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| 1 | Occurrence of human pathogenic bacteria carrying antibiotic resistance genes revealed by metagenomic approach: A case study from an aquatic environment显示文摘Antibiotic resistance genes(ARGs), human pathogenic bacteria(HPB), and HPB carrying ARGs are public issues that pose a high risk to aquatic environments and public health. Their diversity and abundance in water, intestine, and sediments of shrimp culture pond were investigated using metagenomic approach. A total of 19 classes of ARGs, 52 HPB species, and 7 species of HPB carrying ARGs were found. Additionally, 157, 104, and 86 subtypes of ARGs were detected in shrimp intestine, pond water, and sediment samples, respectively. In all the samples, multidrug resistance genes were the highest abundant class of ARGs. The dominant HPB was Enterococcus faecalis in shrimp intestine, Vibrio parahaemolyticus in sediments, and Mycobacterium yongonense in water, respectively. Moreover, E. faecalis(contig Intestine_364647)and Enterococcus faecium(contig Intestine_80272) carrying efr A, efr B and ANT(6)-Ia were found in shrimp intestine, Desulfosaricina cetonica(contig Sediment_825143) and Escherichia coli(contig Sediment_188430) carrying mex B and APH(3′)-IIa were found in sediments, and Laribacter hongkongensis(contig Water_478168 and Water_369477), Shigella sonnei(contig Water_880246),and Acinetobacter baumannii(contig Water_525520) carrying sul1, sul2, ere A, qac H, OXA-21, and mph D were found in pond water. Mobile genetic elements(MGEs) analysis indicated that horizontal gene transfer(HGT) of integrons, insertion sequences, and plasmids existed in shrimp intestine, sediment, and water samples, and the abundance of integrons was higher than that of other two MGEs. The results suggested that HPB carrying ARGs potentially existed in aquatic environments, and that these contributed to the environment and public health risk evaluation. | Renjun Zhou Shenzheng Zeng Dongwei Hou Jian Liu Shaoping Weng Jianguo He Zhijian Huang | 2019 | Journal of Environmental Sciences2019，31，6： | 5 |
| 2 | Integrated genomic analysis identifies deregulated JAK/STAT-MYC-biosynthesis axis in aggressive NK-cell leukemia显示文摘好攻击的 NK 房间白血病(ANKL ) 是在从亚洲的人之中是更流行的 NK 房间瘤的一种稀罕形式并且中央并且南美洲。病人们通常在天以内死到月,甚至在收到迅速的治疗学的管理以后。这里,我们由集成整个染色体执行了 ANKL 的第一全面研究, transcriptome 并且定序指向,象功能的试金一样的 cytokine 数组。在 JAK-STAT 小径的变化在 48% 被识别(14/29 ) ANKL 病人,当细胞外的 STAT3 激发器 IL10 被 56 褶层的一般水准提高时(P < 0.0001 ) 在检查的所有病人的血浆。另外的经常变异的基因包括的 TP53 (34%) , TET2 (28%) , CREBBP (21%) 和 MLL2 (21%) 。耐心的 NK 白血病房间在多重新陈代谢的小径显示出 STAT3 phosphorylation, MYC 表示和 transcriptional 活动的突出的激活。机能上地, STAT3 激活和 MYC 表示为 ANKL 房间的增长和幸存是批评的。发信号的 STAT 调整了发信号的 MYC 抄写节目,和两 STAT, MYC 抄写被要求维持核苷酸合成和 glycolysis 的激活。一起, JAK-STAT 小径在 ANKL 为 genomic 改变和 IL10 刺激代表一个主要目标。这最新发现的 JAK/STAT-MYC-biosynthesis 轴可以在对待白血病的这种子类型为新奇治疗学的策略的发展提供机会。 | Liang Huang Dan Liu Na Wang Shaoping Ling Yuting Tang Jun Wu Lingtong Hao Hui Luo Xuelian Hu Lingshuang Sheng Lijun Zhu Di Wang Yi Luo Zhen Shang Min Xiao Xia Mao Kuangguo Zhou Lihua Cao Lili Dong Xinchang Zheng Pinpin Sui Jianlin He Shanlan Mo Jin Yan Qilin Ao Lugui Qiu Hongsheng Zhou Qifa Liu Hongyu Zhang Jianyong Li Jie Jin Li Fu Weili Zhao Jieping Chen Xin Du Guoliang Qing Hudan Liu Xin Liu Gang Huang Ding Ma Jianfeng Zhou Qian-fei Wang | 2018 | Cell Research2018，28，2： | 5 |
| 3 | Application of a Support Vector Machine Algorithm to the Safety Precaution Technique of Medium-low Pressure Gas Regulators显示文摘In the gas pipeline system, safe operation of a gas regulator determines the stability of the fuel gas supply, and the medium-low pressure gas regulator of the safety precaution system is not perfect at the present stage in the Beijing Gas Group; therefore, safety precaution technique optimization has important social and economic significance. In this paper, according to the running status of the medium-low pressure gas regulator in the SCADA system, a new method for gas regulator safety precaution based on the support vector machine(SVM) is presented. This method takes the gas regulator outlet pressure data as input variables of the SVM model, the fault categories and degree as output variables, which will effectively enhance the precaution accuracy as well as save significant manpower and material resources. | HAO Xuejun AN Xaioran WU BO HE Shaoping | 2018 | Journal of Thermal Science2018，27，1： | 5 |
| 4 | Protective immunity of orange-spotted grouper(Epinephelus coioids) against a nervous necrosis virus isolated from China,and determination of the complete sequences of the virus显示文摘On the basis of the sequence and analysis of genome from the orange-spotted nervous necrosis virus(OGNNV), China strain, a pair of special primers were designed according to the nucleotide sequences of RNA2 from OGNNV. The major capsid protein(MCP)gene of OGNNV was cloned by means of reverse-transcriptase polymerase chain reaction (RT-PCR)and ligated into the pET32a expression plasmid. The MCP gene of OGNNV was 1 017 bases, encoded a protein of 338 amino acid with a molecular mass of 37.1 kDa. Recombinant protein with a molecular mass of 57.4 kDa was expressed in E.coli BL21(DE3). Vaccine was prepared from the recombinant protein expressed in recombinant cells. The juvenile orange-spotted groupers (8 cm in average length) were immunized by intraperitoneal injection. Group A was challenged with infected tissue filtrates 25 d post-vaccination. The mortality in the vaccined group (A1, 30%) was a little higher than the unvaccined group (B2, 27.8%). Group B was challenged after three vaccine injections. The mortality in the vaccined group (B1, 16.7%) was lower than the unvaccined group (B2, 27.8%), And the relative percentage survival (RPS) value of vaccined group, compared with the unvaccined group, was 40%. The anti-recombinant protein sera with a 1∶100 dilution were mixed with double volume of infected tissue filtrates and incubated at 4 ℃ for 12 h and then intramuscularly injected into the juvenile orange-spotted grouper. Treatment of infected tissue filtrates with anti-recombinant protein serum resulted in a significantly lower mortality of fish (Group C1, mortality of 18.18%), compared with the fish (Group C2, mortality of 40%) which received infected tissue filtrates treated with control serum. Results implied the potential use of the capsid protein in immunization against OGNNV. | CHEN Xiaoyan WENG Shaoping CHEN Shengping CHEN Zhaoming HE Jianguo | 2007 | Acta Oceanologica Sinica2007，26，1： | 2 |
| 5 | Surface relief grating from electrostatically layered azo dye films显示文摘 | Bian Shaoping | 2000 | Appl Phys Lett2000，76，22： | 1 |
| 6 | Characterization of a Tn MAVS protein from Tetraodon nigroviridis显示文摘 | Zhiming Xiang Lin Qi Weijian Chen Chuanfu Dong Zhaoyu Liu Dong Liu Mengli Huang Wei Li Gan Yang Shaoping Weng Jianguo He | 2011 | Developmental and Comparative Immunology2011，，11： | 1 |
| 7 | Symbolic vector dynamics approach to initial condition and control parameters estimation of coupled map lattices显示文摘 | WANG Kai PEI Wenjiang WANG Shaoping CHEUNG Yiuming HE Zhenya | 2008 | Circuits and Systems I: Regular Papers IEEE Transactions on2008，55，4： | 1 |
| 8 | Pseudoaneurysm with Median Nerve Injury Caused by Right Radial Artery Puncture:A Case Report显示文摘Background:Pseudoaneurysm with median nerve injury is a serious complication of radial artery puncture.It is very important to summarize the prevention and treatment experience of this complication through case discussion.Case report:A 66-year-old woman was admitted to the hospital because of‘‘paroxysmal chest tightness and suffocation for 5 days.”Coronary angiography was performed.During insertion of the arterial sheath,the patient experienced severe pain in the right forearm,which radiated to the palm.The puncture sheath did not return blood after the sheath core was withdrawn.The sheath was removed and local compression was used to stop bleeding.There was no obvious bleeding at the puncture point,and the compression was removed 6 hours after the procedure.Local swelling and increased tension were seen in the right forearm.At the 1-week follow-up she exhibited swelling,high local tension,small blisters,and bluish-purple skin of the right forearm,with an acceptable right radial artery pulsation.She had severe pain in the affected limb,which radiated to the thumb,index fi nger,and middle fi nger.Case discussion:We discuss the causes of and treatment measures for pseudoaneurysm with median nerve injury caused by radial artery puncture. | Yachao Li Zengming Xue He Ma Wei Zhang Shaoping Nie Changsheng Ma | 2021 | Cardiovascular Innovations and Applications2021，，1： | 0 |
| 9 | AbInitio Calculation of Structure and Raman and Infrared Spectra of Trifluoromethylthiosulphenyl Chloride显示文摘AbInitioCalculationofStructureandRamanandInfraredSpectraofTrifluoromethylthiosulphenylChlorideZouXianwu,LiuHaoyang,LuHongwenZ... | Wang Shaojie Wang Bo Li Shiqing Dai Yiqun He Chunqing Zhang Shaoping | 1997 | Wuhan University Journal of Natural Sciences1997，2，2： | 0 |
| 10 | Commissioning experiment of the high-contrast SILEX-Ⅱ multi-petawatt laser facility显示文摘The results of a commissioning experiment on the SILEX-Ⅱlaser facility(formerly known as CAEP-PW)are reported.SILEX-Ⅱis a complete optical parametric chirped-pulse amplification laser facility.The peak power reached about 1 PWin a 30 fs pulse duration during the experiment.The laser contrast was better than 1010 at 20 ps ahead of the main pulse.In the basic laser foil target interaction,a set of experimental data were collected,including spatially resolved x-ray emission,the image of the coherent transition radiation,the harmonic spectra in the direction of reflection,the energy spectra and beam profile of accelerated protons,hot-electron spectra,and transmitted laser energy fraction and spatial distribution.The experimental results show that the laser intensity reached 531020 W/cm^(2) within a 5.8μm focus(FWHM).Significant laser transmission did not occur when the thickness of theCHfoil was equal to or greater than 50 nm.The maximum energy of the accelerated protons in the target normal direction was roughly unchanged when the target thickness varied between 50 nm and 15μm.The maximum proton energy via the target normal sheath field acceleration mechanism was about 21 MeV.We expect the on-target laser intensity to reach 10^(22) W/cm^(2) in the near future,after optimization of the laser focus and upgrade of the laser power to 3 PW. | Wei Hong Shukai He Jian Teng Zhigang Deng Zhimeng Zhang Feng Lu Bo Zhang Bin Zhu Zenghai Dai Bo Cui Yuchi Wu Dongxiao Liu Wei Qi Jinlong Jiao Faqiang Zhang Zuhua Yang Feng Zhang Bi Bi Xiaoming Zeng Kainan Zhou Yanlei Zuo Xiaojun Huang Na Xie Yi Guo Jingqin Su Dan Han Ying Mao Leifeng Cao Weimin Zhou Yuqiu Gu Feng Jing Baohan Zhang Hongbo Cai Minqing He Wudi Zheng Shaoping Zhu Wenjun Ma Dahui Wang Yinren Shou Xueqing Yan Bin Qiao Yi Zhang Congling Zhong Xiaohui Yuan and Wenqing Wei | 2021 | Matter and Radiation at Extremes2021，6，6： | 0 |
| 11 | Bacterial and eukaryotic community interactions might contribute to shrimp culture pond soil ecosystem at different culture stages显示文摘Sedimentary bacterial and eukaryotic communities are major components of the aquatic ecosystem.Revealing the linkages between their community structure and interactions is crucial to understand the diversity and functions of aquatic and soil ecosystems.However,how their diversity and assembly contribute to their interactions on time scale is unclear.This study examined sedimentary bacterial and eukaryotic communities in shrimp culture ponds at different culture stages.The most abundant bacteria were Proteobacteria(38.27%),whereas the most abundant eukaryotes were Chytridiomycota(27.48%).Bacterial and eukaryotic diversities were correlated(P<0.05),implying the strong interactions between bacteria and eukaryotes.Results showed that the bacterial and eukaryotic communities became increasingly similar on a local scale along with the shrimp culture.Only the eukaryotic community significantly increased in similarity along with the shrimp culture(P<0.05),suggesting that the sedimentary eukaryotic community structure is sensitive under shrimp culture.Co-occurrence network modeling indicated that positive microbial interactions were dominant.The homogeneous selection was the major driver of community assembly.Bacterial diversity negatively correlated with operational taxonomic units and positive links in networks(P<0.05),whereas eukaryotic diversities positively correlated with positive links in networks(P<0.05).This study broadens our knowledge about sedimentary microbial diversity,community assembly,and interaction patterns on time scale,providing a reference for the sustainable management in aquaculture production. | Renjun Zhou Hao Wang Dongdong Wei Shenzheng Zeng Dongwei Hou Shaoping Weng Jianguo He Zhijian Huang | 2022 | Soil Ecology Letters2022，4，2： | 0 |
| 12 | Determination of Residues and Safety Analysis of Metal Impurities in Platinum-based Antitumor Drugs显示文摘The residual metal impurities in cisplatin, carboplatin and oxaliplatin were determined by ICP-AES. The samples were ignited and dissolved with HCl:HNO 3 (3:1). The method is simple and accurate. By the determination of the metal residues in the samples, the calculated actual daily exposure and concentration of the metal Pd, Ir, Rh, Ru, Mo, Ni, Cr, V, Cu, Mn, Fe and Zn that were less than the permitted daily exposures (PDE) and the limited concentration permitted in the EMEA guideline on the specification limits for residues of metal catalysts or metal reagents [1] . The metal residues can de adequately removed from the active pharmaceutical ingredients and the corresponding drugs. The trace metal residues will not affect human health and lead to the safety hazard by the intravenous injection. | PENG Juan PU Shaoping LI Guangli HE Jian ZHANG Zhirong LIAO Yunxing | 2012 | 贵金属2012，33，A01： | 0 |
| 13 | Correlation Function of Clumps for Electrons in Localized Coherent Langmuir Fields显示文摘A correlation function of electrons interacting with localized coherent Langmuir wave packets is obtained by particle simulation.It is shown that the peak behavior of the correlation function for sufficiently small relative coordinates r^(0) and u^(0) in phase space also occurs in a spatially inhomogeneous system,and the neighboring particles in phase space are correlated with each other for a lifetime exceeding the coherent time.Our numerical results display the clump effects,which are consistent with previous theoretical prediction of the relative diffusion behavior. | ZHOU Cangtao HE Xiantu ZHU Shaoping | 1992 | Chinese Physics Letters1992，9，8： | 0 |
| 14 | Functional genomics of Brassica napus:Progresses,challenges,and perspectives显示文摘Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil worldwide.Identification and understanding the gene functions in the B.napus genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B.napus.In this review,we present an overview of the progress made in the functional genomics of B.napus,including the availability of germplasm resources,omics databases and cloned functional genes.Based on the current progress,we also highlight the main challenges and perspectives in this field.The advances in the functional genomics of B.napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B.napus and will expedite the breeding of high quality,high resistance and high yield in B.napus varieties. | Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu Guangsheng Yang Tingdong Fu Liang Guo Hu Zhao | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
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