling Chen Jingxing Guo Letian Chen Yao-Guang Liu | 2017 | Molecular Plant2017，10，7： | 47 |
| 4 | From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm显示文摘Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as Supplements.Astaxanthin,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human health.However,astaxanthin is not produced in most higher plants.Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,respectively.Transgneic overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity.These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice endosperm.This work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements. | Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu | 2018 | Molecular Plant2018，11，12： | 35 |
| 5 | 抗稻瘟病水稻材料谷梅2号中主效抗稻瘟病基因的成簇分布显示文摘应用由籼稻组合中 15 6 /谷梅 2号衍生的 30 4个重组自交系 ,构建了由 177个标记组成、覆盖 12条水稻染色体的连锁图谱 ,定位控制水稻稻瘟病抗性的主效基因。前期定位的控制对中国稻瘟菌菌系 92 183(小种ZC1 5)穗瘟抗性的基因Pi2 5 (t)的位置得到进一步确认 ,位于第 6染色体标记A7和RG4 5 6之间 ,与A7和RG4 5 6的遗传距离分别为 1.7和 1 5cM ;发现群体对菲律宾稻瘟菌菌系Ca89(4谱系 )的叶瘟抗性由单基因控制 ,将该暂命名为Pi2 6 (t)的基因定位于第 6染色体标记B10和R6 74之间 ,与B10和R6 74的遗传距离分别为 5 .7和 2 5 .8cM。两个基因座位上的抗病等位基因均来源于谷梅 2号 ,表明谷梅 2号中存在控制水稻稻瘟病抗性的基因簇。 | 吴建利 柴荣耀 樊叶杨 李德葆 郑康乐 Hei LEUNG 庄杰云 | 2004 | 中国水稻科学2004，18，6： | 29 |
| 6 | A SELF-ADAPTIVE TRUST REGION ALGORITHM显示文摘In this paper we propose a self-adaptive trust region algorithm.The trust region radius is updated at a varable rate according to the ratio between the actual reduction and the predicted reduction of the objective function,rather than by simply enlarging or reducing the original trust region radius at a constant rate.We show that this new algorithm preserves the strong convergence property of traditional trust region methods.Numerical results are also presented. | Long Hei (Institute of Computational Mathematics and Scientific/Engineering Computing, Academy ofMathematics and Systems Sciences, Chinese Academy of Sciences, Beijing 100080, China)(Department of Industrial Engineering and Management Sciences Northwestern University C2SO,2145 Sheridan Road Evanston, Illinois 60208, USA) | 2003 | Journal of Computational Mathematics2003，21，2： | 29 |
| 7 | Prognostic values of serum cystatin C and β2 microglobulin, urinary β2 microglobulin and N-acetyl-β-D-glucosaminidase in early acute renal failure after liver transplantation显示文摘在肝移植以后的背景尖锐肾的失败(ARF ) 与高死亡和病态被联系。早治疗学或预防的干预被早有效的预示的因素的缺乏妨碍。最近的研究显示那浆液 cystatin 铺平 C 和 2-microglobulin (132 MG ) 象一样尿 2 MG 和 N 乙酰 --D- glucosaminidase (唠叨) 将与早、温和的肾的缺陷在病人增加。在这研究,这些因素在接受了 orthotopic 肝移植( OLT )的病人在不同阶段期间被检测,并且他们在 OLT 以后预言早 ARF 的可行性也是 analyzed.Methods 没有,接待了的有正常的血脲氮(甜面包)和浆液 creatinine ( SCr )的六十个病人修改了骑在背肩上的肝移植veno静脉绕过有希望地被学习。血样品为浆液 2 MG (n=60 ) 的决心从病人被拉, SCr (n=60 ) 和在跟随 5 间隔的浆液 Cystatin C (n=39 ) :在操作前(到) ,在 anhepatic 阶段(T1 ) 前的 20 分钟,在 anhepatic (T2 ) 的 25 分钟,在 repeffusion (T3 ) 以后并且在操作(T4 ) 的结束的 60 分钟。尿 2 MG (n=-60 ) 并且唠叨(n=60 ) 也在跟随 3 间隔被检验:在操作前(到) ,在灌注(T3 ) 以后并且在操作(T4 ) 的结束的 60 分钟。根据 Rimola 在在操作以后的 24 个小时的 ARF 的 A 标准,所有病人被划分成二个组:ARF 组和 non-ARF 组。数据统计上被分析评估在六十个盒子中的十个显示出的 operation.Results 前与正常 SCr 和甜面包在病人在肝移植以后为早 ARF 认为这些因素是预示的因素的 feasibiliy ARF (16.7%) 。逻辑回归分析证明浆液的层次并且尿在操作前的 2 MG 以及浆液 cystatin C 在肝移植以后与早 ARF 被相关( P < 0.05 ),当仅仅浆液 cystatin 铺平时,在操作的结束的 C 和 Cr 与早 ARF 相关( P < 0.05 , P < 0.01 )在肝移植以后。浆液 2 MG, Cystatin C, SCr 并且尿在 ARF 组的 2 个 MG 层次在 non-ARF 组比那更多高(P < 0.05, P < 0.01 ) 。浆液 cystatin C 的正确、假的预兆的积极比率之间有重要差别,浆液并且尿在操作前的 2 个 MG 层次( P < 0.05 , P < 0.01 ),当仅仅 SCr 在手术的结束显示出这些组之间的重要差别时( P < 0.01 )结果揭示了的 .Conclusions 在在手术,和那肝移植前表明了正常 SCr 和甜面包的那些病人之中有潜在的肾的损坏,这能加重这损坏并且引起 ARF 。这里,我们提供了浆液 Cystatin C 的预示的价值, 2 MG,尿 2 MG 并且在肝移植以后与早尖锐的肾的失败在病人唠叨。 | HEI Zi-qing LI Xiao-yun SHEN Ning PANG Hong-yu ZHOU Shao-li GUAN Jian-qiang | 2008 | Chinese Medical Journal2008，，14： | 26 |
| 8 | Motion trajectory prediction based on a CNN-LSTM sequential model显示文摘Accurate monitoring the surrounding environment is an important research direction in the field of unmanned systems such as bio-robotics,and has attracted much research attention in recent years.The trajectories of surrounding vehicles should be predicted accurately in space and time to realize active defense and running safety of an unmanned system.However,there is uncertainty and uncontrollability in the process of trajectory prediction of surrounding obstacles.In this study,we propose a trajectory prediction method based on a sequential model,that fuses two neural networks of a convolutional neural network(CNN)and a long short-term memory network(LSTM).First,a box plot is used to detect and eliminate abnormal values of vehicle trajectories,and valid trajectory data are obtained.Second,the trajectories of surrounding vehicles are predicted by merging the characteristics of CNN space expansion and LSTM time expansion;the hyper-parameters of the model are optimized according to a grid search algorithm,which satisfies the double-precision prediction requirement in space and time.Finally,data from next generation simulation(NGSIM)and Creteil roundabout in France are taken as test cases;the correctness and rationality of the method are verified by prediction error indicators.Experimental results demonstrate that the proposed CNNLSTM method is more accurate and features a shorter time cost,which meets the prediction requirements and provides an effective method for the safe operation of unmanned systems. | Guo XIE Anqi SHANGGUAN Rong FEI Wenjiang JI Weigang MA Xinhong HEI | 2020 | Science China(Information Sciences)2020，63，11： | 24 |
| 9 | 水稻品种多样性遗传分析与稻瘟病控制(英文)显示文摘以 2个籼型杂交稻———汕优 6 3(A)和汕优 2 2 (B)、2个地方糯稻品种———黄壳糯 (C)和紫糯 (D)和 3个粳稻品种———合系 4 1(E)、楚粳 12 (F)和 812 6 (G)为材料进行抗病基因同源序列 (ResistanceGeneAnalogue ,RGA)遗传分析。结果表明 ,杂交稻品种间以及粳稻品种间的抗性遗传较为相似 ,其相似系数分别为 0 86和 0 84。糯稻品种间以及糯稻、杂交稻和粳稻间的抗性遗传差异较大 ,相似系数为 0 4 5。聚类分析表明 ,RGA结果与品种的系谱来源相吻合 ,与品种的田间抗性基本一致。根据品种的抗性遗传差异、农艺性状和经济性状的不同 ,在云南籼稻区的建水和石屏县以及温暖粳稻区的泸西县分别选用 5种 (A/C、A/D、B/C、B/D和A/B)和 2种 (E/C和E/F/G)不同的品种组合进行品种多样性混合间栽控制稻瘟病田间试验 ,结果表明 ,抗性遗传差异大 (相似性 :0 4 5~ 0 77)的 5个品种混合间栽组合对稻瘟病有极为显著的控制效果 ,尤其是在混合间栽中高度感病的优质地方稻品种稻瘟病的发病率、病情指数均有极显著的下降 ,防治效果达 5 4 4 7%~ 92 18% ;遗传差异较小 (相似性 :0 84~ 0 90 )的 2个混栽组合混栽对稻瘟病的控制效果不明显 ,稻瘟病的防治效果在 15 12 %~ 2 5 5 4 %。此外 。 | 朱有勇 孙雁 王云月 李炎 何月秋 何霞红 Christopher C.Mundt Tom W.Mew Hei Leung | 2004 | Acta Genetica Sinica2004，31，7： | 23 |
| 10 | 用于水稻突变体大量筛选的DNA微量快速提取法显示文摘介绍了一种高通量水稻DNA微量快速抽提法。该方法特别适用于精细作图群体和大型突变体库的筛选鉴定,不仅通量大、速度快,而且可以确保足够的DNA浓度和纯度。DNA质量可以确保一般的PCR扩增,包括SSR检测以及应用于TILLING(targetinginducedlocal lesionin genome)分析。 | 邱福林 王和和 陈洁 庄杰云 Hei LEUNG 程式华 吴建利 | 2006 | 中国水稻科学2006，20，3： | 20 |
| 11 | 一个新的水稻卷叶突变体的遗传分析与基因定位显示文摘水稻叶片发育异常的突变体是研究水稻叶片发育分子机理的重要材料.本研究在IR64的EMS突变体库中发现了一个新的卷叶突变体材料w32,在整个生育期过程叶片都表现高度卷曲.突变体w32与IR24、培矮64杂交构建F2群体进行遗传分析,结果表明,卷叶性状受一个隐性基因控制.选用w32/PA64F2群体中的1846个卷叶单株进行基因定位,在卷叶和正常叶的DNA池中筛选到两个多态性标记RM6697和RM20781,并确定该卷叶基因位于第7染色体短臂上,是一个尚未报道过的基因,暂命名为rl11(t).利用新发展的11个SSR标记和19个InDel标记,最终将rl11(t)基因定位在一个约52kb的区段上,为最终克隆该卷叶基因奠定了基础. | 施勇烽 陈洁 刘文强 黄奇娜 沈波 HEI Leung 吴建利 | 2009 | 中国科学(C辑)2009，39，4： | 17 |
| 12 | CO39近等基因系抗稻瘟病性分析显示文摘利用 14个稻瘟病菌菌株在温室分别接种和在自然病圃两个年度对 CO39近等基因系进行鉴定。结果表明 ,这套近等单基因系和近等多基因系具很好的鉴别能力。抗病基因 Pi- 3提供抗病性的时间较长 ,病斑数较少 。 | 何月秋 唐文华 Hei Leung RobertS.Zeigler | 2001 | 作物学报2001，27，6： | 16 |
| 13 | A Novel Rice bHLH Transcription Factor, DTD, Acts Coordinately with TDR in Controlling Tapetum Function and Pollen Development显示文摘Dear Editor, Male reproductive development is an essential biological process for flowering plants and crucial for crop seed production.Formation of the male reproductive organ,the anther,involves a number of developmental events,including stamen meristem specification,generation of sporogenous cells and their differentiation into microspore mother cells (MMCs),meiosis,microspore (pollen) maturation,and pollination (Ma,2005).The formation of microspores and their development into mature pollen grains require cooperative interactions between gametophytic (microspores) and sporophytic (anther wall) cells,with the innermost cell layer,the tapetum,playing the most crucial role (Ma,2005).Tapetal cells undergo degeneration by programmed cell death (PCD). | Chonghui Ji Heying Li Libin Chen Min Xie Fengping Wang Yuanling Chen Yao-Guang Liu | 2013 | Molecular Plant2013，6，5： | 14 |
| 14 | Reinfection rate and endoscopic changes after successful eradication of Helicobacter pylori显示文摘AIM:To determine the long-term outcomes regarding reinfection with Helicobacter pylori(H.pylori)and endoscopic changes after successful H.pylori eradication. METHODS:From June 1994 to January 2007,186 patients(M:F=98:88;mean age 50.0±11.4 years), in whom H.pylori had been successfully eradicated, were enrolled.The mean duration of follow up was 41.2±24.0 mo. RESULTS:H.pylori reinfection occurred in 58 patients (31.2%).The average annual reinfection rate was 9.1% per patient year.No recurrence of peptic ulcer was detected at the follow up endoscopy.There were no significant differences between the H.pylori eradication regimens for the reinfection rate and no significant differences in endoscopic findings between the H.pylorirecurred group and the H.pylori-cured group.CONCLUSION:The reinfection rate in Korea is 9.1% which represents a decreasing trend.There was no relationship between H.pylori infection status and changes in endoscopic findings.There was also no recurrence or aggravation of ulcers. | Kum Hei Ryu Sun Young Yi Youn Ju Na Su Jung Baik Su Jin Yoon Hae-Sun Jung Hyun Joo Song | 2010 | World Journal of Gastroenterology2010，16，2： | 14 |
| 15 | Effect of Astragalus membranaceus injection on the activity of the intestinal mucosal mast cells after hemorrhagic shock-reperfusion in rats显示文摘Background The mechanism of mucosal damage induced by ischemia-reperfusion (IR) after hemorrhagic shock is complex; mast cells (MC) degranulation is associated with the mucosal damage. Astragalus membranaceus can protect intestinal mucosa against intestinal oxidative damage after hemorrhagic shock, and some antioxidant agents could prevent MC against degranulation. This study aimed to observe the effects of astragalus membranaceus injection on the activity of intestinal mucosal mast cells (IMMC) after hemorrhage shock-reperfusion in rats Methods Thirty-two Wistar rats were randomly divided into the normal group, model group, low dosage group, (treated with Astragalus membranacaus injection, 10 g crude medication/kg) and high dosage group (treated with Astragalus membranacaus injection, 20 g crude medication/kg). The rat model of hemorrhagic shock-reperfusion was induced by hemorrhage for 60 minutes followed by 90 minutes of reperfusion. The animals were administrated with 3 ml of the test drug solution before reperfusion. At the end of study, intestinal pathology, ultrastructure of IMMC, and expression of tryptase were assayed. The levels of malondisldehyde (MDA), TNF-α, histamine, and superoxide dismutase (SOD) activity in intestine were detected, and the number of IMMC was counted. Results The Chiu’s score of the rats in the model group was higher than in other groups (P<0.01). The Chiu’s score in the high dosage group was higher than that in the low dosage group (P<0.05). Hemorrhage-reperfusion induced IMMC degranulation: Astragalus membranaceus injection attenuated this degranulation. Expression of tryptase and the number of IMMC in the model group increased compared with the other groups (P<0.01) and was significantly reduced by the treatments of Astragalus membranaceus injection at both doses. There was no significant difference between the two treatment groups (P>0.05). MDA content and concentration of TNF-α in the model group were higher than that in the other three groups (P<0.05), and the concentration of TNF-α in the low dosage group was higher than that in the high dosage group (P<0.05). SOD activity and the concentration of histamine in the model group were lower than the other three groups (P<0.05). There was a negative correlation betweenthe Chiu’s score and the concentration of histamine and a positive correlation between the Chiu’s score and the concentration of TNF-α and between the SOD activity and the concentration of histamine in the four groups (P<0.05). Conclusion Astragalus membranaceus injection may reduce the damage to small intestine mucosa by inhibiting the activated IMMC after hemorrhagic shock. | GAN Xiao-liang HEI Zi-qing Huang He-qing CHEN Li-xin LI Shang-rong CAI Jun | 2006 | Chinese Medical Journal2006，，22： | 13 |
| 16 | 水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位显示文摘通过EMS诱变籼稻品种IR64获得一个稳定遗传的淡褐色斑点叶突变体lbsl1(light brown spotted leaf 1)。在自然条件下,突变体播种后10~14d,叶片上出现淡褐色斑点,随后逐渐扩散至全叶,第1叶至剑叶上均有淡褐色斑,为全生育期性状。斑点性状的表达对株高、生育期、结实率和千粒重等农艺性状具有显著的影响。遗传分析结果表明,该淡褐色斑点叶性状受一个隐性核基因控制。将突变体lbsl1与正常叶色水稻Morobereken杂交构建F2定位群体,利用SSR标记,最终将该淡褐叶基因lbsl1(t)定位在第6染色体短臂上一个约130kb的区段上。定位的结果和发展的群体为该基因的进一步精细定位和克隆奠定了基础。 | 奉保华 杨杨 施勇烽 林璐 陈洁 黄奇娜 魏彦林 Hei LEUNG 吴建利 | 2012 | 中国水稻科学2012，26，3： | 12 |
| 17 | Kai Xin San ameliorates scopolamine-induced cognitive dysfunction显示文摘Kai Xin San(KXS, containing ginseng, hoelen, polygala, and acorus), a traditional Chinese herbal compound, has been found to regulate cognitive dysfunction; however, its mechanism of action is still unclear. In this study, 72 specific-pathogen-free male Kunming mice aged 8 weeks were randomly divided into a vehicle control group, scopolamine group, low-dose KXS group, moderate-dose KXS group, high-dose KXS group, and positive control group. Except for the vehicle control group and scopolamine groups(which received physiological saline), the doses of KXS(0.7, 1.4 and 2.8 g/kg per day) and donepezil(3 mg/kg per day) were gastrointestinally administered once daily for 2 weeks. On day 8 after intragastric treatment, the behavioral tests were carried out. Scopolamine group and intervention groups received scopolamine 3 mg/kg per day through intraperitoneal injection. The effects of KXS on spatial learning and memory, pathological changes of brain tissue, expression of apoptosis factors, oxidative stress injury factors, synapse-associated protein, and cholinergic neurotransmitter were measured. The results confirmed the following.(1) KXS shortened the escape latency and increased residence time in the target quadrant and the number of platform crossings in the Morris water maze.(2) KXS increased the percentage of alternations between the labyrinth arms in the mice of KXS groups in the Y-maze.(3) Nissl and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining revealed that KXS promoted the production of Nissl bodies and inhibited the formation of apoptotic bodies.(4) Western blot assay showed that KXS up-regulated the expression of anti-apoptotic protein Bcl-2 and inhibited the expression of pro-apoptotic protein Bax. KXS up-regulated the expression of postsynaptic density 95, synaptophysin, and brain-derived neurotrophic factor in the cerebral cortex and hippocampus.(5) KXS increased the level and activity of choline acetyltransferase, acetylcholine, superoxide dismutase, and glutathione peroxidase, and reduced the level and activity of acetyl cholinesterase, reactive oxygen species, and malondialdehyde through acting on the cholinergic system and reducing oxidative stress damage. These results indicate that KXS plays a neuroprotective role and improves cognitive function through reducing apoptosis and oxidative stress, and regulating synapse-associated protein and cholinergic neurotransmitters. | Yu-Min Xu Xin-Chen Wang Ting-Ting Xu Hong-Ying Li Shang-Yan Hei Na-Chuan Luo Hong Wang Wei Zhao Shu-Huan Fang Yun-Bo Chen Li Guan Yong-Qi Fang Shi-Jie Zhang Qi Wang Wei-Xiong Liang | 2019 | Neural Regeneration Research2019，14，5： | 11 |
| 18 | Epidemiological Analysis on Reported Hepatitis C Cases in China from 2012 to 2016显示文摘Viralhepatitisisanimportantchallengeto publichealthworldwide.AshepatitisBiswell controlledduetovaccination,thediseaseburden causedbythespreadofhepatitisChasbecome increasinglyprominent.HepatitisCisaninfectious diseasethatismainlyblood-borne.Therateof chronicity ranges from 55%to 85%after people are infected with the hepatitis C virus (HCV)[1-2]. Twenty yearsafterinfectioninthegeneralpopulation,the incidence of cirrhosis ranges from 5%to 15%[3]. | HEI Fa Xin YE Shao Dong DING Guo Wei PANG Lin WANG Xiao Chun LIU Zhong Fu | 2018 | Biomedical and Environmental Sciences2018，31，10： | 10 |
| 19 | Perioperative changes of ventricular function and three indicators of myocardial injury during orthotopic liver transplantation显示文摘 | HEI Zi-qing LIU De-zhao LUO Chen-fang LI Shang-rong MA Wu-hua LUO Gang-jian | 2006 | Chinese Medical Journal2006，，11： | 9 |
| 20 | Bifurcation and Chaos Analysis of Nonlinear Rotor System with Axial-grooved Gas-lubricated Journal Bearing Support显示文摘Axial-grooved gas-lubricated journal bearings have been widely applied to precision instrument due to their high accuracy, low friction, low noise and high stability. The rotor system with axial-grooved gas-lubricated journal bearing support is a typical nonlinear dynamic system. The nonlinear analysis measures have to be adopted to analyze the behaviors of the axial-grooved gas-lubricated journal bearing-rotor nonlinear system as the linear analysis measures fail. The bifurcation and chaos of nonlinear rotor system with three axial-grooved gas-lubricated journal bearing support are investigated by nonlinear dynamics theory. A time-dependent mathematical model is established to describe the pressure distribution in the axial-grooved compressible gas-lubricated journal bearing. The time-dependent compressible gas-lubricated Reynolds equation is solved by the differential transformation method. The gyroscopic effect of the rotor supported by gas-lubricated journal bearing with three axial grooves is taken into consideration in the model of the system, and the dynamic equation of motion is calculated by the modified Wilson-θ-based method. To analyze the unbalanced responses of the rotor system supported by finite length gas-lubricated journal bearings, such as bifurcation and chaos, the bifurcation diagram, the orbit diagram, the Poincaré map, the time series and the frequency spectrum are employed. The numerical results reveal that the nonlinear gas film forces have a significant influence on the stability of rotor system and there are the rich nonlinear phenomena, such as the periodic, period-doubling, quasi-periodic, period-4 and chaotic motion, and so on. The proposed models and numerical results can provide a theoretical direction to the design of axial-grooved gas-lubricated journal bearing-rotor system. | ZHANG Yongfang HEI Di Lü Yanjun WANG Quandai MüLLER Norbert | 2014 | Chinese Journal of Mechanical Engineering2014，27，2： | 9 |
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