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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Pharmacokinetics of eugenol in rainbow trout ( Oncorhynchus mykiss )显示文摘 | S.A. Guénette F.C. Uhland P. Hélie F. Beaudry P. Vachon | 2007 | Aquaculture2007，，1： | 2 |
| 3 | Genetic diversity of red-fleshed apples (Malus)显示文摘 | Steven Nocker Garrett Berry James Najdowski Roberto Michelutti Margie Luffman Philip Forsline Nihad Alsmairat Randy Beaudry Muraleedharan Nair Matthew Ordidge | 2012 | Euphytica2012，，2： | 2 |
| 4 | Case selection in minimally invasive surgical treatment of neuroblastoma显示文摘 | Boutros J Bond M Beaudry P | 2008 | Pediatr Surg Int2008，24，10： | 1 |
| 5 | Lipids and stroke: a paradox resolved 显示文摘 | Hachinski V Graffagnino C Beaudry M | 1996 | Arch Neurol1996，53，4： | 1 |
| 6 | The Other Side of Acceptance:Studying the Direct and Indirect Effects of Emotions on Information Technology Use显示文摘 | BEAUDRY A PINSONNEAULT A | 2010 | MIS Quarterly2010，34，4： | 1 |
| 7 | Differential effects of vascular endothelial growth factor receptor-2 inhibitor ZD6474 on circulating endothelial progenitors and mature circulating endothelial cells:implications for use as a surrogate marker of antiangiogenic activity显示文摘 | Beaudry P Force J Naumov GN | 2005 | Clin Cancer Res2005，11，9： | 1 |
| 8 | Do Recessions Permanently Change Output? 显示文摘 | BEAUDRY P KOOP G | 1993 | Journal of Monetary Economics1993，31，： | 1 |
| 9 | Pressure dependence of the Fermi surface of hcp ytterbium显示文摘 | Jepsen O Schirber J E Beaudry B J | 1981 | Physical Review B1981，，23： | 1 |
| 10 | Isolation of differentially expressed genes in concep-tuses and endometrial tissue of sows in early gestation显示文摘 | VALLEE M BEAUDRY D ROBERGE C | 2003 | Biol Reprod2003，69，5： | 1 |
| 11 | Membrane technology for direct osmosis concentration of fruit juices 显示文摘 | BEAUDRY E G LAMPI K A | 1990 | J Food Technol1990，44，： | 1 |
| 12 | Monetary Instability, the Predictability of Prices, and the Allocation of Investment: An Empirical Investigation Using UK Panel Data显示文摘 | Beaudry P Caglayan M Schiantarelli F | 2001 | American Economic Review2001，91，3： | 1 |
| 13 | Total liquid ventilation efficacy in an ovine model of severe meconium aspiration syndrome 显示文摘 | AVOINE O BOSSE D BEAUDRY B | 2011 | Crit Care Med2011，39，5： | 1 |
| 14 | Effects of breed, parity, and folic acid supplement on the expression of folate metabolism genes in endometrial and embryonic tissues from sows in early pregnancy 显示文摘 | Vallee M Guay F Beaudry D | 2002 | Biol Reprod2002，67，： | 1 |
| 15 | An exploration into Pigou’s theory of cycles显示文摘 | Paul Beaudry Franck Portier | 2004 | Journal of Monetary Economics2004，，6： | 1 |
| 16 | The preparation of SiGe thermoelectric materials by mechanical mlloying显示文摘 | Cook B A Beaudry B J Harringa W J | 1989 | Institute of Electrical and Electronics Engineers1989，89，3： | 1 |
| 17 | Migrating glioma cells activate the P13-K pathway and display decreased susceptibility to apoptosis显示文摘 | Beaudry CE Tran NL | 2003 | J Cell Sci2003，116，21： | 1 |
| 18 | 14-3-3 protein,neuron-specific enolase,and S-100 protein in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease显示文摘 | Beaudry P | 1999 | Dement Geriatr Cogn Disord1999，10，： | 1 |
| 19 | Forwardosmosis treatment of drilling mud and fracturing wastewater from oil and gas operations显示文摘 | Appleton E W Beaudry E G Xu P | 2013 | Desalination2013，，312： | 1 |
| 20 | An Exploration into Pigou's Theory of Cycles 显示文摘 | Paul Beaudry Franck Portier | 2004 | Journal of Monetary Economics2004，51，： | 1 |
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