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| 1 | A delivery strategy for rotenone microspheres in an animal model of Parkinson's disease.显示文摘 | Huang J Liu H Gu W Yan Z Xu Z Yang Y Zhu X Li Y | 2006 | 中国生物学文摘2006，20，9： | 16 |
| 2 | Structural basis of AMPK regulation by adenine nucleotides and glycogen显示文摘激活安培的蛋白质 kinase (AMPK ) 为糖尿病,肥胖,和癌症的治疗是一个中央细胞的精力传感器和精力动态平衡,和一个有希望的药目标的管理者。这里我们人的 α 的现在的低分辨率的水晶结构; 1 β 2 γ 1 holo-AMPK 建筑群跳了到它的 allosteric 调节的人安培和肝糖模仿 cyclodextrin,两个在 phosphorylated (4.05 Å) 并且 non-phosphorylated (4.60 Å) 状态。另外,我们解决了 2.95 Å人的 kinase 领域(KD ) 的结构跳了到邻近的 autoinhibitory 领域(帮助) 并且表现了广泛生物化学并且 mutational 研究。一起,这些研究由安培和肝糖说明 allosteric AMPK 调整的内在的机制,其绑定改变在交替的帮助(安培) 和糖类绑定模块(肝糖) 之间的 equilibria 相互作用。 | Xiaodan Li Lili Wang X Edward Zhou Jiyuan Ke Parker W de Waal Xin Gu M H Eileen Tan Dongye Wang Donghai Wu H Eric Xu Karsten Melcher | 2015 | Cell Research2015，25，1： | 8 |
| 3 | Directly patterned substrate-free plasmonic “nanograter” structures with unusual Fano resonances显示文摘The application of three-dimensional(3D)plasmonic nanostructures as metamaterials(MMs),nano-antennas,and other devices faces challenges in producing metallic nanostructures with easily definable orientations,sophisticated shapes,and smooth surfaces that are operational in the optical regime and beyond.Here,we demonstrate that complex 3D nanostructures can be readily achieved with focused-ion-beam irradiation-induced folding and examine the optical characteristics of plasmonic“nanograter”structures that are composed of free-standing Au films.These 3D nanostructures exhibit interesting 3D hybridization in current flows and exhibit unusual and well-scalable Fano resonances at wavelengths ranging from 1.6 to 6.4 mm.Upon the introduction of liquids of various refractive indices to the structures,a strong dependence of the Fano resonance is observed,with spectral sensitivities of 1400 nm and 2040 nm per refractive index unit under figures of merit of 35.0 and 12.5,respectively,for low-order and high-order resonance in the near-infrared region.This work indicates the exciting,increasing relevance of similarly constructed 3D free-standing nanostructures in the research and development of photonics and MMs. | Ajuan Cui Zhe Liu Jiafang Li Tiehan H Shen Xiaoxiang Xia Zhiyuan Li Zhijie Gong Hongqiang Li Benli Wang Junjie Li Haifang Yang Wuxia Li Changzhi Gu | 2015 | Light(Science & Applications)2015，4，1： | 8 |
| 4 | 流感病毒传播能力相关遗传机制的深度解析显示文摘在人与人之间通过呼吸道高效传播的能力是新发流感病毒引起人流感大流行的先决条件,但流感病毒传播能力遗传决定因素至今并不完全清楚。揭示流感病毒传播能力遗传机制对人流感大流行的预警以及防控疫苗和药物研发都至关重要,因此一直是病毒学领域研究的重点。2013年出现在我国的H7N9亚型禽流感病毒已导致1560多人感染,致死率近40%。 | Kong H Ma S Wang J Gu C | 2019 | 中国预防兽医学报2019，41，11： | 5 |
| 5 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 6 | 连续油管油,气井排液瞬时特性显示文摘运用连续油管携带氮气,循环井筒,进行油,气井排液的过程是一个瞬变过程。在这一排液过程中,井筒内最初的较重的流体被氮气和较轻的油藏流体驱替出来。在确定油,气井排液所需氮气量及所需施工时间时,需要考虑排液时井筒的动态变化。 | Gu,H 韩群业 | 1997 | 采油工艺情报1997，，4： | 4 |
| 7 | Structural basis of the Norrin-Frizzled 4 interaction显示文摘 | Guobo Shen Jiyuan Ke Zhizhi Wang Zhihong Cheng Xin Gu Yuquan Wei Karsten Melcher H Eric Xu Wenqing Xu | 2015 | Cell Research2015，25，9： | 3 |
| 8 | Pharmacokinetics of eugenol in rainbow trout ( Oncorhynchus mykiss )显示文摘 | S.A. Guénette F.C. Uhland P. Hélie F. Beaudry P. Vachon | 2007 | Aquaculture2007，，1： | 2 |
| 9 | Magnetically recoverable nanoparticles:highly efficient catalysts for asymmetric transfer hydrogenation of aromatic ketones in aqueous medium显示文摘 | LIU G GU H SUN Y | | 0，，08： | 2 |
| 10 | Magnetically recoverable SiO2-coated Fe3O4 nanoparticles:a new platform for asymmetric transfer hydrogenation of aromatic ketones in aqueous medium显示文摘 | SUN Y LIU G GU H | | 0，，09： | 2 |
| 11 | Generation and characterization of a human nanobody against VEGFR-2显示文摘瞄准:Nanobody 是由一个单个 monomeric 变量抗体领域组成的抗体碎片,它能被用于许多 biotechnological 和治疗学的目的。这个工作的目的是从噬菌体显示器 library.Methods 对 VEGFR-2 domain3 (VEGFR D3 ) 孤立并且描绘人的信号域抗体:与与 VEGFR2 D3 的高亲密关系生产抗原特定的 recombinant nanobodies,液体阶段 panning 策略被用于 panning 的 all rounds。为 nanobody 表示和纯化,四 VEGFR2 堵住 D3 克隆是进 pETduet-biotin-MBP 表示向量的 subcloned。recombinant 蛋白质带了一个 MBP 标签由亲密关系层析便于纯化。Recombinant NTV (1-4 ) 在另外的胶化过滤层析步以后被获得。在 VEGFR2 D3 和 NTV (1-4 ) 之间的相互作用与基于光的 AlphaScreen 试金和 SPR 试金被估计。Anti-angiogenesis 效果在人的脐的静脉 endothelial 房间(HUVEC ) 被检验 .Results:在 AlphaScreen 试金, NTV1 (100 和 200 nmol/L ) 与 VEGFR2 D3 得到了最高有约束力的信号;NTV2 与 VEGFR2 D3 显示出中等相互作用;NTV3 和 NTV4 展出了有 VEGFR2 D3 的很少或没有相互作用。在 SPR 试金, NTV1 与一个平衡分离常数为 VEGFR2 D3 显示了一种高亲密关系(K 49 ± 的 D ) ; 1.8 nmol/L。NTV1 (1-1000 nmol/L ) dose-dependently 由 HUVECs.Conclusion 禁止了 HUVEC 和 endothelial 试管形成的增长:nanobody NTV1 是堵住 VEGFR2 的一个潜在的治疗学的候选人。这研究为指向 VEGFR2 的基于 nanobody 的癌症治疗学的开发提供新奇、有希望的策略。 | Lin MA Kai GU Cheng-hai ZHANG Xue-tao CHEN Yi JIANG Karsten MELCHER Juan ZHANG Min WANG H Eric XU | 2016 | Acta Pharmacologica Sinica2016，37，6： | 2 |
| 12 | The Reaction of Benzimidazole Methiodide Salt with Bis-Grignard Reagent显示文摘The reaction of benzimidazole methoidide solt with bis-Grignard reagent is reported,and a new method for the preparation of dialdehyde is | Shi, Z Gu, H | 1997 | Chinese Chemical Letters1997，8，6： | 2 |
| 13 | Introduction and constitutive expression of rice chitinase gene in bread wheat using biolistic bombardment and the bar gene as a selectable maker显示文摘 | Chen W P Gu X Liang G H Muthukrishnan S Chen P D Liu D J Giu B S | 1998 | Theor Appl Genet1998，97，12： | 2 |
| 14 | Secular change of annual and interannual variability in the tropics during the past century 显示文摘 | Gu D F Phlander S G H | 1995 | J Climate1995，8，4： | 2 |
| 15 | Studies on the origin and evolution of tetraploid wheats on the internal transcribed (ITS) sequences of nuclear ribosomal DNA显示文摘 | Zhang W Qu L Gu H | 2002 | TAG Theoretical and Applied Genetics2002，104，67： | 1 |
| 16 | Parity anomaly of bound states and optical properties in semiconductor supcrlattices with structural defects 显示文摘 | WANG X H GU B Y YANG G Z | 1998 | Phys Rev B1998，58，8： | 1 |
| 17 | Delamination buckling and postbuekling of composite cylindrical shells 显示文摘 | Chattopadhyay A Gu H | 1996 | AIAA Journal1996，34，6： | 1 |
| 18 | Hypoxia-induced increase in soluble Flt-1 production correlates with enhanced oxidative stress in trophoblast cells from the human placenta显示文摘 | Gu B Zhang Y | 2005 | Placenta2005，26，： | 1 |
| 19 | Free-form surface inspection techniques state of the art review显示文摘 | LI Y D GU P H | 2004 | Computer-Aided Design2004，36，13： | 1 |
| 20 | Cre-loxP-mediated gene replacement:a mouse strain producing humanized antibodies显示文摘 | Zou Y R Müller W Gu H | 1994 | Curr Biol1994，4，12： | 1 |
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