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| 1 | Nanoscale pore morphology and distribution of lacustrine shale reservoirs:Examples from the Upper Triassic Yanchang Formation,Ordos Basin显示文摘Pore structure plays an important role in the gas storage and flow capacity of shale gas reservoirs. Fieldemission environmental scanning electron microscopy(FE-SEM) in combination with low-pressure carbon dioxide gas adsorption(CO2GA),nitrogen gas adsorption(N2GA),and high-pressure mercury intrusion(HPMI) were used to study the nanostructure pore morphology and pore-size distributions(PSDs) of lacustrine shale from the Upper Triassic Yanchang Formation,Ordos Basin. Results show that the pores in the shale reservoirs are generally nanoscale and can be classified into four types: organic,interparticle,intraparticle,and microfracture. The interparticle pores between clay particles and organic-matter pores develop most often,l with pore sizes that vary from several to more than 100 nm. Mercury porosimetry analysis shows total porosities ranging between 1.93 and 7.68%,with a mean value of 5.27%. The BET surface areas as determined by N2 adsorption in the nine samples range from 10 to 20 m2/g and the CO2 equivalent surface areas(<2 nm)vary from 18 to 71 m2/g. Together,the HPMI,N2 GA,and CO2 GA curves indicate that the pore volumes are mainly due to pores <100 nm in size. In contrast,however,most of the specific surface areas are provided by the micropores. The total organic carbon(TOC) and clay minerals are the primary controls of the structures of nanoscale pores(especially micropores and mesopores). Micropores are predominantly determined by the content of the TOC,and mesopores are possibly related to the content of clay minerals,particularly the illite-montmorillonite mixed-layer content. | Yang Wang Yanming Zhu Hongyan Wang Guangjun Feng | 2015 | Journal of Energy Chemistry2015，24，4： | 6 |
| 2 | Association between CISH polymorphisms and spontaneous clearance of hepatitis B virus in hepatitis B extracellular antigen-positive patients during immune active phase显示文摘 | Song Guangjun Rao Huiying Feng Bo Wei Lai | 2014 | Chinese Medical Journal2014，，9： | 4 |
| 3 | Personalized cancer vaccines from bacteria-derived outer membrane vesicles with antibody-mediated persistent uptake by dendritic cells显示文摘Nanocarriers with intrinsic immune adjuvant properties can activate the innate immune system while delivering tumor antigen,thus efficiently facilitating antitumor adaptive immunity.Bacteria-derived outer membrane vesicles(OMVs)are an excellent candidate due to their abundance of pathogen associated molecular patterns.However,during the uptake of OMVs by dendritic cells(DCs),the interaction between lipopolysaccharide and toll-like receptor 4 induces rapid DC maturation and uptake blockage,a phenomenon we refer to as“maturation-induced uptake obstruction'(MUO).Herein we decorated OMV with the DC-targeting aDEC205 antibody(OMV-DEC),which endowed the nanovaccine with an uptake mechanism termed as 4 | Jie Liang Keman Cheng Yao Li Jiaqi Xu Yiwei Chen Nana Ma Qingqing Feng Fei Zhu Xiaotu Ma Tianjiao Zhang Yale Yue Guangna Liu Xinjing Guo Zhiqiang Chen Xinwei Wang Ruifang Zhao Ying Zhao Jian Shi Xiao Zhao Guangjun Nie | 2022 | Fundamental Research2022，2，1： | 2 |
| 4 | Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus( PRV) infection. [Method]SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus infection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently,the established assay was applied to detect different clinical samples. [Result]The sensitivity of SYBR Green I real-time quantitative PCR assay( 52 copies / μl) was 1 000 times higher than that of conventional PCR( 5. 2 × 104 copies / μl) and the detection time was shortened by 1 /2. The established assay could be used to detect PRV but could not be used to detect PCV2,PPV,CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain,nasal swab,inguinal lymph node,liver,lung and spleen was above 20,while PRV was not detected in the kidney and heart tissues. [Conclusion]The established SYBR Green I real-time quantitative PCR assay for PRV LAT detection was specific,sensitive and rapid,which could be used for pathogen monitoring,epidemiological investigation and quantitative study of PRV. | Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN | 2015 | Agricultural Biotechnology2015，4，3： | 2 |
| 5 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 6 | Hydrogen-rich gas from catalytic steam gasification of municipal solid waste (MSW): influence of catalyst and temperature on yield and product composition 显示文摘 | He Maoyun Hu Zhiquan Xiao Bo Li Jianfen Guo Xianjun Luo Siyi Yang Fan Feng Yu Yang Guangjun Liu Shiming | 2009 | International Journal of Hydrogen Energy2009，34，1： | 1 |
| 7 | Effects of Chinese herbal cataplasm Xiaozhang Tie on cirrhotic ascites显示文摘 | Xing Feng Tan Ye Yan GuangJun | 2012 | J Ethnopharmacol2012，139，2： | 1 |
| 8 | Identification Methods for Hammerstein Nonlinear Systems显示文摘 | Ding Feng Liu Xiaoping Liu Guangjun | 2011 | Digital Signal Processing2011，21，2： | 1 |
| 9 | Parameter estimation with scarce measurements显示文摘 | Feng Ding Guangjun Liu Xiaoping P. Liu | 2011 | Automatica2011，，8： | 1 |
| 10 | Auxiliary model based multi-innovation extended stochastic gradient parameter estimation with colored measurement noises显示文摘 | Feng Ding Peter X. Liu Guangjun Liu | 2009 | Signal Processing2009，，10： | 1 |
| 11 | Identification Methods for Hammerstein Nonlinear Systems 显示文摘 | Ding Feng Liu Xiaoping Peter Liu Guangjun | 2011 | Digital Signal Processing2011，21，2： | 1 |
| 12 | A new comparison theorem and the solvability of a third-order two-point boundary value problem 显示文摘 | Feng Yuqiang Qu Guangjun | 2011 | Bull Malays Math Sci Soc2011，34，3： | 1 |
| 13 | Anatase TiO2 nanoparticles/carbon nanotubes nanofibers: preparation, characterization and photocatalytic properties显示文摘 | Guangjun Hu Xiangfu Meng Xiyan Feng Yanfen Ding Shimin Zhang Mingshu Yang | 2007 | Journal of Materials Science2007，，17： | 1 |
| 14 | Highly sensitive electrochemical determination of rutin based on the synergistic effect of 3D porous carbon and cobalt tungstate nanosheets显示文摘Rutin,a flavonoid found in fruits and vegetables,is a potential anticancer compound with strong anticancer activity.Therefore,electrochemical sensor was developed for the detection of rutin.In this study,CoWO_(4) nanosheets were synthesized via a hydrothermal method,and porous carbon(PC)was prepared via high-temperature pyrolysis.Successful preparation of the materials was confirmed,and characterization was performed by transmission electron microscopy,scanning electron microscopy,and X-ray photoelectron spectroscopy.A mixture of PC and CoWO_(4) nanosheets was used as an electrode modifier to fabricate the electrochemical sensor for the electrochemical determination of rutin.The 3D CoWO_(4) nanosheets exhibited high electrocatalytic activity and good stability.PC has a high surface-to-volume ratio and superior conductivity.Moreover,the hydrophobicity of PC allows large amounts of rutin to be adsorbed,thereby increasing the concentration of rutin at the electrode surface.Owing to the synergistic effect of the 3D CoWO_(4) nanosheets and PC,the developed electrochemical sensor was employed to quantitively determine rutin with high stability and sensitivity.The sensor showed a good linear range(5-5000 ng/mL)with a detection limit of 0.45 ng/mL.The developed sensor was successfully applied to the determination of rutin in crushed tablets and human serum samples. | Guangjun Feng Yang Yang Jiantao Zeng Jun Zhu Jingjian Liu Lun Wu Zhiming Yang Guanyi Yang Quanxi Mei Qinhua Chen Fengying Ran | 2022 | Journal of Pharmaceutical Analysis2022，12，3： | 1 |
| 15 | Multiinnovation least- squares identification for system modeling显示文摘 | DING Feng LIU Xiaoping LIU Guangjun | 2010 | IEEE Transactions on Systems Man and Cybernetics Part B : Cybernetics2010，40，3： | 1 |
| 16 | Analysis of New Materials Application in Municipal Road Construction显示文摘In order to promote the development of road traffic in our country,it is necessary to strengthen the research on municipal road construction technology,constantly innovate construction technology and construction technique,and then effectively ensure the rapid development of urban traffic.This paper mainly elaborates on asphalt road surface regeneration technology,modified asphalt concrete,concrete road surface anti-cracking technology,three-dimensional printing technology,composite material road surface,polymer composite materials,etc.,to ensure the development of urban transportation and the quality of municipal road projects. | Guangjun Li Lu Zhang Lin Nan Manman Feng Shuyan Liu | 2023 | Journal of Architectural Research and Development2023，7，3： | 0 |
| 17 | A Study of 90°Ferroelectric Domains in Potassium Tantalate Niobate Crystals by Means of Transmission Electron Microscopy显示文摘By flux method,potassium tantalate niobate crystals with 46%Nb contents were prepared.In this material 90°a-type domains with(011)and(01ī)walls were observed by a transmission electron microscope,and the nature of fringe patterns associated with these domain walls was analysed.According to the extinction rules for the domain walls,the displacement vector R along<01ī>direction can be determined.Finally,using a selected area electron diffraction method,we examined the crystallographic character of these 90°domains and defined the ratio of lattice parameters c to a. | XU Huiping LI Qi SHEN Guangjun WANG Luchun SHA Zhenshun ZHU Jianming FENG Duan WANG Jiyang | 1993 | Chinese Physics Letters1993，10，5： | 0 |
| 18 | Ridge Covering High-yield Cultivation Techniques of Jinhong Apple in Cold Region显示文摘After five years of study,it was found that compared with the conventional cultivation techniques,the average yield per plant of3-5-year-old Jinhong apple trees cultivated under the ridge covering mode was increased by 2. 12 times,the stem diameter was increased by20%,the branch number was increased by 73. 5%,and the underground root number was increased by 38. 3%. Under the ridge covering mode,the main distribution area of the underground roots of Jinhong apple trees was moved down to 15-55 cm,the temperature of soil 20 cm beneath the surface of the ridges increased by 1. 3-1. 5℃,the temperature of soil 40 cm beneath the surface of the ridges rose by 0. 9-1. 0℃,and the water content of soil 20-40 cm from the ground was maintained around 5%. Under the ridge covering mode,the Jinhong apple trees grew robustly,began to fruit 3 years later and yielded highly 5 years later. In addition,the ridge covering model also has illumination-enhancing,pest-controlling and tillage-free characteristics. | Chang LIU Zhangli FENG Siyang TANG Haidong BU Minggang XIAO Guangjun GU Xianmin CHENG Wenquan YU Wanjun YE | 2019 | Asian Agricultural Research2019，11，4： | 0 |
| 19 | Research on Infrared Image Fusion Technology Based on Road Crack Detection显示文摘This study aimed to propose road crack detection method based on infrared image fusion technology.By analyzing the characteristics of road crack images,this method uses a variety of infrared image fusion methods to process different types of images.The use of this method allows the detection of road cracks,which not only reduces the professional requirements for inspectors,but also improves the accuracy of road crack detection.Based on infrared image processing technology,on the basis of in-depth analysis of infrared image features,a road crack detection method is proposed,which can accurately identify the road crack location,direction,length,and other characteristic information.Experiments showed that this method has a good effect,and can meet the requirement of road crack detection. | Guangjun Li Lin Nan Lu Zhang Manman Feng Yan Liu Xu Meng | 2023 | Journal of World Architecture2023，7，3： | 0 |
| 20 | Research Progress in Prevention and Treatment of Bacterial Diseases by Traditional Chinese Medicine显示文摘Recently,antibiotics have been widely used to resist various bacterial diseases in livestock breeding,which generated more superbacteria with resistance. Food safety problems,such as animal drug residues are urgent to be solved. Traditional Chinese medicines are traditional treasures of China,which attract more and more attention in the prevention and treatment of bacterial diseases because of their abundant advantages including improving immunologic function of organisms,antibacterial,antivirus,antitumor,anti-inflammatory and improving animal performance. The advantages of traditional Chinese medicines,such as low toxicity,low residual,green and safe,conform to food safety requirements. The using condition of traditional Chinese medicines in main bacterial diseases of animals was reviewed in the paper,thus to provide a reference for livestock production. | Sufang LYU Guangjun GUO Ling MO Feng LI Feng WEI Bingmei DONG Zhiqiang SHEN | 2016 | Agricultural Biotechnology2016，5，4： | 0 |
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