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30篇 您的检索式:作者名="HE ShiPing"
    题名 作者 年代 出处 被引量
1Responses of soil respiration to simulated precipitation pulses in semiarid steppe under different grazing regimes显示文摘Aims Precipitation pulses and different land use practices(such as grazing)play important roles in regulating soil respiration and carbon balance of semiarid steppe ecosystems in Inner Mongolia.However,the interactive effects of grazing and rain event magnitude on soil respiration of steppe ecosystems are still unknown.We conducted a manipulative experiment with simulated precipitation pulses in Inner Mongolia steppe to study the possible responses of soil respiration to different precipitation pulse sizes and to examine how grazing may affect the responses of soil respiration to precipitation pulses.Methods Six water treatments with different precipitation pulse sizes(0,5,10,25,50 and 100 mm)were conducted in the ungrazed and grazed sites,respectively.Variation patterns of soil respiration of each treatment were determined continuously after the water addition treatments.Important Findings Rapid and substantial increases in soil respiration occurred 1 day after the water treatments in both sites,and the magnitude and duration of the increase in soil respiration depended on pulse size.Significantly positive relationships between the soil respiration and soil moisture in both sites suggested that soil moisture was the most important factor responsible for soil respiration rate during rain pulse events.The ungrazed site maintained significantly higher soil moisture for a longer time,which was the reason that the soil respiration in the ungrazed site was maintained relatively higher rate and longer period than that in the grazed site after a rain event.The significant exponential relationship between soil temperature and soil respiration was found only in the plots with the high water addition treatments(50 and 100 mm).Lower capacity of soil water holding and lower temperature sensitivity of soil respiration in the grazed site indicated that degraded steppe due to grazing might release less CO_(2) to the atmosphere through soil respiration under future precipitation and temperature scenarios.Shiping Chen Guanghui Lin Jianhui Huang Mao He 2008Journal of Plant Ecology2008,1,4:24
2The discovery of Palaeoproterozoic volcanic rocks in the Bulunkuoler Group from the Tianshuihai Massif in Xinjiang of Northwest China and its geological significance显示文摘In the Dabudaer region of the Tianshuihai Massif (Xinjiang, Northwest China), metavolcanic rocks within the mainly meta-sedimentary Bulunkuoler Group are basalt, basaltic andesite, and rhyolite. In situ zircon U-Pb LA-ICP-MS dating on rhyolite magmatic zircons yielded an age of 2481±14 Ma, interpreted as the eruption age of the Bulunkuoler Group volcanic rocks. The basalt and basaltic andesite are low-Al tholeiite series and the rhyolite is low-Al calc-alkaline series. All of rocks are charac-terized by enrichment of LREE and LILE (Rb, Th and Ba), depletion of P, Nb, Ta and Ti, and absence of Eu anomalies. The εNd(t) of basalts ranges from 3.14 to 4.88 indicating a depleted mantle source. The trace element signatures show that these magmas experienced intense crustal contamination during their ascent. Direct evidence for crustal contamination is the xenocrystic zircons in the ryholite, with ages back to ca. 3300 Ma. The primitive-mantle normalized and Zr/Y-Zr diagrams for the basic volcanic rocks show that they formed in an intra-continental tectonic setting. Combined with the previous studies and compared with North China Craton Neoarchean basic volcanic rocks, it is supposed that the Bulunkuoler Group volcanic rocks reflect the Palaeoproterozoic mantle magma underplating and interaction with felsic crust.JI WenHua LI RongShe CHEN ShouJian HE ShiPing ZHAO ZhenMing BIAN XiaoWei ZHU HaiPing CUI JiGang REN JuanGang 2011Science China Earth Sciences2011,54,1:22
3Infection of Schistosomiasis japanicum is likely to enhance proliferation and migration of human breast cancer cells:mechanism of action of differential expression of MMP2 and MMP9显示文摘Objective:To study whether the infection of Schistosomiasis japanicum(S.japanicum) is related to enhanced proliferation and migration of cancer cells,and the molecular mechanism pertains to cancer cell metastasis in human host.Methods:The gene of S.japanicum glutathione transferase(sjGST) cloned from 5.japanicum was expressed,purified and applied in a series of assays to explore the effect of sjGST on proliferation and migration of MDA-MB-435S,and the expression of MMP2 and MMP9.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S was also carried out.Results:Results showed that sjGST enhanced proliferation and migration in human breast cancer cell MDA-MB-435S signifycantly at 50-200 nM,but did not enhance them in human lung cancer cell A549.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S and A549 showed that GST was readily hound to the breast cancer cells,but showed almost no binding to human lung cancer cells.The assays for gelatinase activity showed that both MMP2 and MMP9 activities were increased significantly in the presence of sjGST(50-200 nM) in MDA-MB-435S, but they were not significant in A549.Conclusions:Our current results show strongly that S. japanicum GST binds to MDA-MB-435S probably via its i'eceptor,and enhances proliferation and migration of the cancer cells by up-regulatory expression of MMP2 and MMP9.Ya-Ling Lin Rakesh Ramanujum Shiping He 2011Asian Pacific Journal of Tropical Biomedicine2011,1,1:9
4Discovery of ~4.0 Ga detrital zircons in the Changdu Block,North Qiangtang,Tibetan Plateau显示文摘在 situ 锆石由 LA-ICP-MS 和 MC-ICP-MS 标明日期使用, 3981 的由岩屑形成的锆石 ??? 鮼铧?? ? 裦蒚铩 ????? 偃 ?? 鮼 ?? 郥趽 ????腘 吗??ShiPing He RongShe Li Chao Wang HongFei Zhang WenHua Ji PuSheng Yu PingYang Gu Chao Shi 2011Chinese Science Bulletin2011,56,7:9
5Effect of Silicon on Growth, Physiology, and Cadmium Translocation of Tobacco (Nicotiana tabacum L.) in Cadmium-Contaminated Soil显示文摘Silicon(Si) offers beneficial effect on plants under cadmium(Cd) stress such as promoting plant growth and increasing resistance to heavy metal toxicity. In this study, a pot experiment was performed to study the role of Si in alleviating Cd toxicity in tobacco(Nicotiana tabacum L.) plants on a yellow soil taken from Guiyang, China. Nine treatments consisting of three concentrations of Cd(0, 1, and 5 mg kg^(-1)) together with three Si levels(0, 1, and 4 g kg^(-1)) were established. Plant growth parameters, Cd concentration,and the malondialdehyde(MDA), chlorophyll, and carotenoid contents were determined 100 d after transplanting of tobacco seedlings.Application of exogenous Si enhanced the growth of tobacco plants under Cd stress. When 5 mg kg^(-1) Cd was added, Si addition at 1 and 4 g kg^(-1) increased root, stem, and leaf biomass by 26.1%–43.3%, 33.7%–43.8%, and 50.8%–69.9%, respectively, compared to Si addition at 0 g kg^(-1). With Si application, the transfer factor of Cd in tobacco from root to shoot under both 1 and 5 mg kg^(-1) Cd treatments decreased by 21%. The MDA contents in the Si-treated tobacco plants declined by 5.5%–17.1% compared to those in the non-Si-treated plants, indicating a higher Cd tolerance. Silicon application also increased the chlorophyll and carotenoid contents by 33.9%–41% and 25.8%–47.3% compared to the Cd only treatments. Therefore, it could be concluded that Si application can alleviate Cd toxicity to tobacco by decreasing Cd partitioning in the shoots and MDA levels and by increasing chlorophyll and carotenoid contents, thereby contributing to lowering the potential health risks of Cd contamination.LU Yingang MA Jun TENG Ying HE Juny Peter CHRISTIE ZHU Lingjia REN Wenjie ZHANG Manyun Shiping DENG 2018Pedosphere2018,28,4:7
6Vaccination against Schistosoma japonicum Infection by DNA Vaccine Encoding Sj22.7 Antigen显示文摘在鼠标的效果, recombinant 原生质标志 pcDNA3-Sj22.7 与调停 liposome 的方法习惯于 transfectHeLa 房间, Sj22.7 mRNA 和蛋白质的表达式用反向的抄写聚合酶链反应,钠 dodecylsulfate-polyacrylamide 凝胶电泳和蛋白质印迹被检验。然后, pcDNA3-Sj22.7 的能力在鼠标的种痘以后根据蠕虫减小率和鸡蛋减小率被分析免于 Schistosomajaponicum 挑战感染。特定的 IgG 抗体和 T 淋巴细胞增长反应的浆液层次也是坚定的。在挑战感染以后,驾驶 Sj22.7 的干扰素(IFN )-gamma andinterleukin (IL )-4 也被确定。结果证明 pcDNA3-Sj22.7 能表示 Sj22.7 mRNAand 在 vitro.Immunization 的蛋白质导致了29.70%的蠕虫减小率;鸡蛋减小率of47.25%(肝)并且51.73%(肠),并且25.90%的鸡蛋减小率(鸡蛋每女性),在 连接group.Enzyme 的免疫吸着剂试金和蛋白质印迹分析显示了的 pcDNA3-Sj22.7 建议重要反肥沃的正式就职那使对从与 pcDNA3-Sj22.7 使免疫的鼠标的 Sj22.7.Tlymphocytes 的产生特定的 IgG 显示出的鼠标免疫怒气房间的一种重要增长反应 torSj22.7.The 文化证明 IFN-gamma 的那分泌物增加了但是 IL-4decreased.The 结果显示由 pcDNA3-Sj22.7 的 DNA 种痘对足够对 S.japonicum 感染得到保护的免疫的重要层次。DNA 疫苗能导致重要细胞、体液的有免疫力的反应,并且显示占优势的 T 助手房间类型 1 类型免疫者回答,它在鼠标对挑战感染贡献保护的免疫。Gan DAI Shiping WANG Junlong YU Shaorui XU Xianchu PENG Zhuo HE Xueqin LIU Songhua ZHOU and Fen LIU Institute of Schistosomiasis Research.Xiangya School of Medicine,Central South University,Changsha 410078 China 2007Acta Biochimica et Biophysica Sinica2007,39,1:5
7Bimetallic nano-mushrooms with DNA-mediated interior nanogaps for high-efficiency SERS signal amplification显示文摘有为散布的提高表面的拉曼(重量的单位) 分析的 ultrasensitive 的明确的 nanogaps 抓住伟人诺言的一致包含银的金属 nanostructures。不过,有强壮、稳定的重量的单位的如此的 nanostructures 的直接合成表明极其质问的遗体。这里,我们与内部 nanogaps 为金银的 nano 蘑菇的直接合成报导一条调停 DNA 的途径。这些 nano 蘑菇的重量的单位紧张极其依赖于在金头和银帽子之间的 nanogap 的区域。我们发现 nanogaps 的形成由控制在金 nanoparticles 上标记搁浅单人赛的 DNA (ssDNA ) 的 6-carboxy-X-rhodamine (ROX ) 的表面密度是细微地悦耳的。我们与 1.0 的一个高重量的单位信号改进因素在高产量获得了 nano 蘑菇我们作为一项 retrofit 措施在 CHS 在用阶段变化材料(脉冲编码调制) 的热存储的采纳以后。结果显示那与融化 23 的温度使用脉冲编码调制 ? 訄吗??Jianlei Shen Jing Su Juan Yan Bin Zhao Dongfang Wang Siyi Wang Kun Li Mengmeng Liu Yao He Sanjay Mathur Chunhai Fan Shiping Song 2015Nano Research2015,8,3:4
8Fabrication of metal-organic framework-based nanofibrous separator via one-pot electrospinning strategy显示文摘Metal-organic framework(MOF)/polymer composites have attracted extensive attention in the recent years.However,it still remains challenging to efficiently and effectively fabricate these composite materials.In this study,we propose a facile one-pot electrospinning strategy for preparation of HKUST-1/polyacrylonitrile(PAN)nanofibrous membranes from a homogeneous stock solution containing HKUST-1 precursors and PAN.MOF crystallization and polymer solidification occur simultaneously during the electrospinning process,thus avoiding the issues of aggregation and troublesome multistep fabrication of the conventional approach.The obtained HKUST-1/PAN electrospun membranes show uniform MOF distribution throughout the nanofibers and yield good mechanical properties.The membranes are used as separators in Li-metal full batteries under harsh testing conditions,using an ultrathin Li-metal anode,a high mass loading cathode,and the HKUST-1/PAN nanofibrous separator.The results demonstrate significantly improved cycling performance(capacity retention of 83.1%after 200 cycles)under a low negative to positive capacity ratio(N/P ratio of 1.86).The improvement can be attributed to an enhanced wettability of the separator towards electrolyte stemmed from the nanofibrous structure,and a uniform lithium ion flux stabilized by the open metal sites of uniformly distributed HKUST-1 particles in the membrane during cycling.Congcong Chen Weidong Zhang He Zhu Bo-Geng Li Yingying Lu Shiping Zhu 2021Nano Research2021,14,5:4
9Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity.YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China 2007Science China(Life Sciences)2007,50,2:4
10A Nano-and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network显示文摘A novel nano-and micro-integrated protein chip(NMIPC)that can detect proteins with ultrahigh sensitivity has been fabricated.A microfl uidic network(μFN)was used to construct the protein chips,which allowed facile patterning of proteins and subsequent biomolecular recognition.Aqueous phase-synthesized,water-soluble fl uorescent CdTe/CdS core-shell quantum dots(aqQDs),having high quantum yield and high photostability,were used as the signaling probe.Importantly,it was found that aqQDs were compatible with microfluidic format assays,which afforded highly sensitive protein chips for cancer biomarker assays.Juan Yan Mei Hu Di Li Yao He Rui Zhao Xingyu Jiang Shiping Song Lianhui Wang Chunhai Fan 2008Nano Research2008,1,6:3
11A Preliminary Investigation on Phenotypic Characteristics of Phytophthora infestans from Guizhou Province显示文摘[Objective] This study aimed to investigate the phenotypic characteristics of Phytophthora infestans from Guizhou Province.[Method] The metalaxyl resistance,mating type and isozyme genotype of 292 P. infestans strains isolated from Guizhou Province were analyzed.[Result] Metalaxyl-resistant P. infestans strains were found in different areas of Guizhou Province. Resistant,moderately resistant and susceptible strains accounted for 35. 62%,33. 90% and 30. 48%,respectively. Both mating types A1 and A2 were found,which accounted for 65. 38% and 34. 62%,respectively. P. infestans strains isolated from Guizhou Province mainly belonged to Gpi genotypes 100 /100 and 100 /100 /111,accounting for 50. 68% and 43. 84%,respectively.[Conclusion]This study provides the theoretical basis for the prevention and control of potato late blight in Guizhou Province.Lu HUANG Xiaojun CHEN Haiyong HE Xuehui LIU Shiping WU 2016Agricultural Biotechnology2016,5,3:2
12Revealing the surface electronic structures of AIGaN deep-ultraviolet multiple quantum wells with lateral polarity domains显示文摘We report on the carrier dynamic and electronic structure investigations on AlGaN-based deep-ultraviolet multiple quantum wells (MQWs)with lateral polarity domains.The localized potential maximum is predicted near the domain boundaries by first-principle calculation,suggesting carrier localization and efficient radiative recombination.More importantly,lateral band diagrams of the MQWs are proposed based on electron affinities and valance band levels calculated from ultraviolet(UV)photoelectron spectroscopy.The proposed lateral band diagram is further demonstrated by surface potential distribution collected by Kelvin probe microscopy and the density-of-state calculation of energy bands.This work illustrates that lateral polarity structures are playing essential roles in the electronic properties of II nitride photonic devices and may provide novel perspective in the realization of high-efficiency UV emitters.WEI GUO LI CHEN HOUQIANG XU YINGDA QIAN MOHEB SHEIKHI JASON HOO SHIPING GUO LIANG XU JIANZHE LU FERAS ALQATARI XIAOHANG LI KAIYAN HE ZHE CHUAN FENG JICHUN YE 2020Photonics Research2020,8,6:2
13PlaD: A Transcriptomics Database for Plant Defense Responses to Pathogens, Providing New Insights into Plant Immune System显示文摘High-throughput transcriptomics technologies have been widely used to study plant transcriptional reprogramming during the process of plant defense responses, and a large quantity of gene expression data have been accumulated in public repositories. However, utilization of these data is often hampered by the lack of standard metadata annotation. In this study, we curated2444 public pathogenesis-related gene expression samples from the model plant Arabidopsis and three major crops(maize, rice, and wheat). We organized the data into a user-friendly database termed as PlaD. Currently, PlaD contains three key features. First, it provides large-scale curated data related to plant defense responses, including gene expression and gene functional annotation data.Second, it provides the visualization of condition-specific expression profiles. Third, it allows users to search co-regulated genes under the infections of various pathogens. Using PlaD, we conducted a large-scale transcriptome analysis to explore the global landscape of gene expression in the curated data. We found that only a small fraction of genes were differentially expressed under multipleconditions, which might be explained by their tendency of having more network connections and shorter network distances in gene networks. Collectively, we hope that PlaD can serve as an important and comprehensive knowledgebase to the community of plant sciences, providing insightful clues to better understand the molecular mechanisms underlying plant immune responses. PlaD is freely available at http://gffzzed113f2916314452h6nnowouncuvn60pp.ffgz.tsg.suse.edu.cn/plad/index.php or http://gffzz5f8709884ffc4e15h6nnowouncuvn60pp.ffgz.tsg.suse.edu.cn/plad/index.php.Huan Qi Zhenhong Jiang Kang Zhang Shiping Yang Fei He Ziding Zhang 2018Genomics, Proteomics & Bioinformatics2018,16,4:2
14Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles显示文摘OBJECTIVE: To investigate the metabolic pathogenesis in subjects with subjective tinnitus(ST)having kidney deficiency pattern(KDP)(ST/KDP) in terms of Traditional Chinese Medicine.METHODS: Three groups of subjects, including healthy individuals, subjects with ST/KDP, and subjects who were healthy initially and then developed ST/KDP one year later(healthy → ST/KDP),were recruited for this study. Serum metabolic profiles of all subjects were analyzed using ultra-performance liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The metabolic characteristics of the ST/KDP subjects were determined, and the corresponding biomarkers were predicted. The metabolomics data from the healthy → ST/KDP subjects were collected for further verification.RESULTS: Twelve metabolites in the ST/KDP subjects were different from those of the healthy control subjects. Of these metabolites, according to the prediction, except for octanoic acid, other metabolites might characterize ST/KDP. Ten metabolites at the outcome ST/KDP stage were different from those at the initial(control) stage. Through the comparison of these metabolites with the predicted metabolites, five common metabolites, including upregulated glutamate, serotonin, oroticacid and 8-oxoguanine, as well as downregulated taurine, were found. These common metabolites were significantly associated with canonical pathways including calcium signaling, γ-aminobutyric acid(GABA) receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.CONCLUSION: The metabolic pathogenesis in ST/KDP subjects was characterized by upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, additionally, perturbations of calcium signaling, GABA receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.Tan Yong Shen Shanshan Guan Fulan He Bing Lu Cheng Xiao Cheng Jiang Miao Zhao Ning Li Li Cheng Shiping Zu Xianpeng Zhang Weidong Liu Xinru Lü Aiping 2018Journal of Traditional Chinese Medicine2018,38,5:1
15Responses of the functional structure of soil microbial community to livestock grazing in the T ibetan alpine grassland显示文摘Yunfeng Yang Linwei Wu Qiaoyan Lin Mengting Yuan Depeng Xu Hao Yu Yigang Hu Jichuang Duan Xiangzhen Li Zhili He Kai Xue Joy van Nostrand Shiping Wang Jizhong Zhou 2012Glob Change Biol2012,,2:1
16Capability-based IoT access control using blockchain显示文摘Internet of Things(IoT)devices facilitate intelligent service delivery in a broad range of settings,such as smart offices,homes and cities.However,the existing IoT access control solutions are mainly based on conventional identity management schemes and use centralized architectures.There are knowm security and privacy limitations with such schemes and architectures,such as the single-point failure or surveillance(e.g.,device tracking).Hence,in this paper,we present an architecture for capability-based IoT access control utilizing the blockchain and decentralized identifiers to manage the identity and access control for IoT devices.Then,we propose a protocol to provide a systematic view of system interactions,to improve security.We also implement a proof-of-concept prototype of the proposed approach and evaluate the prototype using a real-world use case.Our evaluation results show that the proposed solution is feasible,secure,and scalable.Yue Liu Qinghua Lu Shiping Chen Qiang Qu Hugo’Connor Kim-Kwang Raymond Choo He Zhang 2021Digital Communications and Networks2021,7,4:1
17Functional nanoprobes for ultrasensitive detection of biomolecules 显示文摘Song Shiping Qin Yu He Yao 2010Chemical Society Re- views2010,39,11:1
18Seroprevalence and sources of Toxoplasma infection among indigenous and immigrant pregnant women in Taiwan显示文摘Ya-Ling Lin Yen-Shun Liao Long-Ren Liao Fei-Na Chen Hsiu-Maan Kuo Shiping He 2008Parasitology Research2008,,1:1
19Direct transformation of ZIF-8 into hollow porous carbons and hollow carbon composites显示文摘Hollow porous carbons(HPCs)are a class of porous materials with advantages of high surface to volume ratio,large interior cavities,low density,and short diffusion length,which are promising in various applications.Direct carbonization of carbon precursors is the simplest and the most cost-effective method to prepare porous carbons,however,it often leads to non-hollow structures.Herein,we demonstrate the preparation of HPCs through a direct carbonization method with a two-step heating process of zeolitic imidazolate framework-8(ZIF-8)and tetrafluoroterephthalonitrile(TFTPN).During the carbonization,ZIF-8 nanoparticles first react with TFTPN at low temperature to create polymer coatings on the surface,which are then converted into HPCs at elevated temperature.The obtained HPCs show hierarchically porous structure with high specific surface areas and pore volumes.Additionally,this method has been adopted to fabricate Au@HPCs yolk–shell composites,exhibiting good catalytic performance in nitrobenzene reduction.The developed synthesis strategy can enrich the toolbox for the preparation of novel HPCs and their composites.Lingqi Huang Zhiyong Luo Wenjie Han Qi Zhang He Zhu Shiping Zhu 2022Nano Research2022,15,7:1
20Synthesis and luminescence of Sr2CeO4 superfine particles by citrate-gel method显示文摘Xibin Yu Xianghong He Shiping Yang 2004Materials Letters2004,58,:1
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