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您的检索式：作者名="Qu Guangjun"
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| 1 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 2 | A new comparison theorem and the solvability of a third-order two-point boundary value problem 显示文摘 | Feng Yuqiang Qu Guangjun | 2011 | Bull Malays Math Sci Soc2011，34，3： | 1 |
| 3 | Induced Neural Stem Cells Generated from Rat Fibroblasts显示文摘The generation of induced tissue-specifc stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specifc stem cells like the ones we have for embryonic stem(ES)cell cultures.Using a cocktail of cytokines and small molecules,we demonstrate that primitive neural stem(NS)cells derived from mouse ES cells and rat embryos can be maintained.Furthermore,using the same set of cytokines and small molecules,we show that induced NS(iNS)cells can be generated from rat fbroblasts by forced expression of the transcriptional factors Oct4,Sox2 and c-Myc.The generation and long-term maintenance of iNS cells could have wide and momentous implications. | Guangjun Xi Pingfang Hu Cunye Qu Shenfeng Qiu Chang Tong Qi-Long Ying | 2013 | Genomics, Proteomics & Bioinformatics2013，11，5： | 1 |
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